• 대한치과교정학회지
• /
• 제38권2호
• /
• pp.133-143
• /
• 2008

이 연구는 한국인 비증후군성 구순구개열자에서 구순구개열과 치아결손의 중요한 원인 중 하나로 의심되는 MSX1 유전자(locus chromosome 4p16)의 특성을 밝히기 위해 시행되었다. 1998년부터 2002년까지 부산대학교병원 치과교정과에 내원한 36명(남자:23, 여자:13)의 비증후군성 구순수 개열자를 대상으로 하였다. 모든 대상의 혈액을 채취하여 중합 효소연쇄반응(polymerase chain reaction)에 기초한 유전자 분석을 시행하여, MSX1 유전자를 증폭하고, 염기서열을 분석하였으며, 추론되는 단백질 생성물에 대해서도 연구하였다. 이미 밝혀진 Homo sapiens MSX1, accession number AF426432와 NP_002439를 참고로 하여 비교 분석한 결과 공통적인 단일 염기 다형성이 존재하였다. exon 1에서, 253번째 부위의 염기 "A"가 "G"로 치환되었고, 255번째 부위에서 염기 "G"가 삽입되었다. exon 2에서 11번째 부위에서 염기"C"가 "A"로 치환되었고, 351부위에서 염기"T" 또는 "G"가 삽입되었고 352부위에서 염기"T" 또는 "A"가 삽입되었다. 한국인 비증후군성 구순구개열자에서 다른 인종에서 발견된 돌연변이와는 다른 "Thr85A1a" missense 돌연변이가 발견되었다. 이는 한국인 비증후군성 구순구개열에서도 MSX1 유전자가 중요한 원인이 될 수 있고 한국인의 독특한 돌연변이가 존재한다는 가능성을 제시한 것이다. 그러나, 구개열 부위의 치아결손과 관련해서는 어떠한 유전자 특징도 관찰되지 않았다.

• Archives of Pharmacal Research
• /
• 제26권10호
• /
• pp.846-854
• /
• 2003

The oligomerization of G-proteincoupled receptors (GPCRs) has been shown to occur by various mechanisms, such as via disulfide covalent linkages, non covalent (ionic, hydrophobic) interactions of the N-terminal, and/or transmembrane and/or intracellular domains. Interactions between GPCRs could involve an association between identical proteins (homomers) or non-identical proteins (heteromers), or between two monomers (to form dimers) or multiple monomers (to form oligomers). It is believed that muscarinic receptors may also be arranged into dimeric or oigomeric complexes, but no systematic experimental evidence exists concerning the direct physical interaction between receptor proteins as its mechanism. We undertook this study to determine whether muscarinic receptors form homomers or a heteromers by direct protein-protein interaction within the same or within different subtypes using a yeast two-hybrid system. Intracellular loops (i1, i2 and i3) and the C-terminal cytoplasmic tails (C) of human muscarinic (Hm) receptor subtypes, Hm1, Hm2 and Hm3, were cloned into the vectors (pB42AD and pLexA) of a two-hybrid system and examined for heteromeric or homodimeric interactions between the cytoplasmic domains. No physical interaction was observed between the intracellular domains of any of the Hm/Hm receptor sets tested. The results of our study suggest that the Hm1, Hm2 and Hm3 receptors do not form dimers or oligomers by interacting directly through either the hydrophilic intracellular domains or the C-terminal tail domains. To further investigate extracellular domain interactions, the N-terminus (N) and extracellular loops (o1 and o2) were also cloned into the two-hybrid vectors. Interactions of Hm2N with Hm2N, Hm2o1, Hm2o2, Hm3N, Hm3o1 or Hm3o2 were examined. The N-terminal domain of Hm2 was found to have no direct interaction with any extracellular domain. From our results, we excluded the possibility of a direct interaction between the muscarinic receptor subtypes (Hm1, Hm2 and Hm3) as a mechanism for homo- or hetero-meric dimerization/oligomerization. On the other hand, it remains a possibility that interaction may occur indirectly or require proper conformation or subunit formation or hydrophobic region involvement.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권12호
• /
• pp.1897-1902
• /
• 2018

Objective: This study was conducted to evaluate the effect of homo or hetero fermentative inoculants on fermentation quality and aerobic stability of sweet potato vine (SPV) silage containing Italian ryegrass hay as moisture absorbent. Methods: The SPV was harvested at 15% dry matter, mixed with Italian ryegrass hay at 1:1 ratio on a fresh weight basis, and chopped to 3 to 5 cm length. After then, the chopped forage mixture was ensiled into 20-L mini silos in quadruplicate for 7, 48, and 100 days after application of microbial inoculants at $1.2{\times}10^5$ colony forming units (cfu)/g of forage following: no inoculant (CON), Lactobacillus plantarum as a homo fermentative (LP), Lactobacillus buchneri as a hetero fermentative (LB), and mixture of LP and LB at 1:1 ratio as a combo fermentative (MIX). Results: The LP and MIX silages had lowest pH (p<0.001) on 7 and 48 days, while MIX and CON silages had greatest lactate concentrations (p<0.05) on 7 and 48 days, respectively. Acetate concentrations were highest (p<0.01) in LB and MIX silages on 7 days, and in LB silage on 48 days, while lactate to acetate ratios were lowest (p<0.001) in LB silages. The chemical compositions and nutrient digestibility of silage ensiled for 100 days was not affected by inoculants. On 100 days of ensiling, LB silage had lowest (p<0.01) lactate concentration and lactate to acetate ratio, but highest acetate concentration. Aerobic stability was highest (p<0.001) in LB silage followed in MIX silage. On contrast, LB silage had lowest (p<0.05) lactic acid bacteria and mold. Conclusion: The results indicated that application of LB solely had a better effect on aerobic stability than not only LP, but also MIX. However, LP application did not show beneficial effects from the viewpoints of fermentation quality and aerobic stability compared to CON.

• 셀메드
• /
• 제2권1호
• /
• pp.4.1-4.13
• /
• 2012

When medicine is unable to cure, and the end becomes imminent, or when the patient is tired of the side effects associated with chronic use of drugs, the search for alternative and new ways of healing is begun. Coincidentally, sometimes the alternative is the origin, as is the case for traditional Arab medicine and traditional American Indian healing. Traditional healing is the first healing that all people have used for 200,000 years, since the beginning of Homo sapiens. The sources and elements of traditional Arab medicine have been examined in books and by consulting with traditional Arab healers. Arabic medicine is a career combining both elements of science and philosophy based on religion and traditions, and includes a diversity of healing approaches: spiritual, physical, and using natural products. These approaches are discussed with emphasis on wet cupping (Alhijamah), a practice that is undergoing a revival nowadays in Arab countries. American Indian healing is a career based on religion, tradition, an innate healing gift and extensive training, both in a medical school setting and as an apprentice. Arabic healing approaches are compared to American Indian healing approaches.

• 턱관절균형의학회지
• /
• 제8권1호
• /
• pp.6-10
• /
• 2018

In order to propose a fundamental and appliable theories for balancing therapy of temporomandibular joint (TMBT), evolutionary proofs and up-down theories in evolutionary biology and Korean medicine were investigated. Balancing therapy of temporomandibular joint treats disorder and diseases of the whole body through straightening of the abnormal linking between temporomandibular joint and axis. Although the mechanism of this therapy contains many merits like multicellular integrity and coadjustment, ease of balance and alert forward mobility by the bipedal stepping and evolution to Homo sapiens, increasing disadvantages of balancing pressure of right and left in the lengthened perpendicular axis and the balancing load of temporomandibular joint and axis following the reactional change of dental occlusion are deeply related and considered in this therapy. As for up-down theory, crossing of heavenly qi and earth qi centering on cervical joint is presented as the first mechanism for TMBT, and the other ones like in-out and up-down qi activity of tripple energizer, up-down of essence-qi-spirit in the three backbone barrier and three cinnabar field, up-down of yin-yang-water-fire of viscera and bowels can be related too.

• 영어영문학
• /
• 제58권4호
• /
• pp.679-700
• /
• 2012

This study aims to examine Mark Twain's criticism of American capitalistic ideals in the late nineteenth century. During this second industrial revolution, industry showed rapid growth and capitalism established an order, while America suffered under the monopolization of capitalistic conglomerates. This resulted in the widening gap between the rich and the poor and the dehumanization caused by rapid industrialization. In A Connecticut Yankee in King Arthur's Court, Hank Morgan, the protagonist--who represents nineteenth-century America's industrialism, individualism, and capitalism--is sent back in time to the sixth century of Arthurian England. Hank attempts to introduce nineteenth-century technologies and machines to build a capitalistic system in the middle ages. However, Hank's efforts lead to disaster in which the country and civilization he worked to build is completely destroyed. Although Twain does not deny capitalistic ideals, he criticizes the "governmentality" that operates Hank's reform system to the extreme. Hank values efficiency and utilizes human beings as capital. Hank's economic reason not only transforms the Round-Table knights into speculators but also transforms their religious acts and abstract ideals into moneymaking businesses. The destructive ending anticipates the World Wars and the Great Depression in the first half of twentieth century and even serves to predict the dangers that follow.

• 영어영문학
• /
• 제56권6호
• /
• pp.1281-1294
• /
• 2010

In Written on the Body, by creating the narrator's ungendered and unsexed identity, Winterson makes her text open to the reader's assumption of the narrator's sexual and gender identity. Thus, this novel has been read, on the one hand, as a lesbian text by those who assume that the narrator is a female and, on the other hand, as a suspicious text colluding with patriarchal and heterosexual values by those who define the narrator as a male. Those readings of the narrator as one of either sex/gender, however, demonstrate how (academic as well as general) readers have been accustomed to the gender-based reading habits in which textual meanings are dichotomously arranged along the lines of sex and gender of characters. Challenging those dualistic "gendered" readings, this paper reads Winterson's Written on the Body as a queer text which interrogates, troubles, and subverts the heterosexual concepts of narrative, desire, and body without reducing the narrator's identity to the essentialist sex and gender system. More specifically, this paper examines how the narrator's 'un-/over-' determined sexual and gender identity queers the narrative structure of author-character-reader; how the narrator's queer (fluid) desire is passing and traveling across categorical contours of (homo-/hetero-) sexual desires; how Winterson challenges the concept of a coherent body and queers the concept of body as a hermeneutic text with myriad textual grids which are not coherently mapped by power but randomly inscribed by nomadic desires.

• 한국콘텐츠학회논문지
• /
• 제18권9호
• /
• pp.510-520
• /
• 2018

본 연구는 이성애규범주의를 기반으로 하는 사회문화적 맥락 내에서 이성애여성이 남성동성애만화를 수용하는 현상을 여성심리학 관점에서 고찰하였다. 먼저, 남성동성애만화의 국내 전개 과정과 확산 현황을 탐색하였다. 다음으로는 남성동성애만화의 장르적 특성에 대한 쟁점, 남성동성애만화 독해의 심리적 기제와 과정, 그리고 남성동성애만화 수용의 기능과 효과를 논의하였다. 이론적 분석틀로서 고전적 정신분석학을 출발점으로 하여 주요 페미니즘 이론, 퀴어이론, 그리고 Huizinga의 호모루덴스 개념을 비판적으로 채택하였다. 연구결과를 보면, 남성동성애만화는 이성애여성을 위한 로맨스로서 영원한 사랑과 평등한 애정관계라는 소망을 성취해주는 판타지로 장르화 된다. 이성애여성은 남성동성애만화를 통해 이성애가부장제로부터 심리적 탈맥락화를 실현하며, 텍스트 속 캐릭터와 분리되어 관음적 욕구를 채우기도 하고, 또는 젠더전복적 동일시를 통해 이분법적 성별 체계를 교란시키는 시도를 하기도 한다. 이러한 심리적 과정은 성 엄숙주의에 도전하는 유희로 간주될 수 있으며, 궁극적으로 여성의 성적 주체성에 대한 자각을 강화시킨다고 하겠다.

• 한국결정학회:학술대회논문집
• /
• 한국결정학회 2002년도 정기총회 및 추계학술연구발표회
• /
• pp.4-4
• /
• 2002

PDZ domains bind to short segments within target proteins in a sequence-specific fashion. GRIP/ABP family proteins contain six to seven PDZ domains and interact via its sixth PDZ domain (class Ⅱ) with the C-termini of various proteins, including liprin-α. In addition the PDZ456 domain mediates the formation of homo- and heteromultimers of GRIP proteins. To better understand the structural basis of peptide recognition by a class Ⅱ PDZ domain and DZ-mediated multimerization, we determined the crystal structures of the GRIPI PDZ6 domain, alone and in complex with a synthetic C-terminal octapeptide of human liprin-α, at resolutions of 1.5 Å and 1.8 Å, respectively. Remarkably, unlike other class Ⅱ PDZ domains, Ile736 at αB5 rather than conserved Leu732 at αB1 makes a direct hydrophobic contact with the side chain of the Tyr at the -2 position of the ligand. Moreover, the peptide-bound structure of PDZ6 shows a slight reorientation of helix αB, indicating that the second hydrophobic pocket undergoes a conformational adaptation to accommodate the bulkiness of the Tyr's side chain, and forms an antiparallel dimer through an interface located at a site distal to the peptide-binding groove. This configuration may enable formation of GRIP multimers and efficient clustering of GRIP-binding proteins.

• 복식문화연구
• /
• 제19권3호
• /
• pp.488-500
• /
• 2011

[ $D{\'{e}}formation$ ]is one of the most important cultural factor which puts people at ease and gives a sense of security. This study, in the process, analyzes the works of Moschino. Moschino's designs are rated to have approached the sublime when it comes to transforming the psychological anxiety of everyday living into a laughter. After selecting one hundred-two of Moschino's designs from the Internet Web site(www.cft.or.kr, www.samsungdesign.net), this study examines and analyzes the characteristics and types of deformation found in them. The result as follows. The examination of deformation found in Moschino designs can be classified into a transformation, distortion, exaggeration, and illusion. Transformation, a conscious change of the existing form or function, was shown as the change of an existing position, form, function and designation of a new function. Distortion, an interpretation away from the reality or a "wrong interpretation," was shown by placing opposing factors in left-right position as an extreme asymmetry. Exaggeration, always beyond the realm of reality, was shown thorough an enlargement or a magnification of a specific part and a repetition of a detail factors. Illusion, through a distortion of reality results in something that looks new, was expressed through the effects of wearing a two-pieces, an expression of details, effects of wearing accessories, and an expression of a dynamism. Therefore, Moschino has reflected the desire of homo modern to transform the existing situation through many techniques of deformation.