• Korean journal of applied entomology
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• v.36 no.2
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• pp.119-125
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• 1997

In a survey of spiral nematode (Hoplolaimidae) in Korea, Helicotylenchus cavenessi sher, 1996, Helicotylenchus paraplatyurus Siddiqi, 1972, Rotylenchus alius Van den Berg, 1986 and Rotylenchus incultus Sher, 1965 were newly found and recorded in Korea.

• Korean journal of applied entomology
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• v.34 no.3
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• pp.224-228
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• 1995

During the survey on spiral nematode (Hoplolaimidae) from Korea, Pararotylenchus blothrotylus Baldwin & Bel, 1981, Rotylenchus feroxcis Eroshenko, 1981 and Scutellonema conicephalum Sivakumar, 1982 were newly recorded from Korea.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.171-191
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• 2024

Identification of Helicotylenchus species is very challenging due to phenotypic plasticity and existence of cryptic species complexes. Recently, the use of rDNA barcodes has proven to be useful for identification of Helicotylenchus. Molecular markers are a quick diagnostic tool and are crucial for discriminating related species and resolving cryptic species complexes within this speciose genus. However, DNA barcoding is not an error-free approach. The public databases appear to be marred by incorrect sequences, arising from sequencing errors, mislabeling, and misidentifications. Herein, we provide a comprehensive analysis of the newly obtained, and published DNA sequences of Helicotylenchus, revealing the potential faults in the available DNA barcodes. A total of 97 sequences (25 nearly full-length 18S-rRNA, 12 partial 28S-rRNA, 16 partial internal transcribed spacer [ITS]-rRNA, and 44 partial cytochrome c oxidase subunit I [COI] gene sequences) were newly obtained in the present study. Phylogenetic relationships between species are given as inferred from the analyses of 103 sequences of 18S-rRNA, 469 sequences of 28S-rRNA, 183 sequences of ITS-rRNA, and 63 sequences of COI. Remarks on suggested corrections of published accessions in GenBank database are given. Additionally, COI gene sequences of H. dihystera, H. asiaticus and the contentious H. microlobus are provided herein for the first time. Similar to rDNA gene analyses, the COI sequences support the genetic distinctness and validity of H. microlobus. DNA barcodes from type material are needed for resolving the taxonomic status of the unresolved taxonomic groups within the genus.