• 한국어류학회지
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• 제25권4호
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• pp.216-221
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• 2013

2011년 12월부터 2012년 12월까지 수수미꾸리의 생식주기를 생식소중량지수와 조직학적 방법을 이용하여 조사하였다. 수수미꾸리 암컷과 수컷의 생식주기는 성숙기 (growing phase, 11~이듬해 3월), 산란기 (spawning phase, 4~5월), 퇴화기 (degeneration phase, 5~6월), 휴지기 (resting phase, 6~11월) 등 4단계로 구분되었다. 암컷과 수컷의 최적 수온이 $12.7^{\circ}C$, 일장시간이 13시간 이상으로 증가하기 시작하는 4월에서 5월 사이인 것으로 나타났다. 이러한 결과는 조직학적 실험 결과와 잘 일치하였다.

• 한국발생생물학회지:발생과생식
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• 제25권3호
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• pp.157-171
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• 2021

To determine whether the reproductive processes of sea bass, Lateolabrax japonicus, proceed normally after transportation from an outdoor net-cage into indoor tanks, we examined changes in the gonadosomatic index (GSI), histological gonadal tissue, and plasma levels of sex hormones (testosterone and estradiol-17ß) during their annual reproductive cycle. We also measured maturation and spawning across two sea water salinity levels (full and low salinity). Fecundity was estimated by the relationship between egg number and body size in female sea bass. Monthly changes in the GSI, histological gonadal tissues, and oocyte size showed both male and female sea bass reach final maturation in January and February, respectively, indicating that the spermiation of males occurs earlier than the spawning of females. The histological results indicated that the sea bass is a multiple spawner, similar to many marine teleosts, exhibiting group-synchronous oocyte development. Female maturation and spawning were enhanced in lower salinity seawater (29.6-31.0 psu) compared to that of normal salinity (34.5-35.1 psu). These results confirm that sea bass reproduction can occur successfully in captivity and imply that fertilized eggs can be collected from February to March. Additionally, our results show that lower salinity enhances oocyte maturation and spawning of female sea bass.

• Environmental Analysis Health and Toxicology
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• 제16권2호
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• pp.51-56
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• 2001

To evaluate the arsenic effect of fish endocrine disruption, crucian carp (Carassius auratus) were treated with 0.5 mg/L and 3.0 mg/L of As$_2$O$_3$ for 14 days and gonadal development was examined by histological analysis. In ovaries from female crucial carps exposed to 3.0 mg/L, immature follicles and atretic follicles were appeared while various normal developmental stages of oocytes were shown in control group. Body weight and Gonadosomatic Index (GSI) of treated carp were also decreased compared to control group. However, no other significant histological changes in liver or kidney were shown in this exposure scheme. This means that reproductive organs are more sensitive than other organs and arsenic may exert endocrine disrupting effect through inhibiting the development of reproductive organ in fish.

• Journal of Korean Neurosurgical Society
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• 제59권5호
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• pp.430-436
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• 2016

Objective : The objective of this study is to establish an animal model of chronic paraspinal muscle injury in rat. Methods : Fifty four Sprague-Dawley male rats were divided into experimental group (n=30), sham (n=15), and normal group (n=9). Incision was done from T7 to L2 and paraspinal muscles were detached from spine and tied at each level. The paraspinal muscles were exposed and untied at 2 weeks after surgery. Sham operation was done by paraspinal muscles dissection at the same levels and wound closure was done without tying. Kyphotic index and thoracolumbar Cobb's angle were measured at preoperative, 2, 4, 8, and 12 weeks after the first surgery for all groups. The rats were sacrificed at 4, 8, and 12 weeks after the first surgery, and performed histological examinations. Results : At 4 weeks after surgery, the kyphotic index decreased, but, Cobb's angle increased significantly in the experimental group (p<0.05), and then that were maintained until the end of the experiment. However, there were no significant differences of the kyphotic index and Cobb's angle between sham and normal groups. In histological examinations, necrosis and fibrosis were observed definitely and persisted until 12 weeks after surgery. There were also presences of regenerated muscle cells which nucleus is at the center of cytoplasm, centronucleated myofibers. Conclusion : Our chronic injury model of paraspinal muscles in rats shows necrosis and fibrosis in the muscles for 12 weeks after surgery, which might be useful to study the pathophysiology of the degenerative thoracolumbar kyphosis or degeneration of paraspinal muscles.

• 한국가축번식학회지
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• 제13권3호
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• pp.127-133
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• 1989

This study was undertaken to understand development stage of ovary and changes of hormones concentration from February to May in the Israeli carp, Cyprinus carpio. The results obtained in these experiments are as follows ; 1. Serum LH levels began to increase sharphy in March, coinciding with the onset of rapid ovarian development. 2. LH levels were well correlated with changes in gonadosomatic index. 3. Dramatic increase in gonadosomatic index occurred during the months of March. 4. Ripe stage(Stage Ⅴ) rapidly increase in March. 5. Early perinucleolus oocyte radpily develop into late perinucleolus oocytes in March. 6. The vitellogenic phase begins as these late perinucleolus oocytes become transformed into early maturing oocytes through the accumulation of yolk. 7. The cytoplasm completely fills with yolk as oocytes reach the late maturing stage. 8. Changes in the microscopic appearances of the ovaries were well correlated with changes in both gonadosomatic index and macroscopic appearance. 9. It is concluded from these observations that LH plays a major role in sexual maturation of the Israeli carp.

• Journal of Nutrition and Health
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• 제34권8호
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• pp.833-842
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• 2001

The purpose of this study is to determine the effect of dietary proteins and fats on the hepatic histological changes, membrane stability, and drug-metabolizing enzyme activities during chemically induced rat hepatocarcinogenesis. Weanling Sprague-Dawley rats were fed the diet containing 20% casein or soy protein isolate and 15% perilla or corn oil for 10 weeks. Hepatocarcinogensis was initiated with diethylnitrosamine(DEN), and the rats were fed diets containing 0.02% 2-acetylaminofluorene(AAF) followed by 0.05% phenobarbital (PB). The scores of histological changes were decreased in treated rats fed soy protein diet compared to those find casein diet. Liver weights were significantly increased by AAF and PB treatment in rats fed casein diets in both oil groups. Glucose 6-phosphatase(G6Pase) activities, an index of membrane stability, were significantly reduced by AAF and PB treatment in rats find casein diets, and were lower in casein diet compared to soy protein diet groups. Especially, the activities were the highest in the rats fed soy protein-perilla oil diet. Lipid peroxide values also were increased by AAF and PB treatment in rats fed casein diet. Aniline hydroxylase activities were not influenced by protein and fat sources. Glutathione-dependent enzyme activities were increased by AAF and PB treatment. Linoleic and arachidonic acid content were increased in rats fed corn oil diet, and linolenic and eicosapentaenoic acid contents were increased in rats fed perilla oil diet. Our results suggest that soy protein isolate inhibit the abnormal histological changes in liver, possibly by maintaining the membrane stability during chemically induced rat hepatocarcinogenesis. Soy protein may be protective against the hepatocarcinogenesis induced by chemical carcinogen.

• 한국패류학회지
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• 제23권2호
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• pp.199-208
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• 2007

2006년 1월부터 12월까지 전북 부안군 격포리에서 월별로 채집된 홍합, Mytilus coruscus를 대상으로 생식주기를 조사하였다. 홍합은 자웅이체이며, 난소는 수많은 난소소낭으로 이루어 져 있으며, 정소는 다수의 정소소낭으로 구성되어 있다. 생식소지수 (GI) 와 생식소중량지수 (GSI) 의 월별 변화는 2월에 최고값에 도달한 이후, 4월까지 점차 감소하였으며 GI 값과 GSI 값의 월별 변화는 서로 유사한 경향을 보였다. 비만도지수 (CI) 의 월별 변화는 생식소지수와 생식소중량지수의 변화양상과 유사한 경향을 나타내었다. 따라서 이들 세 가지 지수로부터 홍합의 산란기는 2-4월로 판단된다. 조직학적 조사에 의한 홍합의 산란기는 2-4월이었으며, 주 산란기는 2-3월이었다. 생식주기는 초기활성기 (11-1월), 후기활성기 (11-2월), 완숙기 (12-4월), 부분산란기 (2-4월), 퇴화기 (4-10월), 휴지기 (6-12월) 로 구분되었다. 각고에 따라 홍합의 군성숙도를 조사한 결과, 각고 40.1-50.0 mm인 개체들은 군성숙도 50% 이상 (암컷 71.4%, 수컷 78.9%) 이었고, 각고 50.1 mm 이상인 개체들은 군성숙도 100%이었다. 12개월간 채집한 총 개체들의 성비를 검증한 결과, 암수 성비는 1:1로 유의한 차이를 보이지 않았다 $(x^2\;=\;0.63,\;p\;>\;0.05)$.

• Journal of Korean Neurosurgical Society
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• 제30권4호
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• pp.430-436
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• 2001

Objective : The proliferative potential of intracranial glioma affects the histological malignancy and prognosis of patients with these tumors. In this study, we present the relationship between MIB-1 labeling index(LI) and clinical variables which might play the major role in determining the prognosis of patient with astrocytic tumors. Patients and Methods : Excised tumor specimens from a total of 52 patients were stained to detect monoclonal MIB-1-Ki-67 antibody by avidin-biotin complex immunohistochemistry. The MIB-1 LI was evaluated with histological grades, demograpghic data, and survival time. The statistical significance of their correlation was analyzed by Pearson correlation test. Results : The 52 patients included 30 male patients and 22 female patients. The tumors according to the criteria of the World Health Organization(WHO) classification were verified as pleomorphic xanthoastrocytoma in one, pilocytic astrocytomas 4, astrocytomas 1, anaplastic astrocytomas 3, and glioblastomas 31. MIB-1 LI in astrocytic ttumors showed no correlation with age and gender. However, the patients under 10 years had the longest survival time, whereas short survival time was observed in the older patients. The mean MIB-1 LI of different tumor grades were as follows : pleomorphic xanthoastrocytoma, $4.40{\pm}0.00$ ; pilocytic astrocytoma, $4.53{\pm}3.09$ ; astrocytoma, $5.50{\pm}6.03$ ; anaplastic astrocytoma, $12.68{\pm}12.50$ ; Glioblastoma, $21.31{\pm}19.63$. Although the levels of MIB-1 LI were varied in individual tumors, the MIB-1 LI was increased in parallel with the histological grades. Glioblstomas showed significantly higher MIB-1 LI compared with that of anaplastic astrocytomas and low grade astrocytomas (p = 0.001). The mean survival time of entire group of patients was also well correlated with MIB-1 LI in astrocytic tumors(p = 0.015). Moreover, the mean survival time of the entire group of patients with Lis < 10 was $125.33{\pm}113.57weeks$, and the mean survival of those with $Lis{\geq}10$ was $60.71{\pm}62.58weeks$. This difference was also statistically significant(p = 0.004). Conclusion : The results of this study suggest that MIB-1 LI correlates with histological grades and might play a significant role in predicting the survival of patients with astrocytic tumors.

• 보존과학회지
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• 제30권4호
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• pp.409-416
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• 2014

유적지에서 발굴되는 조선시대 인골은 보존 상태가 대부분 양호하여 형질인류학, 유전학, 그리고 화학적 연구를 수행할 수 있다. 본 연구에서는 김포 장기동 유적지에서 발굴된 조선시대 인골 6개체에 대한 DNA 분석을 통하여 미토콘드리아 DNA 변이형을 결정하였으며, 성별 분석결과를 토대로 남성 피장자의 Y 하플로그룹을 동정하였다. 유전자 분석에 앞서 보존상태가 양호한 것으로 판단되는 인골 6구를 대상으로 조직학 지수를 판별하였다. 미토콘드리아 DNA 변이형은 아시아인에서 높은 빈도로 나타나는 G, R11, M7, A5 등의 하플로그룹이 확인되었다. 인골의 성별을 확인하기 위하여 아밀로제닌 유전자 분석을 수행한 결과, 6구의 인골에서 여성 4구와 남성 2구의 성별을 확인할 수 있었다. 남성으로 판명된 인골에 Y 염색체 단일염기다형성 분석을 적용해본 결과, 하플로그룹 O로 확인되었다. 향후 출토 인골의 광범위한 분석을 위하여 인골을 선별한 경우에는 본 연구에서 제시한 조직학 지수를 적용하고, 미토콘드리아 DNA와 핵 DNA의 다양한 분석을 병행하여 출토 인골간의 유연관계를 규명하는 것이 필요하다.

• 환경생물
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• 제38권2호
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• pp.315-322
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• 2020

메기(Silurus asotus)의 기아 시 생존율, 생식소 중량지수(gonadosomatic index; GSI), 간 중량지수(hepatosomatic index; HSI) 및 장 중량지수(intestinosomatic index; ISI)와 신장세포, 장 상피세포 및 간세포의 조직학적 변화를 조사하였다. 생존율은 섭이군이 92.2±0.47%, 기아군이 74.4±2.59%를 보였으며, GSI, HSI 및 ISI는 기아군에서 가장 낮은 값을 나타내었다(p<0.05). 신장세포 핵의 높이, 장 상피세포 핵의 높이, 간세포 핵의 크기 및 간세포의 크기는 섭이군에서 가장 높은 값을 보였으며(p<0.05), 신장세포핵의 높이, 장 상피세포 핵의 높이 및 간세포 핵의 크기는 기아군에서 가장 낮은 값을 보였다(p>0.05). 멜라닌대식세포(melano-macrophages; MMs)는 기아 동안 신장에서 증가함을 관찰하였다. 신장의 MMs 증가 및 간세포의 수축현상은 메기의 기아 상태를 식별하는 지표로 이용할 수 있으며, 본 연구의 결과는 메기의 영양 상태 확인을 위한 기초 자료로서 의미가 있을 것으로 사료된다.