• Title/Summary/Keyword: Homogenization Method

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Effect of Extraction Methods on the Types and Levels of Free Amino Acid of Beef Longissimus Muscle

  • Dashdorj, Dashmaa;Hwang, In-Ho
    • Food Science of Animal Resources
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    • v.32 no.3
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    • pp.369-375
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    • 2012
  • The current study was carried out to investigate the impact of extraction conditions on the free amino acid level and type in beef longissimus muscle. The sample blocks were chiller aged for 1 d and 7 d at $4^{\circ}C$. There are three homogenization speeds (11,000, 19,000 and 24,000 rpm) for bigger and two speeds (11,000 and 13,000 rpm) for smaller homogenizer's dispersing tools were used for evaluation. Results showed that chiller ageing greatly (p<0.05) increased extractable free amino acids, except cystine. Homogenization with the bigger dispersing tool at 24,000 rpm resulted in the highest free amino acid levels for both 1 and 7 d samples. Significant differences (p<0.05) in the mean values of most amino acids due to the effect of speed and interactions between ageing times. However, the speed effect and interaction between ageing with homogenization speed were not significant (p>0.05) for most of the amino acids except valine and isoleucine when using the smaller dispensing tools. The current data indicated that a standardized method for free amino acid types and levels of aged beef samples. In addition, the results also suggested that utilization of a big dispensing tool at high homogenization speed is a better condition for releasing free amino acid contents in beef samples.

The Microstructural Evolution of Mg-10Al-Mn Alloy by Cooling Plate During Homogenization Treatment (냉각판법에 의한 Mg-10Al-Mn 합금의 균질화 처리에 따른 미세조직 변화)

  • Kim, Dae-Hwan;Choi, Seung-Hwa;Kim, Hee-Kyung;Shim, Sung-Young;Lim, Su-Gun
    • Journal of Korea Foundry Society
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    • v.30 no.6
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    • pp.235-240
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    • 2010
  • The evolution of microstructure and phases of Mg-10Al-Mn alloy by cooling plate method during homogenization treatment have been investigated with metallographic analysis, scanning electron microscopy and energy dispersive spectroscopy. The ingots used for this experiment were prepared by cooling plate and homogenization heat treatment was performed at 300 and $400^{\circ}C$ for various holding times (0, 1, 4, 8 and 12h). The casting ingots were consisted of the fine grains and eutectic phases. And, these eutectic phases were dissolved into the matrix during homogenization treatment at $400^{\circ}C$ but the lower temperature (at $300^{\circ}C$) did not be.

Simplified stress analysis of perforated plates using homogenization technique (균질화기법을 이용한 다공평판의 단순화된 응력해석)

  • 이진희
    • Computational Structural Engineering
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    • v.8 no.3
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    • pp.51-57
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    • 1995
  • A simplified stress analysis of perforated plates was carried out using homogenization technique. Homogenization technique, which introduced miroscale expansion in the standard finite element method, reconstructed the plate with regularly placed holes into a set of macroscale and microscale models. The microscale model helped compute homogenized material constants of the unit cell, which were used to compute macroscale displacements in the macroscale model. Also it was possible to compute the stress field of the plate using the microscale model. It was found that reasonable equivalent material constants were computed and that the required degrees of freedom was drastically reduced when homogenization technique was employed in the stress analyses. The microscale modeling in the homogenization technique provided a useful concept of pre- and post-processing in the stress analysis of perforated plates.

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Optimization of Preparation Variables for Trimyristin Solid Lipid Nanoparticles

  • Choi, Mi-Hee;Lee, Mi-Kyung
    • Journal of Pharmaceutical Investigation
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    • v.37 no.1
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    • pp.51-55
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    • 2007
  • Solid lipid nanoparticles (SLNs) have been regarded to behave similar to the vegetable oil emulsions because emulsions of lipid melts are formed before lipid droplets being solidified to turn into SLNs. Compared to lipid emulsion, however, it has been more difficult to obtain stable SLNs and needs more extensive considerations on stabilizer and manufacturing process. In the present study, we tried to prepare phosphatidylcholine-based trymyristin (TM) SLNs using high pressure homogenization method and optimize the manufacturing variables such as homogenization pressure, number of homogenization cycles, cooling temperature, co-stabilizer and freeze-drying with cryoprotectants. Nano-sized TM particles could be Prepared using egg Phosphatidylcholine and pegylated phospholipids ($PEG_{2000}$PE) as stabilizers. Based on the optimization study, the dispersion was manufactured by homogenization under the pressure of 100 MPa for more than 5 cycles, and solidifying the intermediately formed lipid melt droplets by dipping in liquid nitrogen followed by thawing at room temperature. In addition, TM SLNs could be freeze-dried and then redispersed easily without significant particle size changes after freeze drying with 10% and 12.5% sucrose or trehalose. The TM SLNs established in this study can be used as delivery system for drugs and cosmetics.

Enthalpy - based homogenization procedure for composite piezoelectric modules with integrated electrodes

  • Kranz, Burkhard;Benjeddou, Ayech;Drossel, Welf-Guntram
    • Smart Structures and Systems
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    • v.12 no.5
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    • pp.579-594
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    • 2013
  • A new enthalpy - based procedure for the homogenization of the electromechanical material parameters of composite piezoelectric modules with integrated electrodes is presented. It is based on a finite element (FE) modeling of the latter's representative volume element (RVE). In contrast to most previously published homogenization approaches that are based on averaged quantities, the presented method uses a direct evaluation of the electromechanical enthalpy. Hence, for the linear orthotropic piezoelectric composite behavior full set of elastic, piezoelectric, and dielectric material parameters, 17 load cases (LC) are used where each load case leads directly to one material parameter. This gives the possibility to elaborate a very strict and easy to program processing. In conjunction with the 17 LC, the enthalpy - based homogenization is particularly suitable for laminated composite piezoelectric modules with integrated electrodes. In this case, the electric load has to be given at the electrodes rather than at the RVE FE model boundaries. The proposed procedure is validated through its comparison to literature available results on a classical 1-3 piezoelectric micro fiber (longitudinally polarized) reinforced composite and a $d_{15}$ shear piezoelectric macro-fiber (transversely polarized) composite module.

Modeling of the Mechanical Behavior of Polycrystalline Shape-Memory Alloys by a Homogenization Method (균질화법에 의한 다결정 형상기억합금의 기계적 거동 모델링)

  • Gu, Byeong-Chun;Jo, Jin
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.24 no.5 s.176
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    • pp.1352-1361
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    • 2000
  • We obtain a micromechanics-based Helmholtz free energy and then in the framework of irreversible thermodynamics, a kinetic relation, a martensitic nucleation criterion and the reorientation criterion of martensitic variants are obtained. These relations are valid for a three-dimensional proportional and non-proportional loadings and for a combination of mechanical and thermal loading. From the simulated pseudoelastic stress-strain relation of a single crystal with loading rate effect, polycrystalline behavior in case of proportional and non-proportional loading is predicted by a homogenization method. The obtained results are compared quantitatively with experimental results.

Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection

  • Duong, Thanh Van;Shin, Dong-Il;Park, Hee-Sung
    • The Plant Pathology Journal
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    • v.26 no.3
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    • pp.286-288
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    • 2010
  • Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.

Dynamic Behavior Analysis of a Satellite Having Sandwich Panel by Utilizing Asymptotic Homogenization (Asymptotic 균질화법을 활용한 샌드위치패널로 제작된 위성 구조체의 동적 거동 분석)

  • Cho, Hee Keun
    • Journal of the Korean Society for Precision Engineering
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    • v.30 no.11
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    • pp.1203-1210
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    • 2013
  • Korea's first Naro-Science small class satellite was launched by Naro launcher in 2013. The structure of the satellite is mostly composed of aluminum honeycomb and frame. The honeycomb structure is homogenized with asymptotic homogenization method and its mechanical properties were used for the numerical analysis. There have been some difficulties to modeling the honeycomb sandwich panels for FEA. In the present study, the mechanical characteristics of the sandwich panel composite were numerically computed and used for the simulation. This methodology makes it easy to overcome the weakness of modeling of complicated sandwich panels. Both an experiment of vibration test and numerical analyses were conducted simultaneously. The analysis results from the current homogenization were compared with that of experiment. It shows a good agreement on the dynamic responses and certified the reliability of the present methodology when manipulate sandwich panel structure.

Characterization of the mixed soil with waste and application to geotechnical field (폐기물을 포함한 혼합토의 특성 및 지반공학분야에의 응용)

  • 이기호
    • Proceedings of the Korean Geotechical Society Conference
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    • 2001.11a
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    • pp.72-84
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    • 2001
  • In order to utilize mass of oyster shells for a partial substitute material for reclamation, we investigate the shear characteristics of dredged sluge mixed with oyster shells. the apparent modulus of elasticity of the this mixture are obtained from the triaxial compression tests and is utilized to characterize the apparent modulus of elastic of the oyster shells by carrying out some numerical analysis based upon the homogenization theory. We got the conclusion by a series of experiment, 1) It is verified that modulus of elasticity of dredged clay is improved by mixing with oyster shells. 2) The homogenization method for deducing apparent modulus of elasticity of oyster shells, which can consider micro-structure of mixed soil, is introduced. The elastic modulus is affected from the skeleton structure of oyster shell. The effect of 49kPa is bigger than that of 98kPa.

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