• Korean Journal of Animal Reproduction
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• v.18 no.1
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• pp.63-70
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• 1994

Thirty ewes received typical trauma to their oviducts and uterine horns from surgical embryo collection procedures. Ten percent Dexamethasone was used as an irrigant on the exposed abdominal tissue prior to closing the incision. The treatment group received 17mg colchicine Om! lewe) and the control group was administered a 1.0ml placebo(PSS). Fifteen ewes that were initially treated with 17mg /im colchicine showed acute colchicine toxicity within 2-5 days after initial treatment and were removed from the study. Due to acute colchicine toxicity at 17mg, the colchicine level was lowered to 8, 4 and 2mg(4 ewes/group). Treatments consisted of daily injections of colchicine. One ewe in the 8mg group developed toxicity on day 5. Therefore, ewes were then administered colchicine every other day from day 6 to day 14 postsurgeryat 4 and 2 mg. the second laparotomy was performed 9 weeks after first treatment. Following second laparotomy, the treatment group(n=5) received 4 mg colchicine every day for 14 days and there was no clinical symptoms of colchicine toxicity. The third laparotomy was performed by the same operators 5 weeks after final treatment and the adhesions scored. Adhesion grading was based on a scale of 0-4, with 4 being the most severe. The results of adhesion grading(> 3) at second laparotomy were not significantly different(P>0.05)between the two groups. Adhesion formation observed at third laparotomy showed a reduced, but not significant reduction (P>0.05) in the colchicine-treated ewes when compared with the controls. Ten ewes(5 control and 5 treatment)were examined cytogenetically by bone marrow analysis five days post-treatment. There was no difference(P>0.05)in the incidence of numerical or structural aberrations between the two groups.

• Korean Journal of Animal Reproduction
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• v.22 no.2
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• pp.171-176
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• 1998

This study was carried out to confirm whether the in vivo developmental potential of mouse hatched blastocysts (HBs) can be obtained by vitrification method using the cryoprotectant EFS35. The HBs ($\theta$ 130$\mu\textrm{m}$) were cultured in vitro until day 5 from zygotes produced in vivo and were equilibrated in 10% ethylene glycol(EG) for 5 min, and then were exposed or vitrified in EFS35 (35% EG, 18% Ficoll and 0.3M sucrose). After 30 min thawing, re-expanding HBs were transferred into one or both uterine horns of pseudopregnant recipients on day 3 (4~6 embryos /horn). Pregnancy rates of recipients and implantation were assessed by autopsy on day 15 of gestation. The results obtained in these experiments were summarized as follows : After thaw-ing, in vitro survival of HBs was not significantly different between exposed (65.5%) and vitrified(54.5%) group. Also, when the in vivo development potential was examined, total implantation was not different between control (58.5% ) and vitrified (41.0%) group, although the live fetus formation of vitrified group (24.0%) was significantly lower than that of control (58.3%) group (p< 0.05). These results suggested that vitrification freezing method of mouse HBs using EFS35 can be used to make wide the utility of embryo transfer of the more embryos produced in vitro.

• Korean Journal of Animal Reproduction
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• v.21 no.1
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• pp.47-52
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• 1997

The objective of this study was to investigate the in vitro / in vivo embryonic development after vitrification of mouse zygotes and the chrom osomal normality of delivered live young after embryo transfer. Mouse IVF zygotes were cryopreserved by vitrification using vitrification solution, EFS40 (40% ethylene glyc이, 30% Ficoll a and 0.3 M sucrose in phosphate buffer saline c containing 10% FBS ) . After mouse zygotes were exposed to EFS40 at 25"C for 30 sec., they were immediately plunged into LN$_2$. Vitrified thawed mouse zygotes were cultured upto bIastocysts in M16 for 4 days. The rates of in vitro development were 71.5% under this condition. Cultured blastocysts were transferred to recipients (3 day of pseudopregnant) on one or both uterus horns (6-8 embryos per a uterus horn). And all recipients were allowed to produce litters. The results obtained in these experiments were summarized as follows: The pregnancy rates and in vivo survival rates, live fetus rates, for vitrified zygotes (80.0, 39.6%) were not significantly difference in those of control zygotes (77.8%, 50.0%). Also, all of live-born young mice were chromosomally normal (n=40). This results suggested that proposed rapid vitrification procedures can be effectively use in 1-cell mouse zygotes cryopreservation.

• Journal of Veterinary Clinics
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• v.22 no.3
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• pp.233-238
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• 2005

This study was undertaken to determine the normal serial ultrasonographic appearance of the postpartum uterine involution. Postpartum changes in uterine shape, architecture, echogenicity and diameter were monitored by ultrasonography in 10 Shih-tzu bitches. Serial ultrasonographic examination was done daily during the first week, 3 days interval from 8 to 30 days, and weekly from 31 to 100 days postpartum. All 10 postpartum bitches had normal involution by gross finding, vaginal discharges, and by ultrasonographic findings, uterine shape and echogenicity. The uterine diameter in the placental sites was decreased from $23.56{\pm}1.45$ mm at 1 day to $14.08{\pm}1.55$ mm at 7 day, and in the interplacental sites was decreased from $14.64{\pm}1.28$mm at 1day to $9.61{\pm}1.46$mm at 7 day postpartum. At 65 days postpartum the uterine diameter was 5-6 mm both placental and interplacental sites, and the uterine horns were uniform hypoechic, tubular structures without enlargement. Therefore, complete involution of the uterus occured 65 days. It was concluded that normal postpartum uterine involution in Shih-tzu bitches appeared to be completed 65 days postpartum by gross findings such as vaginal discharges, and ultrasonographic findings. And ultrasonographic characteristics of the postpartum uterine involution were described. Therefore, these result suggest that ultrasonographic assessment is a reliable method for diagnosing the subinvolution of placental sites and uterine dysfunction, such as pyometra in the bitch.

• Journal of Music and Human Behavior
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• v.9 no.2
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• pp.53-67
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• 2012

Improvisational music therapy is known to promote social engagement in children with autism. This study investigated salient features that characterize the engagement of the child with autism and the therapist in improvisational music therapy. Through video analysis of the children's behavior, this study sets out to investigate what engages children with autism into mutual play with the therapist in improvisational music therapy by measuring the shared musical elements between the child and the therapist during musical synchronicity episodes and the children's choice of instruments. A repeated measures, a crossover design was employed in two different conditions (n = 10). Children were randomly assigned into two groups; group 1 participated music therapy first, followed by play therapy second. Group 2 followed the reverse order. Specific target behaviors were analyzed in the selected sessions 1, 4, 8 and 12. As expected, improvisational music therapy produced markedly more and longer events of musical synchronicity in children with autism than the play sessions with toys (p < .001). Rhythm and dynamic appeared to be the two most salient elements during musical synchronicity events between the child and the therapist. Observational findings confirmed that horns and whistles were the most frequently selected instruments in music therapy, whereas a dollhouse set was in play therapy by children with autism. The clinical implications and the details of these findings are discussed further.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.4
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• pp.424-430
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• 2002

Vas deferens is a long tube and could be divided into four regions as its morphological characters: a short and slender proximal region, a convoluted region, a straight and more thick distal region, and an ejaculatory duct, It is connected with the posterior outer horns of the testis and runs to the gonopores opened on the coxa of the fifth walking legs, The proximal region consists almost entirely of simple cuboidal epithelial cells, $12\~28{\mu}$m in height, surrounded by a thin basal membrane. A small aggregation of high cuboidal epithelium is obsened from one side of the proximal region. The convoluted and distal region is composed of two kinds of epithelial cells; high cuboidal epithelial cells, $40\~120{\mu}m$ in height located in dorsal portion and simple cuboidal epithelial cells of $12\~28{\mu}$m in height located in ventral and lateral portion. The ejaculatory duct is surrounded with two kinds of muscle layers, inner longitudinal and outer circular muscle fibers, The lumen is lined with high and simple cuboidal epithelium in almost equal proportions. The proximal region contains mature sperm and basophilic substances. The eosinophilic substances appeared newly going toward the convoluted region and are laid along simple cuboidal epithelial cell layer. Tube-like content (spermatophore) of the vas deferens is ejaculated from a pair of genital pores at mating. Two masses of the content fuse together side by side and are usually deposited on the female thorax between the second and fourth or fifth pereiopods, The spermatophore formed measures $2.7\~4.0$ mm in length and $1.5\~2.7$ mm in width.

• Korean Journal of Weed Science
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• v.7 no.1
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• pp.84-89
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• 1987

This study was conducted to determine the combining effects of a synthetic brassinolide such as homobrassinolide (HBR) with auxin types on the curvature of radish petiole. HBR has no direct effect on the petiole curvature of radish, regardless of concentrations used. Among the natural known plant growth regulators, NAA at 100ppm showed the highest ourvature at 12 hours after treatment. The synthetic auxins such as 2,4-D and MCP resulted in about three times greater effect on curvature than the untreated control, and their effects lasted till 36 horns after treatment, showing much better effect on curvature than that of the natural auxin types of plant growth regulators. The mixture of MCP at 3 ppm with HBR at 100ppm increased petiole curvature of radish seedlings as much as three times greater than that of a single application of HBR. However, the mixture of HBR at 100 ppm plus 2, 4-D at 100ppm increased the petiole curvature of radish seedlings about eight times greater than that of the HBR single application, showing existance of strong synergistic reaction and the most effective combination, and suggesting possibility of a prectical implementation in agriculture.

• Parasites, Hosts and Diseases
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• v.57 no.6
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• pp.657-664
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• 2019

We identified the echinostome metacercariae in Chinese mystery snails, Cipangopaludina chinensis malleata, from Xiengkhuang Province, Lao PDR with morphologies of adult worms recovered. Total 20 snails were examined with artificial digestion method and then the collected metacercariae were orally infected to a mouse and a rat. Adult worms recovered from experimental animals were observed with a light microscope and a SEM. The metacercariae were round, 125×123 ㎛ in average size, with a moderately thick cyst wall, collar spines distributed in the head collar and excretory granules in 2 canals of excretory tube. Adult flukes (3-week-old in a rat) were elongated, ventrally curved and 5.310×1.023 mm in average size. Head collar distinct, bearing 43 collar spines with 5 end group ones on each side. Oral sucker subterminal, prepharynx very short, pharynx well developed, and esophagus relatively short. Cirrus sac well developed, with a saccular seminal vesicle, and ventral sucker very large. Ovary round and on the median line of the body. Testes tandom and elongated. Eggs operculated, elliptical and 90×57 ㎛ in average size. In the SEM observation, the head crown prominent, with 43 collar spines resembled with horns of younger stag. Scale-like tegumental spines were densely distributed on the surface between the head collar and ventral sucker, and their densities were decreased posteriorly. Conclusively, the metacercariae detected in C. chinensis malleata from Lao PDR were identified as those of Echinostoma macrorchis based on the morphological characteristics of adult worms.

• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.102-108
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• 1986

This study was carried out to examine the effects of seasonality, age, abnormalities of reproductive tract and the frequency of superovulatory treatments on the superovulatory responses and rate of pregnancy in repeat breeder for embryo transfer. Thirty eight eggs were obtained from 6 donors by flushing the uterine horns and 33 embryos were transfered to 33 recipients. (29 holstein heifers, 3 holstein cows and 1 Korean native heifer). From this study, the following results could be obtained: 1. It seemed that spring (Feb.-Apr.) was the better season than any others to produce good embryos. Rate of pregnancy decreased significantly after August because of low heating rate. 2. Rate of pregnancy in the cows with normal reproductive tract showed much better superovulatory responses (80%) than the cows with abnormal reproductive tract (33%), as expected. 3. 8 years old cows produced higher rater of pregnancy than 4 years old cow (79.3 vs 50), and also in superovulatory responses, 8 years old cows showed slightly better results than 4 years old cow. 4. The number of ovulated eggs and rate of pregnancy in the repeated superovulatory treatment more than once were 41 eggs and 85.7%, and those of the donors treated the first superovulatory treatment were 32 eggs and 68.4%, respectively. 5. These results are showing the possibility that utilizing the repeat breeders as donors in embryo transfer could be able to elevate their economic values.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.41 no.5
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• pp.543-550
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• 2021

Recently, there has been an increase of accidents related to the rise in the use of Personal Mobility Vehicle (PMV). To solve this problem, the National Assembly announced an amendment that restricted the use of PMV to bicycle roads and to prohibited for people under 13 years old to use PMV, but there is no detailed information about travel speed and safety. In this study, a survey was conducted by dividing the group into minors and adults based on the age of obtaining a driver's license to find out the direction of improvement of laws and systems about PMV. Our results showed that adults considered PMV as more dangerous (Adults 5.50, Minors 4.94) and the suggested age for PMV use was lower from minors than adults (Adults 15.70, Minors 13.85). We found that proper travel speed on bicycle roads differed according to the presence of a driveway (Driveway 26.21 km/h, Non-Driveway 23.55 km/h) and minors had higher a travel speed than adults on all types of bicycle road. Also, Helmets for PMV were seen as the most important safety equipment on all types of bicycle road. and the importance of other safety equipment differed according to the presence of a driveway (Driveway Front-Lighting, Non-Driveway Car Horn). Through this study, It proposes that we have to make new regulations about the use of front lights and horns, as well as enforcement measures that differentiate the speed on each bicycle road type as a way to improve the laws and systems for PMV.