Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Somatic Embryogenesis and Plant Regeneration in Barley (Hordeum vulgare L.)

  • Park, Tae-Il (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Um, Mi-Ok (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Young-Jin (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Hwang, Jong-Jin (Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Choi, Kyung-Gu (Faculty of Biological Resources Science, and Institute of Agricultural Science and Technology, Chonbuk National University) ;
  • Yun, Song-Joong (Faculty of Biological Resources Science, and Institute of Agricultural Science and Technology, Chonbuk National University)
  • Published : 2006.12.30
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Abstract

Commercial cultivars and elite germplasms of barely (Hordeum vulgare L) are still recalcitrant to genetic transformation because of the lack of an efficient regeneration system. In this study, we established an efficient plant regeneration procedure from embryogenic calli derived from mature embryos. Callus induction from germinated mature embryos was best as over 95% in CIM medium (CI medium containing $2.5mg/{\ell}$ dicamba) under dark incubation. Development of embryogenic callus was highest as over 50% in CI3D medium (EC medium supplemented with $3mg/{\ell}$ 2,4-D). The highest regeneration of plants from embryogenic callus (40%) was obtained with CIS medium ($SI+1mg/{\ell}IAA\;and\;2mg/{\ell}\;BA$). These plant regeneration conditions could be useful in improving barley transformation efficiency.

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References

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