• 제목/요약/키워드: High-through Sequence

검색결과 423건 처리시간 0.035초

원통형 문풀 구조물의 탑재 시 변형과 수정 (Welding Deformation and Its Correction of Cylindrical Moon Pool Structure)

  • 성우제;천광산
    • 대한조선학회논문집
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    • 제56권5호
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    • pp.389-395
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    • 2019
  • Turret mooring type Floating Production Storage and Offloading (FPSO) is designed to rotate the hull around a turret system. The system is mounted inside a cylindrical moon-pool structure of the ship hull structure. The upper part of the moon-pool structure called Bogie Support Structure (BSS) is supported on ring type rail structure (bogie), so high roundness is required at the top of the structure. In this study, the deformation measured during BSS installation was compared with the predicted values through the thermal elasto-plastic analysis, and the causes of deformation were analyzed. Deformation behavior of cylindrical structure with a very large diameter compared to the thickness was investigated. In addition, a proper welding sequence and correction method for the deformed structure were proposed. This study can be an example of the solution to the tolerance problem of large cylindrical structures.

Identification and Phylogeny of the Human Endogenous Retrovirus HERV-W LTR Family in Cancer Cells

  • Yi, Joo-Mi;Kim, Hwan-Mook;Kim, Heui-Soo
    • Animal cells and systems
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    • 제6권2호
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    • pp.167-170
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    • 2002
  • The long terminal repeats (LTRs) of human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of closely located genes. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. We examined the HERV-W LTR elements in various cancer cells (2F7, A43l , A549, HepG2, MIA-PaCa-2, PC-3, RT4, SiHa, U-937, and UO-31). Using genomic DNA from the cancer cells, we performed PCR amplification and identified twelve new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (88-99%) with HERV-W LTR (AF072500). A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-W LTR elements could be mainly divided into two groups through evolutionary divergence. Three HERV-W LTR elements (RT4-2, A43l-1, and UO3l-2) belonged to Group 1, whereas nine LTR elements (2F7-2, A549-1, A549-3, HepG2-3, MP2-2, PC3-1, SiHa-8, SiHa-10, and U937-1) belonged to Group 11. Taken together, our new sequence data of the HERV-W LTR elements may contribute to an understanding of tissue-specific cancer by genomic instability of LTR integration.

SOX 유전자의 다양성 (Multiple Facets of Sox Gene)

  • 홍경원;김희수
    • 생명과학회지
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    • 제14권4호
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    • pp.716-725
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    • 2004
  • Sox 패밀리는 동물계 전체에서 찾아지는 전사인자이고, HMG라는 특이적인 DNA결합 도메인을 가진다. 이 Sox 패밀리는 HMG 도메인의 아미노산 서열을 바탕으로 현재 10개의 그룹으로 분류된다. 각 그룹의 오소로그한 Sox 단백질들은 선충에서 인간까지 상당한 보존성을 보인다. HMG 도메인은 전사 촉진 좌위에 결합하고 다른 전사인자들의 결합을 조절함으로써 동물 발생과정의 다양한 세포에서 발현되어 그들의 분화에 결정적인 영향을 미친다. 최근 많은 분자 생물학자들이 Sox 유전자와 관련된 질병, 진화, 그리고 계통 분류 등에 많은 관심을 보이고 있다. 특히, 줄기세포에서 Sox 유전자의 연구는 그들의 생물학적인 기능을 이해하기 위해 꼭 필요한 분야이다. 아마도 이 Sox 유전자들을 이해함으로써, 인간의 유전적 질병과 인간을 포함한 전체 동물계의 진화를 이해할 수 있는 열쇠가 될 것이라 생각한다.

Genome Scale Protein Secondary Structure Prediction Using a Data Distribution on a Grid Computing

  • Cho, Min-Kyu;Lee, Soojin;Jung, Jin-Won;Kim, Jai-Hoon;Lee, Weontae
    • 한국생물물리학회:학술대회논문집
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    • 한국생물물리학회 2003년도 정기총회 및 학술발표회
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    • pp.65-65
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    • 2003
  • After many genome projects, algorithms and software to process explosively growing biological information have been developed. To process huge amount of biological information, high performance computing equipments are essential. If we use the remote resources such as computing power, storages etc., through a Grid to share the resources in the Internet environment, we will be able to obtain great efficiency to process data at a low cost. Here we present the performance improvement of the protein secondary structure prediction (PSIPred) by using the Grid platform, distributing protein sequence data on the Grid where each computer node analyzes its own part of protein sequence data to speed up the structure prediction. On the Grid, genome scale secondary structure prediction for Mycoplasma genitalium, Escherichia coli, Helicobacter pylori, Saccharomyces cerevisiae and Caenorhabditis slogans were performed and analyzed by a statistical way to show the protein structural deviation and comparison between the genomes. Experimental results show that the Grid is a viable platform to speed up the protein structure prediction and from the predicted structures.

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New Control Strategy for Three-Phase Grid-Connected LCL Inverters without a Phase-Locked Loop

  • Zhou, Lin;Yang, Ming;Liu, Qiang;Guo, Ke
    • Journal of Power Electronics
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    • 제13권3호
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    • pp.487-496
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    • 2013
  • The three-phase synchronous reference frame phase-locked loop (SRF-PLL) is widely used for synchronization applications in power systems. In this paper, a new control strategy for three-phase grid-connected LCL inverters without a PLL is presented. According to the new strategy, a current reference can be generated by using the instantaneous power control scheme and the proposed positive-sequence voltage detector. Through theoretical analysis, it is indicated that a high-quality grid current can be produced by introducing the new control strategy. In addition, a kind of independent control for reactive power can be achieved under unbalanced and distorted grid conditions. Finally, the excellent performance of the proposed control strategy is validated by means of simulation and experimental results.

LFSR과 CAT을 이용한 영상 암호화 (Image Encryption using LFSR and CAT)

  • 남태희;김석태;조성진
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2009년도 춘계학술대회
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    • pp.164-167
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    • 2009
  • 본 논문에서는 LFSR(Linear Feedback Shift Register)과 2D CAT(Two-Dimensional Cellular Automata Transform)를 이용한 영상 암호화법을 제안한다. 먼저 LFSR을 이용하여 원 영상의 크기만큼 PN(pseudo noise) 수열을 생성한다. 그런 다음, 생성된 수열을 원 영상과 XOR 연산하여 원 영상을 변환한다. 그리고, 게이트웨이 값을 설정하여 2D CAT 기저함수를 생성한다. 생성된 기저함수를 변환된 원 영상에 곱하여 2D CAT 영상 암호화를 한다. 마지막으로, 안정성 분석을 통하여 제안한 방법이 높은 암호화 수준의 성질을 가졌음을 검증한다.

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Metal-Poor F-G-K type Local Subdwarfs From SDSS + GAIA GR2: Spectrophotometric & Kinematic Properties

  • Yang, Soung-Chul;Kim, Young Kwang;Lee, Young Sun;Lee, Hogyu
    • 천문학회보
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    • 제43권2호
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    • pp.59.2-59.2
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    • 2018
  • We introduce a new project of constructing a large spectro-photometric samples of metal-poor (i.e. [Fe/H] < -1.0) subdwarfs in the Galactic halo. The sample is collected from a compilation of the stellar objects that are cross-identified both in the Sloan Digital Sky Survey (SDSS) and recently published data from GAIA mission. The color range of the selected stars covers 0.0 < (g-r) < 2.0; thus the spectral types of our sample span from early F- through late K-type stars on the metal-poor main sequence (i.e. the local subdwarf sequence). We scrutinized the physical, chemical, and kinematical properties of our samples using their SDSS medium-resolution (R ~ 2000) spectra, combined with accurately measured proper motions from GAIA satellite. Our study will provide useful information on the global trend in the various properties (e.g. abundance pattern as a function of the galactocentric distance; rotational velocity vs [Fe/H] ${\cdots}$ etc) of the metal-poor subdwarf populations in the Galactic halo, which is ultimately important to better understand metal-poor stellar evolutionary models and chemical evolution of the Milky Way halo in the early phase of its formation. Further our comprehensive catalog of the Galactic field halo subdwarfs collected in this study will serve a solid groundwork for future follow-up high resolution spectroscopic observations on many interesting individual targets.

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Identification of three independent fern gametophytes and Hymenophyllum wrightii f. serratum from Korea based on molecular data

  • LEE, Chang Shook;LEE, Kanghyup;HWANG, Youngsim
    • 식물분류학회지
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    • 제50권4호
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    • pp.403-412
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    • 2020
  • Colonies of three independent gametophytes (one that is filamentous and two that are ribbon-like) without sporophytes occur in Gyeonggi-do, Gangwon-do, Gyeongsang-do, and Jeju-do, Korea. They have a moss-like appearance at first sight, with tiny plantlets and gemmae, and grow in cool, shaded, relatively deep dint places of large rocks, such as the small caves in high mountains, close to valleys. The gametophytes were identified based on morphological and molecular data by chloroplast DNA (cpDNA) sequence data (rbcL, rps4 gene and rps4-trnS intergenic spacer). Here, rbcL, rps4 gene and rps4-trnS intergenic spacer data of one independent gametophyte distributed in Korea have the same morphology, DNA sequence and monophyletic group as Crepidomanes intricatum from the eastern United States. They also share the same cpDNA data with Crepidomanes schmidtianum recently reported from Korea. The other independent gametophyte should be Hymenophyllum wrightii based on cpDNA data. The last one was presumed to be Pleurosoriopsis makinoi based on molecular data. The taxonomic status was confirmed to be the forma of Hymenophyllum wrightii through a revision of Hymenophyllum wrightii f. serratum based on molecular data.

Long Short Term Memory 모델 기반 Case Study를 통한 낙동강 하구역의 용존산소농도 예측 (Prediction of DO Concentration in Nakdong River Estuary through Case Study Based on Long Short Term Memory Model)

  • 박성식;김경회
    • 한국해안·해양공학회논문집
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    • 제33권6호
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    • pp.238-245
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    • 2021
  • 본 연구에서는 LSTM 모델을 활용하여 낙동강 하구역의 DO 농도 예측을 위한 최적 모델 조건과 적합한 예측변수를 찾기 위한 Case study를 수행하였다. 모델 매개변수 case study 결과, Epoch = 300과 Sequence length = 1에서 상대적으로 높은 정확도를 보였다. 예측변수 case study 결과, DO와 수온을 예측변수로 했을 때 가장 높은 정확도를 보였으며, 이는 DO 농도와 수온의 높은 상관성에 기인한 것으로 판단된다. 상기 결과로부터 낙동강 하구역의 DO 농도 예측에 적합한 LSTM 모델 조건과 예측변수를 찾을 수 있었다.

Pseudomonas sp. MN5의 특성과 망간산화단백질 정제 (Characterization of Pseudomonas sp. MN5 and Purification of Manganese Oxidizing Protein)

  • 이승희;박경량
    • 생명과학회지
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    • 제18권1호
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    • pp.84-90
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    • 2008
  • 충청남도 병천면 일대의 6곳의 토양시료를 채취하여 망간을 산화하는 균주들을 순수분리 하고, 이 중 망간 산화능이 가장 우수한 한 균주를 최종 선별하여 본 실험에 사용하였다. 최종 선별된 균주의 생리, 생화학적 특성을 조사하고, 16S rRNA 염기 서열분석 등을 통하여 동정한 결과 최종 선별된 균주는 Pseudomonas sp. MN5로 확인되었다. Pseudomonas sp. MN5은 fructose와 maltose를 제외한 다양한 당을 이용하지 못하였으며, 항생제인 kanamycin, chloramphenicol, streptomycin 그리고 tetracycline에는 높은 감수성을 보이고, 리튬, 망간, 바륨과 같은 중금속에 대해서는 mg/ml 단위의 높은 내성을 나타냈다. 그리고 Pseudomonas sp. MN5의 망간산화 최적 pH는 7.5이고, 망간산화 활성이 proteinase K와 가열처리를 한 시료에서 저해되었다. Pseudomonas sp. MN5가 생성하는 망간산화 단백질을 ammonium sulfate precipitation, HiTrap Q FF ion exchange chromatography 그리고 G3000sw $_{XL}$ gel filtration chromatography를 통해서 정제한 결과, 15 kDa, 46.7 kDa 그리고 63.5 kDa의 세종류의 manganese oxidizing protein가 확인되었고, 내부서 열과 N-말단 서열 분석 결과 Pseudomonas sp. MN5가 생성하는 망간산화 단백질은 외막의 porin 단백질인 것으로 추정되었다.