Multi-host Pathogenesis by Pseudomonas aeruginosa and Use of Drosophila melanogaster as a New Model Host

  • Cho You-Hee (Department of Life Science, Sogang University) ;
  • Lau Gee (Department of Surgery, Harvard Medical School, Massachusetts General Hospital and Shriner's Burns Hospital) ;
  • Rahme Laurence (Department of Surgery, Harvard Medical School, Massachusetts General Hospital and Shriner's Burns Hospital)
  • Published : 2002.10.01

Abstract

Fruit fly, Drosophila melanogaster has developed efficient immune mechanisms to prevent microbial infection, which are consisted of cellular and humoral responses. During the systemic or local infection, two distinct pathways (Toll and Imd) play major roles in antimicrobial peptide synthesis. The Toll pathway is required to defend Gram-positive bacterial and fungal infections, whereas the Imd pathway is important in Gram-negative bacterial infection. We have shown that the infection of the opportunistic Gram-negative bacterium, Pseudomonas aeruginosa strain PA14 (PA14) into fly dorsal thorax can kill the flies within 48 h ($100\%$ mortality) in our optimized infection condition, suggesting that the PA14 strain can cause disease progress in fly model system. We found that flies carrying a constitutively activated mutant form of the Toll receptor $(Tl^{10b})$ showed increased resistance to P. aeruginosa infection and that flies carrying mutations in the Toll signaling pathway as well as in the Imd signaling pathway was more susceptible to PA14 infection. All these results imply that the Toll pathway might be important in the resistance to this pathogenic Gram-negative bacterial infection.

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