• Journal of fish pathology
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• v.28 no.1
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• pp.9-15
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• 2015

The potential of Streptococcus iniae glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an antigen for a subunit vaccine was investigated using a zebrafish model. The recombinant S. iniae GAPDH was purified using His-tag column chromatography, and antisera against the recombinant GAPDH (rGAPDH) were produced by intraperitoneal immunization of rats. By immunization with S. iniae rGAPDH, the survival rates of zebrafish against an S. iniae challenge increased, suggesting that GAPDH would be an antigen capable of inducing protective immune responses in fish. Furthermore, we demonstrated using Western blotting, that the antisera against rGAPDH of S. iniae had cross-reactivity with GAPDH from Streptococcus parauberis and Lactococcus garviae, which are also culprits of streptococcosis in cultured fish in Korea. These results suggest that S. iniae GAPDH may be used as an antigen for the development of a subunit vaccine against streptococcosis caused by diverse cocci in cultured fish.

• English Language & Literature Teaching
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• v.9 no.1
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• pp.189-203
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• 2003

This paper attempts to prove Dickens's hopeful view of the future in his last completed novel Our Mutual Friend. This novel has been usually regarded as one of the "dark" novels, "dark" in the sense of viewing social reality and the future negatively. However, although it has the dark descriptive color of society typical in Dickens's later novels, it still contains some elements that point to a better future. To prove this positive view of future, this paper will disentangle the intricate narrative structure of Our Mutual Friend and find out the true meaning of the dust--money. In addition, it will investigate how people react to dust(-like money). From a close study of several characters' lives, it will testify that the dark world of Our Mutual Friend, in the end, could be a world of regeneration, a world that will lead to a better future.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.885-888
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• 2009

Radio Frequency IDentification (RFID) is a method of remotely storing and retrieving data using small and inexpensive devices called RFID tags. In this paper we propose a proxy agent framework that uses a personal device for privacy enforcement and increased protection against eavesdropping, impersonation and cloning attacks. Using the proxy model a user decides when and where information carried in a tag will be released. In particular, the user can put tags under her/his control, authenticated requests, release tags, transfer them to new owners, and so on. In this paper, we analyses a new type of simple a framework for enhancing RFID security by means of a proxy, a personal device that assumes control of a user's tags.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.639-642
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• 2003

Olfactory receptor protein ODR10 was expressed in E.coli as fusion protein with GST and His6 Tag. Crude membrane extract of the expressed protein was coated on the surface of quartz crystal microbalance, and the interaction of the ODR10 with several odorants was examined. Although the expression level was very low, quartz crystal microbalance showed that the expressed protein interacted most strongly with diacetyl (butanedione), which is known to bind to the ODR10 protein selectively. The interaction between ODR10 and diacetyl was $5{\sim}10$ times stronger than the interaction between ODR10 and other odorants. Thus, E. coli cells expressing the olfactory receptor protein could be used as an olfactory biosensor. Also, such system could be used to test which olfactory receptor reacts specifically with which odorant molecules, since there has been no cheap and convenient way to test the interaction of olfactory receptors and odorant molecules yet.

• Convergence Security Journal
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• v.12 no.4
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• pp.3-8
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• 2012

Smartphones have all the recent technology integration and NFC (Near Field Communication) Technology is one of them and become an essential these days. Despite using smartphones with NFC technology widely, not many security vulnerabilities have been discovered. This paper attempts to identify characteristics and various services in NFC technology, and to present a wide range of security vulnerabilities, prevention, and policies especially in NFC Contactless technology. We describe a security vulnerability and an possible threat based on human vulnerability and traditional malware distribution technic using Peer-to-Peer network on NFC-Enabled smartphones. The vulnerability is as follows: An attacker creates a NFC tag for distributing his or her malicious code to unspecified individuals and apply to hidden spot near by NFC reader in public transport like subway system. The tag will direct smartphone users to a certain website and automatically downloads malicious codes into their smartphones. The infected devices actually help to spread malicious code using P2P mode and finally as traditional DDoS attack, a certain target will be attacked by them at scheduled time.

• Journal of Microbiology and Biotechnology
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• v.24 no.11
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• pp.1503-1509
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• 2014

With the purpose of facilitating the process of stable strain generation, a shuttle vector for integration of genes via a double recombination event into two ectopic sites on the Sulfolobus acidocaldarius chromosome was constructed. The novel chromosomal integration and expression vector pINEX contains a pyrE gene from S. solfataricus P2 ($pyrE_{sso}$) as an auxotrophic selection marker, a multiple cloning site with histidine tag, the internal sequences of malE and malG for homologous recombination, and the entire region of pGEM-T vector, except for the multiple cloning region, for propagation in E. coli. For stable expression of the target gene, an ${\alpha}$-glucosidase-producing strain of S. acidocaldarius was generated employing this vector. The malA gene (saci_1160) encoding an ${\alpha}$-glucosidase from S. acidocaldarius fused with the glutamate dehydrogenase ($gdhA_{saci}$) promoter and leader sequence was ligated to pINEX to generate pINEX_malA. Using the "pop-in" and "pop-out" method, the malA gene was inserted into the genome of MR31 and correct insertion was verified by colony PCR and sequencing. This strain was grown in YT medium without uracil and purified by His-tag affinity chromatography. The ${\alpha}$-glucosidase activity was confirmed by the hydrolysis of $pNP{\alpha}G$. The pINEX vector should be applicable in delineating gene functions in this organism.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2011.10a
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• pp.811-814
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• 2011

This paper presents smart laundry management system using wireless Radio Frequnecy Identification (RFID) reader for efficient managing a large amount of laundry and clothing. The proposed system is comprised of wireless RFID hanger, base module connected on PC, and server system. The wireless RFID hanger with RFID reader read RFID tag attached on clothes and transmit tag information using wireless communication. The server system manage customers and his clothing using Database (DB) and display the information on the web page and smart phone devices. Since the proposed system operates with battery, we can evaluate its lifetime to measure current consumption. The designed system can be utilized to manage quantities of clothes and also it will be a good service to improve efficiency of laundry process.

• KIISE Transactions on Computing Practices
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• v.21 no.4
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• pp.283-289
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• 2015

We propose a low cost attendance checking system using NFC (Near Field Communication) and show a case study of an actual operation of the system in a higher education institute. The system offers a direct attendance check service when a student touches NFC tag on a classroom desk with his/her own smartphone. Our service was first developed and operated in 2012, and then additional functions like massive real time processing were reinforced. In the fall semester, 58 courses use the service and 96% of the class attendance was checked with mobile devices. The only hardware requirement of the system was NFC tag on the classroom desk, which reduced hardware cost dramatically. However, it also minimized attendance checking time into 1 minute regardless of enrolled student number.

• IMMUNE NETWORK
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• v.4 no.1
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• pp.23-30
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• 2004

Background: A human orthologue of mouse S100A6-binding protein (CacyBP), Siah-1-interacting protein (SIP) had been shown to be a component of novel ubiquitinylation pathway regulating $\beta$-catenin degradation. The role of the protein seems to be important in cell proliferation and cancer evolution but the expression pattern of SIP in actively dividing cancer tissues has not been known. For the elucidation of the role of SIP protein in carcinogenesis, it is essential to produce monoclonal antibodies specific to the protein. Methods: cDNA sequence coding for ORF region of human SIP gene was amplified and cloned into an expression vector to produce His-tag fusion protein. Recombinant SIP protein and monoclonal antibody to the protein were produced. The N-terminal specificity of anti-SIP monoclonal antibody was conformed by immunoblot analysis and enzyme linked immunosorbent assay (ELISA). To study the relation between SIP and colon carcinogenesis, the presence of SIP protein in colon carcinoma tissues was visualized by immunostaining using the monoclonal antibody produced in this study. Results: His-tag-SIP (NSIP) recombinant protein was produced and purified. A monoclonal antibody (Korea patent pending; #2003-45296) to the protein was produced and employed to analyze the expression pattern of SIP in colon carcinoma tissues. Conclusion: The data suggested that anti-SIP monoclonal antibody produced here was valuable for the diagnosis of colon carcinoma and elucidation of the mechanism of colon carcinogenesis.

• Applied Biological Chemistry
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• v.46 no.4
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• pp.353-360
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• 2003

For the simple rapid bioassay of organophosphorus and carbamate pesticide residues, a mass-production system of acetycholinesterase (AChE, EC 3.1.1.7, MAChE) using baculovirus and insect cell culture was constructed. The cDNA for AChE was synthesized from Drosophila melanogaster in Halla Mountain, the lipid anchor tail was removed by PCR and was used for the site-directed mutagenesis of three amino acid residues (E107Y, F368L, L408F). The mutated cDNA was inserted into the baculovirus vector and expressed in insect cells. Maximum cell growth and enzyme activity were reached when the cells $(2{\times}10^6\;cell/ml)$ were infected four times at four-day-intervals. His-tag containing MAChE was purified using Ni-NTA column and used for characterization. The activity was maintained under various pHs (3-10) and temperatures $(20-50^{\circ}C)$ under experimental conditions. As an extraction solution for pesticides, methanol is more effective than ethanol. Against major organophosphate and carbamate pesticides, the MAChE showed better sensitivity than AChE and AChE from housefly (Taiwan).