• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.5
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• pp.114-120
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• 2002

In a distributed real-time control system, it is essential to confirm the timing behavior of all tasks because these tasks of each real-time controller have to finish their processes within the specified time intervals called a deadline. In order to satisfy this objective, the timing analysis of a distributed real-time system such as shcedulability test must be performed during the system design phase. In this study, a simple application of CAN fur a vehicle body network system is formulated to apply to a holistic scheduling analysis, and the worst-case execution time (WCET) and the worst-case end-to-end response time (WCRT) are evaluated in the point of holistic system view.

• KIEAE Journal
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• v.10 no.1
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• pp.97-107
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• 2010

Although aspiration towards quality of life and holistic health has been growing faster in our modern society and the affordance of health in built environment has been more acknowledged, there has been hardly any development on built environment planning and design theory that can be comfortably and confidently used in creating built environment to promote holistic health. Thereby, this study sets out to experimently formulate a composite theory that explains the relationship between health and built environment. The main methodology of this study is literature review and analysis. Theories that have been applied in other similar fields were chosen to be analyzed by health related perspectives and graft those theories onto holistic health viewpoints to compose a comprehensive theory. Selected theories that were considered useful to be analyzed were Lawton's Environment Press Theory, Carp & Carp's Complementary & Congruence Theory, Valins' Activity-based Design Criteria Theory, Atchley's Continuity Theory, Murtha & Lee's User Benefit Criteria Theory, and Alexander's Pattern Language Theory. Characteristics of these theories were compared by their abstractness and concreteness, and the range of application, and analyzed by a holistic health perspective. Then, these theories were comprehensively structuralized and synthesized as a built environment for health theory. This study has its significance in providing a base to develop healthy built environment research further as it introduced a conceptual framework which explains spatial elements in the health functionality point of view.

• Journal of Ginseng Research
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• v.46 no.6
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• pp.750-758
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• 2022

Background: Mild cognitive impairment (MCI) is a transitional condition between normality and dementia. Ginseng is known to have effects on attenuating cognitive deficits in neurogenerative diseases. Ginsenosides are the main bioactive component of ginseng, and their protein targets have not been fully understood. Furthermore, no thorough analysis is reported in ginsenoside-related protein targets in MCI. Methods: The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry (LC-MS) analysis. Network pharmacology approach was used to collect the therapeutic targets for MCI. Based on the above-mentioned overlapping targets, we built up a proteineprotein interaction (PPI) network in STRING database and conducted gene ontology (GO) enrichment analysis. Finally, we assessed the effects of ginseng total saponins (GTS) and different ginsenosides on mitochondrial function by measuring the activity of the mitochondrial respiratory chain complex and performing molecular docking. Results: We screened 2526 MCI-related protein targets by databases and 349 ginsenoside-related protein targets by DARTS. On the basis of these 81 overlapping genes, enrichment analysis showed the mitochondria played an important role in GTS-mediated MCI pharmacological process. Mitochondrial function analysis showed GTS, protopanaxatriol (PPT), and Rd increased the activities of complex I in a dose-dependent manner. Molecular docking also predicted the docking pockets between PPT or Rd and mitochondrial respiratory chain complex I. Conclusion: This study indicated that ginsenosides might alleviate MCI by targeting respiratory chain complex I and regulating mitochondrial function, supporting ginseng's therapeutic application in cognitive deficits.

• Journal of Korea Multimedia Society
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• v.18 no.7
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• pp.834-842
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• 2015

We propose a method to construct composite feature vector based on discriminant analysis for face recognition. For this, we first extract the holistic- and local-features from whole face images and local images, which consist of the discriminant pixels, by using a discriminant feature extraction method. In order to utilize both advantages of holistic- and local-features, we evaluate the amount of the discriminative information in each feature and then construct a composite feature vector with only the features that contain a large amount of discriminative information. The experimental results for the FERET, CMU-PIE and Yale B databases show that the proposed composite feature vector has improvement of face recognition performance.

• Journal of Ginseng Research
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• v.45 no.4
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• pp.465-472
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• 2021

Background: Ginseng can help regulate brain excitability, promote learning and memory, and resist cerebral ischemia in the central nervous system. Ginsenosides are the major effective compounds of Ginseng, but their protein targets in the brain have not been determined. Methods: We screened proteins that interact with the main components of ginseng (ginsenosides) by affinity chromatography and identified the 14-3-3 ζ protein as a potential target of ginsenosides in brain tissues. Results: Biolayer interferometry (BLI) analysis showed that 20(S)-protopanaxadiol (PPD), a ginseng saponin metabolite, exhibited the highest direct interaction to the 14-3-3 ζ protein. Subsequently, BLI kinetics analysis and isothermal titration calorimetry (ITC) assay showed that PPD specifically bound to the 14-3-3 ζ protein. The cocrystal structure of the 14-3-3 ζ protein-PPD complex showed that the main interactions occurred between the residues R56, R127, and Y128 of the 14-3-3 ζ protein and a portion of PPD. Moreover, mutating any of the above residues resulted in a significant decrease of affinity between PPD and the 14-3-3 ζ protein. Conclusion: Our results indicate the 14-3-3 ζ protein is the target of PPD, a ginsenoside metabolite. Crystallographic and mutagenesis studies suggest a direct interaction between PPD and the 14-3-3 ζ protein. This finding can help in the development of small-molecular compounds that bind to the 14-3-3 ζ protein on the basis of the structure of dammarane-type triterpenoid.

• Journal of Ginseng Research
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• v.47 no.5
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• pp.662-671
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• 2023

Background: 20(S)-protopanaxadiol (PPD), a ginsenoside metabolite, has prominent benefits for the central nervous system, especially in improving learning and memory. However, its transcriptional targets in brain tissue remain unknown. Methods: In this study, we first used mass spectrometry-based drug affinity responsive target stability (DARTS) to identify the potential proteins of ginsenosides and intersected them with the transcription factor library. Second, the transcription factor PURA was confirmed as a target of PPD by biolayer interferometry (BLI) and molecular docking. Next, the effect of PPD on the transcriptional levels of target genes of PURA in brain tissues was determined by qRT-PCR. Finally, bioinformatics analysis was used to analyze the potential biological features of these target proteins. Results: The results showed three overlapping transcription factors between the proteomics of DARTS and transcription factor library. BLI analysis further showed that PPD had a higher direct interaction with PURA than parent ginsenosides. Subsequently, BLI kinetic analysis, molecular docking, and mutations in key amino acids of PURA indicated that PPD specifically bound to PURA. The results of qRT-PCR showed that PPD could increase the transcription levels of PURA target genes in brain. Finally, bioinformatics analysis showed that these target proteins were involved in learning and memory function. Conclusion: The above-mentioned findings indicate that PURA is a transcription target of PPD in brain, and PPD upregulate the transcription levels of target genes related to cognitive dysfunction by binding PURA, which could provide a chemical and biological basis for the study of treating cognitive impairment by targeting PURA.

• Journal of Ginseng Research
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• v.46 no.5
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• pp.666-674
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• 2022

Background: Ginsenosides and their metabolites have antidepressant-like effects, but the underlying mechanisms remain unclear. We previously identified 14-3-3 ζ as one of the target proteins of 20 (S)-protopanaxadiol (PPD), a fully deglycosylated ginsenoside metabolite. Methods: Corticosterone (CORT) was administered repeatedly to induce the depression model, and PPD was given concurrently. The tail suspension test (TST) and the forced swimming test (FST) were used for behavioral evaluation. All mice were sacrificed. Golgi-cox staining, GSK 3β activity assay, and Western blot analysis were performed. In vitro, the kinetic binding analysis with the Biolayer Interferometry (BLI) was used to determine the molecular interactions. Results: TST and FST both revealed that PPD reversed CORT-induced behavioral deficits. PPD also ameliorated the CORT-induced expression alterations of hippocampal Ser9 phosphorylated glycogen synthase kinase 3β (p-Ser9 GSK 3β), Ser133 phosphorylated cAMP response element-binding protein (p-Ser133 CREB), and brain-derived neurotrophic factor (BDNF). Moreover, PPD attenuated the CORT-induced increase in GSK 3β activity and decrease in dendritic spine density in the hippocampus. In vitro, 14-3-3 ζ protein specifically bound to p-Ser9 GSK 3β polypeptide. PPD promoted the binding and subsequently decreased GSK 3β activity. Conclusion: These findings demonstrated the antidepressant-like effects of PPD on the CORT-induced mouse depression model and indicated a possible target-based mechanism. The combination of PPD with the 14-3-3 ζ protein may promote the binding of 14-3-3 ζ to p-GSK 3β (Ser9) and enhance the inhibition of Ser9 phosphorylation on GSK 3β kinase activity, thereby activating the plasticity-related CREBeBDNF signaling pathway.

• Journal of the Korean housing association
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• v.22 no.2
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• pp.101-109
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• 2011

Until now, several comparative approaches were developed within the studies of Korean, Chinese, and Japanese traditional housings. In those studies, however, each space in the traditional houses was only treated in individual and fragmentary manners, and they lacked the interpretation of the topological attribute of each space within a holistic structure organized by unit spaces, and of the cultural-behavioral meaning of them within a holistic space-use pattern of the housing. The topological attribute and behavioral meaning can be analyzed and interpreted with the quantitative spatial analysis method such as Space Syntax. This study aims to analyze the traditional housings in Korea, China and Japan in the holistic aspect of spatial structure using Space Syntax, and to compare the analysis results with relating the structural attributes to the space-use pattern. In this study, the 'Banga' in Chosun era, the 'Siheyuan' in Ming-Ching era, and the 'Shoinzukuri' in Edo era were selected as the analysis subjects. The integration indices were calculated from the convex maps representing the subjects, and the common and different attributes of the three subjects were defined through comparative analyses.

• Research in Community and Public Health Nursing
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• v.27 no.1
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• pp.60-71
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• 2016

Purpose: This study was to present education and holistic care of Elizabeth J. Shepping (1880~1934), a nursing missionary and a founder of the Chosun Nursing Association who visited Korea during the Japanese colonial period. Methods: Primary and secondary sources were collected and analyzed. Results: This study provides important implications regarding Shepping's holistic nursing as follows: First, she came to Korea after studying nursing and bibliology and being trained for nursing missionary works. Second, she cared for many Koreans, especially Korean women, to protect them from poverty, oppression, ignorance, and illnesses. Third, she continued to spread holistic care in hospitals and other local communities. She trained nurses, developed nursing education, and produced a large number of domestic nursing leaders by establishing women's Bible school. Fourth, she founded the Chosun Nursing Association, serving as its first president for 10 years and applied to join the International Council of Nurses (ICN). Conclusion: Finally, suggestions were provided for future research, and it will be necessary to study thoroughly nursing achievements by nurses from other countries who practiced their nursing activities in Korea, and such studies are expected to lead to analysis of nursing missionaries' experiences.

• Journal of the Korean housing association
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• v.21 no.2
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• pp.77-86
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• 2010

Korean population is now aging at an unprecedented rate. Fertility decline together with rapid improvements in life expectancy is the dominant demographic trend. As human life and housing environment are inseparably related to each other, the importance of conditions of housing environment for active aging among seniors has been received considerable attention as vital ones to expand the independency period of seniors. The purpose of this case study is to identify relationships between environmental affordance and perceived holistic health of the active elderly living in a retirement community located in Seoul. With spending time personally in the community to observe their lives, the researcher could be in close rapport with the residents. After the period of getting familiar with each other, in-depth individual interview and focus group interview were conducted and then, qualitative analysis was completed. As a result, general evidences showed that perceived seniors' health on the whole was improved physically, socially, and mentally by moving into the community providing appropriate environment affordance with the older adults. The study presents that retirement relocation into an improper environment may be a negative challenge, however, it may positively influence on the health and well-being of those who live there if the environment properly supports seniors‘ needs physically and socially