• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.313-314
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• 2009

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 추계학술발표회
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• pp.269.2-269.2
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• 2007

• 한국자원식물학회지
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• 제28권5호
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• pp.582-590
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• 2015

본 연구는 HRL의 3T3-L1 지방전구세포의 분화과정 중에 HRL이 지방의 축적에 미치는 영향을 확인하였다. MTT assay를 이용하여 세포 독성을 측정한 결과 100 ㎍/㎖의 농도에서도 세포증식에 영향을 미치지 않는 것을 확인하였고, 이와 같은 결과를 토대로 Oil Red O 염색법을 이용하여 지방세포 분화 억제능을 측정하였다. 그 결과, HRL의 경우 100 ㎍/㎖의 농도에서 82.25% 지방 축적 억제능을 나타내었다. 지방생성에 영향을 미치는 유전자 발현량을 측정하기 위해 RT-PCR법과 western blot법을 시행하였다. HRL은 SREBP-1c, PPARγ와 C/EBPα의 mRNA 발현을 억제시켰고, 지방생성에 영향을 미치는 효소인 FAS의 생성을 조절하는 것으로 나타났다. 또한, HRL 처리로 AMPKα의 단백질 발현이 증가하였으며, PPARγ의 발현량이 감소하는 것을 확인하였다. 이상의 결과들로부터 HRL은 AMPKα의 활성화를 통한 지방 합성을 억제를 보유하고 있는 바, 향후 항비만 기능성 소재로 활용될 수 있을 것으로 생각한다.

• 농업과학연구
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• 제49권4호
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• pp.893-904
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• 2022

It is well documented that the fruit of the vitamin tree, which is rich in vitamins A, C, E, and K, shows anti-diabetic, antibacterial, immune regulation, anti-inflammatory, and anti-aging effects. In some countries including Europe it has been used to develop various functional foods. This study was conducted to elucidate the fermentation properties of fermented milk supplemented with vitamin tree (Hippophae rhamnoides L.) fruit powder. The pH, titratable acidity, number of viable cells, and viscosity of the yogurt made with vitamin tree fruit powder were found to be higher than those of the control group, as the amount of supplemented powder added became higher and the fermentation progressed. Production of lactic, malic, and acetic acids increased relative to that of the control as the amounts of supplemented powder were higher. It was found that the increase in the decomposition of lactose to glucose and galactose was proportional to the amount of supplemented powder added and the elapsed fermentation time. During storage for 15 days the quality of yogurt supplemented with vitamin tree fruit powder was within the proper range of fermented milk quality in terms of pH, titratable acidity, and number of lactic acid bacteria. The yoghurt manufactured with vitamin tree fruit powder has a similar quality to that of the control, and therefore, a new functional yoghurt providing health benefits could be developed.

• 한국제4기학회지
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• 제17권2호
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• pp.123-128
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• 2003

This study was conducted to examine the water use efficiency (WUE) in five species of one-year-old seedlings grown in a field nursery in Mongolia. Larix sibirica and Pinus sylvestris are the most dominant coniferous species while Ulmus pumila is an important deciduous species known well-adapted in harsh conditions such as in semi-arid forests and Gobi desert regions. Caragana arborescens (Siberian pea shrub) and Hippophae rhamnoides are N-fixing shrubs in Mongolia. Thirty one-year-old seedlings were sampled from each of the five species (a total of 150 samples) and measured for net photosynthetic rate (Pn) and transpiration rate (E). The Pn and E were used to calculate and compare the WUE of each species. Pn differed significantly among the five species (p < 0.05). However, there was no significant difference in Pn between L. sibirica and H. rhamnoides (p > 0.05). C. arborescens showed the highest Pn whereas U. pumila did the poorest. E differed significantly among the five species (p < 0.05). L. sibirica and U. pumila showed considerably lower E than other species. Thus, WUE values of coniferous species such as L. sibirica and P. sylvestris were significantly greater than deciduous or shrub species such as U. pumila, C. arborescens and H. rhamnoides (p < 0.01). It may result that conifers showed relatively high water use efficiency than deciduous or shrub trees due to their lower transpiration rates, which resulted in morphological and physiological characteristics of their leaves. This may indicate that L. sibirica and P. sylvestris can be widely used for rehabilitation works in Mongolia attributed to their dominant distributions but also their high drought-resistance properties.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 추계학술발표회
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• pp.301.1-301.1
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• 2007

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 추계학술발표회
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• pp.283.1-283.1
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• 2007

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1257-1265
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• 2017

The present study was carried out to investigate the antioxidative and neuroprotective effects of sea buckthorn (Hippophae rhamnoides L.) leaves (SBL) harvested at different times. Reversed-phase high-performance liquid chromatography analysis revealed five major phenolic compounds: ellagic acid, gallic acid, isorhamnetin, kaempferol, and quercetin. SBL harvested in August had the highest total phenolic and flavonoid contents and antioxidant capacity. Treatment of neuronal PC-12 cells with the ethyl acetate fraction of SBL harvested in August increased their viability and membrane integrity and reduced intracellular oxidative stress in a dose-dependent manner. The relative populations of both early and late apoptotic PC-12 cells were decreased by treatment with the SBL ethyl acetate fraction, based on flow cytometry analysis using annexin V-FITC/PI staining. These findings suggest that SBL can serve as a good source of antioxidants and medicinal agents that attenuate oxidative stress.

• Journal of Forest and Environmental Science
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• 제37권2호
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• pp.148-153
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• 2021

This study was carried out to establish in vitro propagation system influenced by plant growth regulators through organogenesis with three different seed sources (China, Mongolia and Russia) for conservation of genetic resources in Northeast Asia. The experiment compared two different carbon sources (commercial sugar, sucrose), which showed no significant differences in germination rate. Induced adventitious buds from leaf segments were found to be highly effective when supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin, and 5.0 mg/L IAA, in the case of Chinese origin 96.8%, Russian origin R-1: 95.6%, R-2: 85.6%, and Mongolian origin M-2: 77.8%. It was effective in BA and Kinetin with supplemented with IAA, respectively. Shooting development was also efficient in Woody Plant Media (WPM) supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin and 5.0 mg/L IAA.

• 한국미생물·생명공학회지
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• 제40권1호
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• pp.43-49
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• 2012

비타민나무 잎 추출물 에틸아세테이트 분획물의 항균활성(MIC)을 측정한 결과 피부염의 원인인 S. aureus에서 0.06%로 methyl paraben(MP)의 0.25% 및 quercetin의 0.15%와비교하여 큰 항균활성을 나타내었으며, 여드름균인 P. acnes는 0.25%로 MP의 0.25% 및 quercetin 0.30%와 비슷한 항여드름균 활성을 나타내었다. 0.25% 비타민나무 잎 추출물을 함유한 크림의 12주 동안온도별 저장($4^{\circ}C$, $25^{\circ}C$, $37^{\circ}C$, $45^{\circ}C$) 및 태양광선 노출에서pH 결과는 0.08, 0.23 0.52, 1.34, 0.70 감소하여 안정적인 순서는 $4^{\circ}C$ > $25^{\circ}C$ > $37^{\circ}C$ > 태양광선 > $45^{\circ}C$로 측정되었다. 온도별 저장 조건에서 비교크림의 점도는 초기 점도보다 2,096 cP 낮은 았지만 저장 기간동안 점도의 변화가 대조크림의 변화보다 적은 감소폭을 나타내었으며 태양광선에 노출시킨 크림의 영향을 제외하고는 모두 대조크림과 비슷한 경향성을 나타내었다. 태양광선에서 대조크림의 점도가 감소하는 것과 비교해서 비교크림 점도의 증가는 비타민나무 잎 추출물이 태양광선에 대항하는 보호제로 크림의 점도를 유지시켜 제품을 안정화시키는데 기여한 것으로 사료된다. 비타민나무 잎 추출물의 에틸아세테이트 분획물을 에탄올에 용해시켜 4주 동안 저장 하였을 때 비타민나무 잎 추출물의 최대 흡수파장인 270 nm에서 관찰시 초기 흡광도에 비해 약 43% 감소하였고, 비교크림을 온도별로 저장하였을 때는 $4^{\circ}C$, $25^{\circ}C$에서 흡광도 변화는 각각 6%, 5%로 거의 변화가 없었다고 보여지며 $37^{\circ}C$, $45^{\circ}C$, 태양광선에 저장한 비교크림은 22%, 27%, 37% 감소하였다. 크리밍이나 응집, 합일과 같은 분리 현상이나 산화에 의한 특이취도 관찰되지 않았다. 이러한 결과들로부터, 비타민나무 잎의 에틸아세테이트 분획물은 0.25%에서 P. ovale, P. acnes, S. aureus에 대한 항균활성 나타내며 이에 따른 결과를 참고하여 비타민나무 잎추출물을 0.25% 함유한 크림은 비교적 안정함을 확인하였다. 향후 제품에 응용 시 사전 연구에서[8] 나타난 항산화및 미백과 주름개선에 대한 효과를 발휘할 수 있도록 보완연구가 이루어 진다면 화장품의 제품화가 가능할 것으로 사료된다.