• Korean Journal of Veterinary Service
• /
• v.30 no.1
• /
• pp.145-154
• /
• 2007

In Korea, it is the one of controversial problems to distinguish heifer from cow in slaughtered Holstein cattle. This study was conducted to evaluate the several criterions which could be used to discriminate heifer from cow. Some criterions have shown significant differences between heifer and cow in ante and post-mortem inspections(p<0.01). Firstly, the numbers of milk teeth of heifer and cow were $6.12{\pm}1.92$ ($Mean{\pm}Standard$ deviation) and $0.03{\pm}0.39$ respectively. The teat diameter(D) and length(L) of cow were $26.43{\pm}4.31mm$ and $47.76{\pm}6.89mm$ respectively. However, those of heifer were significantly smaller (D: $18.04{\pm}5.04mm$, L: $28.61{\pm}8.91mm$) than those of cow. The size of udder was $203.68{\pm}16.84mm$ in Holstein cow and $112.70{\pm}20.59mm$ in heifer. Secondly, the uterus size of cow was significantly bigger than that of heifer and caruncle in mucosa of uterus could be easy to be confirmed by necropsy inspection. It was also obvious that the folding and length of uterus body were significantly remarkable in cow. Lastly, the pelvic cavity was $196.33{\pm}10.01mm$ in heifer and $220.90{\pm}11.41mm$ in cow. The ossifying maturation of heifer was $2.64{\pm}0.82$ and $6.71{\pm}1.81$ in cow. As the results, this study can be helpful for meat inspectors to discriminate the non-delivery heifer from delivery cow in Holstein cattle.

• Journal of Veterinary Clinics
• /
• v.29 no.4
• /
• pp.348-351
• /
• 2012

A vaginal speculum examination of a 17-month-old Holstein heifer with a history of repeated artificial insemination (AI) failures via the cranial vagina revealed the presence of an intact hymen (imperforate hymen) beyond the external urethral orifice, which resembled the vaginal wall. An ultrasonographic examination detected hypoechoic fluid within the vaginal cavity between the intact hymen and the cervix, indicating the presence of hydrocolpos. After sedation and epidural anesthesia, the intact hymen was incised using a scalpel and scissors and removed, while the incised portions were sutured using a simple interrupted method. The heifer became pregnant after three consecutive estruses with the intervals of approximately 20 days and concomitant AIs. The heifer gave birth to a healthy male calf with no complications after a 291-day gestation period. This case reports the presence of an imperforate hymen with hydrocolpos in a Holstein heifer, followed by successful insemination, conception and delivery after removal of the imperforate hymen.

• Korean Journal of Veterinary Service
• /
• v.43 no.4
• /
• pp.245-250
• /
• 2020

Infectious bovine keratoconjunctivitis (IBK) is the most common ocular disease in cattle, manifesting as corneal opacity, corneal ulcerations and potentially vision loss. The present report describes a 10-month-old Holstein Friesian heifer with IBK treated by systemic tulathromycin, and subconjunctival injection of penicillin and dexamethasone. We investigated changes in the hematological indices and microorganisms related to IBK after treatment. Neutrophils and monocytes decreased during recovery, so it was assumed that these two types of white cells are associated with IBK. Moraxella bovoculi was cleared in the eye, nasal cavity, and oral cavity after treatment. The distribution of M. bovoculi before treatment indicated that a combined systemic and subconjunctival treatment was necessary. The lesioned eye was found to be overwhelmed by Mycoplasma bovoculi, while pathogen abundance was reduced in the nasal cavity and oral cavities. These results suggest that antibiotic treatment can alter the composition and relative abundance of microorganisms.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.17 no.3
• /
• pp.213-222
• /
• 1997

This study was carried out to investigate the reproduction efficiency and disease of Holstein heifer grazed on mixed pasture desighed with association of tall fescue and white clover cultivar (3 treatments = TI : Tall fescue Fawn + White clover Regal + Orchardgrass + Perennial ryegrass, T2 : Tall fescue Roa + Ladino clover Regal + Orchardgrass + Perennial ryegrass, T3 : Tall fescue Roa + White clover Tahora + Orchardgrass + Perennial ryegrass) on reproduction efficiency and disease in Holstein heifers, ftom April 1994 to November 1996. The results are as follows: 1. Erythrocyte counts, hemoglobin and packed cell volume of hematological values were $8.12\times10^6/{\mu}\ell, 9.9g/m\ell and 30.6m\ell/100m\ell$ respectively. While those were decreased to $4.84~5.93 \times10^6/{\mu}\ell, 7.0~7.8g/100m\ell and 22.8~28.3m\ell/100m\ell$, on pastuage (May-August), WBC counts was increased from $14.4\times10^3/{\mu}\ell to 14.0~18.3 \times 10^{3/\mu\ell}$ on pasturage. No significant differences were detected among the treatments on pastures. 2. Parasitemia levels increased to $14.0\pm8.2$(parasite/1,000rbc) at 1 month after pasturage. 3. At the occurrence of tirst estrus and conception, the average age of the Holstein heifers was $14.3\pm1.9$ and $17.4\pm1.5$ months, respectively, and the average interval of estrus cycle was $20.6\pm1.0$ days. Howerer, there was no significant difference on reproductive efficiency among 3 treatments. 4. The outbreak of diseases were tick bane disease, pink eye, pneumonia, mastitis, gastroentitis, waterpoisoning ringworm papillon and atosis during pasturage period(may-october). These diseases were showed no significant difference among 3 treatments. 5. In conclusion, there are no significant difference on the reproductive effeciency and disease outbreak among three treatments.

• Journal of Embryo Transfer
• /
• v.23 no.4
• /
• pp.257-261
• /
• 2008

The objective of present study was to investigate the effect of seasons on reproductive performance of Hanwoo and Holstein heifers. Heat stress in summer or cold stress in winter stress to Hanwoo and Holstein heifers may bring reproduction failure, which would pose an important economic loss, even around Daegwallyeong region located in high mountainous area. Seasonal differences in the serum levels of LH, FSH and progesterone ($P_4$) in response to environmental factors (hot and cold) out of 20 pubertal Hanwoo heifers in Daegwallyeong, Gangwon Province and 20 non-lactating Holstein heifers in Chonan city of Republic of Korea at 2-3 years of age were compared. Blood samples for hormonal analysis were from jugular vein after detection of estrus repeatedly over four seasons within four-week intervals (Spring: May to June, Summer: July to August, Autumn: October to November and Winter: January to February). In Hanwoo heifer population, averages of LH and FSH concentration in spring and in summer were greater compared to those in winter (p<0.05). LH or FSH levels tended to be greater (p=0.06) in spring and less (p=0.09) in winter compared to the levels in autumn. Only in summer, cattle seemed to show lower LH or FSH secretion (p<0.05). Similar to the results in Hanwoo heifers, the serum concentrations of LH and FSH in Holstein heifers decreased further by heat stress in summer when P 4 levels were high during luteal phase. The results demonstrate significant effect of summer heat on reproduction of Hanwoo or Holstein heifers. Although parameters indicating the extent of heat stress were not measured in this study, we suggest that serum hormone levels could be considered as successful indicators of summer heat stress condition for Hanwoo and Holstein heifers even under rather cool summer climate.

• Korean Journal of Agricultural Science
• /
• v.46 no.1
• /
• pp.27-31
• /
• 2019

It is well documented that intensive selection in dairy cattle for economic value such as increased milk yield led to a decline in reproductive performance. Recent studies using genome-wide association studies (GWASs) discovered candidate genes involved in the lower fertility including embryo development and conception rates. However, the information, which showed a lower reproductive performance, is limited to dairy cattle, especially Holstein, and the candidate genes were not examined in the Korean native cattle Hanwoo which has been intensively selected and bred for meat in the last few decades. We selected the candidate genes WBP1 and PARM1 reported to be associated with cow and/or heifer conception in dairy cattle and analyzed the genotype because those genes have non-synonymous single nucleotide polymorphisms (SNPs). To determine the single base change, we used the high resolution melting (HRM) assay which is rapid and cost-effective for a small number of genes. We found that most heifers with higher conception (1: service per conception) have the AA genotype coding Threonine rather than Proline in the WBP1 gene. We did not detect an association for a SNP in PARM1 in our analysis. In conclusion, the genetic variation of WBP1 can be used as a selective marker gene to improve reproductive performance, and HRM assay can be used to identify common SNP genotypes rapidly and cost effectively.

• Korean Journal of Veterinary Research
• /
• v.15 no.2
• /
• pp.181-185
• /
• 1975

The experiment was carried out to determine the concentrations of total calcium and inorganic phosphorus of thirty rera obtained from pregnant Holitein cows, six sera from normal non-pregnant cows with one month after delivery, six sera from cows with ovarian cyst and six sera from heifer of sixteen months old. The changes of total calcium and inorganic phosphorus were observed on the 2nd, 4th, 6th, 8th, and l0th month of gestation. The results obtained in this work were summarized as follows: 1. The mean values of total serum calcium obtained from the pregnant group were $9.59{\pm}0.34mg/100ml$, from the non-pregnant group $10.23{\pm}0.47mg/100ml$, from the infertility grou $10.37{\pm}0.27mg/100ml$, and from the heifer group $10.07{\pm}0.20mg/ml$. The change of total serum calcium values in the pregnant group revealed the tendency of decrease as gestation progresses and the lowest value appeared on the sixth month. The mean value of total serum calcium in the pregnant group was lower than those of the other groups, but the differences were not statistically significant. 2. The mean values of serum inorganic phosphorus obtained from the pregnant group were $5.82{\pm}0.31mg/100ml$, from the non-pregnant group $5.92{\pm}0.44mg/100ml$, from the infertility group $5.80{\pm}0.49mg/100ml$, and from the heifer group $5.10{\pm}0.32mg/100ml$. The change during pregnancy showed a slight fall and the lowest value appeared on the sixth month. The differences of serum inorganic phosphorus values between the pregnant group and the other adult groups were not significant, but these adult groups showed a significantly higher value than that of heifer group (p<0.05).

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.36 no.4
• /
• pp.381-386
• /
• 2016

The objective of this research was to evaluate the effects of two different feeding systems on blood metabolites in Holstein heifers and analyze the economic impacts of the feeding systems. The following two experiments were conducted to investigate the effects of feeding system on blood metabolite changes in Holstein heifers and analyze the economic impacts of the two systems. In experiment 1, the effects of two different feeding systems on cortisol, progesterone, and estradiol in Holstein heifers were examined. In experiment 2, the effects of two different feeding systems on the body weights of Holstein heifers and profitability of the two feeding systems were studied. Results showed that the pasture-raised heifers showed significantly decrease in the levels of blood cortisol (p<0.05) and increases in the levels of progesterone and estradiol (p>0.05) when compared with heifers raised in indoor feeding system. The average daily gain was significantly higher (p<0.05) in indoor-raised heifers (0.73 kg/day) as compared to pasture-raised heifers (0.58 kg/day). Also, 25.2% more profits were obtained from the pasture feeding system as compared to the indoor feeding system. These results together would be useful in the investigation of feeding system and growth performance in dairy cattle.

• Asian-Australasian Journal of Animal Sciences
• /
• v.6 no.1
• /
• pp.91-97
• /
• 1993

This experiment was carried out to examine the effects of urea-treated rice straw feeding on the growth performance of crossbred Holstein heifers under different feeding conditions. In the first experiment, the animals were given diets having 2 levels of TDN and CP and 3 levels of crude fiber (22, 30 and 36%) which were formulated with urea-treated rice straw and concentrates. Daily weight gain of heifers was not different between 22% and 30% CF diets, but the reduction of TDN or CP level to 90% of the requirements decreased the weight gain. Fiber content of 36% also reduced the body weight gain. The reduction of TDN significantly reduced DM intake and increased feed conversion ratio. Feed cost per kg weight gain was significantly increased by an increase in CF to 36%. In the second experiment, separate feeding and total mixing feeding were compared. There were no significant differences between the two feeding systems in body weight gain although the possibility of superiority in SF to TMF remained. DM intake was not affected by the feeding system, but 30% CF diet gave higher DM intake. Feed cost per kg weight gain was lower in the 30% CF diet.

• Asian-Australasian Journal of Animal Sciences
• /
• v.4 no.4
• /
• pp.341-351
• /
• 1991

Sixteen Holstein heifer calves were used in an 112-day trial to study the effects of probiotic supplementation on growth performance and rumen metabolism. Calves were divided into four groups of four calves each, with two groups receiving the probiotic supplement and two groups serving as controls. Calves were limited to 1.6 kg dry matter of a corn-barley based grain mix per day. Long-stem bromegrass hay was fed as forage the first 56 days and bromegrass silage the last 56 days of the trial. Probiotic (28 g/d/calf) was fed along with the grain mix twice daily. Data were analyzed for the entire trial and also for the separate hay and silage feeding periods. Total weight gain and average daily gain were not affected (p>.05) by probiotic supplementation. Dry matter intake was lower (p<.05) and feed efficiency (kg feed/kg weight gain) was improved slightly during the hay feeding period for the probiotic-supplemented calves. Wither height gain was greater (p<.05) during the hay period and lower (p<.05) during the silage period for probiotic-supplemented calves. Heart girth gain was improved (p<.07) by probiotic supplementation, particularly during the hay feeding period (p<.05). Total rumen volatile fatty acid (VFA) concentration was higher (p<.05) with the probiotic-supplemented calves. Molar proportions of individual VFA were not affected (p>.05). Rumen ammonia-N and plasma urea-N concentration were lower (p<.05) for probiotic-supplemented calves during the hay feeding period. Total tract nutrient digestibility was not affected (p>.05). Some improvements in animal performance and changes in rumen and blood metabolites were observed when calves were supplemented with probiotic. Effects due to probiotic supplementation were most pronounced during the hay feeding period.