• Journal of Preventive Medicine and Public Health
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• 제27권1호
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• pp.84-106
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• 1994

Hyperbaric oxygen (HBO) therapy for carbon monoxide (CO) poisoning eventually inducing the hypoxia-reoxygenation condition, may produce oxygen free radicals, which forms 8-hydroxydeoxyguanosine (8-OH-dG) by attacking C-8 position of deoxyguanosine (dG) in DNA. Effects of oxygen partial pressure or duration of HBO therapy with or without CO poisoning on the tissue 8-OH-dG formation were investigated. Male Sprague-Dawley rats were grouped and exposed to air (control group), 4000 ppm of CO for 10 to 30 minutes (CO only group), air for 30 minutes after 30 minute exposure to 4000 ppm of CO(CO-air exposure group), HBO after 30 minute exposure to 4000 ppm of CO(CO-HBO group), or HBO therapy fo. $10{\sim}120$ minutes(HBO only group). The 8-OH-4G concentrations in the brain and the lung tissues were measured with high performance liquid chromatography and electrochemical detector (ECD). Average concentrations of the 8-OH-dG of each group were statistically compared. In the brain tissues, 8-OH-dG concentrations of the CO only group, the CO-air exposure group, and the CO-HBO group did not significantly differ from those of the control group. Similar insignificance was also found between the CO-HBO group and the HBO only groups. No appreciable dose-response relationship was observed between the 8-OH-dG concentration and the oxygen partial pressure or the duration of HBO. However, the 8-OH-dG concentrations of the 30 minute CO only group were higher than those of the CO-air exposure group (p-value<0.05). In the lung tissues, there were no significant differences between the 8-OH-dG concentrations of the control group and those of the CO only group, the CO-air exposure group, and the CO-HBO group. However, mean 8-OH-dG concentration of the CO-air exposure group was significantly higher than that of the CO only group under the same CO exposure condition(p-value<0.05). With the duration of CO exposure, the 8-OH-dG concentrations of the lung tissues decreased significantly (p-value<0.05). The concentrations of 8-OH-dG in the lung tissues proportionally increased with the duration of HBO, but no such relation was observed with the oxygen partial pressure. These results suggest that the brain may be more resistant to oxygen free radicals as compared with the lungs, and that oxygen toxicity following HBO may be affected by factors other than oxygen free radicals.

• 대한조선학회논문집
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• 제39권3호
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• pp.57-63
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• 2002

심해자원채광시스템은 모선, 양광관, 버퍼, 집광기 등으로 구성된다. 양광관의 위쪽은 모선과 연결되고 아래쪽은 버퍼에 연결된다. 버퍼와 집광기는 유연 호스로 연결된다. 채광시스템의 원활한 운영을 위해서는 전체 시스템의 안정화가 이루어져야 하는데 이를 위해서는 버퍼의 위치를 능동적으로 제어해야한다. 본 연구에서 가장 중요한 부분은 길이 5000m에 달하는 양광관의 동적 거동해석이다. 양광관을 세장구조물로 단순화시켜 모델링하고 고유함수 전개법으로 해석하였으며, 버퍼에 장착되는 추진시스템에 대한 제어에는 PID 제어기법을 적용하였다. 버퍼에 작용하는 외력은 양광관에 작용하는 항력에 의한 반력이 지배적이므로 본 논문에서는 유연호스의 영향은 무시하였다. 파랑 중에서 모선은 저주파수 운동과 동시에 고주파 운동을 하게 되는데 버퍼가 추적하고자 하는 모선의 위치는 저주파수 운동에 해당하므로 고주파수 성분을 걸러내기 위하여 반드시 필터링을 필요로 한다. FIR 필터를 사용하여 모선 위치를 필터링한 값을 제어기의 목적위치로 설정하였다. 이러한 해석을 바탕으로 수치계산 알고리즘을 구축하여 모선의 여러 운동을 상정하여 시뮬레이션을 수행하였다. 이러한 수치계산 결과 필터링을 통해 고주파수 성분이 잘 걸러짐을 확인하였다. 또한 버퍼의 위치와 속도에 대한 제어를 통해서 버퍼를 허용범위(watch circle) 안에 머무르게 하면서 전체시스템을 안정화시킨다는 것을 밝혔다.

• 여성건강간호학회지
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• 제4권1호
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• pp.161-178
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• 1998

In spite of the great progress of the theory and skill of the Nursing Care & Medical area in relation to pregnancy, nurses in clinics face up to many challenges in maternity nursing care areas. The reason is that the mobility and mortality of mothers was sharply decreased and the unknown high-risk diseases of pregnancy woman in the past is made public. That's why it is difficult to meet the pregnancy woman in natural process from pregnancy to delivery in recently. Admission rooms are filled with high-risk pregnancy women. As a matter of fact, we have done nursing care into the surface symptoms and diseases of high-risk pregnancy women so far. We have been indifferent to a long period hospitalization, separation from family, and conflict of repeated examination. Therefore, it is widely spread to understand the emotional conflict experienced by high-risk pregnancy women and to need for nursing intervention to bring up about emotional support and the ability of perception in psychological crisis. Although the pregnancy woman judged in high-risk should carry out normal task of pregnancy, she have to be confronted with secondary risk situation. The health of self & fetus threatened by the risk situation could be decreased through care plan, but psychological stress increases. Therefore, the pregnancy brings into non-control state. It is important to ask that what the hospitalized pregnancy women in high-risk think of themselves status. Because misunderstanding or serious anxiety of themselves status put into mother and fetus in danger. And adaptation mode makes all the difference. I would like to consider how nurses could deal with this high-risk circumstances in the position of pregnancy woman on the basis of the above fact. This study uses phenomenological method to suggest the basis material for nurses to do nursing intervention in view of pregnancy woman. Because this method understands the nature of true life of pregnancy woman throughly. The phenomenological method is the sources to describe or explain affluently the process generated in confirmation areas and environment and is the application for readers to understand and recognize clinic reality and then apply this method to reasoning study place or other places. Specifically, the phenomenon study method, one of the phenomenological method, is applied. The use of that method is to describe and generalize the experience in environment exactly. The study of this study is as follows : Among 187 descriptive stamens from 8 study participants are classified into 42 theme cluster at the stage of the first analysis. Those theme is categorized into 8 sub-subjects such as anxiety of uncertainty, foreknowledge about risk circumstance, will power about overcome, unsettled feeling about hospital, relief, optimistic thought, family support, and indifferences. At the last stage of analysis, those things are categorized into 3 subjects. When high-risk pregnancy woman foretell the situation, they feel unsettlement about uncertainty and untrust feeling about hospital. But they are ease with family support and hospital support. On the other hand, they express indifferent 3-way structure response to the situation having will of overcome and exceeding optimistic thought. In those statements, the experience by pregnancy woman shows 3 respect subjects. 1. They are anxious of this situation and are in desperation and don't recognize their role to be carried out 2. They think of this situation as normal process of pregnancy and are not concerned that this can give themselves and fetus fatal damage. 3. The pregnancy women will never confront this situation. This study shows the pregnancy woman has anxiety and optimistic relief about the situation, and ignores and optimistic relief about the situation, and ignores many things. Therefore, nurses in clinic should give pregnancy woman knowledge and information about the high-risk and help them to deal with the situation spontaneously. High-risk pregnancy woman should have the care plan in respect of the right perception. And the nurse know that their support help out pregnancy woman overcome the crisis in this respect of the special nursing intervention.

• 대한화장품학회지
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• 제47권3호
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• pp.205-212
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• 2021

본 연구는 피부 섬유아세포에 자외선을 조사하거나 TNF-α를 처리하면 세포에 따라 MMP-1의 발현이 다르게 나타나는데, 이것이 MMP-1 프로모터의 다형성에 의해서 나타남을 밝히기 위해 수행되었다. 시판하는 23 종의 primary 섬유아세포에 대하여 MMP-1 프로모터의 -1607 부위의 유전형을 분석한 결과 6 개의 1G/1G 유전형, 10 개의 1G/2G 유전형, 7 개의 2G/2G 유전형을 가진 섬유아세포를 확인할 수 있었다. Hs68과 Detroit 551 세포주는 1G/2G 유전형을 가지는 것으로 확인되었다. 1G/1G 유전형은 TNF-α 처리에 의해 대조군에 비해 MMP-1이 2 배 높게 발현되었으며, 자외선에 의해서는 거의 발현되지 않았다. 1G/2G 유전형의 경우는 TNF-α 처리에 의해 MMP-1이 2.45 배 높게 발현되었으며, 자외선에 의해서는 1.4 배 MMP-1이 발현되었다. 2G/2G 유전형의 경우는 TNF-α 처리에 의해 MMP-1이 1.35 배 발현되었으며, 자외선에 의해서는 2.5 배로 높게 발현되었다. 즉 1G 유전형은 TNF-α에 의해, 2G 유전형은 자외선에 의해 발현이 유도되는 것으로 추정할 수 있으며, -1607 위치에 하나 더 삽입된 G에 의해서 Ets 전사인자가 결합할 수 있는 site가 만들어져서 MMP-1의 발현이 증가한다고 추정할 수 있으며, 피부 노화와 관련하여 섬유아세포에서는 이에 대한 연구가 전혀 진행되어 있지 않아서 향후 추가로 연구되어야 할 부분이다. 피부는 내인성 노화와 광노화의 영향을 동시에 받는 기관이므로, 피부 노화를 개선하기 위한 타겟으로 MMP-1의 발현을 분석할 경우에는 실험 조건에 적합한 유전형을 가지는 세포를 선택하여 연구를 진행하는 전략을 세워야 할 필요성이 대두된다.