• Asian Pacific Journal of Cancer Prevention
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• v.15 no.18
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• pp.7547-7552
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• 2014

The mir-155 family is not only involved in a diversity of cancers, but also as a regulator of the immune system. However, the evolutionary history of this family is still unclear. The present study indicates that mir-155 evolved independently with lineage-specific gain of miRNAs. In addition, arm switching has occurred in the mir-155 family, and alternative splicing could produce two different lengths of ancestral sequences, implying the alternative splicing can also drive evolution for intragenic miRNAs. Here we screened validated target genes and immunity-related proteins, followed by analyzation of the mir-155 family function by high-throughput methods like the gene ontology (GO) and Kyoto Eneyclopedin of Genes and Genemes (KEGG) pathway enrichment analysis. The high-throughput analysis showed that the CCND1 and EGFR genes were outstanding in being significantly enriched, and the target genes cebpb and VCAM1 and the protein SMAD2 were also vital in mir-155-related immune reponse activities. Therefore, we conclude that the mir-155 family is highly conserved in evolution, and CCND1 and EGFR genes might be potential targets of mir-155 with regard to progress of cancers, while the cebpb and VCAM1 genes and the protein SMAD2 might be key factors in the mir-155 regulated immune activities.

• Journal of odor and indoor environment
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• v.16 no.1
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• pp.54-63
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• 2017

The Automobile HVAC system is a habitat for odor-associated fungal communities. We investigated the odor-associated fungal community in an automobile HVAC system using a high-throughput DNA sequencing method. The fungal community structure was evaluated via metagenome analysis. At the phylum level, Ascomycota and Basidiomycota were detected, accounting for 43.41% and 56.49% of the fungal community in the HVAC system, respectively. Columnosphaeria (8.31%), Didymella (5.60%), Davidiella (5.50%), Microxyphium (4.24%), unclassified Pleosporales (2.90%), and Cladosporium (2.79%) were abundant at phylum of Ascomycota and Christiansenia (36.72%), Rhodotorula (10.48%), and Sporidiobolus (2.34%) were abundant at phylum of Basidiomycota. A total of 22 genera of fungi were isolated and identified from the evaporators of the HVAC systems which support fungal growth and biofilm formation. Among them, Cladosporium, Penicillium, Aspergillus, and Alternaria are the most representative odor-associated fungi in HVAC systems. They were reported to form biofilm on the surface of HVAC systems with other bacteria by hypha. In addition, they produce various mVOCs such as 3-methyl-1-butanol, acetic acid, butanoic acid, and methyl isobutyl ketone. Our findings may be useful for extending the understanding of odor-associated fungal communities in automobile HVAC systems.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.8 no.2
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• pp.95-101
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• 2022

Microfluidic radioimmunoassay (RIA) platform called µ-RIA spends less reagent and shorter reaction time for the analysis compared to the conventional tube-based radioimmunoassay. This study reported the design of µ-RIA chips optimized for the gamma counter which could measure the small samples of radioactive materials automatically. Compared with the previous study, the µ-RIA chips developed in this study were designed to be compatible with conventional RIA test tubes. And, the automatic gamma counter could detect radioactivity from the ¹²⁵I labeled anti-PSA attached to the chips. Effects of the multi-layer microchannels and two-phase flow in the µ-RIA chips were investigated in this study. The measured radioactivity from the ¹²⁵I labeled anti-PSA was linearly proportional to the number of stacked chips, representing that the radioactivity in µ-RIA platform could be amplified by designing the chips with multi-layers. In addition, we designed µ-RIA chip to generate liquid-gas plug flow inside the microfluidic channel. The plug flow can promote binding of the biomolecules onto the microfluidic channel surface with recirculation in the liquid phase. The ratio of liquid slug and air slug length was 1 : 1 when the ¹²⁵I labeled anti-PSA and the air were injected at 1 and 35 µL/min, respectively, exhibiting 1.6 times higher biomolecule attachment compared to the microfluidic chip without the air injection. This experimental result indicated that the biomolecular reaction was improved by generating liquid-gas slugs inside the microfluidic channel. In this study, we presented a novel µ-RIA chips that is compatible with the conventional gamma counter with automated sampler. Therefore, high-throughput radioimmunoassay can be carried out by the automatic measurement of radioactivity with reduced radiowaste generation. We expect the µ-RIA platform can successfully replace conventional tube-based radioimmunoassay in the future.

• Bulletin of the Korean Chemical Society
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• v.25 no.12
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• pp.1833-1839
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• 2004

Recently mass spectrometry and separation methods such as liquid chromatography have become major tools in the field of proteomics. In this report, we describe in detail our efforts to develop ultra-high pressure capillary reverse-phase liquid chromatography (cRPLC) and its online coupling to a mass spectrometer by a nanoelectrospray (nanoESI) interface. The RPLC system is constructed in house to deliver LC solvents at the pressure up to 20,000 psig, which is four times higher than conventional RPLC systems. The high operation pressure allows the efficient use of packed micro-capillary columns (50, 75 and 150 ${\mu}$m i.d., up to 1.5 m long). We will discuss the effect of column diameter on the sensitivity of cRPLC/MS/MS experiments and the utility of the developed technique for proteome analysis by its application in the analysis of proteome samples having different levels of complexity.

• Journal of Environmental Health Sciences
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• v.40 no.1
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• pp.55-62
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• 2014

Objectives: $^{32}P$-postlabeling assay is the most sensitive method of detecting DNA adduct formation. However, it is limited by a low sample throughput and use of radioisotopes (RI). In this study, we modified it to minimize these limitations and applied it to Z. platypus exposed to Benzo(a)pyrene (BaP) in order to investigate DNA adduct formation (effect biomarker for pollutants) in Z. platypus for assessing risk of waterborne BaP exposure. Methods: DNA hydrolysis was performed only with Micrococcal nuclease (MNase), RI reduction test was performed and the overlapping steps between thin layer chromatography (TLC) and radioisotope high-performance liquid chromatography (RI-HPLC) were omitted. The application of a modified method to Z. platypus exposed to BaP was performed. Results: The results revealed that the amount of RIs used can be reduced roughly 10-fold. Because the analysis time was shortened by 8.5 hours, the sample throughput per hour was increased compared with the previous method. The results of applying modified $^{32}P$-postlabeling assay to Z. platypus, DNA adduct formation in Z. platypus showed dose-dependency with the BaP concentration. Only BPDE-dGMP was detected as a DNA adduct. Conclusion: These results demonstrate that the modified $^{32}P$-postlabeling assay is a suitable method for detecting DNA adduct formation in Z. platypus exposed to waterborne BaP and will be useful in risk assessment of carcinogenic effect in aquatic environment due to BaP.

• Journal of Internet Computing and Services
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• v.1 no.2
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• pp.1-10
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• 2000

ATM network technology is generally used for the solution of integrating multimedia service in high-speed Internet. In Internet protocol based on ATM services, If single cell is lost in ATM layer, the entire TCP packet will be lost. Therefore, TCP performance will be degraded. In order to reduce cell loss, when congestion occur, UBR+EPD mechanism is proposed to improve the throughput in TCP over UBR, and ER scheme is suggested in TCP over ABR. In this paper, we analyzed the performance improvement effect of UBR+EPD with FRR (Fast Retransmission and Recovery), the adjusting EPD threshold parameter (R), and variation of MTU (Maximum Transport Unit) size. As a result, through the analysis of performance, we know that the improved throughput and fairness are shown by the proposed scheme.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.44 no.3 s.357
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• pp.41-50
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• 2007

In this paper, a new Dual Round-Robin (DRR) based iterative SLIP (iSLIP) scheduling scheme, called DiSLIP is proposed for IP switching systems. By using DRR followed by iSLIP, DiSLIP can exploit desynchronization effect of DRR and high performance of iSLIP, while the drawbacks of two schemes are minimized. 'Through computer simulation, we verify the switch throughput and total waiting time of the proposed scheme under nonuniform and correlated self-similar traffic. Moreover, the proposed scheme can considerably reduce the complexity of parallel matching logics compared to iSLIP. From the result, we observe that the proposed scheme outperforms DRR on throughput as well as iSLIP schemes on complextiy.

• The KIPS Transactions:PartC
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• v.11C no.6 s.95
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• pp.787-798
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• 2004

High performance net재rking is one of key factors to provide support for data intensive applications in the Internet. Extended frame size has a major impact on end to-end performance with increasing effective TCP throughput and decreasing system overhead. Most of the research about extended frames has focused on local area network performance and the impact that extended frame size has on the system elements including memory, network interface card and so forth. In the paper, we analyse the effects of the extended frames to the other traffic flows sharing Internet paths for the wide area performance of TCP by conducting various network simulations. Results show that securing available bandwidth in no loss and low delay networks is indispensable to exploit the efficiency of extended frames.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.4 no.4
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• pp.509-528
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• 2010

The random access systems, with retransmission diversity (RD) employment, over large random spreading code division multiple access (CDMA) channel subject to fading is investigated, under the assumption of infinite number of users and infinite spreading gain with their ratio converging to a constant. The low bound of the signal to interference and noise ratio (SINR) is shown to converge almost surely to a constant. The throughput, spectrum efficiency and energy efficiency in the dominating systems are obtained. The analytical results are confirmed by simulations. We find that in high traffic loads the throughput with fading is higher than that without. When the energy efficiency increases, the spectrum efficiency tends to two contrary values due to SNR increases or decreases. For the ordinary stable systems, the stability region is shown to shrink as the traffic increases and enlarge with RD employment.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.9
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• pp.4242-4263
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• 2017

Multi-User Multiple-Input Multiple-Output (MU-MIMO) technology has recently attracted significant attention from academia and industry because of it is increasingly important role in improving networks' capacity and data rate. Moreover, MU-MIMO systems for the Fifth Generation (5G) have already been researched. High Quality of Service (QoS) and efficient operations at the Medium Access Control (MAC) layer have become key requirements. In this paper, we propose a downlink MU-MIMO MAC protocol based on adaptive Channel State Information (CSI) feedback (called MMM-A) for Wireless Local Area Networks (WLANs). A modified CSMA/CA mechanism using new frame formats is adopted in the proposed protocol. Specifically, the CSI is exchanged between stations (STAs) in an adaptive way, and a packet selection strategy which can guarantee a fairer QoS for scenarios with differentiated traffic is also included in the MMM-A protocol. We then derive the expressions of the throughput and access delay, and analyze the performance of the protocol. It is easy to find that the MMM-A protocol outperforms the commonly used protocols in terms of the saturated throughput and access delay through simulation and analysis results.