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Antiproliferative effect of Houttuynia cordata Thunb was Associated with the Inhibiton of Cyclooxygenase-2 Expression in Human Breast Carcinoma Cells  

Jung, Il-Hong (Department of Internal Medical, Dongeui University College of Oriental Medicine)
Jo, In-Joo (Department of Internal Medical, Dongeui University College of Oriental Medicine)
Park, Cheol (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine)
Park, Dong-Il (Department of Internal Medical, Dongeui University College of Oriental Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.20, no.3, 2006 , pp. 690-696 More about this Journal
Abstract
Houttuynia cordata Thunb, well known as 'E-Sung-Cho' in Korea, is a traditional medicinal plant generally used in Oriental medicine therapy. In the present study, we investigated the effect of water extract of H. cordafa (WEHC) on the growth of human breast carcinoma MCF-7 cells. Exposure of MCF-7 cells to WEHC resulted in growth inhibition and apoptosis in a dose-dependent manner as measured by MTT assay and fluorescent microscope. The anti-proliferative effect of WEHC was associated with a dose-dependent up-regulation of cyclin-dependent kinase inhibitor p21 in a p53-independent fashion. We found WEHC decreased the levels of cyclooxygenase (COX)-2 and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1 , which was correlated with a decrease in prostaglandin $E_2{\;}(PGE_2)$ synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of H. cordata.
Keywords
Houttuynia cordata Thunb; MCF-7; p2; COX-2; $PGE_2$;
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1 Beinfield, H. and Korngold, E. Chinese medicine and cancer care. Altern. Ther. Health Med. 9, 8-52, 2003
2 Monti, D.A. and Yang, J. Complementary medicine in chronic cancer care. Semin. Oncol. 32, 225-231, 2005   DOI   ScienceOn
3 Choi, Y.H., Kim, E.Y., Park, K.Y., Rhee, S.H. and Lee, W.H. Antimutagenic effects of the juice and boiling water extract of Houttuynia cordata Thunb. J. Kor. Soc. Food Sci. Nutr. 23, 916-921, 1994
4 Kim, J., Ryu, H.S., Shin, J.H. and Kim, H.S. In vitro and ex vivo supplementation of Houttuynia cordata extract and immunomodulating effect in mice. J. Kor. Soc. Food Sci. Nutr. 34, 167-175, 2005   과학기술학회마을   DOI
5 Lee, S.T., Lee, Y.H., Choi, Y.J., Shon, G.M., Lee, H.J. and Heo, J.S. Composition of quercetin and soluble tanin in Houttuynia cordata Thunb according to growth stages and plant parts. Kor. J. Medicinal Corp Sci. 10, 12-16, 2002
6 Toda, S. Antioxidative effects of polyphenols in leaves of Houttuynia cordata on protein fragmentation by copper-hydrogen peroxide in vitro. J. Med. Food. 8, 266-268, 2005   DOI   ScienceOn
7 Jeong, H.W., Choi, J.H. and Jin, C.S. Effects of water extracts of Flos Lonicerae and herba on the proliferation of human cancer cell lines. Kor. J. Oriental Med. Pathol. 10, 126-132, 1996
8 Bae, W.Y., Go, H.G. and Kim, C.H. Experimental study on the effects of heral acupuncture on the growth of melanoma B16 in mice. J. Kor. Acupuncture Moxibustion Soc. 18, 186-201, 2001
9 Chang, J.S., Chiang, L.C., Chen, C.C., Liu, L.T., Wang, K.C. and Lin, C.C. Antileukemic activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb. Am. J. Chin. Med. 29, 303-312, 2001   DOI   ScienceOn
10 Harper, J.W. Cyclin dependent kinase inhibitors. Cancer Surv. 29, 91-107, 1997
11 Tchou, W.W., Rom, W.N. and Tchou-Wong, K.M. Novel form of p21 (WAF1/CIP1/SDI1) protein in phorbol ester-induced G2/M arrest. J. Biol. Chem. 271, 29556-29560, 1996   DOI   ScienceOn
12 Thun, M.J., Henley, S.J. and Patrono, C. Nonsteroidal anti-inflammatory drugs as anticancer agents: mechanistic, pharmacologic, and clinical issues. J. Natl. Cancer Inst. 94, 252-266, 2002   DOI   ScienceOn
13 Surh, Y.J., Chun, K.S., Cha, H.H., Han, S.S., Keum, Y.S., Park, K.K. and Lee, S.S. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-$\kappa$B activation. Mutat. Res. 480-481, 243-268, 2001   DOI
14 Li, G.Z., Chai, O.H., Lee, M.S., Han, E.H., Kim, H.T. and Song, C.H. Inhibitory effects of Houttuynia cordata water extracts on anaphylactic reaction and mast cell activation. Biol. Pharm. Bull. 28, 1864-1868, 2005   DOI   ScienceOn
15 Yamamoto, Y. and Gaynor, R.B. Therapeutic potential of inhibition of the NF-$\kappa$B pathway in the treatment of inflammation and cancer. J. Clin. Invest. 107, 135-142, 2001   DOI   ScienceOn
16 Cha, J.Y., Jeon, B.S., Park, J.W., Moon, J.C. and Cho, Y.S. Effects of fermented compositions containing Inonotus obliquus and Houttuynia cordata on growth of human AGS gastric and HCT-15 colon cancer cells. J. Kor. Soc. Appl. Biol. Chem. 47, 202-207, 2004.
17 Song, J.H., Kim, M.J., Kwon, H.D. and Park, I.H., Antimicrobial activity of fractional exyracts from Houttuynia cordata root. J. Kor. Soc. Food Sci. Nutr. 32, 1053-1058, 2003   DOI
18 Sawaoka, H., Tsuji, S., Tsujii, M., Gunawan, E.S., Sasaki, Y., Kawano, S. and Hori, M. Cyclooxygenase inhibitors suppress angiogenesis and reduce tumor growth in vivo. Lab. Invest 79, 1469-1477, 1999
19 Giercksky, K.E. COX-2 inhibition and prevention of cancer. Best Pract. Res. Clin. Gastroenterol. 15, 821-833, 2001   DOI
20 Musgrove, E.A., Davison, E.A. and Ormandy, C.J. Role of the CDK Inhibitor p27 (Kip1) in mammary development and carcinogenesis: Insights from knockout mice. J. Mammary Gland Biol. Neoplasia 9, 55-66, 2004   DOI   ScienceOn
21 Kim, H.J. Biological effects of Houttuynia cordata Thunb extract on murine and human immuno system. MS Thesis, Seoul National University, 2000
22 Datto, M.B., Yu, Y. and Wang, X.F. Functional analysis of the transforming growth factor $\beta$ responsive elements in the WAF1/Cip1/p21 promoter. J. Biol. Chem. 270, 28623-28628, 1995   DOI
23 Wang, Y.S. Jungyi Yakli Yo Yngyong. People's Sanitation Publishing Co., Peking, p 709-710, 1983
24 Choi, Y.H. Proteasome-mediated degradation of BRCA1 protein in MCF-7 human breast cancer cells. Int. J. Oncol. 19, 687-693, 2001
25 Kim, S.K., Ryu, S.Y., No, J., Choi, S.U. and Kim, Y.S. Cytotoxic alkaloids from Houttuynia cordata. Arch. Pharm. Res. 24, 518-521, 2001   DOI
26 Vainio, H. Is COX-2 inhibition a panacea for cancer prevention? Int. J. Cancer 94, 613-614, 2001   DOI   ScienceOn
27 Dempke, W., Rie, C., Grothey, A. and Schmoll, H.J. Cyclooxygenase-2: a novel target for cancer chemotherapy? J. Cancer Res. Clin. Oncol. 127, 411-417, 2001   DOI   ScienceOn
28 Dulic, V., Stein, G.H., Far, D.F. and Reed, S.I. Nuclear accumulation of p21Cip1 at the onset of mitosis: a role at the G2/M-phase transition. Mol. Cell Biol. 18, 546-557, 1998   DOI
29 Chun, E.Y. Partial purification of Houttuynia cordata Thunb extract and characterization of its immunological activities in human. MS Thesis, Seoul National University, 1997
30 Choi, Y.H., Lee, W.H., Park, K.Y. and Zhang L. p53-independent induction of p21 (WAF1/CIP1), reduction of cyclin B1 and G2/M arrest by the isoflavone genistein in human prostate carcinoma cells. Jpn. J. Cancer Res. 91, 164-173, 2000   DOI
31 Zeng, Y.X. and El-Deiry, W.S. Regulation of p21WAF1/CIP1 expression by p53-independent pathways. Oncogene 12, 1557-1564, 1996
32 Xiong, Y., Hannon, G.J., Zhang, H., Casso, D., Kobayashi, R. and Beach, D. p21 is a universal inhibitor of cyclin kinases. Nature 366, 701-704, 1993   DOI   ScienceOn
33 Chung, C.K.,, Ham, S.S., Lee, S.Y., Oh, D.H., Choi, S.Y., Kang, I.J. and Nam, S.M., Effects of Houttuynia cordata ethanol extracts on serum lipies and antioxidant enzymes in rats fed high fat diet. J. Kor. Soc. Food Sci. Nutr. 28, 205-211, 1999
34 Yoo, H.T., Noh, J.S. and Lim, Y.D. Hyangyak Jipsungbang, Haenglim Publishing Co., Seoul, p 717-718, 1977