• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.266-273
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• 2002

The dose dependence of the severity of radiation-induced thymic lymphoma in C57BL/6J mice was studied. Mice were exposed to fractionated irradiation at the total doses of 4.0, 6.0 and 8.0 Gy (four irradiations at 8-day intervals) starting from 33 days after birth. Pathological and histological changes of each mouse were observed after periodical sacrifice at day 75, 100, 125, 150, 175, 200, 250, 300 after the first irradiation. The severity of cancers were classified into 4 stages by clinical signs with respect to the enlargement of the thymus, spleen, liver, the progression of the cancer in the thymus, and the metastasis to the spleen, liver, lung and the lymphatic nodes. Among the 490 mice observed, 146 mice had thymic lymphoma. A clear dose-effect relationship was observed as well as the dose-response relationship. Also, periodical observation showed that thymic lymphoma was first induced in mice sacrificed at day 100 (130days old), and metastasize in the order of spleen, lung, liver and then the lymphatic nodes. The results suggest that radiation may be involved not only as a tumor initiator but also as a tumor promoter, and a tumor progression-enhancing agent.

• 한국패류학회지
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• 제29권3호
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• pp.225-232
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• 2013

본 연구는 저염분 해수에 노출된 전복의 폐사 원인을 구명하고자, 수온별 (15, 20 및 $25^{\circ}C$) 염분별 (30, 25, 20 및 15 psu) 노출시간별 (3, 6, 12, 24 및 48 h) 북방전복, Haliotis discus hannai 치패의 회복률 및 아가미의 조직학적 변화를 조사하였다. 모든 수온조건에서 20 psu 이하의 저염분 해수에 6시간 이상 노출시킨 전복은 회복기간 동안 생존율이 감소하였으며, 15 psu 해수에 24시간 이상 노출시킨 전복은 회복되지 못하고 전량 폐사하였다. 이를 조직학적으로 관찰한 결과, 20 psu 이하의 저염분 해수에 6시간 이상 노출시킨 전복의 아가미 조직은 팽창 또는 손상되었으며, 15 psu 해수에 24시간 이상 노출시킨 전복의 아가미 조직은 대부분 파괴되었다. 이와같은 결과는 수온이 높을수록, 염분이 낮을수록, 노출시간이 길수록 확연히 나타났다. 따라서 저염분 해수에 노출된 전복이 폐사하는 직접적인 원인 중의 하나는 아가미 조직의 손상으로 인한 질식으로 판단된다.

• Applied Biological Chemistry
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• 제25권4호
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• pp.201-205
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• 1982

감 과실(果實)의 탈삽기구(脫澁機構) 및 탄닌생성에 관한 조사의 일환으로 감엽(葉)의 탄닌함량의 변화 및 현미경적 관찰의 결과 삽시(澁枾)에서는 엽(葉)과 과실(果實)의 탄닌함량의 변화 경향이 유사하였으나, 감시(甘枾)에서는 많은 차이가 있었다. 엽(葉)의 탄닌함량 변화는 품종간에 큰 차이가 없었다. 엽(葉)의 상피세포(上皮細胞)는 과실(果實)의 탄닌세포와 같이 다량의 탄닌물질을 함유하고 있었으며 유관속(維管束) 부근에도 탄닌세포가 존재하였다. 사관부내(篩管部內)에는 탄닌물질이 이동하고 있었다. 이상의 결과 감엽(葉)의 탄닌물질과 과실(果實)의 탄닌물질은 밀접한 관계가 있음을 알 수 있었다.

• Archives of Pharmacal Research
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• 제10권1호
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• pp.42-49
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• 1987

This study is to evaluate the potential use of aclarubicin-loaded gelatin microspheres as an intravascular biodegradable drug delivery system for the regional cancer therapy. The diameter of the microspheres prepared by water in oil emulsion polymerization could be controlled by adjusting the stirring rate in the range of 10-50 $\mu$m : D(in $\mu$m) = -73.8 log (rpm) + 262.7. The addition of proteolytic enzyme increased the in vitro aclarubicin release but it did not change the amount of the initial burst release which reached about 45%. Microspheres injected intravenously into the mouse tail vein embolized only to the lung when observed by fluorescence microscopy. From histological examination following injection of gelatin microspheres into mouse femoral muscle, mild inflammation was observed from the appearance of neutrophils after 2 days and rapid repair process was confirmed thereafter. Biodegradation process of gelatin microspheres lodged on the pulmonary capillary bed was followed up by microscopic observation; degradation was taking place by about 36 hrs, followed by severe damage on the spheerical shape and microspheres was no longer found 10 days after injection.

• Natural Product Sciences
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• 제20권1호
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• pp.17-21
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• 2014

The combined new phytoformula (CAS), a mixture (5 : 3 : 2, w/w/w) of the ethanol extracts of the roots of C. officinalis and A. japonica, and the n-butanol fraction of the S. subprostrata strongly inhibited arthritic severity score as well as IL-6 production in serum of collagen-induced arthritic mice. Histological observation also indicated that the CAS-treated group showed less breakdown of joint cartilage of the collagen-treated mice. In contrast, C. officinalis alone or a combination of A. japonica and S. subprostrata did not show significant inhibitory action on the same animal model. Thus, it is thought that CAS possesses a synergistic inhibitory action on arthritic condition. All these results strongly suggest that CAS may be a potential anti-arthritic agent.

• 한국임상수의학회지
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• 제16권2호
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• pp.492-496
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• 1999

A 3-year old male Maltese, which had enlarged and ballooned superficial lymph-nodes, was admitted to the Veterinary Teaching Hospital, College of Veterinary Medicine, Kyung-pook National University. Radiographic findings indicated cranial mediastinal mass, interstitial pneumonitis, conspicuous splenomegaly and liver enlargement. The patient showed anorexia, depression and anemia. It was autopsied following its death 2 days after admittance. There were copious hemoperitoneum, lymphomatous mass in the spleen, torsion of the mesenterium and stenosis of the duodenum. On the microscopic observation, there was the increase of the amount of lymphocytic cytoplasm and numbers of nucleus-devided lymphocytes followed by their diffused infiltration in lymph nodule. Based on the physical, histological and pathological findings, this case was diagnosed as a multicentric form of lymphosarcoma.

• Environmental Analysis Health and Toxicology
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• 제17권1호
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• pp.7-11
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• 2002

Effects of nonylphenol (NP) on gonadal development and growth of olive flounder, Paralichthys olivaceus were investigated. NP treatment was carried out to fry fish (during 55 to 64 days after hatching) using oral adminstration at nominal NP concentrations of 50, 100 and 150 tig/g BW Gonad before NP treatment was sexually undifferentiated as observed with mostly gonia cells. At 159 days after hatching, ovarian lamella of ovary were filled with oogonia and perinucleolus oocytes. On the other hand, testicular lobules of the testis were occupied by spermatogonia, spermatocytes and spermatids. Histological observation of ovary and testis development was any different between the control and NP treated groups. However, growth was significantly increased in NP treated groups than control groups (P< 0.05). These results considerate that NP has any effect for sex differentiation and gonadal development, but act for early growth in olive flounder.

• 대한수의학회지
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• 제37권3호
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• pp.601-606
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• 1997

Mulberry heart disease was associated with vitamin E and selenium deficiency of pigs. Anatomical findings of this disease were hydropericadium, extensive patchy hemorrhage of epicardium, endocardium, and discoloration of dark red color of myocardium. In histological findings were characterized by acute myocardial degeneration, extensive hemorrhage, fibrinoid degeneration of arterioles, PAS positive material deposition of arterioles and capillary thrombosis. In affected herds, feed and serum tocopherol and selenium concentration were less than normal values.

• 대한미생물학회지
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• 제21권1호
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• pp.145-149
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• 1986

The development of delayed-type hypersensitivity(DTH) reaction to Staphylococcus aureus in mice was studied, Mice received 3 injections of $10^8$ viable S. aureus subcutaneously showed a marked footpad swelling when mice were challenged with $10\;{\mu}g$ staphylococcal protein antigen into footpad(The percent increase of footpad thickness at 24 h after challenge wsa 35% approximately). Histological observation of footpad of immunized mice showed a marked thickness of subcutaneous tissue due to edematous reaction and massive infiltration of lymphocytes and neutrophils which are characteristic cells in DTH reaction. Intensity of DTH reaction of mice immunized with viable bacteria was much higher than that of mice immunized with staphylococcal protein or heat-killed bacteria. The DTH reaction to S. aureus could be transferred to normal recipient mice by both spleen cells and lymph node cells.

• 대한수의학회지
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• 제23권2호
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• pp.183-186
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• 1983

The clinical and histopathological observation of leucocytozoonosis was carried out during July, 1982 at a poultry compound in Gyeongbuk province and the results were summarized as follows: 1. Leucocytozoonosis was observed in 36,900 of 59,900 chickens (61.6%) and the mortality rate was 18.3% (6,760 of 36,900 chickens infected). 2. Clinical findings were anorexia, dyspnea, paleness of comb, greenish diarrhea and reduction of egg production. 3. Gross lesions were yellowish white spots scattered on parenchymal organs and swelling of liver and spleen. In addition, petechiation and ecchymosis of small intestine, egg yolk and subcutaneous tissues were observed. 4. Histological findings were infiltration of mononuclear cells in liver, heart and lung, appearance of megaloschizonts in heart, liver, proventriculus and pancreas, proliferation of foreign body giant cells in heart, and hyperemia and swelling of all parenchymal organs.