• Journal of Food Hygiene and Safety
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• v.29 no.4
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• pp.340-346
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• 2014

In this study, the detection method was developed using real-time PCR to distinguish 4 species (bovine, porcine, horse, and chicken) of raw meats. The genes for distinction of species about meats targeted at 12S rRNA and 16S rRNA parts in mitochondrial DNA. Probes were designed to have a 5' FAM and a TAMRA at the 3' end. This study is to develop 4 species-specific primer and probes about raw materials and real-time PCR on 10 strains to observe the products of non-specific signal for similar species. As a result, any non-specific signal were not detected among each other. Real-time PCR method was developed for quantitation and identification of intentional and unintentional mixture in ground mixed meat (The difference of $C_T$ value between intentional mixture and 100% meat: $${\leq_-}$$ cycles, The difference of $C_T$ value between unintentional mixture and 100% meat: $${\geq_-}$$ cycles). The detection and differentiation of intentional and unintentional mixture in this study would be applied to food safety management for eradication of adulterated food distribution and protection of consumer's right.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.273-279
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• 2018

This experiment was conducted to investigate the effects of individual management and group management of Jeju cross-bred horses on the physiological characteristics, productivity, and characteristics of the loin muscle of the horse meat. Sixteen herds of Jeju cross-bred horses older than 44 months were selected as experimental subjects. The experiment was conducted by dividing the herds into eight herds for individual management and another eight herds for group management. Herds were fed with concentrated feed of 2.5% of the body weight for four times a day and roughage and water were fed ad libitum. In the 12th week, the intestinal pH was statistically significantly lower with group management than with individual management (p<0.05). However, no symptoms of colic were observed. With regard to the general composition of the loin muscle, fat content was a statistically significant difference between the 7.83% with individual management and 5.65% with group management (p<0.05), indicating that individual management is more effective than group management in terms of fattening. In conclusion, feeding a concentrated feed at a level of 2.5% of body weight with individual management during fattening of Jeju cross-bred horses could be utilized as a fattening method.

• Journal of Aquaculture
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• v.9 no.3
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• pp.255-264
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• 1996

A 15-week growth trial was conducted to evaluate the economical feed formulations for Korean rockfish (Sebastes sehlegeli). Three replicate groups of fish averaging 33 g were fed one of 8 isoproteic ($49\%$) and isolipidic ($8\%$) diets containing various practical ingredients such as fish meal, meat meal, feather meal, blood meal, soybean meal, corn gluten meal, and wheat flour with or without supplemental essential amino acids. A control diet with white fish meal and brown fish meal as the only protein sources was included. Practical ingredients were substituted from $40\%$ up to $65\%$ for portions of the fish meals in the control diet. Essential amino acids (EAA) were added to the diets for balancing EAA composition of each dietary protein source. In addition, these experimental diets were compared with raw fish-based moist pellet containing $50\%$ frozen horse mackerel and $50\%$ commercial binder meal. Results indicated that animal and plant protein sources could substitute for fish meal up to $55\%$ in diet without any adverse effects on growth and chemical composition of fish. Fish growth, body composition, nutrient utilization, and cost of fish production are discussed in relation to nutritional values of the dietary protein sources.

• Journal of Aquaculture
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• v.9 no.4
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• pp.377-384
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• 1996

A 15-week of growth trial was conducted to evaluate practical diets for growing Korean rockfish (Sebastes schlegeli). Two replicate groups of the fish average weighing 125 g were fed one of four experimental diets containing $41\~45\%$ protein and $6\~8\%$ lipid. A control diet with $58\%$ fish meal as protein source was included and practical form diets with $40\%$ fish meal and various practical ingredients such as meat meal, blood meal, soybean meal, and corn gluten meal were included. These experimental dry pellet diets were compared with commercial diets containing $51\~53\%$ protein and $5\~13\%$ lipid for marine fish or raw fish-based moist pellet (RMP) diet containing $58\%$ protein and $9\%$ lipid consisted of $50\%$ frozen horse mackerel and $50\%$ commercial binder meal. Weight gain, feed efficiency, daily feed intake and protein efficiency ratio in the fish fed the experimental diets and commercial diets were not significantly different to those in the fish fed the control diet. However weight gain, feed efficiency and protein efficiency ratio in the fish fed the RMP diet were significantly higher than those in the fish fed the experimental diets and commercial diets. These differences of growth performance between experimental diets and RMP diet may be reason for different dietary protein and energy levels. The experimental diet can save up to $20\%$ cost of fish production compare with control diet, whereas RMP diet is more expensive than other diets in the fish production.