• Journal of Microbiology and Biotechnology
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• 제29권12호
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• pp.1931-1937
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• 2019

The heterologous expression of the Streptomyces natural product (NP) biosynthetic gene cluster (BGC) has become an attractive strategy for the activation, titer improvement, and refactoring of valuable and cryptic NP BGCs. Previously, a Streptomyces artificial chromosomal vector system, pSBAC, was applied successfully to the precise cloning of large-sized polyketide BGCs, including immunosuppressant tautomycetin and antibiotic pikromycin, which led to stable and comparable production in several heterologous hosts. To further validate the pSBAC system as a generally applicable heterologous expression system, the daptomycin BGC of S. roseosporus was cloned and expressed heterologously in a model Streptomyces cell factory. A 65-kb daptomycin BGC, which belongs to a non-ribosomal polypeptide synthetase (NRPS) family, was cloned precisely into the pSBAC which resulted in 28.9 mg/l of daptomycin and its derivatives in S. coelicolor M511(a daptomycin non-producing heterologous host). These results suggest that a pSBAC-driven heterologous expression strategy is an ideal approach for producing low and inconsistent Streptomyces NRPS-family NPs, such as daptomycin, which are produced low and inconsistent in native host.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2002년도 추계학술대회 및 정기총회
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• pp.243-248
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• 2002

빠르게 변해 가는 정보화사회에서 침입 탐지 시스템은 정밀성과 적웅성, 그리고 확장성을 필요로 한다. 또한 복잡한 Network 환경에서 중요하고 기밀성이 유지되어야 할 리소스를 보호하기 위해, 더욱 구조적이고 지능적인 IDS(Intrusion Detection System)개발의 필요성이 요구되고 있다. 본 연구는 이를 위한, 지능적인 IDS를 위해 침입패턴을 생성하기 위한 모델을 도출함에 목적이 있다. 침입 패턴은 방대한 양의 데이터를 갖게 되고, 이를 정확하고 효율적으로 관리하기 위해서 데이터마이닝의 주요 2분야인 Link analysis와 Sequence analysis를 이용하여 정확하고 신뢰성 있는 침입규칙을 생성하기 위한 모델을 도출해낸다 이 모델은 "Time Based Traffic Model", "Host Based Traffic Model", "Content Model"로 각각 상이한 침입 패턴을 생성하게 된다. 이 모델을 이용하면 좀더 효율적이고 안정적으로 패턴을 생성 할 수 있다, 즉 지능형 시스템기반의 침입 탐지 모델을 구현할 수 있다. 이러한 모델로 생성한 규칙은 침입데이터를 대표하는 규칙이 되고, 이는 비정상 사용자와 정상 사용자를 분류하게 된다 모델에 사용된 데이터는 KDD컨테스트의 데이터를 이용하였다. 사용된 데이터는 KDD컨테스트의 데이터를 이용하였다.

• 정보통신설비학회논문지
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• 제1권1호
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• pp.51-64
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• 2002

This paper analyzes performance measures of a Bluetooth_based mobile home network system. The home network system consists of terminals with Bluetooth interfaces, access points (AP), a home PC, and a gateway A mobile host in wireless terminals uses Mobile IP for supporting the mobility This paper considers four types of data traffic, which are new connection traffic, handoff traffic, Internet data traffic, and control data traffic and suggests a queueing system model of the home network system, where the AP and the home PC are modeled as M/G/1 with four priority queues and the gateway is modeled as M/G/1 with a single queue The generation rate and service time of individual traffic influence their performance measures. Based ell the suggested model, we propose the elapsed time of data traffic in terms of the number of cells, the number of Home PCs, arrival rates of four types of traffic and the service rates of AP/Home PCs/Gateway To analyze influences on the elapsed time with respect to arrival rate of four types of traffic, some examples are given.

• 천문학회보
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• 제41권2호
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• pp.53.3-54
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• 2016

We combine a semi-analytic model of galaxy evolution with constraints on circumstellar habitable zones and the distribution of terrestrial planets in order to probe the suitability of galaxies of different mass and type to host habitable planets, and how it evolves with time. We find that the fraction of stars with terrestrial planets in their habitable zone (known as habitability) depends only weakly on galaxy mass, with a maximum around $4{\times}10^{10}M_{\odot}$. We estimate that 0.7% of all stars in Milky Way-type galaxies to host a terrestrial planet within their habitable zone, consistent with the value derived from Kepler observations. On the other hand, the habitability of passive galaxies is slightly but systematically higher, unless we assume an unrealistically high sensitivity of planets to supernovae. We find that the overall habitability of galaxies has not changed significantly in the last ~8 Gyr, with most of the habitable planets in local disk galaxies having formed ~1.5 Gyr before our own solar system. Finally, we expect that ${\sim}1.4{\times}10^9$ planets similar to present-day Earth have existed so far in our galaxy.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2005년도 춘계학술대회논문집
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• pp.188-191
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• 2005

Taguchi method is used to determine the optimal configuration of PZT (Lead Zirconate-Titanate) patch on the host structure for improving the performance of piezoelectric shunt system. The charges generated on the surface of PZT patch are selected to be the objective function in the Taguchi method. Full three dimensional finite element models are used to simulate vibration of smart panel and to obtain the admittance of the piezoelectric shunt system. Using Taguchi method in Minitab, the optimal model is obtained. The experiment with piezoelectric shunt circuit is performed to verify the validity of the optimal model comparing with initial model.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1998년도 The 12th Symposium on Plant Biotechnology Vol.12
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• pp.10-18
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• 1998

For improvement of plants in disease resistance, it is most important to elucidate the mechanism to perceive and respond to the signal molecules of invaders. A model system with pea and its pathogen, Mycosphaerella pinodes, showed that the fungal elicitor induced defense responses in all plant species tested but that the suppressor of the fungus blocked or delayed the expression of defense responses and induced accessibility only in the host plant. In the world, many researchers believe that the pathogens` signals are recognized only on the receptors in the plasma membranes. Though we found that the ATPase and polyphosphoinositide metabolism in isolated plasma membranes responded to these fungal signals, we failed to detect specific actions of the suppressor in vitro on these plasma membrane functions. Recently, we found that ATPase (NTPases) and superoxide generating system in isolated cell wall were regulated by these fungal signals even in vitro, especially, by the suppressor in a strictly species-specific manner and also that the cell wall alone prepared an original defense system. The effects of both fungal signals on the isolated cell wall functions in vitro coincide perfectly with those on defense responses in vivo. In this treatise, we discuss the key role of the cell wall, which is plant-specific and the most exterior organelle, in determining host-parasite specificity and molecular target for improvement of plants.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.40-50
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• 2002

Fruit fly, Drosophila melanogaster has developed efficient immune mechanisms to prevent microbial infection, which are consisted of cellular and humoral responses. During the systemic or local infection, two distinct pathways (Toll and Imd) play major roles in antimicrobial peptide synthesis. The Toll pathway is required to defend Gram-positive bacterial and fungal infections, whereas the Imd pathway is important in Gram-negative bacterial infection. We have shown that the infection of the opportunistic Gram-negative bacterium, Pseudomonas aeruginosa strain PA14 (PA14) into fly dorsal thorax can kill the flies within 48 h ($100\%$ mortality) in our optimized infection condition, suggesting that the PA14 strain can cause disease progress in fly model system. We found that flies carrying a constitutively activated mutant form of the Toll receptor $(Tl^{10b})$ showed increased resistance to P. aeruginosa infection and that flies carrying mutations in the Toll signaling pathway as well as in the Imd signaling pathway was more susceptible to PA14 infection. All these results imply that the Toll pathway might be important in the resistance to this pathogenic Gram-negative bacterial infection.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1269-1279
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• 2014

Xanthomonas oryzae pv. oryzae (Xoo) strains are plant pathogenic bacteria that can cause serious blight of rice, and their virulence towards plant host is complex, making it difficult to be elucidated. Caenorhabditis elegans has been used as a powerful model organism to simplify the host and pathogen system. However, whether the C. elegans is feasible for studying plant pathogens such as Xoo has not been explored. In the present work, we report that Xoo strains PXO99 and JXOIII reduce the lifespan of worms not through acute toxicity, but in an infectious manner; pathogens proliferate and persist in the intestinal lumen to cause marked anterior intestine distension. In addition, Xoo triggers (i) the p38 MAPK signal pathway to upregulate its downstream C17H12.8 expression, and (ii) the DAF-2/DAF-16 pathway to upregulate its downstream gene expressions of mtl-1 and sod-3 under the condition of daf-2 mutation. Our findings suggest that C. elegans can be used as a model to evaluate the virulence of Xoo phytopathogens to host.

• Steel and Composite Structures
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• 제31권4호
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• pp.387-396
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• 2019

This paper deals with the effect of ply orientation and control gain on tip transverse displacement of functionally graded beam layer for both active constrained layer damping (ACLD) and passive constrained layer damping (PCLD) system. The functionally graded beam is taken as host beam with a bonded viscoelastic layer in ACLD beam system. Piezoelectric fiber reinforced composite (PFRC) laminate is a constraining layer which acts as actuator through the velocity feedback control system. A finite element model has been developed to study actuation of the smart beam system. Fractional order derivative constitutive model is used for the viscoelastic constitutive equation. The control voltage required for ACLD treatment for various symmetric ply stacking sequences is highest in case of longitudinal orientation of fibers of PFRC laminate over other ply stacking sequences. Performance of symmetric and anti-symmetric ply laminates on damping characteristics has been investigated for smart beam system using time and frequency response plots. Symmetric and anti-symmetric ply laminates significantly reduce the amplitude of the vibration over the longitudinal orientation of fibers of PFRC laminate. The analysis reveals that the PFRC laminate can be used effectively for developing very light weight smart structures.

• 전기학회논문지
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• 제60권2호
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• pp.231-238
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• 2011

A role of Distribution Automation System(DAS) is required to be changed from observing and control to complex and various new system including functions which monitoring, control, prediction, operation and management of EMS type according to be gradually Smart Grid of distribution operation system for development of further power system industrial. IEC(International Electrotechnical Commission) published communication standards which interface and data model of distribution operation system to IEC 61968 and of host system like EMS(Energy Management System) to IEC 61970. So far, each standards are emphasizing themselves, but if they are applied by designed integration of the standards, it could be more efficiency system. So, in this paper, integration application of IEC standards and structure of further distribution operation system to use them are proposed by gradually increasing importance of communication protocols, functions and roles in further distribution management system considering Smart Grid.