• Natural Product Sciences
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• v.12 no.4
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• pp.210-213
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• 2006

A 4,5-dioxoaporphine type alkaloid, cepharadione B (1), a phenolic acid, protocatechuic acid (2), and flavonols, quercetin (3), afzelin (4), and quercitrin (5), were isolated from the EtOAc-soluble extract of the whole plants of Houttuynia cordata. All the isolates (1-5) were subjected to in vitro bioassays to evaluate advanced glycation end products (AGEs) formation and rat lens aldose reductase (RLAR) inhibitory activity. The three flavonols 3-5 exhibited a significant inhibitory activity on AGEs formation with $IC_{50}$ values of 66.9, 58.9, and $32.3{\mu}M$, respectively. While the two flavonol rhamnosides 4 and 5 showed a remarkable inhibitory activity against RLAR with $IC_{50}$ values of 0.81 and $0.16{\mu}M$, respectively.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.529-536
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• 2007

The purpose of this study was to elucidate the effect of Oldenlandiae herba and Houttuyniae cordata on calf diarrhea in farms. We examined the antibacterial and antiviral effect of Oldenlandiae herba and Houttuyniae cordata extracts in vitro. And we divided diarrheal calves into three roups in farm; Oldenlandiae herba administration group(10 calves), Oldenlandiae herba and Houttuyniae cordata administration group(10 calves) and control group(7 calves). We estimated the therapeutic effect of the calf diarrhea by using clinical signs, CBC, AST, BUN, creatinine, and measurement of lymphocyte distribution in whole blood. Oldenlandiae herba and Houttuyniae cordata extracts by ethanol(98%) had antibacterial and antiviral effect. In the condition of diarrhea, the fecal condition of Oldenlandiae herba and Houttuyniae cordata group was relieved more than that of others group during experimental period. PCV and fibrinogen concentrations were high in control group. AST and BUN were within normal range in all groups. Administration of Oldenlandiae herba and Houttuyniae cordata was not toxic to the liver and kidney. And Oldenlandiae herba and Houttuyniae cordata extracts also affected the lymphocytes distribution in blood. From these results, we suggested that administration of Oldenlandiae herba and Houttuyniae cordata should be effective on the dairy calf diarrhea in farms.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.6
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• pp.1223-1229
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• 1998

This study was designed to observe the effects of feeding Houttuynia cordata juice and powder on the improvement of the lipids in the serum of dietary hypercholesterolemic rats. Experimental groups such as 5% cellulose for control group, 5%, 10%, 15%, 20% H. cordata powder for group 2, 3, 4, 5, respectively, and 5%, 10%, 15% H. cordata juice for group 6, 7, 8, respectively, were admi nistered to the male rats of the Sprague Dawley for 4 weeks. Concentrations of total cholesterol in serum were significantly lower in all experimental groups than in the control group, and particularly, the lowest in group 8. The ratio of HDL cholesterol to total cholesterol was the highest in group 5. Atherosclerotic index was lower in group 5 and 8. Concentrations of free cholesterol and cholesterol ester in serum were significantly lower in all experimental groups than in the control group, and then concentrations of free cholesterol and cholesterol ester were the lowest in group 5 and 8, re spectively. Concentrations of triglyceride and phospholipid were the lowest in the group 5. Con centrations of chylomicron and VLDL were significantly lower in all experimental groups than in the control group. And the concentration was remarkably the lowest in group 5. Concentration of glucose in blood was not significantly different, but lower in the group 5 and 8. Concentrations of Ca, P, Mg, Fe were not significantly different in all experimental groups. The GOT and GPT activites were lower in all experimental groups than in the control group, especially the lowest in group 7 of GOT and group 8 of GPT. The LDH activity was the lowest in group 8.

• Korean Journal of Pharmacognosy
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• v.31 no.2
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• pp.228-234
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• 2000

Effects of Houttuynia cordata on the level of lipid peroxide and the enzyme activities of the liver were investigated in bromobenzene-induced rats. Lipid peroxide content in liver was increased by bromobenzene. It was decreased when the methanol extract from the aerial parts of H. cordata was treated to the rat. The methanol extract reduced the activities of aminopyrine N-demethylase and aniline hydroxylase that increased by bromobenzene, however did not affect glutathione S-transferase activity. The methanol extract recovered the activity of epoxide hydrolase activity that decreased significantly by bromobenzene. We suggest that under our experimental conditions the extract might play an important play in the prevention of hepatotoxicity by reduction of aminopyrine N-demethylase and aniline hydroxylase activities as well as enhancement of epoxide hydrolase activity. Six phenolic compounds have been isolated from H. cordata and identified by means of spectral analysis as protocatechuic acid, quercetin, apigenin, afzelin, hyperoside and quercitrin.

• Research in Plant Disease
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• v.12 no.1
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• pp.55-57
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• 2006

Stem rot of Houttuynia cordata occurred in the experiment field of Research Institute of Natural Product, Uiseong, Gyeongbuk, Korea in July 2003. The typical symptoms of the disease were water-soaking, stem rot, necrotic tuber. White mycelial mats were spread over lesions and sclerotia were formed on died plant and near soil line. The sclerotia were spherical and irregular, 1-2.5 mm on potato dextrose agar (PDA), white to brown in color, The optimal temperature for hyphal growth was $28{\sim}30^{\circ}C$ and the hyphal diameter was $3{\sim}9{\mu}m$. Clamp connections were observed on PDA. Pathogenicity of the casual organism was provided on H. cordata according to Koch's postulate. The fungus was identified as Sclerotium rolfsii Saccardo on the basis of mycological characteristics and pathogenicity test to host plants. This is the first report on the stem rot of H. cordata caused by S. rolfsii in Korea.

• Korean Journal of Medicinal Crop Science
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• v.25 no.6
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• pp.404-410
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• 2017

Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells. Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at $500{\mu}g/m{\ell}$, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner. Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.

• Journal of Environmental Science International
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• v.26 no.12
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• pp.1355-1362
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• 2017

This study was performed to evaluate the possibility of application of lactic acid bacteria fermentation to increase the anti-allergic activity of the extracts from Houttuynia cordata Thunb. H. cordata Thunb was fermented on 25, 30, 35 and $40^{\circ}C$ for 5 days by two species of lactic acid bacteria, Leuconostoc mesenteroides 4395 and Lactobacillus sakei 383. The anti-allergic activity of water extracts of H. cordata Thunb was then analyzed both before and after fermentation. Anti-allergic activity was determined in vitro assays by using 5-lipoxygenase (5-LO), cyclooxygenase-2 (COX-2) and ${\beta}$-hexoseaminidase release of RBL-2H3 cells (degranulation marker). The extracts fermented at $35^{\circ}C$ by both bacteria had the highest inhibitory activities against 5-LO, and also higher than the control, and the one fermented at $30^{\circ}C$ by both bacteria had the highest inhibitory activity against COX-2. The degranulation of RBL-2H3 cells induced by IgE-antigen complex was estimated as ${\beta}$-hexoseaminidase release rate as reference of 100%, the release rates were inhibited in $25{\mu}g/ml$ of the extracts fermented at 30, 35 and $40^{\circ}C$ only by L. mesenteroides 4395. These results indicate that anti-allergic activity of H. cordata Thunb is increased by lactic acid bacteria fermentation.

• Korean Journal of Agricultural Science
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• v.44 no.2
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• pp.211-220
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• 2017

We conducted this study to determine the recommended application rate of fertilizer for Houttuynia cordata Thunb cultivation. The effects of various application levels of N, $P_2O_5$, and $K_2O$ fertilizers on the growth of this plant were investigated and the associated changes in soil properties were evaluated in the field. Soil pH at harvest time of Houttuynia cordata Thunb did not differ from that before fertilization, whereas EC tended to decrease during cultivation. The soil organic matter (SOM) and available phosphorus increased after treatment, and the amount of applied fertilizer ($P_2O_5$) and available phosphorus were proportional. The nitrogen absorption amount increased in N 100% treatment, but decreased in N 150% treatment. The phosphorus absorption amount rose with the fertilizer treatment concentration until $P_2O_5$ 150% treatment. The amount of absorbed potassium decreased in treatments with $K_2O$ 150% and $K_2O$ 200%. The plant length was the longest in N 100%, $P_2O_5$ 150%, and $K_2O$ 200%. The stem diameter was estimated to be 3.46 - 3.67 mm in N 100 - 200% treatment, 3.55 - 3.67 mm in $P_2O_5$ 100 - 150%, and 3.79 mm in $K_2O$ 200%. The number of tillers did not differ amongst fertilization treatments. The fresh weight was summed to be 3.67 ton/10 a in N 100% treatment, 3.79 Mg/10 a in $P_2O_5$ 150%, and 3.83 Mg/10 a in $K_2O$ 150%. Thus, the relationship between the fertilizer amount and yields of the plant showed that the most economical quantity of fertilizers should be 10.2 N kg/10 a, 5.5 $P_2O_5$ kg/10 a, and 8.2 $K_2O$ kg/10 a for Houttuynia Cordata Thunb.

• Korean Journal of Food Science and Technology
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• v.29 no.3
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• pp.400-406
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• 1997

Chemical composition and antimicrobial activity of Houttuynia cordata Thunb. were investigated to develop a natural food preservative from it. Aspartic acid, glutamic acid, glycine and arginine were major amino acids of Houttuynia cordata Thunb., but were present in a trace amount. Free sugars were composed of glucose, fructose, sucrose and maltose and major fatty acids were linolenic acid, linoleic acid, oleic acid, and palmitic acid. Mineral elements were potassium, calcium, magnesium, iron, zinc, and copper. Antimicrobial activities were shown in acidic, neutral and phenolic fraction of Houttuynia cordata Thunb., but not in basic fraction. Among the four fractions, neutral fraction showed the strongest antimicrobial activities against microorganisms tested, such as Pseudomnas aeruginosa, Micrococcus luteus, Bacillus subtilis, Aspergillus parasiticus and Aspergillus flavus. Minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) of the neutral fraction varied according to microoganisms tested. The lowest values of MIC (0.0075 g eq./mL) and MLC (0.10 g eq./mL) were obtained from Pseudomonas aeruginosa.

• Journal of Life Science
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• v.29 no.6
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• pp.705-711
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• 2019

Benign prostatic hyperplasia (BPH) is the most common urogenital disorder in men, benign tumor and is a typical disease deteriorating the quality of old men's lives, and its prevalence increases with age. Though the molecular pathogenesis of BPH has not yet been clearly revealed, it is known that the variation and aging of the endocrine including sex hormone may cause BPH. Especially the hypertrophy of the prostate cell by the formation of the excessive dihydrotestosterone (DHT) is estimated to cause BPH. If testosterone exists excessively in blood, a lot of DHT is produced in prostate by $5{\alpha}-reductase$. Thus, in this study we tried to analyze haematological change and histopathological change by using the model rat with BPH caused by hypodermic injection of testosterone to prove the effect of Houttuynia cordata extracts on BPH. Rats were divided into four experimental groups: no treatment group (N), the testosterone injection and D.W treatment group (DO), the testosterone injection and Houttuynia cordata treatment group (HO) and testosterone injection and finasteride treatment group (FO). Prostate weight, volume and weight ratio in the HO and FO groups were significantly lower than the DO group. Testosterone and DHT levels in the HO group were significantly lower than the DO group. The HO and FO groups showed trophic symptoms and were lined by flattened epithelial cells, thus, the stromal proliferation is relatively low as compared to the DO group. These results suggest that Houttuynia cordata may control benign prostatic hyperplasia.