• Title/Summary/Keyword: Higher fungi

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Quality Changes in Apple Juice Containing Pulp upon Sterilization by Hot Water (열수 살균 조건에 따른 과육 함유 사과주스의 품질 변화)

  • Park, Nan-Young;Kim, Jae-Whoa;Woo, Sang-Cheul;Jeong, Yong-Jin
    • Food Science and Preservation
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    • v.17 no.2
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    • pp.230-235
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    • 2010
  • We investigated the physicochemical quality characteristics of apple juice upon sterilization using hot water, and under various storage conditions. None of sugar content, acidity, or pH differed significantly among various sterilization conditions but chromaticity was considerably reduced in sterilized juice compared with control material. The chromaticity of non-sterilized juice decreased significantly after sterilization compared with material supplemented with vitamin C (0.1%, w/v). Fungi, yeast, and aerobic bacteria were detected in juice sterilized at $65^{\circ}C$ for 10 or 20 min, but no microorganisms were observed in juice sterilized by other procedures. Vitamin C content affected sterilization temperature to a greater extent than sterilization time. When juice was stored at $4^{\circ}C$ or $37^{\circ}C$ for 3 weeks after application of different sterilization conditions, almost no change in acidity, sugar content, or pH was observed, regardless of sterilization mode or storage period. However, chromaticity decreased with longer storage. Vitamin C levels were reduced by higher sterilization temperatures. However, longer storage periods had the greatest effect on reduction of vitamin C levels, which tended toward lower values regardless of differences in sterilization and storage conditions. In sensory evaluation tests, all of taste, color, and overall preference were highest for juice sterilized at $75^{\circ}C$ for 20 min. The ASC value was low at a storage temperature of $4^{\circ}C$ and at high sterilization temperatures, and a long storage period was associated with a greater ASC value. Thus, the quality of apple juice was excellent when juice was hot water-sterilized, with additional vitamin C, at $75^{\circ}C$ for 20 min, followed by storage at $4^{\circ}C$.

A Correlation Between Fibrinolytic Activity and Microflora in Korean Fermented Soybean Products (된장의 혈전용해효소 활성과 미생물 분포와의 상관관계)

  • 김동호;송현파;김기연;김정옥;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.1
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    • pp.41-46
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    • 2004
  • A correlation between fibrinolytic activity and microflora in Korean traditional soybean fermented food was investigated. The fibrinolytic activities of traditional soybean pastes and commercially processed samples were 2.42$\pm$1.01 unit/g and 1.58$\pm$0.98 unit/g, respectively. The cell density of Bacillus in traditional soybean pastes was about 10$^{7}$ CFU/g and its commercially processed one was 10$^{6}$ CFU/g. Acid producing bacteria, fungi and yeast group were higher in commercially processed one. The correlations of fibrinolytic activity and microflora in traditional and commercial Doenjang were positively correlated in Bacillus ($R^2$≒ 0.69), negatively correlated in fungal group ($R^2$≒0.40), and there were no significant correlations in acid forming bacteria and yeast group ($R^2$<0.16). Fibrinolytic activities in Meju and Koji were 6.54$\pm$1.97 unit/g and 1.46$\pm$0.43 unit/g respectively, and were positively correlated with Bacillus. Yeast and acid forming bacteria were grown by 5∼6 decimal induction during fermentation period of Doenjang, but Bacillus, fungal cells and fibrinolytic activity were nearly stable. Results indicate that fibrinolytic activity of Doenjang depends on enzyme induction in Meju or Koji processing by Bacillus, Doenjang fermentation process.

Development of Drainage Water Disinfection System by Electric Shock in Recirculating Soilless Culture (순환식 수경재배에서 배액의 전기충격살균법 연구)

  • Lee, Mun Haeng;Kim, Sung Eun;Lee, Sang Don;Lee, Jae Eun;Kim, Hak Sun;Cho, Suk Keong;Sim, Sang Youn;Kim, Young Shik
    • Journal of Bio-Environment Control
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    • v.25 no.1
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    • pp.49-56
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    • 2016
  • This study was conducted to develop the disinfection system using electric shock for recycled nutrient solution in recycling soilless culture. Stainless steel (SUS 316) was found as the most appropriate electrode material for electrical disinfection system from the view of high electrical conductivity, low electric resistance, and low price. There were no changes in nutritional elements when the electric shock passed through the nutrient solution by stainless steel electrode. The amount of electric current increased with width than thickness of the electrode. The farther the distance between the electrodes was increased the time to reach out the aimed amount of current. The electric shock was applied to Ralstonia solanacearum and Fusarium oxysporum as representative bacteria and also fungi. Any of those pathogens were killed with the percentage of higher than 97% in the condition of 15VDC or 24VDC.

The Transformation of Saponin Platycodi Radix by Aspergillus niger and Anti-oxidation Evaluation of the Transformed Metabolites (Aspergillus niger 효소에 의한 길경 사포닌(플라티코딘)의 전환 및 항산화 활성 비교)

  • Kang, Ju-Hui;Ji, Gnu-Uk;Wui, Hye-Jung;Hwang, In-Kyeung
    • Korean journal of food and cookery science
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    • v.24 no.6
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    • pp.729-734
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    • 2008
  • The principal objective of this study was to assess the possibility of transforming platycodin glycosides using various strains of probiotic bateria and edible fungi. Among the experimental microorganisms assess herein, Aspergillus niger KCTC 6909 evidenced the highest level of platycodin glycoside hydrolysis during fermentation. Particularly in cases in which the organism was incubated in the presence of rhamnose and platycodins. In order to produce the enzyme from Aspergillus niger effectively, various incubation conditions were assessd in order to determine the optimal conditions. The cytotoxicity on V79-4 (Chinese- hamster lung fibroblasts, normal cells) of platycodin was reduced significantly after conversion (concentration on $500{\mu}g/mL$, $1000{\mu}g/mL$); DPPH radical scavenging activity before conversion was 35.05%, and was 57.44% afterward. We noted significantly higher conversion activity inhibiting oxidative degradation. In conclusion, these results indicate that the proper combination of food microorganisms -and fermentation conditions can result in an increase in the glycoside hydrolysis of platycodin the resultant products of which reduce cytotoxicity- and increase anti-oxidant activity.

Effects of Water-logging on the Chemical Properties, Microflora and Biomass in Continuous Cropping of Cucumber Soils (오이연작토양(連作土壤)의 화학성(化學性), 미생물상(微生物相) 및 Biomass 에 미치는 담수처리(湛水處理)의 영향(影響))

  • Kim, Hyun-Woo;Kim, Young-Woong;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.22 no.2
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    • pp.146-155
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    • 1989
  • A series of the experiments were conducted to evaluate the effects of the water-logging in continuous cropping soils of cucumber. Some of the results obtained from these experiments are summarized as follow: The values of pH in water-logged treatments were from 5.50 to 5.90. Those of EC were less than 0.3 mmhos/cm, were lower than those of control plot. The ratios of Ca/Mg, Ca/K, Mg/K respectively 2.5, 4.8, 2.0, were similar to the standard values. These changes were suitable for cucumber planting. And the number of bacteria among the microflora were determined increased from $10^6$ to $10^7\;cells/g$ dry soil, while that of the Fungi, Fusarium spp, Pythium spp, and Phythophthora spp decreased. In proportion to these changes of microflora, the ratios of B/F in water-logging plot were higher than in the control plot. The contents of Biomass-C were showed 10.47mg/100g soil to 36.12 mg/100g soil, and the contents of that in water-logging were more than in control plots.

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Effects of Compost and Rice Straw Application on Growth of Soybean Plant in Newly Reclaimed Upland Soil (신개간지(新開墾地) 토양(土壤)에서 퇴비(堆肥)와 볏짚시용(施用)이 대두생육(大豆生育)에 미치는 영향(影響))

  • Lee, Myong-Gu;Hwang, Kwang-Nam
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.3
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    • pp.199-206
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    • 1982
  • A field experiment was conducted to compare the effects of compost and rice straw application on the growth of soybean, chemical properties and soil microorganism in newly reclaimed silty clay loamy upland soil. Application rates of the arganic materials were 750 Kg, 1,500 Kg and 3,000 Kg/10a in compost, and 340 Kg, 680 Kg and 1,360 Kg/10a in rice straw. The differences of N.P.K. application rates caused by the two different organic materials were balanced by chemical fertilizers of N.P.K. The results can be summarized as follows; 1. The yield of soybean was increased upon compost application, but no significant difference on yield was observed except luxuriant plant growth upon rice straw application. 2. There was highly possitive correlation between concentration of phosphorous, potassium in soybean plant at 71 days after planting and dry weight of grain vs. stem plus bean chaff of harvest plant in compost applied plots, but no correlation was found in rice straw applied plots. 3. In greneral, the soil water contents in organic material applied plots, expecially in plots of rice straw, were higher than in no organic material plots. However, at wilting point, the soil water content in organic material applied plot was lower compare to no organic material plot. 4. The weight of nodules per soybean plant was heavier notwithstanding the fewer number of soil bacteria and fungi in compost application plots than rice straw application plots.

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Effect of Plant-growth-promoting Bacteria Inoculation on the Growth and Yield of Cucumber(Cucumis sativa L.) (식물생육촉진 세균이 오이 생육 및 수량에 미치는 영향)

  • Lee, Young-Han;Cho, Woo-Suk;Kim, Jong-Gyun;Lee, Han-Saeng;Park, Sang-Ryeol;Yun, Han-Dae
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.196-199
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    • 1997
  • We studied the effect of inoculation of microorganisms known to produce plant growth promoting substances, on the growth and yield of cucumber(Cucumis sativa L.), through a field experiment. The microorganisms used were isolated from the forest soil and consisted of Micrococus sp., Baccilus sustilis, Enterobacter agglomerans, Baccilus megaterium, Pseudomonas putida, Cellulomonas sp. and Staphylococus xyposus. Fotr the multiplication, microorganisms were cultured in liquid media of Pseudomonas P and Sabouraud dextrose. Inoculation of microorganisms was done by spraying the culture media after the culture of them to soil and cucumber plants, three times during the growth of cucumber at the rate of 10l/ha. The inoculation of microorganisms tended to promote the growth of cucumber plant and increase the yield of it. No sign of significant improvement of soil chemical and physical properties were observed after the harvest of crop. The population of bacteria and actinomycetes tended to be higher in the inoculated plots than in not inoculated plots, while opposite was the case in the population of fungi.

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Enzyme Inhibition Activities of Ethanol Extracts from Germinating Rough Rice (Oryza sativar L.) (발아기간에 따른 벼(Oryza sativa L.)의 부위별 효소저해활성)

  • Kim, Min Young;Lee, Sang Hoon;Jang, Gwi Young;Park, Hye Jin;Meishan, Li;Kim, Shinje;Lee, Youn Ri;Lee, Junsoo;Jeong, Heon Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.6
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    • pp.917-923
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    • 2013
  • This study investigated the enzyme inhibitory effects of ethanol extracts from the different parts of rough rice (Oryza sativar L.) from its germination period. Rough rice was germinated at $37^{\circ}C$ for 6 days, then separated into hull+sprout and brown rice. ${\alpha}$-Glucosidase inhibitory activity had the highest value (39.38%) in hull+sprout extracts after 5 days of germination. ${\alpha}$-Amylase and DPP-4 inhibitory activity had the highest values (75.32% and 47.77%, respectively) in hull+sprout extracts after germination for 5 days. ACE inhibitory activity of hull+ sprout extracts increased from 43.16% at the beginning to 58.60% at 5 days, while brown rice extracts increased this activity from 0.88% at the beginning to 14.50% at 4 days. The xanthine oxidase inhibitory activity of hull+ sprout extracts increased from 62.02% at the beginning to 64.49% at one day, and then decreased. Lipase inhibitory activity had its highest value (55.17%) in hull+sprout extracts after germination for 5 days. These results indicate that the optimal germination period for increasing enzyme inhibitory activities may be 5 days, and that hull+sprout extracts have a higher enzyme inhibition activity than brown rice.

Use of an Artificial Neural Network to Construct a Model of Predicting Deep Fungal Infection in Lung Cancer Patients

  • Chen, Jian;Chen, Jie;Ding, Hong-Yan;Pan, Qin-Shi;Hong, Wan-Dong;Xu, Gang;Yu, Fang-You;Wang, Yu-Min
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.12
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    • pp.5095-5099
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    • 2015
  • Background: The statistical methods to analyze and predict the related dangerous factors of deep fungal infection in lung cancer patients were several, such as logic regression analysis, meta-analysis, multivariate Cox proportional hazards model analysis, retrospective analysis, and so on, but the results are inconsistent. Materials and Methods: A total of 696 patients with lung cancer were enrolled. The factors were compared employing Student's t-test or the Mann-Whitney test or the Chi-square test and variables that were significantly related to the presence of deep fungal infection selected as candidates for input into the final artificial neural network analysis (ANN) model. The receiver operating characteristic (ROC) and area under curve (AUC) were used to evaluate the performance of the artificial neural network (ANN) model and logistic regression (LR) model. Results: The prevalence of deep fungal infection from lung cancer in this entire study population was 32.04%(223/696), deep fungal infections occur in sputum specimens 44.05%(200/454). The ratio of candida albicans was 86.99% (194/223) in the total fungi. It was demonstrated that older (${\geq}65$ years), use of antibiotics, low serum albumin concentrations (${\leq}37.18g/L$), radiotherapy, surgery, low hemoglobin hyperlipidemia (${\leq}93.67g/L$), long time of hospitalization (${\geq}14$days) were apt to deep fungal infection and the ANN model consisted of the seven factors. The AUC of ANN model($0.829{\pm}0.019$)was higher than that of LR model ($0.756{\pm}0.021$). Conclusions: The artificial neural network model with variables consisting of age, use of antibiotics, serum albumin concentrations, received radiotherapy, received surgery, hemoglobin, time of hospitalization should be useful for predicting the deep fungal infection in lung cancer.

Medium characteristics during the outdoor-composting stage of medium preparation with a prototype medium turner in button mushroom cultivation (양송이 배지교반기 시제품을 활용한 배지 조제시 야외 발효단계별 배지의 특성)

  • Lee, Chan-Jung;Yu, Byeong-Kee;Lee, Eun-Ji;Park, Hae-Sung;Kong, Won-Sik;Kim, Yeong-Ho
    • Journal of Mushroom
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    • v.16 no.1
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    • pp.45-50
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    • 2018
  • This study was performed to compare medium characteristics during the composting stage for medium turning performed using an excavator agitator and a prototype medium turner in button mushroom cultivation. The changes in temperature in the medium did not significantly differ between the treatments until the 3rd turn performed using the excavator agitator. However, during the 4th and 5th turns, the temperature increased during turning with the prototype medium turner. During outdoor composting, various types of microorganisms such as thermophilic bacteria (Bacillus spp.), Actinomycetes, fluorescent Pseudomonas spp., and filamentous fungi were found to be distributed in the medium. The counts of aerobic bacteria and fluorescent Pseudomonas spp. did not significantly differ between treatments, and the counts of thermophilic bacteria and thermophilic actinomycetes were slightly higher during turning with the prototype medium turner. The rice straw was slightly shorter and water content lower for the prototype medium turner. There was no significant difference between pH and EC treatments. The L, a, and b values tended to increase on turning with the prototype medium turner.