• Natural Product Sciences
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• v.9 no.2
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• pp.97-101
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• 2003

This study was undertaken to evaluate the antinociceptive and antiinflammatory effects of the heartwood extract of Rhus verniciflua (Anacardiaceae) and the two major components, sulfuretin and fustin. The MeOH extract, its EtOAc-soluble portion and sulfuretin showed significant antinociceptive activity in writhing and hot plate test assays and antiinflammaory effects in carrageenan-induced hind paw edema in rats. In particular, treatment of sulfuretin with 10 mg/kg dose (i.p.) reduced writhing frequency by 48.0% (p<0.01) compared to that of a control group. Further, the treatment of sulfuretin (5, 10 mg/kg, i.p.) for 7 days prevented the carrageenan-induced hind paw edema significantly (p<0.01). The antiinflammatory effect of sulfuretin was also confirmed by microscopic observation of mast cell numbers in muscle. In addition, sulfuretin suppressed the cyclooxygenase- 2 (COX-2) activity $(IC_{50}\;=\;28.7\;{\mu}M)$ in lipopolysaccharide-activated macrophage cells. This result indicates that the inhibitory effect of sulfuretin on COX-2 may be one of the antinociceptive/antiinflammatory mechanism.

• Proceedings of the Microbiological Society of Korea Conference
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• 2008.05a
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• pp.135-135
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• 2008

Various biosensors were evaluated for identifying and detecting foodborne pathogens in a rapid and effective manner. First, five strains of Escherichia coli and six strains of Salmonella were identified using Fourier transform infrared spectroscopy and a statistical program. For doing this, lipopolysaccharides (LPSs) and outer membrane proteins (OMPs) were extracted from a cell wall of each bacterial strain. As a result, each strain was identifed at the level of 97% for E. coli and 100% for Salmonella. Second, E. coli O157:H7, S. Enteritidis, and Listeria monocytogenes were identified by multiplex PCR products from four specific genes of each bacteria using a capillary electrophoresis (CE). Also, ground beef for E. coli O157:H7, lettuce for S. Enteritidis, and hot dog for L. monocytogenes were used to determine the possibility of detecting pathogens in foods. Foods inoculated with respective pathogen were cultivated for six hours and multiplex PCR products were obtained and assessed. The minimum detection levels of tested bacteria were <10 cells/g, <10 cells/g, and $10^4$ cells/g for E. coli O157:H7, S. Enteritidis, and L. monocytogenes, respectively. Third, it was possible to detect S. Typhimurium in a pure culture and lettuce by a bioluminescence-based detection assay using both recombinant bacteriophage P22::luxI and a bioluminescent bioreporter. In addition, bacteriophage T4 was quantitatively monitored using E. coli including luxCDABE genes.

• Mycobiology
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• v.34 no.3
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• pp.138-142
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• 2006

Soil cyanobacteria isolated from the rice paddy fields of 10 different locations across Korea were evaluated by agar plate diffusion test for antifungal activity. Aqueous, petroleum ether, and methanol extracts from one hundred and forty two cyanobacterial strains belonging to the 14 genera were examined for antifungal properties against seven phytopathogenic fungi causing diseases in hot pepper (Capsicum annuum L). Of total cyanobacteria, nine cyanobacteria (6.34%) exhibited antifungal effects. The nine cyanobacteria selected with positive antifungal activities were two species of Oscillatoria, two of Anabaena, three of Nostoc, one of Nodularia, and one of Calothrix. Alternaria alternata and Botrytis cinerea were inhibited by nine and eight species of cyanobacteria, respectively. Rhizopus stolonifer was suppressed by only methanol extract of Nostoc commune FK-103. In particular, Nostoc commune FK-103 and Oscillatoria tenuis FK-109 showed strong antifungal activities against Phytophthora capsici. Their antifungal activity at the late exponential growth phase is related to the growth temperature and not associated with the growth parameters such as cell biomass and $chlorophyll-{\alpha}$ concentration. The high inhibition levels of antibiotics were 22.5 and 31.8 mm for N. commune FK-103 and O. tenuis FK-109, respectively. The optimal temperature for antibiotic productivity was $35^{\circ}C$.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.431-437
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• 2003

The graphic simulator has been used to analyze the problems that can occur during transporting and handling radioactive materials, and to derive necessary devices that can transport and handle spent fuel powder without scattering in a hot cell. The graphic simulator has advantages over the real scale physical mockup with respect to cost and schedule. The process equipment and maintenance devices can be verified in advance with less cost and reduced schedule. The derived results are being reflected in the design of equipment for demonstration and are being verified during demonstration.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.596-600
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• 2003

The Advanced spent fuel Conditioning Process(ACP) is under development for the effective management of spent fuel which had been generated in nuclear plants. The ACP needs a hot cell where most operations will be peformed. To give priority to the environment safety, radiation doses evaluation for the radioactive nuclides were preliminarily peformed in both normal operation and accident case. The evaluation result shows a safe margin for regulation limits and SAR limit of IMEF where this facility will be constructed.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.621-626
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• 2003

A DUPIC nuclear fuel is a newly developed fuel for CANDU reactors based on the concept of refabrication of spent PWR fuel by a dry process. Because a spent PWR fuel, a highly radioactive material, is used as a starting material, the experimental verification of DUPIC nuclear fuel fabrication requires an appropriate facility which should satisfy engineering requirements and guarantees safe operation. DUPIC nuclear fuel development team modified M6 hot-cell in IMEF to construct the dedicated facility(DFDF) for tile experiment. The experiment with spent PWR fuel have been conducted since January of 2000. Environmental effects of DFDF normal operation have been investigated when DUPIC nuclear fuel is fabricated with the maximum capacity of 50kg U/yr. The analysis results of the radiological safety of DFDF facility have shown that both national regulation limit and IMEF design criteria are satisfied.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.11
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• pp.5264-5281
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• 2017

Multihop relay-based cellular networks are attracting much interest because of their throughput enhancement, coverage extension, and low infrastructure cost. In these networks, relay stations (RSs) between a base station (BS) and mobile stations (MSs) drastically increase the overall spectral efficiency, with improved channel quality for MSs located at the cell edge or in shadow areas, and enhanced throughput of MSs in hot spots. These relay-based networks require an advanced radio resource management scheme because the optimal amount of radio resource for a BS-to-RS link should be allocated according to the MS channel quality and distribution, considering the interference among RSs and neighbor BSs. In this paper, we propose optimal resource planning algorithms that maximize the overall utility of relay-based networks under a proportional fair scheduling policy. In the first phase, we determine an optimal scheduling policy for distributing BS-to-RS link resources to RSs. In the second phase, we determine the optimal amount of the BS-to-RS link resources using the results of the first phase. The proposed algorithms efficiently calculate the optimal amount of resource without exhaustive searches, and their accuracy is verified by comparison with simulation results, in which the algorithms show a perfect match with simulations.

• Journal of Animal Environmental Science
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• v.20 no.4
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• pp.147-154
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• 2014

Heat stress is a significant burden to animal production in most areas of the world. Improving our knowledge of physiological and metabolic mechanisms of acclimation may contribute to the development of procedures that may help to maintain health and production efficiency under hot temperature. The effect of Ulmus pumila (UP) extract in inducing Heat Shock Proteins (HSPs) expression in heat-stressed RAW264.7 macrophage cells was investigated. Cell viability assay showed a dose dependent increase in cells after treatment with UP for 24 hours. RT-PCR and western blot analysis showed that increasing concentrations of UP induce the expression of Heat Shock Factor 1 (HSF1) and dose dependently upregulated the expression of Heat shock protein 70 (Hsp70) and Hsp90. LPS-induced nitric oxide was dose-dependently reduced while phagocytic activity greatly recovered with UP treatment. These data demonstrated that UP can be a potential candidate in the development of cytoprotective agent against heat stress.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.1
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• pp.32-37
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• 2006

In this study, we have purified and characterized the membrane bound nitrate reductase obtained from the denitrifying bacteria, Ochrobactrum anthropi SY509, which was isolated from soil samples. O. anthropi SY509 can grow in minimal medium using nitrate as a nitrogen source. We achieved an overall purification rate of 15-fold from the protein extracted from the membrane fraction, with a recovery of approximately 12% of activity. The enzyme exhibited its highest level of activity at pH 5.5, and the activity was increased up to $70^{\circ}C$. Periplasmic and cytochromic proteins, including nitrite and nitrous oxide reductase, were excluded during centrifugation and were verified using enzyme essay. Reduced methyl viologen was determined to be the most efficient electron donor among a variety of anionic and cationic dyestuffs, which could be also used as an electron donor with dimethyl dithionite. The effects of purification and storage conditions on the stability of enzyme were also investigated. The activity of the membranebound nitrate reductase was stably maintained for over 2 weeks in solution. To maintain the stability of enzyme, the cell was disrupted using sonication at low temperatures, and enzyme was extracted by hot water without any surfactant. The purified enzyme was stored in solution with no salt to prevent any significant losses in activity levels.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.05a
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• pp.680-686
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• 1995

It has been known that water-side corrosion of fuel rods in nuclear reactor is accompanied with the loss of metallic wall thickness and pickup of hydrogen. This corrosion is one of the important limiting factors ill the operating life of fuel rods. In connection with the fuel cladding corrosion, a device to measure the water-side oxide layer thickness by means of the eddy-current method without destructing the fuel rod was developed by KAERI. The device was installed on the multi-function testing bench in the nondestructive test hot-cell and its calibration was carried out successfully for the standard rod attached with plastic thin films whose thicknesses are predetermined. It shows good precision within about 10% error. And a PWR fuel rod, one of the J-44 assembly discharged from Kori nuclear power plant Unit-2, has been selected for oxide layer thickness measurements. With the result of data analysis, it appeared that the oxide layer thicknesses of Zircaloy cladding vary with the length of the fuel rod, and their thicknesses were compared with those of the destructive test results to confirm the real thicknesses.