• Journal of the Korean Geotechnical Society
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• v.36 no.10
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• pp.5-19
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• 2020

With increasing demand for offshore structures, the demand for temporary structures to help the offshore construction work has increased. A cofferdam is a temporary barrier to stop the inflow of water in the construction site and allows working in the dry condition when the construction is done within the water. However, it is a major cause of construction delays and increased costs because additional works are required to block the water inflow. Recently, in order to overcome the limitations of the conventional cofferdam methods and to increase economic efficiency, a large-diameter steel cofferdam method has been proposed which can be installed quickly in the seabed by using the suction pressure. In this circular steel cofferdam method, the top side of the cofferdam including the top-lid is always exposed above the sea level in order to use it as a water barrier, unlike the conventional suction bucket foundation. After installation, the top-lid of the cofferdam is removed and the water filled inside the cofferdam is discharged to make the interior dry condition. In this study, the circular steel cofferdam with a 5 m inner diameter was fabricated and the installation tests were conducted at the Saemaguem test site. During the experiment, variation of suction pressure, leakage between connections, structure deformation, and inclination of the steel cofferdam were measured and post-analyzed. This study verified the new circular steel cofferdam method and confirmed that the suction installation method can be successfully used for various purposes on offshore structures.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.661-667
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• 2016

This study evaluated the potential of methodology development of the helical type anchor for soft ground applications. The rotational penetration of the helix structure might make construction-steps easy without the soil spitting and reusable rods could reduce the material cost. Removal of the anchors would be simple as a construction, which can be named the removal anchor. The anchoring resistance after construction is strongly related to the number of helixes resulting in a concise design process. The investigation involved a chamber test with soft soils. In the test, a specially designed and fabricated helical anchor and torque-driver were used to obtain the maximum pull-out resistance of the anchor after rotational penetration. As a result of the tests, The rotational torque and pull-out resistance have a proportional relationship with the strength of the prepared soils. Within the range of the study, the torque of the anchor penetrating increased with increasing pull-out resistance.

• Animal Bioscience
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• v.37 no.1
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• pp.131-141
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• 2024

Objective: This experiment aimed to explore the protective action of dietary supplementation with isoquinoline alkaloids (IA) from Macleaya cordata on lipopolysaccharide (LPS)-induced liver injury in broilers. Methods: Total 216 healthy broilers were selected in a 21-d trial and assigned randomly to the following 3 treatments: control (CON) group, LPS group, and LPS+IA group. The CON and LPS groups were provided with a basal diet, whereas the LPS+IA group received the basal diet supplemented with 0.6 mg/kg Macleaya cordata IA. Broilers in LPS and LPS+IA groups were intraperitoneally injected with LPS (1 mg/kg body weight) at 17, 19, and 21 days of age, while those in CON group were injected with equivalent amount of saline solution. Results: Results showed LPS injection caused systemic and liver inflammation in broilers, inhibited immune function, and ultimately lead to liver injury. By contrast, supplementation of IA ameliorated LPS-induced adverse change in serum parameters, boosted immunity in LPS+IA group. Furthermore, IA suppressed the elevation of hepatic inflammatory cytokines and caspases levels induced by LPS, as well as the expressions of genes related to the toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)/nuclear factor-kappa B (NF-κB) pathway. Conclusion: Dietary inclusion of 0.6 mg/kg Macleaya cordata IA could enhance immune function of body and inhibit liver damage via inactivating TLR4/MyD88/NF-κB signaling pathway in broilers.

• Smart Structures and Systems
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• v.16 no.2
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• pp.243-262
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• 2015

Optimal sensor placement (OSP) is a critical issue in construction and implementation of a sophisticated structural health monitoring (SHM) system. The uncertainties in the identified structural parameters based on the measured data may dramatically reduce the reliability of the condition evaluation results. In this paper, the information entropy, which provides an uncertainty metric for the identified structural parameters, is adopted as the performance measure for a sensor configuration, and the OSP problem is formulated as the multi-objective optimization problem of extracting the Pareto optimal sensor configurations that simultaneously minimize the appropriately defined information entropy indices. The nondirective movement glowworm swarm optimization (NMGSO) algorithm (based on the basic glowworm swarm optimization (GSO) algorithm) is proposed for identifying the effective Pareto optimal sensor configurations. The one-dimensional binary coding system is introduced to code the glowworms instead of the real vector coding method. The Hamming distance is employed to describe the divergence of different glowworms. The luciferin level of the glowworm is defined as a function of the rank value (RV) and the crowding distance (CD), which are deduced by non-dominated sorting. In addition, nondirective movement is developed to relocate the glowworms. A numerical simulation of a long-span suspension bridge is performed to demonstrate the effectiveness of the NMGSO algorithm. The results indicate that the NMGSO algorithm is capable of capturing the Pareto optimal sensor configurations with high accuracy and efficiency.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1531-1537
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• 2014

Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene whose reduced expression may play an important role in the development and progression of esophageal squamous cell cancer (ESCC). The aim of this study was to investigate the clinical relevance of RUNX3 in ESCC patients and effects of overexpression on biological behaviour of Eca109 cells in vitro and in vivo. Immunohistochemistry was performed to detect the clinical relevance of RUNX3 and lymph node metastasis in 80 ESCC tissues and 40 non-cancerous tissues using the SP method. RT-PCR and Western blotting were applied to assess the RUNX3 level and verify the Eca109 cell line with stable overexpression. Localization of RUNX3 proteins was performed by cell immunofluorescence. CCK-8 and Scrape motility assays were used to determine proliferation and migration and the TUNEL assay to analyze cell apoptosis. Invasive potential was assessed in cell transwell invasion experiments. In nude mice, tumorigenesis in vivo was determined. Results showed decreased expression of RUNX3 in esophageal tissue to be significantly related to lymph node metastasis (LNM) (P<0.01). In addition, construction of a recombinant lentiviral vector and transfection into the human ESCC cell line Eca109 demonstrated that overexpression could inhibit cell proliferation, migration and invasion, and induce apoptosis. The in vivo experiments in mice showed tumorigenicity and invasiveness to be significantly reduced. Taken together, our studies indicate that underexpression of RUNX3 in human ESCC tissue is significantly correlated with progression. Restoration of RUNX3 expression significantly inhibits ESCC cells proliferation, migration, invasion and tumorigenesis.

• BMB Reports
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• v.38 no.4
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• pp.414-419
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• 2005

The production of recombinant antibodies has been generally recognized as time-consuming and labor-intensive. The aim of our study is to construct mammalian expression vectors containing the cDNA encoding the human constant regions and murine variable regions to massively and cost-effectively produce full-length chimeric antibodies. Unique restriction sites flanking the Ig variable region were designed to allow for the replacement of variable regions generated by PCR. Western blot analysis of the chimeric antibodies revealed that the expressed products were of the predicted size, structure and specificity. The usefulness of the vectors was confirmed by construction of human-mouse chimeric antibody-HCAb which secretes murine antibody against the human colorectal cancer. Selected in medium containing gradually increasing methotrexate (MTX), clones with increased expression of the product gene can be efficiently generated. The secretion of recombinant chimeric antibody-HCAb yielded $30\;pg\;cell^{-1}\;day^{-1}$ at $10^{-6}\;M$ MTX. With this high-level expression from pools, the convenient and rapid production of over 100 milligram amounts per liter of recombinant antibodies may be achieved, which indicates the significant roles of pYR-GCEVH and pYR-GCEVL in the production of chimeric antibodies.

• Structural Engineering and Mechanics
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• v.37 no.6
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• pp.671-684
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• 2011

Deciding on an optimal sensor placement (OSP) is a common problem encountered in many engineering applications and is also a critical issue in the construction and implementation of an effective structural health monitoring (SHM) system. The present study focuses with techniques for selecting optimal sensor locations in a sensor network designed to monitor the health condition of Dalian World Trade Building which is the tallest in the northeast of China. Since the number of degree-of-freedom (DOF) of the building structure is too large, multi-modes should be selected to describe the dynamic behavior of a structural system with sufficient accuracy to allow its health state to be determined effectively. However, it's difficult to accurately distinguish the translational and rotational modes for the flexible structures with closely spaced modes by the modal participation mass ratios. In this paper, a new method of the OSP that computing the mode shape matrix in the weak axis of structure by the simplified multi-DOF system was presented based on the equivalent rigidity parameter identification method. The initial sensor assignment was obtained by the QR-factorization of the structural mode shape matrix. Taking the maximum off-diagonal element of the modal assurance criterion (MAC) matrix as a target function, one more sensor was added each time until the maximum off-diagonal element of the MAC reaches the threshold. Considering the economic factors, the final plan of sensor placement was determined. The numerical example demonstrated the feasibility and effectiveness of the proposed scheme.

• Proceedings of the SAREK Conference
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• 2008.11a
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• pp.611-617
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• 2008

Nowadays Plate Heat Exchanger (PHE) is widely used in different industries such as chemical, food and pharmaceutical process and refrigeration due to the efficient heat transfer performance, extreme compact design and efficient use of the construction material. In present study, discussed main conception of plate heat exchanger and applied in vacuum. PHE and aimed apply in the fresh water generator which installed in ship to desalinate seawater to fresh water use heat from engines. The experiment is proceeded to investigate the heat transfer between cold and hot fluid stream at different flow rate and supply temperature of hot fluid. Generated fresh water as outcome of the system. PHE is an important part of a condensing or evaporating system. One of common assumptions in basic heat exchanger design theory is that fluid is to be distributed uniformly at the inlet of each fluid side and throughout the core. However, in practice, flow mal-distribution is more common and can significantly reduce the heat exchanger performance. The flow and heat transfer are simulated by the k-$\varepsilon$ standard turbulence model. Moreover, the simulation contacted flow maldistribution in a PHE with 6 channels.

• Korean Institute of Interior Design Journal
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• v.21 no.3
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• pp.144-153
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• 2012

This study analyzed and investigated problems of local safety experience halls' spatial structure and media status. Improvements would be suggested after comprehending the need to execute safety education based on citizen's satisfaction and requests. Contents of safety experience space concept, display characteristics and types applied to local institution were analyzed. Safety experience centers with systemized protocols that opened after 2003 and were bigger than $5,000m^2$ were selected. We had three candidates and they were Seoul Gwangnaru Safety Experience Center, Boramea Safety Experience Center and Daegu Safety Theme Park. Going through checklists and interviewing official institutions that were in charge of safety hall educations helped comprehension of sample space status and detailed experimental presentation media. A survey was created based on this study and was distributed to users and non-users of safety experience halls. The survey consisted of general questions covering situations, awareness, level of interests, frequency and satisfaction level. The results were extracted with SPSS 18.0 through frequency analysis and cross tabulations. This research improvements through synthesized contemplation. For the resuits First, various experimental programs need to be supplemented in order to prevent accidents. Second, vivid experimental spaces need to be planned for active participation and users should be fully aware of safety hazard through education. Third, there weren't enough institutions compared to the education needs. Promoting and building more safety experience halls are dire. Finally, these suggestions of the results will be distributed in the planning of the exhibit hall.

• Wind and Structures
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• v.29 no.4
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• pp.235-246
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• 2019

Wind loading is one of important loadings that should be considered in the design of large hyperbolic natural-draught cooling towers. Both external and internal surfaces of cooling tower are under the action of wind loading for cooling circulating water. In the previous studies, the wind loads on the external surface attracted concernedly attention, while the study on the internal surface was relatively ware. In the present study, the wind pressure on the internal surface of a 220 m high cooling tower is measured through wind tunnel testing, and the effect of ventilation rate of the packing layer on internal pressure is a major concern. The characteristics of internal wind pressure distribution and its effect on wind-induced responses calculated by finite element method are investigated. The results indicate that the wind loading on internal surface of the cooling tower behaves remarkable three-dimensional effect, and the pressure coefficient varies along both of height and circumferential directions. The non-uniformity is particularly strong during the construction stage. Analysis results of the effect of internal pressure on wind-induced responses show that the size and distribution characteristics of internal pressure will have some influence on wind-induced response, however, the outer pressure plays a dominant role in the wind-induced response of cooling tower, and the contribution of internal pressure to the response is small.