• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2005년도 추계학술발표회 요지
• /
• pp.334-335
• /
• 2005

• 한국식물생명공학회:학술대회논문집
• /
• 한국식물생명공학회 2002년도 춘계학술대회
• /
• pp.142-142
• /
• 2002

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1997년도 추계 학술대회지
• /
• pp.58-59
• /
• 1997

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2019년도 추계학술대회
• /
• pp.133-133
• /
• 2019

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 춘계학술대회
• /
• pp.113-113
• /
• 2022

• Journal of Photoscience
• /
• 제1권1호
• /
• pp.47-52
• /
• 1994

Effects of HgCl$_2$-treatment on electron transport, chlorophyll a fluorescence and its quenching were studied using isolated barley (Hordeum vulgare L. cv. Albori) chloroplasts. Depending on the concentration of HgCI$_2$, photosynthetic oxygen-evolving activities of photosystem II (PS II) were greatly inhibited, whereas those of photosystem I (PS I) were slightly decreased. The inhibitory effects of HgCl$_2$ on the oxygen-evolving activity was partially restored by the addition of hydroxyamine, suggesting the primary inhibition site by HgCl$_2$2-treatment is close to the oxidizing site of PS tl associated with water-splitting complex. Addition of 50 $\mu$M HgCI$_2$ decreased both photochemical and nonphotochemical quenching of chlorophyll fluorescence. Especially, energy dependent quenching (qE) was completely disappeared by HgCl$_2$-treatment as observed by NH$_4$CI treatment. In the presence of HgCI$_2$, F'o level during illumination was also increased. These results suggest that pH gradient across thylakoid membrane can not be formed in the presence of 0 $\mu$M HgCl$_2$. In addition, antenna pigment composition might be altered by HgCl$_2$-treatment.

• 한국작물학회지
• /
• 제39권2호
• /
• pp.193-197
• /
• 1994

Hordeum bulbosum을 이용한 보리 반수체육종법의 실용화를 체계화하기 위해 일련의 실험을 실시하였으며, 유배의 적취시기, 유배의 크기에 따른 배배양 반응, 배지 및 환경조건 등을 조사하였다. 1. 유배의 적취시기는 배발육과 식물체 발생률을 고려할 때 수분 후 17일이 적기였다. 2. 식물체 발생에 유리한 유배의 크기는 0.9mm내외였다. 3. 식물체 발생에 효과적인 배지 및 배양조건은 MS배지의 암상태에서 발생시킨 후 명상태로 하는 것이 정상 식물체 발생률(3%)이 높았으나, 작업상의 편리성을 고려한다면 B5배지에서 24시간의 일장처리조건이 좋았다. 4. 반수체는 정상 2배체에 비하여 배 및 식물체의 발생이 현저히 부진하였다.

• 한국식품조리과학회지
• /
• 제13권5호
• /
• pp.661-668
• /
• 1997

By using several solvents, barley extracts containing the anticomplementary activities in classical pathway were prepared (250 $\mu\textrm{g}$/ml): methanol (83.1%), ethanol (71.9%), water extract (25.4%), M-1 (250 $\mu\textrm{g}$/ml), and the soluble part of methanol extract which showed the highest activity (83.4%) and the yield. Anticomplementary activity of methanol extract as well as protease digestion in classical pathway showed 82.4% and 78.4% in the concentration of 250 $\mu\textrm{g}$/ml, respectively. It was found that protein was not involved in anticomplementary activity in the classical pathway and the methanol extract made an impact on classical pathway, but not on alternative pathway. For the immune-stimulating effect, the T cell proliferation effect of the protease digestion displayed little effect irrespective of the dose. In addition, the T cell proliferation effect of methanol extract showed 13-fold higher proliferation effect compared with positive control. It was revealed that the substance containing protein serves as an important factor for the immune proliferation. Therefore, the anticomplementary activity ${\beta}$-glucan in classical pathway and alternative pathway displayed the lowest activity, showing 2.2%, 22.3% respectively. However, the immune-stimulating effect of ${\beta}$-glucan showed the T cell stimulating effect 13 times higher than positive control.

• Journal of Plant Biotechnology
• /
• 제33권4호
• /
• pp.237-242
• /
• 2006

Commercial cultivars and elite germplasms of barely (Hordeum vulgare L) are still recalcitrant to genetic transformation because of the lack of an efficient regeneration system. In this study, we established an efficient plant regeneration procedure from embryogenic calli derived from mature embryos. Callus induction from germinated mature embryos was best as over 95% in CIM medium (CI medium containing $2.5mg/{\ell}$ dicamba) under dark incubation. Development of embryogenic callus was highest as over 50% in CI3D medium (EC medium supplemented with $3mg/{\ell}$ 2,4-D). The highest regeneration of plants from embryogenic callus (40%) was obtained with CIS medium ($SI+1mg/{\ell}IAA\;and\;2mg/{\ell}\;BA$). These plant regeneration conditions could be useful in improving barley transformation efficiency.

• 한국작물학회지
• /
• 제38권6호
• /
• pp.531-536
• /
• 1993

Hordeum bulbosum에 의한 보리 반수체 이용육종은 단기간에 목적 형질을 고정하는 일반적인 이점 외에 세포학적으로 안정된 많은 반수체를 용이하게 얻을 수 있는 기능성이 있기 때문에 이 방법의 실용화을 제고하기 위한 방법을 체계화하기 위해 일년의 실험을 실시하였으며 우선, H. bulbosum의 여러 형질의 특성 및 재배종과의 교배시 유배형성율등을 조사하였다. H. bulbosum은 H. vulgare에 비하여 간이 가늘고 간장이 길며 세엽이나, 수장이 길고 수당영화수가 2배이상 많으며 수장개화일수가 3배이상 길었으며, H bulbosumrks에는 차이가 적었다. H. bulbosum중 4배체는 2배체에 비하여 유배형성율은 8%, 식물체 발생율은 2배이상 높으나 염색체의 일부가 완전히 소실되지 않으므로 보리의 반수체를 이용한 육종에는 활용할 수 없는 것으로 인정되었다.