• Journal of the Computational Structural Engineering Institute of Korea
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• 제32권6호
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• pp.409-416
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• 2019

An improved numerical model for non-linear analysis of reinforced concrete (RC) slabs subjected to blast loading is proposed. This approach considers a strain rate dependent orthotropic constitutive model that directly determines the stress state using the stress-strain relation acquired from the data obtained using the biaxial strength envelope. Moreover, the bond-slip between concrete and reinforcing steel is gradually enlarged after the occurrence of cracks and is concentrated in the plastic hinge region. The bond-slip model is introduced to consider the crack direction of the concrete under a biaxial stress state. Correlation studies between the numerical analysis and the experimental results were performed to evaluate the analytical model. The results show that the proposed model can effectively be used in dynamic analyses of reinforced concrete slab members subjected to explosive loading. Moreover, it was determined that it is important to consider biaxial behavior in the material model and the bond-slip effect.

• Molecules and Cells
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• 제44권1호
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• pp.1-12
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• 2021

The nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) is the master transcriptional regulator in adipogenesis. PPARγ forms a heterodimer with another nuclear receptor, retinoid X receptor (RXR), to form an active transcriptional complex, and their transcriptional activity is tightly regulated by the association with either coactivators or corepressors. In this study, we identified T-cell death-associated gene 51 (TDAG51) as a novel corepressor of PPARγ-mediated transcriptional regulation. We showed that TDAG51 expression is abundantly maintained in the early stage of adipogenic differentiation. Forced expression of TDAG51 inhibited adipocyte differentiation in 3T3-L1 cells. We found that TDAG51 physically interacts with PPARγ in a ligand-independent manner. In deletion mutant analyses, large portions of the TDAG51 domains, including the pleckstrin homology-like, glutamine repeat and proline-glutamine repeat domains but not the proline-histidine repeat domain, are involved in the interaction with the region between residues 140 and 506, including the DNA binding domain, hinge, ligand binding domain and activation function-2 domain, in PPARγ. The heterodimer formation of PPARγ-RXRα was competitively inhibited in a ligand-independent manner by TDAG51 binding to PPARγ. Thus, our data suggest that TDAG51, which could determine adipogenic cell fate, acts as a novel negative regulator of PPARγ by blocking RXRα recruitment to the PPARγ-RXRα heterodimer complex in adipogenesis.

• Journal of the Computational Structural Engineering Institute of Korea
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• 제34권6호
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• pp.393-401
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• 2021

In this paper, we proposed a numerical model based on moment-curvature, to evaluate the resistance of reinforced concrete (RC) members subjected to blast loading. To consider the direct shear failure mode, we introduced a dimensionless spring element based on the empirical direct shear stress-slip relation. Based on the dynamic increase factor equations for materials, new dynamic increase factor equations were constructed in terms of the curvature rate for the section which could be directly applied to the moment-curvature relation. Additionally, equivalent bending stiffness was introduced in the plastic hinge region to consider the effect of bond-slip. To verify the validity of the proposed model, a comparative study was conducted against the experimental results, and the superiority of this numerical model was confirmed through comparison with the analytical results of the single-degree of freedom model. Pressure-impulse (P-I) diagrams were produced to evaluate the resistance of members against bending failure and direct shear failure, and additional parametric studies were conducted.

• Journal of the Computational Structural Engineering Institute of Korea
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• 제35권5호
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• pp.287-297
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• 2022

This paper presents experimental and analytical studies on the lateral cyclic behavior of RC columns actively confined with iron-based shape memory alloy (Fe-SMA) strips. Based on the Anexperimental study, we investigated the effectiveness of active confinement through compression testings of concrete cylinders confined by Fe SMA strips and carbon fiber-reinforced polymer (CFRP) sheets. The test results showed that the specimens confined with Fe SMA strips significantly increased the deformation capacity of the concrete, even under lower confining pressures, compared to those specimensconfined with CFRP sheets. The experimental results were used to develop finite-element models of RC columns confined with Fe SMA or CFRP in their plastic-hinge region. After validating the proposed analytical model through comparison with the results from a previous RC column test, a series of lateral cyclic load analyses were carried out for the RC columns confined with Fe SMA and CFRP. The analytical results revealed that the lateral cyclic behavior of the Fe SMA-confined column was greatly enhanced in terms of deformation and energy dissipation capacities compared with tothat of the as-built and CFRP-confined columns.

• Earthquakes and Structures
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• 제26권1호
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• pp.31-48
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• 2024

In order to get a better understanding of seismic performance of exterior beam-column joint, reciprocating loading tests with variable loading speeds or axial forces were carried out. The main findings indicate that only few cracks exist on the surface of the joint core area, while the plastic hinge region at the beam end is seriously damaged. The damage of the specimen is more serious with the increase of the upper limit of variable axial force. The deflection ductility coefficient of specimen decreases to various degrees after the upper limit of variable axial force increases. In addition, the higher the loading speed is, the lower the deflection ductility coefficient of the specimen is. The stiffness of the specimen decreases as the upper limit of variable axial force or the loading speed increase. Compared to the influence of variable axial force, the influence of the loading speed on the stiffness degradation of the specimen is more obvious. The cumulative energy dissipation and the equivalent viscous damping coefficient of specimen decrease with the increase of loading speed. The influence of variable axial force on the energy dissipation of specimen varies under different loading speeds. Based on the truss model, the biaxial stress criterion, the Rankine criterion, the Kent-Scott-Park model, the equivalent theorem of shearing stress, the softened strut-and-tie model, the controlled slip theory and the proposed equations, a calculation method for the shear capacity is proposed with satisfactory prediction results.

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10255-10262
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• 2015

High risk human papillomavirus (HR-HPV) E2 proteins play roles in transcriptional regulation and are commonly functionally disrupted when the HPV genome integrates into host chromosomes. Some 15-40% of cancer cases, however, contain an intact E2 gene or episomal HPV. In these cases, polymorphism of the E2 gene might be involved. This study aimed to determine polymorphisms of the E2 gene in episomal HPV16 detected in high grade squamous intraepithelial lesions and squamous cell carcinomas and altered functions compared to the E2 prototype. The E2 gene was amplified and sequenced. Two expression vectors containing E2 gene polymorphisms were constructed and transfected in SiHa and C33A cells, then E6 gene as well as Il-10 and TNF-${\alpha}$ expression was determined by quantitative RT-PCR. Expression vectors and reporter vectors containing the HPV16 long control region (LCR) were co-transfected and transcriptional activity was determined. The results showed that a total of 32 nucleotides and 23 amino acids were changed in all 20 cases of study, found in the transactivation (TA) domain, hinge (H) region and DNA binding (DB) domain with 14, 5 and 13 nucleotide positions. They mostly caused amino acid change. The expressing vectors containing different E2 gene polymorphisms showed E6 mRNA suppression, TNF-${\alpha}$ mRNA suppression and IL-10 induction but no statistically significant differences when compared to the E2 prototype. Moreover, promoter activity in HPV16 LCR was not affected by E2 protein with different gene polymorphisms, in contrast to nucleotide variations in LCR that showed an effect on transcription activity. These results demonstrated that E2 gene polymorphisms of episomal HPV16 did not affect transcriptional regulation and suggested that nucleotide variation as well as epigenetic modification of the LCR might play a role in inducing malignant transformation of cells containing episomal HPV16.

• Earthquakes and Structures
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• 제8권3호
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• pp.665-679
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• 2015

The strain rate of reinforced concrete (RC) structures stimulated by earthquake action has been generally recognized as in the range from $10^{-4}/s$ to $10^{-1}/s$. Because both concrete and steel reinforcement are rate-sensitive materials, the RC beam-column joints are bound to behave differently under different strain rates. This paper describes an investigation of seismic behavior of RC beam-column joints which are subjected to large cyclic displacements on the beam ends with three loading velocities, i.e., 0.4 mm/s, 4 mm/s and 40 mm/s respectively. The levels of strain rate on the joint core region are correspondingly estimated to be $10^{-5}/s$, $10^{-4}/s$, and $10^{-2}/s$. It is aimed to better understand the effect of strain rates on seismic behavior of beam-column joints, such as the carrying capacity and failure modes as well as the energy dissipation. From the experiments, it is observed that with the increase of loading velocity or strain rate, damage in the joint core region decreases but damage in the plastic hinge regions of adjacent beams increases. The energy absorbed in the hysteresis loops under higher loading velocity is larger than that under quasi-static loading. It is also found that the yielding load of the joint is almost independent of the loading velocity, and there is a marginal increase of the ultimate carrying capacity when the loading velocity is increased for the ranges studied in this work. However, under higher loading velocity the residual carrying capacity after peak load drops more rapidly. Additionally, the axial compression ratio has little effect on the shear carrying capacity of the beam-column joints, but with the increase of loading velocity, the crack width of concrete in the joint zone becomes narrower. The shear carrying capacity of the joint at higher loading velocity is higher than that calculated with the quasi-static method proposed by the design code. When the dynamic strengths of materials, i.e., concrete and reinforcement, are directly substituted into the design model of current code, it tends to be insufficiently safe.

• Journal of the Korea Concrete Institute
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• 제24권6호
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• pp.705-714
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• 2012

Precast reinforced concrete bridge columns with hollow rectangular section were tested under cyclic lateral load with constant axial force to investigate its seismic performance. After all the precast column segments were erected, longitudinal reinforcement was inserted in the sheath prefabricated in the segments, which were then mortar grouted. Main variables of the test series were column aspect ratio, longitudinal reinforcement ratio, amount of lateral reinforcement, and location of segment joints. The aspect ratios were 4.5 and 2.5, and the longitudinal steel ratios were 1.15% and 3.07%. The amount of lateral reinforcement were 95%, 55%, 50%, and 27% of the minimum amount for full ductility design requirements in the Korean Bridge Design Code. The locations of segment joints in plastic hinge region were 0.5 and 1.0 times of the section depth from the bottom column end. The test results of cracking and failure mode, axial-flexural strength, lateral load-displacement relationship, and displacement ductility are presented. Then, safety of the ductility demand based seismic design in the Korean Bridge Design Code is discussed. The column specimens showed larger ductility than expected, because buckling of longitudinal reinforcing bar was prevented due to confinement developed not only by transverse steel but also by sheath and infilling mortar.

• Journal of Korean Society of Steel Construction
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• 제19권6호
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• pp.587-597
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• 2007

Steel restrainer cables for multiple frame bridges in California in the United States have been shown to be effective in preventing unseating at internal hinges during the past several earthquakes. Consequently, the steel-cable-restrainer is being tested for applications on multiple-span-simply-supported (MSSS) bridges in the mid-American region. In addition, shape memory alloy (SMA) bars in tension are being studied for the same application, multiple frame bridges, the developed seismic forces are transferred to piers through the restrainers. However, in MSSS bridges, the seismic forces are transferred to abutments by the restrainers. Therefore, the abutment' behavior should also be investigated. In this study, we assessed the seismic performance of the three types of restrainers, such as steel restrainer cables, SMA in tension, and SMA in bending for an MSSS bridge from moderate to strong ground motion, bending test of an SMA bar was conducted and its analytical model was determined for this study. Nonlinear time history analyses were conducted to assess the seismic responses of the as-built and the retrofitted bridges. All three types of restrainers reduced the hinge opening and the SMA in tension was the most effective of the three devices in preventing the unseating, all restrainers produced damage on the abutment from the pulling action of the MSSS bridge due to strong ground motions, was found that the retrofit of the abutment in the pulling action is required in the installation of restrainers in MSSS bridges.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제14권2호
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• pp.927-935
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• 2013

Recently, bridge structures has progressed the researches about seismic performance by occurrence of earthquake increased compared with the past. In the substructure of bridge, confining transverse reinforcement has arranged in plastic hinge region to resist the lateral load which increased the lateral confining effect. Columns are increased the seismic performance through secure of the stiffness and ductility The design specification for arrangement of confining transverse reinforcement same specification of domestic and international that suggested to solid reinforced concrete column(RC). This design specification have limits for Internally Confined Hollow RC(ICH RC) column because of different the component and performance characteristics of column. In this paper suggested the modified equation for economics and rational design through investigation of displacement ductility when applied the existing specification at the steel composite hollow RC column.