• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1977-1981
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• 2012

Houttuynia cordata Thunb (HCT) is a native herb found in Southeast Asia which features various pharmacological activities against allergy, inflammation, viral and bacterial infection, and cancer. The aims of this study were to determine the cytotoxic effect of 6 fractions obtained from silica gel column chromatography of alcoholic HCT extract on human leukemic Molt-4 cells and demonstrate mechanisms of cell death. Six HCT fractions were cytotoxic to human lymphoblastic leukemic Molt-4 cells in a dose-dependent manner by MTT assay, fraction 4 exerting the greatest effects. Treatment with $IC_{50}$ of HCT fraction 4 significantly induced Molt-4 apoptosis detected by annexinV-FITC/propidium iodide for externalization of phosphatidylserine to the outer layer of cell membrane. The mitochondrial transmembrane potential was reduced in HCT fraction 4-treated Molt-4 cells. Moreover, decreased expression of Bcl-xl and increased levels of Smac/Diablo, Bax and GRP78 proteins were noted on immunoblotting. In conclusion, HCT fraction 4 induces Molt-4 apoptosis cell through an endoplasmic reticulum stress pathway.

• 동의생리병리학회지
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• 제20권6호
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• pp.1584-1592
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• 2006

Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is traditional medicinal plant generally used in Oriental medicine therapy. We previously reported that the water extract of H. cordata inhibited cell proliferation and induced apoptosis in human breast carcinoma cells. In the present study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of H. cordata (MEHC) in human lung carcinoma A549 cells. It was found that MEHC could inhibit the cell growth in a dose-dependent manner, which was associated with morphological change and apoptotic cell death as determined by formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase cells. Apoptosis of A549 cells by MEHC was also connected with a down-regulation of anti-apoptotic Bcl-2 and inhibitor of apoptosis proteins (IAPs) expression. MEHC treatment induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and a concomitant inhibition of poly(ADP-ribose) polymerase (PARP), ${\beta}$-catenin and phospholipase (PLC)-${\gamma}$1 protein expression. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.

• 농업과학연구
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• 제44권2호
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• pp.211-220
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• 2017

We conducted this study to determine the recommended application rate of fertilizer for Houttuynia cordata Thunb cultivation. The effects of various application levels of N, $P_2O_5$, and $K_2O$ fertilizers on the growth of this plant were investigated and the associated changes in soil properties were evaluated in the field. Soil pH at harvest time of Houttuynia cordata Thunb did not differ from that before fertilization, whereas EC tended to decrease during cultivation. The soil organic matter (SOM) and available phosphorus increased after treatment, and the amount of applied fertilizer ($P_2O_5$) and available phosphorus were proportional. The nitrogen absorption amount increased in N 100% treatment, but decreased in N 150% treatment. The phosphorus absorption amount rose with the fertilizer treatment concentration until $P_2O_5$ 150% treatment. The amount of absorbed potassium decreased in treatments with $K_2O$ 150% and $K_2O$ 200%. The plant length was the longest in N 100%, $P_2O_5$ 150%, and $K_2O$ 200%. The stem diameter was estimated to be 3.46 - 3.67 mm in N 100 - 200% treatment, 3.55 - 3.67 mm in $P_2O_5$ 100 - 150%, and 3.79 mm in $K_2O$ 200%. The number of tillers did not differ amongst fertilization treatments. The fresh weight was summed to be 3.67 ton/10 a in N 100% treatment, 3.79 Mg/10 a in $P_2O_5$ 150%, and 3.83 Mg/10 a in $K_2O$ 150%. Thus, the relationship between the fertilizer amount and yields of the plant showed that the most economical quantity of fertilizers should be 10.2 N kg/10 a, 5.5 $P_2O_5$ kg/10 a, and 8.2 $K_2O$ kg/10 a for Houttuynia Cordata Thunb.

• 한국식품과학회지
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• 제29권3호
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• pp.400-406
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• 1997

어성초의 화학조성과 항미생물 활성을 검색하였다. 어성초의 화학조성을 분석한 결과 유리아미노산은 Asp, Glu, Gly, Arg, Ala, Pro, Val, Cys, Phe 등이 함유되었고 유리당은 fructose, glucose, sucrose 및 maltose가 함유되어 있었다. 지방산 조성은 주로 linolenic acid, linoleic acid, oleic acid, palmitic acid로 구성되었으며 무기성분중에서는 K 함량이 특히 높은 것으로 분석되었다. 어성초 추출물을 용매분획하여 각 fraction에 대한 항미생물 활성을 검색한 결과 basic fraction을 제외한 acidic, neutral, phenolic fraction에서 항미생물 활성이 인정되었으며 neutral fraction>acidic fraction>phenolic fraction 순으로 높은 활성을 나타냈다. 사용 미생물중 Pseudomonas aeruginosa, Micrococcus luteus, Bacillus subtilis에 특히 높은 활성을 보였으며 Aspergillus parasiticus, Aspergillus flavus에도 비교적 높은 활성을 나타냈다. 활성이 인정된 각 fraction에 대한 MIC값은 neutral fraction의 경우 Pseud. aeruginosa가 0.0075 g/mL 상당량으로 가장 낮았으며 그 밖의 균주에 대하여는 $0.025{\sim}0.75g/mL$상당량의 다양한 MIC 값을 나타냈다. Acidic fraction과 phenolic fraction은 $0.25{\sim}0.75g/mL$ 상당량으로 neutral fraction에 비해 높은 MIC 값을 나타냈다. MLC값은 neutral fraction의 경우 Pseud. aeruginosa가 0.1g/mL 상당량으로 가장 낮았고 그밖의 균주에 대하여는 $0.25{\sim}2.0g/mL$ 상당량의 MLC 값을 보였으며 acidic fraction과 phenolic fraction의 경우 사용 미생물에 대하여 3.0g/mL상당량까지 치사활성을 보이지 않았다.

• 한국식품저장유통학회지
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• 제7권1호
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• pp.108-112
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• 2000

Proximate composition , volatile compounds, free amino acids, fatty acids and inorganic compounds in leaf, stem and root of Houttyunia cordata Thunb cultivated from two different area, Bosung and Sunchon , were analyzed. Each part of Houttuynia cordata Thunb from both local area showed moisture contents of 80-84% and crude ash contents of 2.1 ∼2.8%. Crude fat and protein contents were less than 3 % showing slightly higher contents in leaf than in both stem and root . Twenty six volatile compunds were identified from the parts of the plant, the volatile contents were high in the oder of leaf, root and stem. Major volatile compounds were mostly derivatives of decanoic acid ; decanoic acid, 20 tridecanoie, decanal and dodecanoic acid. Of free amino acids in leaf asparagine was the highest, while in stem hydroxyproline, proline and arginine were the major free amino acids. Linoleic acid was the highest in the stem and root, and linolenic acid was highest in leaf. the major minerals of all parts were K, Ca. Mg, P, Fe, Zn and Cu, showing highest with K.

• 한국응용과학기술학회지
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• 제38권1호
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• pp.217-227
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• 2021

본 연구는 국내에서 재배한 어성초를 각기 다른 조건에서 저장한 후 어성초의 추출액을 이용한 천연 화장품 소재로서의 타당성을 분석하였다. quercetin 의 함량은 건 어성초보다 생 어성초에서 더 높았으며, 총 폴리페놀 함량과 총 플라보노이드 함량을 측정한 결과 건 어성초가 생 어성초에 비해 상대적으로 높은 함량으로 분석되었으나 DPPH 및 ABTS 라디칼 소거 활성은 생 어성초가 상대적으로 높은 결과를 보였다. ROS 소거능과 산화질소 생성 억제율은 생 어성초에서 높은 억제율을 보였으나 100 ㎍/mL의 농도에서 건 어성초가 산화질소를 더 감소시키는 것으로 나타났다. 염증성 사이토카인의 감소를 측정한 결과 IL-1β는 건 어성초가 비교적 높은 감소 효과가 나타났고, IL-6와 TNF-α는 생 어성초에서 높은 감소 효과를 나타냈다. 이상의 결과로부터 생 어성초는 초기 염증 반응에 효과적이며 건 어성초는 염증이 만성 단계로 악화하는 것을 예방하는데 효과적인 것으로 예상된다.

• 동의생리병리학회지
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• 제20권3호
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• pp.690-696
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• 2006

Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is a traditional medicinal plant generally used in Oriental medicine therapy. In the present study, we investigated the effect of water extract of H. cordafa (WEHC) on the growth of human breast carcinoma MCF-7 cells. Exposure of MCF-7 cells to WEHC resulted in growth inhibition and apoptosis in a dose-dependent manner as measured by MTT assay and fluorescent microscope. The anti-proliferative effect of WEHC was associated with a dose-dependent up-regulation of cyclin-dependent kinase inhibitor p21 in a p53-independent fashion. We found WEHC decreased the levels of cyclooxygenase (COX)-2 and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1 , which was correlated with a decrease in prostaglandin $E_2{\;}(PGE_2)$ synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.121.1-121.1
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• 2003

Houttuynia cordata Thunb (HCT) is a herbal plant growing in China, Japan and Korea. According to the records of the Chinese medicine, this plant has been used as folk medicine for analgesics, beriberi, edema, hepatitis, icterus, etc. TCDD (2, 3, 7, 8 -tetrachlorodibenzo-p-dioxin), one of the notorious toxic environmental pollutants, damages various organs including liver and is regarded as an endocrine disrupter. After 7 days from TCDD (1$\mu$g/kg) injection, HCT (200 mg/kg) was administered into rats intraperitoneally for 4 weeks. (omitted)

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.208.2-208.2
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• 2003

TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin), one of the notorious toxic environmental pollutants, damages various organs including liver and is regarded as an endocrine disrupter. To investigate the effects of Houttuynia cordata Thunb (HCT) on the biochemical parameters of function, liver and serum of TCDD-treated rats were used. After 7 days from TCDD (1 $\mu\textrm{g}$/kg) injection, HCT (200 mg/kg) was administered into rats intraperitoneally for 4 weeks. The lipidperoxide content was examined by measuring the level of total cholesterol, HDL-cholesterol, LDL-cholesterol, total lipid and triglyceride (TG) in serum, and malondialdehyde (MDA) in liver tissue of rats. (omitted)

• 한국식품영양과학회지
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• 제27권6호
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• pp.1223-1229
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• 1998

This study was designed to observe the effects of feeding Houttuynia cordata juice and powder on the improvement of the lipids in the serum of dietary hypercholesterolemic rats. Experimental groups such as 5% cellulose for control group, 5%, 10%, 15%, 20% H. cordata powder for group 2, 3, 4, 5, respectively, and 5%, 10%, 15% H. cordata juice for group 6, 7, 8, respectively, were admi nistered to the male rats of the Sprague Dawley for 4 weeks. Concentrations of total cholesterol in serum were significantly lower in all experimental groups than in the control group, and particularly, the lowest in group 8. The ratio of HDL cholesterol to total cholesterol was the highest in group 5. Atherosclerotic index was lower in group 5 and 8. Concentrations of free cholesterol and cholesterol ester in serum were significantly lower in all experimental groups than in the control group, and then concentrations of free cholesterol and cholesterol ester were the lowest in group 5 and 8, re spectively. Concentrations of triglyceride and phospholipid were the lowest in the group 5. Con centrations of chylomicron and VLDL were significantly lower in all experimental groups than in the control group. And the concentration was remarkably the lowest in group 5. Concentration of glucose in blood was not significantly different, but lower in the group 5 and 8. Concentrations of Ca, P, Mg, Fe were not significantly different in all experimental groups. The GOT and GPT activites were lower in all experimental groups than in the control group, especially the lowest in group 7 of GOT and group 8 of GPT. The LDH activity was the lowest in group 8.