• Journal of Life Science
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• v.22 no.2
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• pp.259-265
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• 2012

A bacterial strain, CK214, exhibiting high motility on an LB agar (1.5%, w/v) surface was isolated from the environment. The formation of unusual agar shrinking around colonies on agar plates was observed. The strain grew on minimal media containing pure agar as a sole carbon source. The cell-free culture supernatant of CK214 generated a reduced form of sugar in the in vitro reaction with the use of pure agar as a substrate, suggesting the secretion of an agar-degrading enzyme. The CK214 strain showed swarming motility on the solid media containing a wide range of concentrations of agar (0.5, 1.0, 1.5, 2.0% w/v). Various tests, including Gram staining, API analysis, and phylogenetic analysis based on 16S rDNA sequences identified that the CK214 strain was a G(+) rod-shaped bacterium grouped in genus Paenibacillus. Electron microscopic analysis demonstrated that the P. CK214 strain is peritrichously flagellated. Through transposon random mutagenesis, several agar-degrading activity defective mutants (ADMs) were generated. These mutants will be used in the future experimentation for the study of the correlation between agar-degrading activity and motility.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.2 no.2 s.3
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• pp.43-54
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• 2003

As the demand for ubiquitous mobile wireless Internet grows, vehicles are receiving a lot of attention as new networking platforms. The demand for 4G all-IP networks encourages vehicle networks to be connected using IPv6. By means of network mobility (NEMO) support, we can connect sensors, controllers, local ,servers as well as passengers' devices of a vehicle to the Internet through a mobile router. The mobile router provides the connectivity to the Internet and mobility transparency for the rest of the mobile nodes of an in-vehicle nv6 network. So, it is .important for the mobile router to assure reliable connection and a sufficient data rate for the group of nodes behind it. To provide reliability, this paper proposes an adaptive multihoming architecture of multiple mobile routers. Proposed architecture makes use of different mobility characteristics of different vehicles. Simulation results with different configurations show that the proposed architecture increases session preservation thus increases reliability and reduces packet loss. We also show that the proposed architecture is adaptive to heterogeneous access environment which provide different access coverage areas and data rates. The result shows that our architecture achieves sufficient data rates as well as session preservation.

• Journal of the Korean Institute of Intelligent Systems
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• v.22 no.2
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• pp.192-197
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• 2012

With the advent of DNA microarray technologies, researches for disease diagnosis has been actively in progress. In typical experiments using microarray data, problems such as the large number of genes and the relatively small number of samples, the inherent measurement noise and the heterogeneity across different samples are the cause of the performance decrease. To overcome these problems, a new method using functional modules (e.g. signaling pathways) used as markers was proposed. They use the method using an activity of pathway summarizing values of a member gene's expression values. It showed better classification performance than the existing methods based on individual genes. The activity calculation, however, used in the method has some drawbacks such as a correlation between individual genes and each phenotype is ignored and characteristics of individual genes are removed. In this paper, we propose a method based on the ensemble classifier. It makes weak classifiers based on feature vectors using subsets of genes in selected pathways, and then infers the final classification result by combining the results of each weak classifier. In this process, we improved the performance by minimize the search space through a filtering process using gene-gene interaction information and the mRMR filter. We applied the proposed method to a classifying the lung cancer, it showed competitive classification performance compared to existing methods.

• Geophysics and Geophysical Exploration
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• v.11 no.2
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• pp.148-152
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• 2008

Multichannel seismic profiles reveal a strong bottom simulating reflector (BSR) occurring below the seafloor in the plain of the Ulleung Basin, East Sea (Japan Sea). The essential characteristics of the BSR include its cross-cutting relationship to strata, strong amplitude, and reverse polarity with respect to the seafloor reflection, representing the base of the gas hydrate stability zone (BHSZ). The BSR reflection coefficient ranging from -0.23 to -0.26 is 1.5${\sim}$1.7 times that of the seafloor reflection and interval velocities decrease to less than 700 m/s below the BSR. These features indicate the existence of free gas beneath the GHSZ. Heat flow, estimated from the BSR depth as $95{\sim}98mW/m^2$, is in good agreement with measured values. Therefore, the BSR can be efficiently used to estimate regional distribution of heat flow in the Ulleung Basin.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.3
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• pp.157-168
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• 2014

From a complex systems perspective, ecohydrological systems in forests may be characterized with (1) large networks of components which give rise to complex collective behaviors, (2) sophisticated information processing, and (3) adaptation through self-organization and learning processes. In order to demonstrate such characteristics, we applied the recently proposed 'process networks' approach to a temperate deciduous forest in Gwangneung National Arboretum in Korea. The process network analysis clearly delineated the forest ecohydrological systems as the hierarchical networks of information flows and feedback loops with various time scales among different variables. Several subsystems were identified such as synoptic subsystem (SS), atmospheric boundary layer subsystem (ABLS), biophysical subsystem (BPS), and biophysicochemical subsystem (BPCS). These subsystems were assembled/disassembled through the couplings/decouplings of feedback loops to form/deform newly aggregated subsystems (e.g., regional subsystem) - an evidence for self-organizing processes of a complex system. Our results imply that, despite natural and human disturbances, ecosystems grow and develop through self-organization while maintaining dynamic equilibrium, thereby continuously adapting to environmental changes. Ecosystem integrity is preserved when the system's self-organizing processes are preserved, something that happens naturally if we maintain the context for self-organization. From this perspective, the process networks approach makes sense.

• Journal of KIISE:Databases
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• v.32 no.1
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• pp.56-70
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• 2005

The results of location-dependent queries(LDQ) generally depend on the current locations of query issuers. Many mechanisms, e.g. broadcast scheme, hoarding, or racking policy, have been developed to improve the system peformance and provide better services, which are specialized for LDQs. Considering geographical adjacency of data and characteristics oi target area, caching policy and broadcast scheme affect the overall performance in LDQ. For this reason, we propose both the caching policy and broadcast scheme, which these features are reflected in. Based on the adjacency of data in LDQ, our broadcast scheme use Hilbert curve to cluster data. Moreover, in order to develop the caching policy suitable for LDQ on urban area, we apply the moving distance of a MH(Mobile Host) to our caching policy We evaluate the performance of the caching policy measuring the workload of MHs and the correctness of LDQ results and the performance of the broadcast scheme measuring the average setup-time of MHs in our experiments. Finally, we expect that our caching policy Provides more correct answers when executing LDQ in focal cache and leads significant improvement of the performance of MHs. It also seems quite probable that our broadcast scheme leads improvement of battery life of the MH.

• Korean Journal of Microbiology
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• v.34 no.4
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• pp.212-218
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• 1998

An obligately methylotrophic bacterium which produces extracellular polysaccharide (EPS) was isolated through methanol-enrichment culture technique. The isolate was aerobic, nonmotile, and gram negative rod and exibited catalase, but no oxidase, activity. Plasmid, carotenoid, and poly-${\beta}$-hydroxybutyric acid were not found. The guanine plus cytosine content of DNA was 52-56%. The isolate was found to grow only on methanol and monomethylamine. Growth was optimal ($t_d=2.4h$) at $35^{\circ}C$ and pH 6.5 in a mineral medium containing 0.5% (v/v) methanol, 25 mM phosphate, and 0.212% ammonium sulfate. Methanol was assimilated through the ribulose monophosphate pathway. Maximun amount of EPS was produced in cells growing at the mid-stationary growth phase at $30^{\circ}C$ in a mineral medium (PH 6.5) containing 1.0% (v/v) methanol in the CIN ratio of 54.7. Thin-layer chromatographic and high performance liquid chromatographic analysis revealed that the EPS was composed of glucose and galactose. EPS which was not treated with ethanol (Pbe) exhibited stable viscosity under various concentrations of salts and temperatures hut showed high viscosity at low pH. EPS precipitated with ethanol (Pae) was found to be more stable in viscosity than the Pbe at various salt concentrations, temperatures, and pH. The Pae also exhibited higher viscosity than the Pbe and xanthan gum. Scanning electron microscopy revealed that the lyophilized Pbe and Pae have a multi-layered structure and a structure of thick fibers, respectively.

• Journal of the Korean Wood Science and Technology
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• v.30 no.2
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• pp.144-150
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• 2002

Indian mallow is characterized by the rapid growth and high harvest of the fibrous materials. This study was carried out to investigate the sheet properties of Hanji using Indian mallow, made by different pulping methods, such as alkali and sulfomethylated pulpings and different stock compositions, various mixing ratios of bast fiber, woody core and whole stalk fibers. The results from this study were summarized as follows. The length and width of the bast fibers were 1.40-430 mm(av. 235 mm), and 9.2-26.4 ㎛(av.18.3 ㎛), respectively. The cell wall thickness was 4.0-115 ㎛(av. 7.7 ㎛). Runkel ratio, flexibility coefficient and fiber length/fiber width ratio were 1.38, 0.42 and 128 respectively. Bast fiber and whole stalk were cooked by alkali and sulfomethylated methods. Sulfomethylated pulping resulted in superior pulp in terms of yield and quality as compared with those of alkali pulping. The pulp yields of bast fiber was higher than those of whole stalk

• Tuberculosis and Respiratory Diseases
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• v.41 no.5
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• pp.537-545
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• 1994

Background: In 1987, the British Thoracic Society (BTS) subjected an extensive list of patient variables to statistical analysis in a prospective study of prognosis in 453 adults with community-acquired pneumonia and, subsequently published guidelines for management of severe community acquired pneumonia. It was hoped that those at risk of dying from community acquired pneumonia could be easily identified and treated appropriately, thereby reducing mortality. To date, severe community acquired pneumonia has not been well studied in Korea. Therefore, we studied retrospectively 10 patients dying of severe community acquired pneumonia in Dongsan Hospital to see clinical manifestations of dying of severe community-acquired pneumonia. Methods: Between July 1987 and july 1993, 498 patients were admitted to Keimyung University Dongsan Hospital with community acquired pneumonia, and 77 of them received intensive care. Of the 77 patients, 10 patients died. We reviewed medical records of these patients. Results: 1) The mean age of the patients was 56.2 years(range, 25 to 75 years). There were 7 men and 3 women. Seven patients(70%) were older than 60years of age. 2) The clinical features on admission were as follows: tachypnca, hypoxemia, mental change, cyanosis, leukopenia, leukocytosis, azotemia, hypotension, hypoalbuminemia in order of frequency. Three patients had one abnormal physical finding, 3 patients had 2, 2 patients had 3, and 2 patients had none of these abnormal physical findings. All patients had at least one of the abnormal laboratory findings. 3) A potential bacterial pathogen was isolated in sputum culture from 2 patients. One was E.coli, the other Enterobacter species. Sputum stain were positive in eight cases (G(+)cocci in six, G(+) cocci and G(-)bacilli in two). 4) Features of respiratory failure were the main reasons for ICU transfer, but two patients were transferred only following a cardiac or respiratory arrest in the general ward. 5) The mean of 2.7 different antibiotics were given to the patients. The aminoglycoside and first generation cephalosporin were the most frequently prescribed antibiotics, followed by the third generation cephalosporin and vancomycin. The most frequently prescribed antibiotics combination was a 1st generation cephalosporin plus an aminoglycoside. 6) Seven patients death(70%) occured after admission within the first five days, and a mean duration of hospitaliztion was 11.2 days. Conclusion: As the results show most death occured within the first five days after admission and aged patients; consequently, an aggressive intensive treatment should be provided early to the patients with severe community acquired pneumonia, and we should pay more attention to the aged patients.

• Journal of Environmental Impact Assessment
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• v.24 no.6
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• pp.578-592
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• 2015

To investigate characteristics and seasonal variations of carbonaceous species for $PM_{2.5}$ in Seoul metropolitan area, Korea, we measured organic carbon (OC) and elemental carbon (EC) from January 2014 to December 2014 using a semi-continuous OC/EC Analyzer (Model-4, Sunset Lab.). Mean concentrations of OC and EC were estimated $4.1{\pm}2.7{\mu}g/m^3$ and $1.6{\pm}1.0{\mu}g/m^3$, respectively. The annual averaged OC/EC ratio was $2.9{\pm}2.7$. Concentrations of OC and EC comprised 13% and 5% of $PM_{2.5}$ and the mass fraction of both was the highest in fall. OC and EC showed similar trend in seasonal variations. Concentrations of those showed a clear seasonal variation with the highest in winter and the lowest in summer. The correlations between the two were the best during the winter ($r^2=0.88$). As results of carbonaceous species analysis, the dominant factor in view of fine particle ($PM_{2.5}$) is primary emission source such as mobile, fossil fuel combustion during commute time(08:00~10:00 or 17:00~21:00) and winter season. Continuous monitoring of atmospheric carbonaceous species is essential to provide the science-based data to policy-maker establishing the air quality improvement policy.