• Molecules and Cells
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• v.42 no.9
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• pp.628-636
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• 2019

Altered genetic features in cancer cells lead to a high rate of aerobic glycolysis and metabolic reprogramming that is essential for increased cancer cell viability and rapid proliferation. Pyruvate kinase muscle (PKM) is a rate-limiting enzyme in the final step of glycolysis. Herein, we report that PKM is a potential therapeutic target in triple-negative breast cancer (TNBC) cells. We found that PKM1 or PKM2 is highly expressed in TNBC tissues or cells. Knockdown of PKM significantly suppressed cell proliferation and migration, and strongly reduced S phase and induced G2 phase cell cycle arrest by reducing phosphorylation of the CDC2 protein in TNBC cells. Additionally, knockdown of PKM significantly suppressed $NF-{\kappa}B$ (nuclear factor kappa-light-chain-enhancer of activated B cells) activity by reducing the phosphorylation of p65 at serine 536, and also decreased the expression of $NF-{\kappa}B$ target genes. Taken together, PKM is a potential target that may have therapeutic implications for TNBC cells.

• The Transactions of the Korea Information Processing Society
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• v.4 no.9
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• pp.2259-2268
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• 1997

Multistage interconnection networks(MINs) provide a high-bandwidth communication between processors and/or memory modules in a cost-effective way. In this paper, we propose a class of multipath MINs, called the Augmented Modified Delta(AMD) network, and analyze its performance and reliability. The salient features of the AMD network include fault-tolerant capability, modular structure, and high performance, which are essential for real-time parallel/distributed processing environments. The class of the AMD network retains well-known characteristics of the Kappa network, but it's design procedure is more systematic. Like Delta networks, all the AMD networks are topologically equivalent with each other.

• 한국전산유체공학회:학술대회논문집
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• 2006.10a
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• pp.181-184
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• 2006

High-speed flight vehicle have various cavities. The supersonic cavity flow is complicated due to vortices, flow separation and reattachment, shock and expansion waves. The general cavity flow phenomena include the formation and dissipation of vortices, which induce oscillation and noise. The oscillation and noise greatly affect flow control, chemical reaction, and heat transfer processes. The supersonic cavity' flow with high Reynolds number is characterized by the pressure oscillation due to turbulent shear layer, cavity geometry, and resonance phenomenon based on external flow conditions, The resonance phenomena can damage the structures around the cavity and negatively affect aerodynamic performance and stability. In the present study, we performed numerical analysis of cavities by applying the unsteady, compressible three dimensional Reynolds-Averaged Navier-Stokes(RANS) equations with the ${\kappa}-{\omega}$ turbulence model. The cavity model used for numerical calculation had a depth(D) of 15mm cavity aspect ratio(L/D) of 3, width to spanwise ratio(W/D) of 1.0 to 5.0. Based on the PSD(Power Spectral Density) and CSD(Cross Spectral Density) analysis of the pressure variation, the dominant frequency was analyized and compared with the results of Rossiter's Eq.

• Journal of computational fluids engineering
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• v.11 no.4 s.35
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• pp.62-66
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• 2006

High-speed flight vehicle have various cavities. The supersonic cavity flow is complicated due to vortices, flow separation, reattachment, shock waves and expansion waves. The general cavity flow phenomena includes the formation and dissipation of vortices, which induce oscillation and noise. The oscillation and noise greatly affect flow control, chemical reaction, and heat transfer processes. The supersonic cavity flow with high Reynolds number is characterized by the pressure oscillation due to turbulent shear layer, cavity geometry, and resonance phenomenon based on external flow conditions. The resonance phenomena can damage the structures around the cavity and negatively affect aerodynamic performance and stability. In the present study, we performed numerical analysis of cavities by applying the unsteady, compressible three dimensional Reynolds-Averaged Navier-Stokes(RANS) equations with the ${\kappa}-{\omega}$ turbulence model. The cavity model used for numerical calculation had a depth(D) of 15mm cavity aspect ratio (L/D) of 3, width to spanwise ratio(W/D) of 1.0 to 5.0. Based on the PSD(Power Spectral Density) and CSD(Cross Spectral Density) analysis of the pressure variation, the dominant frequency was analyzed and compared with the results of Rossiter's Eq.

• Journal of the Society of Naval Architects of Korea
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• v.45 no.6
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• pp.631-636
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• 2008

In this paper, numerical analysis based on the RANS equation and the Realizable ${\kappa}-{\varepsilon}$ turbulence model is carried out for flows around an axisymmetric body at three Reynolds numbers($1.22{\times}10^7$, $1.0{\times}10^8$, $1.5{\times}10^8$) and the numerical results are compared with experiments data. Computed velocity distributions agree well with experiments as the Reynolds number increases. Pressure distributions agree well with the results of the potential flow except the tail region but differ from experiments for the parallel middle body as well as tail region. Pressure gradients show a good agreement with those of potential flow and experiment except the tail region. Friction coefficients show that the numerical results generally are lower than the experimental results estimated from the measured velocity. The difference of friction coefficients between the calculation and the experiment increases with growing of a boundary layer.

• Wind and Structures
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• v.1 no.3
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• pp.271-284
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• 1998

This paper uses the numerical simulation to investigate the interference effect of 3-D turbulent flow around two high rise buildings in proximity at the different relative heights, gaps, and wind velocities. The computer program used to carry out the simulation is based on the control volume method and the SIMPLEST algorithm. The ${\kappa}-{\varepsilon}$ model was used to simulate turbulence effects. Since the contracted flow between two adjacent buildings enhances the strength of vortex shedding from the object building, the pressure coefficient on each side wall of the object building is generally increased by the presence of apposed building. The effect is increased as the relative height or the gap between the two buildings decreases. The velocity on the vertical center line between two buildings is about 1.4 to 1.5 times the upstream wind velocity.

• Korean Journal of Remote Sensing
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• v.25 no.3
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• pp.233-242
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• 2009

This paper presents two different types of supervised classifiers such as support vector machine (SVM) and spectral angle mapper (SAM). The Compact Airborne Spectrographic Imager (CASI) high resolution aerial image was classified with the above two classifier. The image was classified into eight land use /land cover classes. Accuracy assessment and Kappa statistics were estimated for SVM and SAM separately. The overall classification accuracy and Kappa statistics value of the SAM were 69.0% and 0.62 respectively, which were higher than those of SVM (62.5%, 0.54).

• The Korean Journal of Pharmacology
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• v.30 no.1
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• pp.1-9
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• 1994

It has been shown that there are several subtypes of ${\kappa}$ opioid receptor. We examined ligand binding profiles and the effects of various opioid agonists on high potassium-stimulated release of $[^3H]$ histamine. We have evaluated the properties of $non-{\mu},\;non-{\delta},$ binding of $[^3H]\;DIP\;([^3H]\;diprenorphine),$ anonselective opioid antagonist, in rat cortex membranes. Binding $to\;{\mu}\;and\;{\delta}$ sites was inhibited by the use of an excess of competing selective agonists (DAMGO, DPDPE) for these sites. (-) Ethylketocyclazocine (EKC), DIP and bremazocine inhibited $[^3H]$ DIP binding. However, arylacetamides (U69593 and U50488H) gave little inhibition Replacement of sodium by NMDG and the addition of guanine nucleotide influenced the inhibitory potency of (-) EKC, an agonist for {\kappa}_1-and-{\kappa}_2-binding site, but not of bremazocine. This result suggests that bremazocine can be an antagonist at this binding site. Also, we have examined the opioid modulation of $K^+(30mM)-induced\;[^3H]\;histamine$ release in rat frontal cortex slices labeled with $1-[^3H]\;histidine$. The $[^3H]\; histamine$ release from cortex slices was inhibited by EKC in a concentration-dependent manner. However, the ${\delta}$ receptor selective agonists, DPDPE and deltorphine II, ${\mu}$ receptor agonists, DAMGO and TAPS, ${\kappa}_1-agonists$, U69593 and U50488H, and ${\varepsilon}-agonist,\;{\beta}-endorphin,$ did not. The concentration-response curve of EKC was shifted to right in the presence of naloxone, nor-binaltorphimine and bremazocine, respectively. These results suggest that ${\kappa}_2$ opioid receptor regulates histamine release in the fromtal cortex of the rat.

• Journal of Preventive Medicine and Public Health
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• v.42 no.4
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• pp.223-230
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• 2009

Objectives : This study was conducted to validate self-reported smoking among high school students using urinary cotinine. Methods : A self report of smoking behavior was collected together with urine sample for cotinine analysis from 130 male and female students in two vocational high school students in November, 2007. Validity and agreement between self-reported smoking and urinary cotinine was analyzed with STATA 9.0 for different definitions of current smokers, and frequent and daily smokers. Urinary cotinine concentration was measured by the DRI Cotinine Assay for urine (Microgenics Corp., Fremont, CA) on Toshiba 200FR. The cut-off point of urinary cotinine was 50 ng/dl. Results : The concentrations of urinary cotinine were significantly different according to the frequency and amount of smoking. Sensitivity and specificity was 90.9% and 91.8% respectively, and the Cohen s kappa value was 0.787 among the current smokers who smoked at least one day during one month preceding the survey. The comparable high sensitivity, specificity, and kappa value were shown also among the other definitions of current smokers, that is, subjective smokers, and weekly smokers. Conclusions : The results showed the high validity of self-reported smoking among high school students. However, due to the small sample size and limitation of the participants, it is cautious to generalize the results to overall high school students.

• The Journal of Korean Academy of Sensory Integration
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• v.14 no.1
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• pp.1-8
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• 2016

Objective : The purpose of this study was to determine the reliability of Test of Playfulness (ToP) in Attention Deficit Hyperactivity Disorder (ADHD) children. Methods : Twenty ADHD children participated in this study. To determine inter-rater reliability, two raters was evaluated by Test of Playfulness scores and analyzed Kappa coefficient and total agreement and intraclass correlation coefficient (ICC). Results : Total agreement was the range of 75%~95% and the Kappa coefficients was the range of. 45~1.00. Analyzing inter-rater reliability by ICC showed very high reliability: Intrinsic motivation was .96, internal control was .98, suspension of reality was .90, framing was .98, total score was .99. Conclusion : A reliability of Test of Playflness of children with ADHD showed that high. On the basis of this study, it will provide availability for clinical evaluation of children with ADHD, and basic study on the play of children with ADHD.