• 대한임상검사과학회지
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• 제41권4호
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• pp.173-179
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• 2009

The analysis of serum free light chains (sFLCs) can improve the diagnosis and monitoring of multiple myeloma and other plasma cell dyscrasias. As with other immunoassays, sFLCstests are subject to potential antigen excess and heterophilic antibody interference. We describe 9 cases of sFLCs antigen excess in patients with multiple myeloma using the FreeliteTM Human Kappa and Lambda Free Kits (The Binding Site ltd., Birmingham, UK) and the Hitachi7600 P module turbidimetric system. A total of 1,247 consecutive samples from 250 patients with multiple myeloma were assayed for sFLCs from April to September, 2009. The samples were assayed using an initial dilution of 1 :5and subsequent dilutions of 1 :50 and 1: 100. The same samples were analyzed for the presence of monoclonal gammopathies using serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE). There were 9 samples (0.72%) of antigen excess with 3 cases of kappa (0.24%) and 6 cases of lambda (0.48%). These cases represents an example of antigen excess or "hook effect" using the serum free light chain assays and mandates high level of attention to falsely low sFLC levels due to antigen excess, especially when it is disaccordant to other assay results or clinical manifestations.

• 핵의학기술
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• 제12권1호
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• pp.66-73
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• 2008

Purpose: CA 19-9 need to examine a number of sample volume, and the postwar 200 U/ml concentration hook effect appears slight. Thus, the antibody-antigen reaction, and by reducing the amount of (sample volume), they can hook effect to minimize the impact of the sample volume and relevance know, I saw the hook effect. In addition, the current maximum of using the standard concentration of the reagent in 240 U/ml increase more than the standard concentration can be seen knows. Material and Method: 5 U/ml and under, make a few low concentration of serum pool from the high concentration of the sample hook effect together with a standard concentration of about 500 to meet the production. The reagents used in experiments are currently using SNUH NM experiment. Orignal method along with the experiment is to 25 ul sample volume (1 / 4), 50 ul (1 / 2), 100 ul (Orignal method) in the experiment. My greatest concentration of the reagent concentration of approximately two times the standard concentration of production. When was the last to make the first experiment, as measured by the standard concentration after that. The new inspection information through a standard solution modified by entering values in them. Results: 100 ul, and to apply the new standard concentration y = 1.3021x - 10.97, $R^2$ = 0.9844. Overall, the results showed a similar orignal method. Because of the concentration in the value of more than 240 U/ml, but it is an overall value that can be made out of a similar value When I put the 50 ul y = 1.045x + 9.5861, $R^2$ = 0.9428. Overall orignal method and the results of a similar value. 50 ul, and to apply the new standard concentration y=1.2006x+11.252, $R^2$=0.9423. Showing a slightly lower value compared with orignal method. Because of the concentration in the value of more than 240 U/ml, but it is an overall value that can be made out of a similar value. When I put the man 25 ul y=0.6012x+24.755, $R^2$=0.4033. Results showed that very small amounts of sample are insecure inside and showed a lower middle cpm orignal method and showed a lot of mismatched. Conclusions: 25 ul of the sample volume is not possible to use the instability had, when I put the 50 ul of the orignal method can be used to show a similar concentration. The new values are slightly lower concentration, The new values are slightly lower concentration, concentration, which are likely due to the lack of data has had a little gap between the sample showed 80 to 200 U/ml additional experiments seem to do. Apply a new 100 ul concentration values are applied to a large crowd is not even in sight. But this way the concentration of 100 to more 400 U/ml gather further experiments should possible adds.

• 대한기계학회논문집A
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• 제32권2호
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• pp.162-169
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• 2008

Compliance effect consideration method for real-time multibody vehicle dynamics is proposed using quasi-static analysis. The multibody vehicle model without bush elements is used based on the subsystem synthesis method which provides real-time computation on the multibody vehicle model. Reaction forces are computed in the suspension subsystem. According to deformation from the quasi-static analysis using reaction forces and bush stiffness, suspension hardpoint locations and suspension linkage orientation are changed. To validate the proposed method, quarter car simulations of McPherson strut and multilink suspension subsystems are performed. Full car bump run simulations and fish hook handling test simulations are also carried out comparing with the ADAMS vehicle model with bush elements. CPU times are also measured to see the real-time capabilities of the proposed method.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.201.3-201.3
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• 2003

The ethanol extract from the fruit of Terminalia chebula (Combretaceae) and the hook of Uncaria sinensis (Rubiaceae) exhibited significant inhibitory activity on oxidative stress and the age-dependent shortening of the telomeric DNA length. In the peroxidation model using t-BuOOH, human epidermal keratinocytes-neonatal foreskin (HEK-N/F) cells were treated with the T. chebula and U. sinensis extracts. The results showed a notable enhancing effect on the cell viability of 60.5 ${\pm}$ 3.8 and 65.0 ${\pm}$ 3.0%, respectively, by 50 $\mu\textrm{g}$/ml of the extracts. (omitted)

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 춘계학술대회논문집
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• pp.746-751
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• 2002

This purpose of this study was to design the effect of recovering of a hand amputees by myoelectronic hand. It was designed with 2 degree of freedom in tile laboratory. Myoelectronic hand had only one degree of freedom and one movement until now. Also this myoelectronic hand had multi-joint system. Myoelectronic hand data was obtained by measuring hand and data was applied when it was designed myoelectronic hand. PID controll of myoelectronic hand was used to it. Displacement control was applied the first link of finger. Experiment was accomlkished in Tip grasp, power grasp and Hook grasp modes. Displacement controll was good in low frequency. Velocity control was applied to each mode. This myoelectronic hand with a hand amputees could do some jobs such as grasping materials. Further studies were needed to evaluate the effect of a myoelectronic hand with more precise laboratory equipment.

• 대한물리치료과학회지
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• 제9권4호
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• pp.155-168
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• 2002

The purpose of study to know hand function in order to prevent disability or handicap. The ability to perform precise refined movements of hand is an important human function. Improvement in object manipulation is common goal of therapist. The ability to manipulate an object in the hand is need for many functional tasks, including writing, handling coins, small objects and ADL skills. Therapists have commonly used hand grip and pinch strengths as baseline measures to evaluate hand function. The patterns of grasps are precision grasp, power grasp, hook grasp, spherical grasp, sylindrical grasp, disc grasp, pinch, three point pinch and tip pinch. And the motion of in-manipulation are finger to palm translation, palm to finger, shift, simple rotation and complex rotation. The hand function are include to evaluate of ROM, sensation, muscle strength of hand. It used to evaluate of decision of effect and suppose of disability and acceptance of vocation. Good evaluation is need to pretreatment and baseline of treatment and help to evaluate of effect on treatment.

• 한국구조물진단유지관리공학회 논문집
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• 제24권4호
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• pp.72-80
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• 2020

이 연구의 목적은 철근콘크리트 기둥에서 외부띠철근 상세로 이용되는 갈고리 양단 135도 교차 시공 상세에 대해, 대등한 구조적 성능을 확보하면서, 동시에 시공성을 개선할 목적으로 클립형 연결장치로 띠철근 갈고리 보강하는 방법을 제시하였다. 제안한 클립형 연결장치로 결속한 띠철근의 정착거동 및 강도를 파악하기 위해 콘크리트 압축강도, 클립형 연결장치의 묻힘길이, 설치 위치를 주요 변수로 28개 인발 실험체를 제작하여 실험하였다. 실험결과, 정착강도는 콘크리트 압축강도, 띠철근 직경, 클립의 묻힘길이, 설치 위치에 관계없이 클립형 연결장치로 결속하는 경우, 표준 갈고리 상세보다 모두 높게 나타났고 표준 갈고리 상세의 정착거동과 유사한 거동을 보여주었다. 그러므로, 90도 갈고리에 클립형 연결장치로 결속하는 상세는 표준 갈고리 상세와 대등한 정착거동 및 성능을 발휘할 수 있는 것으로 평가할 수 있다.

• 정보처리학회논문지D
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• 제19D권3호
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• pp.211-220
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• 2012

최근 들어 K-POP의 인기가 국내 뿐 아니라 외국에서도 선풍적인 인기를 모으고 있다. 이 이유는 한 곡에서 똑같은 멜로디의 반복이 70여번까지 행해지는 등 귀에 박힐 듯이 반복되는 후크송이라는데 특징이 있다. 여기에 군무 등 시각적 요소 등이 가미되면서 인기를 끌고 있다. 본 논문에서는 60년대부터 K-POP에 이르기까지 우리나라 가요의 시대별 선호이유를 음향 신호 분석기술을 적용하여 분석하는 방법을 제안하고자 한다. 이를 위해 각 시대별로 유행했던 여자 가수들의 가요를 중심으로 음향 신호 분석을 통해 한국가요의 선호도가 어찌 변해 왔는지를 규명하는 실험을 행하고자 한다. 음향신호분석 기술을 적용하여 실험을 행한 결과 한국가요의 선호도에 대한 과학적 규명 및 이를 시대별로 구분 짓는 작업이 가능했으며 기존에 한국 가요의 선호 이유에 대한 주관적, 통계적 자료를 음향 신호 분석의 정량적 잣대로 객관화, 수치화를 행할 수 있었다.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.157-163
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• 2010

A protocol for in vitro propagation from pseudobulb sections of Lycaste armomatica (Graham ex Hook) Lindl., an ornamental and fragrant orchid, was developed. The effect of four cytokinins: kinetin (K), metatopolin (mT), $N^6$-benzyladenine (BA), and thidiazuron (TDZ), in equimolar concentrations, was investigated. Shoot formation from apical and basal pseudobulb sections was obtained in all treatments. A few medial sections cultured in media supplemented with BA formed protocorm-like bodies. Shoot formation was greater from the basal section than the apical, and mainly occurred in explants cultured in media containing TDZ. The highest average numbers of shoots per explant were achieved from basal sections cultured in media supplemented with TDZ at 4.4, 8.87 and 2.2 ${\mu}M$, forming on average 9.9, 8.6 and 7.3 shoots per explant, respectively. Since the medial pseudobulb section was the worst explant for propagation of L. aromatica, we recommend that pseudobulbs be divided into two sections; the basal half should be cultured in MS medium supplemented with TDZ at 4.4 ${\mu}M$ and the apical half with TDZ at 2.2 ${\mu}M$. Subculturing individual shoots in MS medium without plant growth regulators allows further development and rooting. A survival rate of more than 90% under greenhouse conditions was achieved. This research represents a direct contribution to the conservation and sustainable use of this valuable natural resource.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.823-834
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• 2014

In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of $50{\mu}M$ methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas $50{\mu}M$ salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of $500{\mu}M$ had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment.