• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.32 no.5
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• pp.410-417
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• 2006

The present study was aimed to compare the resorption rate and the histological change of the autogenous dermis and the artificial dermis (Terudermis$^{(R)}$) after the transplantation, and to report the clinical results of the use of Terudermis$^{(R)}$ in order to restore the soft tissue defect. Twenty mature rabbits, weighing about 2 kg, were used for the experimental study. The autogenous dermis and the Terudermis$^{(R)}$ size 1${\times}$1 cm were transplanted to the space between the external abdominal oblique muscle and the external abdominal oblique fascia of the each rabbits. They were divided into 4 groups (n=5 each) and gathered at 1, 2, 4, and 8 weeks after the transplantation. The resorption rate was calculated, and H-E stain was preformed to observe the histological changes. The chart review of the 17 patients who received Terudermis$^{(R)}$ graft to the facial soft tissue defects was conducted for the clinical study. The resorption rate at 8 weeks after the transplantation was 21.5% for the autogenous dermis, and 36.4% Terudermis$^{(R)}$. In microscopic examinations, the infiltration of the inflammatory cells and the epidermal inclusion cyst were observed in the autogenous dermis graft. The neovascularization and the progressive growth of the new fibroblast were shown in the Terudermis$^{(R)}$ graft. In clinical data of 17 patients, the size of the grafted Terudermis$^{(R)}$ was from 1.5$cm^2$ to 7.5$cm^2$ (average 3.5$cm^2$). Follow-up ranged from 5 to 25 months. Fourteen patients with cleft palate demonstrated stability of the graft and unremarkable complications. But unstability of the graft and the partial relapse were observed in three patients received the vestibuloplasty. These results indicate that Terudermis$^{(R)}$ can be available substitute of autogenous dermis because of the stability about resorption, the histocompatibility, and the unremarkable clinical complications.

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.181-190
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• 2011

This study was investigated to find out the change of reproductive and histological biomarkers of Ruditapes philippinarum exposed to nonylphenol (NP). Experimental period was 16 weeks. Experimental groups were composed of one control condition and three NP exposure conditions (0.05, 0.1, $0.2 mg\;NP\;L^{-1}$). Gonad index was higher in exposure group than control in female and higher in control than exposure group in male. Intersexuality in the NP exposure group was 14.56% with male exhibiting higher ratio than female. Histological analysis of organ system illustrated expansion of hemolymph sinus, loss of striated border of inner epidermis and degeneration of mucous cell in the mantle. In the gill partially disappearance of frontal and lateral cilia, degeneration of mucous cell were observed. Also, hyperplasia of epithelium and disappearance of striated border were observed in the foot. As the concentration of NP increased, the accumulation of lipofuscin increased in the digestive gland. The results of this study suggest that NP induces reduction of survival rate, histopathology and intersex of R. philippinarum. Furthermore, it is indentified that NP functions as an estrogenic effector on the reproduction of R. philippinarum.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.225-232
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• 2013

This study looked into recovery rate and histological changes in the gills of juvenile abalone Haliotis discus hannai by exposure time (3, 6, 12, 24 and 48 h) of different water temperatures (15, 20 and $25^{\circ}C$) and salinities (30, 25, 20 and 15 psu) to understand reasons for the death of abalone exposed by low salinity water. In each water temperature, abalone spats that were exposed to low salinity water (less than 20 psu) for over 6 hours showed decrease in survival rate during recovery and those were exposed at the salinity of 15 psu for more than 24 hours all died. Histological observation showed expansion or damage of gills of the species which were exposed at less than 20 psu for over 6 hours. In case of abalones exposed at the salinity of 15 psu for over 24 hours, most gill tissues were destroyed. This result was glaringly obvious at a higher water temperature, lower salinity and longer exposure time. Accordingly, suffocation caused by damage of gills considered one of direct causes of the death.

• Toxicological Research
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• v.12 no.2
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• pp.213-221
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• 1996

In order to develop a suitable secondary renal disease model and diagnostic markers of renal disease in the rat, the change of PIIIP (aminoterminal procollagen III peptide) in serum and hydroxyproline levels in the renal tissue that reflect the synthesis of extracellular matrix (ECM) during development of experimental renal diseases were observed. Two types of experimental primary diseases, diabetes mellitus administrated by streptozotocin (STZ, 75 mg/kg, i.p.) and liver cirrhosis produced by bile duct ligation/scission (BDL/s) operation, were induced. The hydroxyproline level increased according to the high PIIIP and NCl(carboxyterminal procollagen IV peptide) in Western blot analysis as early as 1 week in the STZ treated-rat kidney. Increased renal ECM was observed at 15 weeks in STZ and BDL/s model under the microscopic examination. High PAS positive reaction was found in capillary basement membrane in STZ treated-rats and mesangium in BDL/s operated rats at this time, showing the histological characteristics of diabetic nephropathy and cirrhotic glomerulonephritis in human, respectively. Such secondary renal failure were supported by additional tests including urinalysis and renal function test. The serum PIIIP detected by ELISA was a useful parameter to estimate synthesis rate of renal ECM during development of renal disease without extrarenal fibrosis i.e. liver cirrhosis in rats. This study is proposed that STZ treatment or BDL/s operation may be a suitable experimental animal model for the induction and development of chronic secondary renal diseases. Morover, it was found that hydroxyproline level in renal tissues was a good parameter of the change of renal ECM at the early stage of the diseases without apparent histological changes. Especially, serum PIIIP could be a choice as a diagnostic or prognostic marker during the development of renal diseases in rats.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.1
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• pp.59-68
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• 2021

This study was performed to identify the effect of synovium graft on conjunctiva in rabbits after dry eye induction. Six New Zealand White rabbits were used as dry eye models. Both eyes were divided to two groups as control and synovium graft group. The synovium graft was performed in fourth week after dry eye modeling. Quantitative change of tears through Schirmer tear test (STT), qualitative change of tear film through tear film break up time (TFBUT), and damage of cornea through fluorescein staining were observed for 10 weeks at intervals of two weeks. Histological examination was performed to evaluate cornea and conjunctiva at tenth week. In both groups, STT and TFBUT were significantly decreased in 4 weeks after modeling compared to 0 weeks (p < 0.05) . After synovium graft, there were increases in STT value at 4 weeks and TFBUT at 4 and 6 weeks in graft group (p < 0.05). Corneal fluorescein staining showed no significant difference between the two groups. In histopathological examination, grafted synovium was detected as round to ovoid ingression folds, well attached to grafted regions with 0.11 ± 0.04 mm² (range, 0.05-0.16 mm²). These results indicated that the synovium graft on the conjunctiva had an effect on the qualitative and quantitative improvement of the tear film even though there was no histological change.

• Investigative Magnetic Resonance Imaging
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• v.20 no.4
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• pp.264-268
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• 2016

Benign fibrous histiocytoma (BFH) is a rare benign primary skeletal tumor that occurs commonly in the long bones, spine and pelvis. BFH constitutes a diagnostic challenge because it shares clinical background, radiological characteristics, and histological features with other fibrous lesions such as non-ossifying fibroma, giant cell tumor. We present a case of BFH with cystic change that occurred in the distal femur. We did not identify any case of BFH with cystic change involving the majority of the lesion that occurred in the metaepiphysis of the long bone.

• Korean Journal of Animal Reproduction
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• v.5 no.1
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• pp.43-63
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• 1981

This study aimed to determine the effects of adrenalectomy on the sexual gland, the thyroid gland and the serum components. A total of 192 Wister strain albino rats were evenly divided into 4 sexually equal groups for the comparisons. Each groups was divided into a control and a treatment group by sexuality. Each tissue of the sexual gland and the thyroid gland was microscopically examined, and at the same time body weight and serum components were also examined on the 1st, 7th, 14th, 28th, 42nd, 56th, 70th and 84th day respectively following adrenalectomy. The results obtained were as follows: 1. The body weight was sufficiently retarded in decreasingly after the adrenalectomy as compared to that of control. 2. The histological change of testis started atrophy of spermatogonia and degeneration of spermatocyte. There was a, pp.ared no cell division activity. Spermatozoa in the seminiferous tuble was not noticed but degeneration of interstitial cell was started and spermatozoa in the epididymal duct disa, pp.ared. 3. Degeneration of oocyte and follicular cell was noticed as the histological change of ovary. There was not a, pp.arance of primary follicle and corpus Iuteum but interstitial cell was proliferated. 4. For the effect of adrenalectomy on the histological change of the thyroid gland, the number of small follicle decreased and that of large follicle increased as the time passed following the adrenalectomy. And the follicular epithelial cell became squamous as typical condition of functional decreased. 5. It was shown that in the total contents of serum protein no difference with control occurred with in the 70th day for male and female adrenalectomized, respectively. But the differences in protein contents were significanly decreased on the 70th day. 6. No difference occurred in the total serum lipids on the 7th day, but they decreased significantly on the 42nd day, and (P<0.01) on the 56th, and 70th day. A tendency to decrease was noted as time elapsed following adrenalectomy. 7. Increase and decrease were intercrossed in the serum cholesterol contents between the control and th treatment groups on the 28th day. But the difference significantly decreased on the 42nd, 56th, and 70th day after adrelalectomized. The contents showed tendency to decrease as time elapsed following adrenalectomy. 8. The blood glucose contents rapidly decreased with time. The difference were significant on the 28th and 42nd day, and highly significant on the 56th and 70th day for male adrenalectomized. 9. In case of solium, there was no noticeable sexual distinction. There was slight in creasing or decreasing for the control group. But the treatment group tended continuously to decrease for male and female. 10. It was shown that potassium contents tended to increase on the 28th day for male and female, but the differences were small. 11. As for chlorine, it tended to decrease rapidly on 7th day for male and female adrenalectomized, and the tendency continued.

• Journal of Sasang Constitutional Medicine
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• v.9 no.2
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• pp.203-225
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• 1997

Jihwangbakhotang(地黃白虎楊) is made by Li Je Ma, the creator of the Four Constitutional Medicine. Single and 13 weeks oral repeated dose toxicity studies were conducted in Sprague Dawley rats of both sexes to elucidate the potential acute and subchronic toxicity of JBT extract and reversibility of any effects. In the single dose study, JBT extract was administered orally to rats with the dose of 2 g/kg and 8 g/kg. In the long term administration of 13 weeks, the JBT extract of 125 mg/kg/day, 500 mg/kg/day, 2000 mg/kg/day was administered to rats. The change of blood weight, urine volume, electrolyte in urine, hematological change, the change of blood chemistry, autopsy finding, and histological observation were researched, the results were as follows; 1. The lethal dose of JBT extract seems to be over 10 g/kg, the single administration of JBT extract 8 g/kg showed no toxical signs except little increase of urine volume. 2. The change of body weight had the trend of decrease in the group of, but has no significance, and also the consumption of food and water had no changes. 3. The hematological changes induced by the 13 weeks administration of JBT extract showed the significance in the item of Hb, MCH, MCV, WBC in the group of 125 mg/kg/day. 4. In the test of blood chemistry, total cholesterol showed little decrease and A/G ratio showed little increase, but the change was not clear, and the standard error was large. So the result was obtained insignificantly and the toxicity of JBT extract was not observed. 5. In the male group after recovery period, the level of cholesterol and triglyceride decreased slightly, but the result was not significant. 6. In the urine test, the little change of electrolyte was appeared, but it seemed not to be the result induced by the toxicity of JBT extract. 7. In each group of male and female rats, the weight change of organ and the serum histological changes was observed, but the result did not showed the dose dependent toxicity. So the toxicity of JBT extract was not regarded. In the conclusion, the toxicity of JBT extract was not observed in the single dose treatment and long term repetitive administration of JBT extract.

• Journal of Korean Neurosurgical Society
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• v.43 no.1
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• pp.34-36
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• 2008

Meningioma en plaque (MEP) is a rare tumor characterized more by its clinical and biological behavior than its histological appearance. Hyperostosis of the skull is one of the characteristic signs of MEP. This bony change can produce clinical symptoms and signs in MEP by pressing against adjacent structures. The authors report a rare case of an osteolytic MEP extending from the sphenoid wing into the orbital wall, middle fossa, and temporalis muscle.

• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2003.10a
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• pp.7-7
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• 2003

Histological examination of biopsy or postmortem lung tissue from patients with asthma usually reveals thickened airway walls. This change is called airway remodeling, which is characterized by airway eosinophilia, hyperplasia of goblet cells and smooth muscle, and subepithelial fibrosis [1,2]. In this study, we investigated the time-course functional, morphological, biochemical changes of remodeling in a ovalbumin (OVA)-induced murine chronic asthma model. (omitted)