• 식물병연구
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• 제6권1호
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• pp.27-33
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• 2000

심하게 이병된 배추포장내 무사마귀병균의 수직분포를 보면 토양의 심도가 깊어질수록 무사마귀병균의 밀도는 급격히 감소하였으며 무사마귀병균의 97%가 지하 5cm 이내의 표토에 분포하였고 지하 40cm 토양에서도 소량 검출되었다. 수평분포를 보면 무사마귀병균은 배추포기중심에 모여 있다기보다는 전 포장에 골고루 분포하고 있어서 한곳에 몰려 분포하는 현상은 발견되지 않았다. 평창의 23개 배추지역의 무사마귀병균의 밀도는 1$\times$$10^4$포자/g 토양 이하의 밀도가 낮은 곳에서부터 $10^{6}$ 토양이상의 밀도가 높은 포장까지 폭넓게 분포하고 있었다. 작물을 재배하기 않은 처녀지 토양은 밀도가 $10^4$토양 이하로 극히 낮거나 전혀 검출되지 않았으며 심하게 이병된 배추밭의 밀도는 $10^{5}$/g 토양이상으로 대단히 높았다. 건전 배추밭과 배추 이외의 타작물을 재배하고 있는 포장내 밀도는 이보다 상당히 낮아서 심고 있는 작물에 따라서 토양내 무사마귀병균 밀도에 큰 차이가 있었다 작부체계별 밀도분포를 보면 다소 예외는 있으나 배추, 무 등의 기주작물을 연작한 곳일수록 밀도가 높게 나타났으며, 약초, 옥수수, 메밀, 기타 채소 등 비기주작물을 재배한 곳일수록 무사마귀병균의 밀도가 낮은 곳이 많았다. 배추, 무 등의 기주작물을 연작하면 토양내 무사마귀병균의 밀도는 점차 상승하는 경향이었고 반면에 당귀, 메밀 등 비기주작물로 윤작한 포장의 밀도는 급격히 감소하는 경향이어서 무사마귀병균의 토양내 생존은 작부체계에 의해 크게 영향을 받고 있는 것으로 나타났다. 본 연구에서 사용한 개량형광염색방법은 기존의 방법에 비해 토양내 휴면포자의 식별이 보다 용이해졌을 뿐만 아니라 그 검출효율도 증진되었고 조작이 간편하여 큰 숙련없이 밀도를 측정할 수 있어 향후 토양내 무사마귀병균의 동태를 연구하는데 유용하게 이용될 수 있을 것으로 생각된다.다.

• 한국응용곤충학회지
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• 제13권4호
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• pp.227-231
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• 1974

본 시험은 우리나라 씨감자 주요 생산지인 대관령지방에 분포되어 있는 역병군의 생리형을 조사 판별하기 위해 71-73년에 걸쳐 수행되었던 바. 1. 1971년에는 68계통을 분리 접증 했는데 Race 0, Race 1, Race 2, Race 3, Race 1.3, Race 1.4, Race 3.4, Race 1.3.4, Race 2.3.4, Race 1.2.3.4등 10가지 생리형이 간별 되었으며 그중 Race 0와 Race 3가 제일 널리 분포 되었었고 2. 1972년에는 62계통을 분리 하였던 바 Race 0, Race 1, Race 4, Race 1.3, Race 1.3.4등 5가지 생리형이 나타났으며 그중 전해에 이어 Race 0가 널리 분포되어 있었다. 3. 1973년에는 76계통비서 Race 0, Race 3, Race4, Race 1.3, Race 3.4 Race 1.3.4 등 9가지 생리형이 나타났던 바 Race 0와 Race 4가 가장 널리 분포 되어있는 생리형으로 나타났다. 4. 이로써 우리나라에는 Race 0, Race 1, Race 2, Race 3, Race 4, Race 1.3, Race 1.4, Race 3.4, Race 1.3.4, Race 2.3. 4,Race 1.2.3.4 등 11가지 Race가 존재함이 밝혀졌으며 Race 0, Race 3과 Race 4가 가장 널리 분포 되어있는 생리형으로 나타났다. 5. 이와 같이 생리형은 매년 기후조건의 차이에 따라 발생정도가 다름을 알 수 있다.

• 한국균학회지
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• 제38권1호
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• pp.62-68
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• 2010

국내 참나무시들음병 병원균인 Raffaelea quercus-mongolicae에 대한 버섯 균주들의 길항 특성을 대치배양을 통해 조사하였다. 총 158개의 시험 균주 중 33개의 균주는 병원균에 대해 단기간 (6~7일) 및 장기간(27~31일) 길항력이 전혀 없었으며, 96개의 균주는 길항력이 미약하거나 길항력이 장기적으로 지속되지 못했다. 이와 달리 29개의 균주는 단기간 및 장기간 길항력이 각각 $\geq$ R+와 $\geq$ R로 타 시험 균주에 비해 높게 나타났으며, 이들과 같이 R. quercus-mongolicae에 대해 길항력을 갖는 균주들은 활엽수(참나무류 포함)나 침엽수 등 발생 기주가 서로 다른 다양한 서식지에서 살아가는 버섯 균종들의 균주임을 알 수 있었다. 참나무시들음병 피해목을 버섯 재배용 자원으로 활용하기 위해 이에 적합한 7개의 길항 균주를 다음과 같이 선발하였다; 붉은덕다리버섯(KFRI 914), 치마버섯(KFRI 832), 구름버섯(KFRI 100), 덕다리버섯(KFRI 654), 간버섯(KFRI 588), 표고(KFRI 269, 764).

• The Plant Pathology Journal
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• 제18권4호
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• pp.225-227
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• 2002

Moth orchid plants with yellowing blight and root rot symptoms were collected, and a total of 54 isolates of Fusarium spp. was obtained from roots and leaf bases of the diseased plants. The isolates were identified based on their morphological characteristics. Out of the 54 isolates of Fusarium spp., 42 isolates were identified as F. solani, 5 isolates as F. oxysporum, and 7 as F. proliferatum. Isolates of the three Fusarium spp. were tested for pathogenicity to moth orchid plants by artificial inoculation. All the Fusarium spp. induced root rot of the host plants. The symptoms progressed up to the basal part of the leaves, which later caused yellowing blight. The symptoms induced on the plants by artificial inoculation with the Fusarium spp. isolates were similar to those observed in greenhouses. The present study reveals that F. oxysporum, F. proliferatum, and F. solani cause root rot of moth orchid, and that F. solani is the main pathogen of the disease.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.113.2-114
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• 2003

Five pepper-infecting potyviruses, Pepper mottle virus (PepMoV), Chilli veinal mottle virus (CVMV), Pepper veinal mottle virus (PVMV), Pepper severe mosaic virus (PSMV) and Tobacco each virus (TEV), are known filamentous virus and can be infected pepper crops systemically. To understand pathology and genome information of the five viruses on pepper plants, host reactions and sequences were compared to the 5 viruses. Five potyviruses were inoculated onto some typical cultivars of hot peppers and compared their symptoms, and virus accumulations. A set of degenerate primers for potyviruses were applied to 5 viruses and RT-PCR was performed. RT-PCR products containing partial nuclear inclusion b and coat protein (CP) genes were cloned. Then, oligo dT primer and species-specific primer were redesigned to amplify the C-terminal part of CP and 3' noncoding regions of each viruses. Sequences of the viruses were analyzed and compared to serological relationships among the viruses. The data can be useful for screening of potyviruses in pepper plants and pathogen-derived transgenic pepper plant development.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.29-29
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• 2003

Incompatible plant-pathogen interactions result in the rapid cell death response known as hypersensitive response (HR) and activation of host defense-related genes. To understand the molecular and cellular mechanism controlling defense response better, several approaches including isolation and characterization of novel genes, promoter analysis of those genes, protein-protein interaction analysis and reverse genetic approach etc. By using the yeast two-hybrid system a clone named Tsipl, Tsil -interacting protein 1, was isolated whose translation product apparently interacted with Tsil, an EREBP/AP2 type DNA binding protein. RNA gel blot analysis showed that the expression of Tsipl was increased by treatment with NaCl, ethylene, salicylic acid, or gibberellic acid. Transient expression analysis using a Tsipl::smGFP fusion gene in Arabidopsis protoplasts indicated that the Tsipl protein was targeted to the outer surface of chloroplasts. The targeted Tsipl::smGFP proteins were diffused to the cytoplasm of protoplasts in the presence of salicylic acid (SA) The PEG-mediated co-transfection analysis showed that Tsipl could interact with Tsil in the nucleus. These results suggest that Tsipl-Tsil interaction might serve to regulate defense-related gene expression. Basically the useful promoters are valuable tools for effective control of gene expression related to various developmental and environmental condition.(중략)

• Parasites, Hosts and Diseases
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• 제54권3호
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• pp.339-344
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• 2016

The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.

• 한국토양비료학회지
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• 제51권1호
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• pp.28-34
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• 2018

Yuzu (Citrus junos) gummosis disease, caused by Phytophthora nicotianae, was first reported in 1997. As known in citrus, Phytophthora is the most fastidious soil-borne pathogen to control. In order to minimize its damage to Citrus spp., integrated pest management (IPM) approach, including fungicide chemicals and resistant cultivars, is necessary. Therefore, in this study we tried to evaluate tolerance of yuzu cultivars and its related species against yuzu Phytophthora. Trifoliate orange was evaluated as a susceptible host to yuzu Phytophthora by both mycelial growth onto extract media and immature fruit inoculation. However, in zoospores spray-inoculation on 2-year-old cuttings tree, trifoliate orange appeared to have a resistant property as showing less than 6% diseased leaf rate. Among yuzu cultivars only 'Namhae No. 1' appeared resistant property against both P. nicotianae and P. citrophthora. The 'Namhae No. 1' showed 5.7% and 10.6% diseased leaf ratio by P. nicotianae and P. citrophthora, respectively. Clearly, in order to reduce damages caused by two yuzu Phytophthora, we suggest that growers may utilize a trifoliate orange as a rootstock and 'Namhae No. 1' as a scion for fruit production.

• 한국미생물·생명공학회지
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• 제27권1호
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• pp.70-74
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• 1999

Vibrio vulnificus, a normal bacterial inhabitant of estuaries, is of concern because it can be a potent human pathogen, a causing septicemia, wound infections and gastrointestinal disease in susceptible host. In this survey, total 431 samples were obtained from different sites of the Chonman coastal area during the periods from Mar. 1997 to Feb. 1998. Vibrio vulnificus was isolated from the middle of May to the begining of November of 1997 in Chonman coastal area, as the seawater temperature was at 20$^{\circ}C$ and 15$^{\circ}C$, respectively and was rapidly increased to above 40% from July to September. The isolation rates of V. vulnificus from sediment, seawater, raw seafoods and aquarium water were 52.1%, 49.1%, 32.5%, and 27.3%, respectively and isolation rate was highest in oyster among various collected samples. V.vulnificus was also isolated from 73.1%(38/52) of sampling sites of Chonman coastal area.

• Clinical and Experimental Pediatrics
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• 제48권11호
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• pp.1153-1161
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• 2005

As known by other name(natural immunity), the innate immune system comprises all those mechanisms for dealing with infection that are constitutive or built in, changing little with age or with experience of infection. Though in some ways less sophisticated than adaptive immunity, innate immunity should not belittled, since it has evidently protected thousands of species of invertebrates sufficiently to survive for up to 2 billion years. In the innate immune system, molecules of both cellular and humoral types are involved, corresponding to the need to recognize and dispose of different types of pathogen, to promote inflammatory responses and to interact to the adaptive immune system. A major features of innate immunity are the presence of the normal gut flora, complements, macrophages, dendritic cells, natural killer cells and many cytokines that can block the establishment of infection. Both phagocytic cells and complement system have tremendous potential for damaging host cells, but fortunately they are normally only triggered by foreign materials, and usually most of their destructive effects are focussed on the surface of these or in the safe environment of the phagolysosome. This article addreses the comprehensive mechanisms of the major components of the innate immune system to prevent the infection.