• Asian-Australasian Journal of Animal Sciences
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• v.23 no.11
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• pp.1510-1518
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• 2010

The study was conducted to evaluate the effect of lighting schedule and nutrient density on growth performance, carcass traits and meat quality of broiler chickens. A total of 576 day old Arbor Acre male chickens was used with a $4{\times}2$ factorial arrangement. The four lighting schedules were continuous (23 L:1 D, CL), 20 L:4 D (12 L:2 D:8 L:2 D), 16 L:8 D (12 L:3 D:2 L:3 D: 2 L:2 D) and 12 L:12 D (9 L:3 D:1 L:3 D:1 L:3 D:1 L:3 D) and provided by incandescent bulbs. The two nutrient densities were high (H, starter diet: 13.39 MJ/kg apparent metabolisable energy (AME), 23.00% crude protein (CP); finisher diet: 13.39 MJ AME/kg, 19.70% CP) and low energy and protein level (L, starter diet: 12.03 MJ AME/kg, 20.80% CP; finisher diet: 12.14 MJ AME/kg, 18.30% CP). Houses with dark curtains and solid sidewalls were used. Chickens were randomly allocated to the 8 treatments with each treatment comprising 6 replicates of 12 chickens. Feed and water were available ad libitum. Lighting schedules showed no difference (p>0.05) in growth performance at the end of the experiment. 12 L:12 D significantly reduced (p<0.05) the concentration of malondialdehyde (MDA) compared to 23 L:1 D treatment. Intermittent lighting (IL) schedules produced higher protein content (p<0.001) in breast meat. Birds on high density diets had higher body weight (BW), feed intake (FI) (p<0.001), and feed conversion ratio (FCR) (p<0.001) throughout the experiment with the exception of 36 to 42 d. High nutrient density increased (p<0.05) abdominal fat, decreased (p<0.05) the moisture loss of meat, and reduced percentage of wings and legs. There was a significant lighting schedule${\times}$diet interaction (p<0.001) on FCR for days 8 to 14 and 15 to 21. Results indicated that IL can give similar growth performance in comparison with CL, meanwhile with positive effects on meat quality by increasing protein content and decreasing the concentration of MDA. High nutrient density resulted in greater growth performance.

• Journal of Aquaculture
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• v.18 no.3
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• pp.160-166
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• 2005

Two feeding trials were carried out to investigate apparent nutrient digestibility of flounder fed experimental diets containing different levels of macro-nutrients by satiation feeding rate (Exp-1) and feeding frequency (Exp-2). Triplicate groups of fish averaging 280 g were fed three experimental diets which contained different levels of carbohydrate, protein and lipid by two feeding regimes (satiation and 80% satiation) and four feeding frequencies (three meals a day, two meals a day, one meal a day and one meal every two days). Feces were collected using a fecal collection column attached to fish rearing tanks for 6 weeks. Apparent digestibilities of dry matter, protein, lipid, energy and carbohydrate were not affected by feeding satiation rate in Exp-1. Apparent protein digestibility was not affected by feeding frequency, whereas affected by dietary composition in Exp-2. Apparent protein digestibility of fish fed a high-protein diet showed a tendency to become higher compared to that of fish fed high-carbohydrate diet and high-lipid diet at the same feeding frequency. Apparent lipid digestibility was not affected by dietary composition, however, affected by feeding frequency. Apparent digestibilities of energy and carbohydrate were affected by both dietary composition and feeding frequency. Apparent digestibities of energy and carbohydrate in fish fed the high-protein diet showed a tendency to become higher compared to that of fish fed the high-carbohydrate diet and high-lipid diet at the same feeding frequency. Apparent digestibities of energy and carbohydrate tended to decrease with increasing of feeding frequency at the same dietary composition.

• The Korea Journal of Herbology
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• v.28 no.1
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• pp.91-96
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• 2013

Objectives : Obesity is a chronic metabolic disease caused by disorder of energy balance and lipid metabolism. This study was conducted by histopathology and histomorphometry to investigate the anti-obesity effects of mixed water extract of Plantaginis Semen & Poria (CJB) on liver, epididymal fat pads and pancreas zymogen granules in obese rats induced with high fat diet. Method : Male Sprague-Dawley rats to be divided four groups were fed into four different treatments: normal (NOR) diet, high-fat (HF) diet, HF diet+CJB (100 mg/kg and 300 mg/kg, P.O.) for 8 weeks. The weekly body weights were measured in four experimental groups, respectively. Also histopathological and histomorphometrical changes of liver, epididymal fat pads and pancreas zymogen granules were observed in normal control and obese rats, respectively. Results : Adminstration of CJB significantly reduced body weights compared to those of HF group for experimental period. After 8 weeks, liver weights in the CJB groups were lower than those of HF group. In addition, HF diet related steatohepatitis, adipocyte hypertrophy, exocrine disturbances (decreases of pancreatic zymogen granules) were also dose-dependently inhibited by treatment of test material, CJB 100 and CJB 300 as compared with HF group, respectively. Conclusion : Based on the results, it is considered that CJB will be showed hepatoprotective and anti-obese effects, may be directly and/or indirectly mediated by pancreatic zymogen granules because they dose-dependently inhibited steatohepatitis, hypertrophy of adipocytes and decreases of pancreatic zymogen granules induced by HF diet supply, respectively.

• Nutrition Research and Practice
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• v.8 no.6
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• pp.625-631
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• 2014

BACKGROUND/OBJECTIVES: The main objective of this study was to evaluate the effects of a high cholesterol (HC) dietary challenge on cholesterol tissue accumulation, inflammation, adipocyte differentiation, and macrophage infiltration in guinea pigs. A second objective was to assess whether macronutrient manipulation would reverse these metabolic alterations. MATERIALS/METHODS: Male Hartley guinea pigs (10/group) were assigned to either low cholesterol (LC) (0.04g/100g) or high cholesterol (HC) (0.25g/100g) diets for six weeks. For the second experiment, 20 guinea pigs were fed the HC diet for six weeks and then assigned to either a low carbohydrate (CHO) diet (L-CHO) (10% energy from CHO) or a high CHO diet (H-CHO) (54% CHO) for an additional six weeks. RESULTS: Higher concentrations of total (P < 0.005) and free (P < 0.05) cholesterol were observed in both adipose tissue and aortas of guinea pigs fed the HC compared to those in the LC group. In addition, higher concentrations of pro-inflammatory cytokines in the adipose tissue (P < 0.005) and lower concentrations of anti-inflammatory interleukin (IL)-10 were observed in the HC group (P < 0.05) compared to the LC group. Of particular interest, adipocytes in the HC group were smaller in size (P < 0.05) and showed increased macrophage infiltration compared to the LC group. When compared to the H-CHO group, lower concentrations of cholesterol in both adipose and aortas as well as lower concentrations of inflammatory cytokines in adipose tissue were observed in the L-CHO group (P < 0.05). In addition, guinea pigs fed the L-CHO exhibited larger adipose cells and lower macrophage infiltration compared to the H-CHO group. CONCLUSIONS: The results of this study strongly suggest that HC induces metabolic dysregulation associated with inflammation in adipose tissue and that L-CHO is more effective than H-CHO in attenuating these detrimental effects.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.823-830
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• 2005

This study was undertaken to evaluate the effect of bacteria-derived $\beta$-glucan fiber on serum lipids, adiposity and uncoupling protein (UCP) expression in rats. In order to induce obesity, Sprague-Dawley weanling male rats were allowed free access to AIN-76A diet until 4 weeks of age, and fed high-fat diet (beef tallow, $40\%$ of calories as fat) for 6 weeks until 10 weeks of age. Rats were then fed with $0\%$ thigh- fat control group), $1\%$, or $5\%$ bacterial ~-glucan supplemented high-fat diets (w/w) for another 6 weeks. For comparison, normal control group was fed with AIN-76 diet $11.7\%$ fat). Supplementation with bacterial $\beta$-glucan resulted in a significant reduction of high-fat-induced white fat (i.e., visceral and peritoneal fat) development, adipocyte hypertrophy, and development of hyperinsulinemia and hyperleptinemia. Serum triglyceride, total cholesterol, and free fatty acid levels were greatly reduced, but, HDL-cholesterol concentrations were increased by bacterial $\beta$-glucan supplementation. Serum leptin level was lower in the $\beta$-glucan groups than in the high-fat group. The expression of UCPs (UCP1, UCP2, and UCP3) in brown adipose tissue (BAT) were significantly increased by $5\%$ bacterial $\beta$-glucan-containing diet. This study suggests that the anti-obesity effect of $5\%$ bacterial $\beta$-glucan is attributed to upregulation of UCPs and inefficient energy utilization.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.5
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• pp.580-587
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• 2010

An experiment was conducted to evaluate the effect of dietary water-soluble carbohydrate extract from Renga renga lily (Arthropodium cirratum) and a commercial product, $Frutafit^{{\circledR}}$ (both fructans) on the performance, organ weights, ileal digestibility and gut morphology of male Cobb broiler chickens. There were six treatment groups: a negative control with no supplements, a positive control supplemented with 45 ppm Zn-bacitracin, and four test diets each supplemented with Renga renga lily extract or Frutafit at 5 or 10 g/kg diet. Supplementation with low levels of Renga renga lily extract and Frutafit in the diet did not affect productive parameters, whereas the inclusion of a high level of Frutafit had a negative effect on BWG and FI compared with birds fed the negative control diet. The addition of an antibiotic to the diet significantly improved (p<0.05) the BWG and FCR of broilers. Apparent ileal digestibility of dry matter, starch, protein and fat was not affected (p>0.05) by supplementation with both levels of lily extract and the low level of Frutafit. The apparent ileal digestibility of dry matter, protein and fat was decreased (p<0.05) by the high level of Frutafit. The apparent metabolisable energy (AME) of the diets fed the high level of Frutafit was approximately 0.2 MJ/kg DM lower than that of the negative control group. The addition of Zn-bacitracin increased (p<0.05) the apparent ileal digestibility of fat. The relative weight of the liver was higher (p<0.05) in broilers supplemented with the high level of Frutafit than for negative control birds at 14 and 35 d of age. Feeding Renga renga lily extract or Frutafit had no effect on the gut morphology of birds on d 14 and 35. It can be concluded that dietary inclusion of fructans from the two sources used in this study affected broiler performance differently and in a dose-dependent manner.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.855-860
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• 2005

This study was designed to test the effects of changes in energy and protein intake levels on the maternal milk amino acid concentrations over the short-term in lactating mares. Three lactating mares were enrolled for the study 7 weeks after parturition. A low-energy and low-protein diet (LEP) was administered during the first week of the study, followed by administration of a high-energy and high-protein diet (HEP), again for a week (day 1 to day 7), and milk was sampled thrice daily at intervals of 8 h during the study period. The mean amino acid concentrations in the maternal milk, except for those of proline, serine and valine, were significantly higher in the HEP feeding period than during the LEP feeding period (p<0.05). The sum of the concentrations of all the amino acids (TAA) in the maternal milk samples during the HEP and LEP feeding periods was 1,644.9${\pm}$26.9 and 1,542.3${\pm}$36.0 mg/100 g, respectively, the difference between the two was not significant. When the ratio of each amino acid concentration to the TAA in the maternal milk was analyzed, there were significant differences between the HEP and LEP feeding periods for all amino acids, except glycine, serine, alanine and histidine. It was found that the concentrations of glutamic acid+glutamine, serine, threonine, arginine and valine were significantly higher (p<0.05) on day 1 than on day 7 during the LEP feeding period, and there were no such differences during the HEP feeding period. In regard to the effects of changes in the energy and protein intake levels in lactating mares, no changes in milk amino acid concentrations were found following administration of HEP for a week, whereas 7 days of administration of LEP was associated with a decrease in the amino acid concentrations.

• The Korean Journal of Community Living Science
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• v.14 no.3
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• pp.43-51
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• 2003

This study examined to find out the relationship between nutritional knowledge and food habit of the students majoring food & nutrition(218 students) and the students majoring Korean oriental medicine(286 students). The results of this study are as follows; 1)The intake of vegetable and dairy products is high for the students majoring food & nutrition(p＜0.01), and the intake of instant food is low for the students majoring Korean oriental medicine(p＜0.01). 2)In the value of diet, the ratio which acknowledges the importance of the relationship between the diet and the nutrients is high for the students majoring food & nutrition (p＜0.01), and the ratio which acknowledges the diet as the way to remove hunger or to enjoy eating is high for the students majoring Korean oriental medicine(p＜0.01). The ratio of the students who acknowledge taste, nutrition and preference as the important factors in selecting foods is high for the students majoring food & nutrition(p＜0.01). The ratio of regularity in each meal, consuming time, and the food quantity is high for the students majoring Korean oriental medicine(p＜0.01). 3)The total score of 20 inquiring items about nutritional knowledge is high for the students majoring food & nutrition(p<0.01), and especially the mark is high in such items inquiring the relationship between animal fat and nutrition(p<0.01), the relationship between water and obesity(p<0.05), the relationship between cholesterol and food(p<0.01), the recommended energy quantity(p<0.05), and the vitamin B group (P<0.01). According to this study, it is hard to determine that there is inevitable positive relationship between diet habits and nutritional knowledge. The students majoring food and nutrition know better than the students majoring Korean oriental medicine about the relationship between diet, health and nutrients however they can not practice what they blow when selecting foods. On the other hand the students majoring Korean oriental medicine show lower marks in food habits and nutritional knowledge than those of the students majoring food and nutrition; however, they show higher marks in practicing regular diet. According to this result it is necessary to increase the subjects related nutrition in the curriculum not only lot the students majoring food & nutrition to practice their nutritional knowledge in their actual life but also for the students majoring Korean oriental medicine to obtain correct nutritional knowledge and information.

• Journal of Fisheries and Marine Sciences Education
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• v.29 no.3
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• pp.888-898
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• 2017

Tenebrio molitor larvae, also known as yellow mealworms (MW), are rich in protein and lipid and can serve as a potential alternative protein and energy source in commercial aquafeeds. Therefore, this study attempts to evaluate the effects of different drying methods on the nutritional value of MW meal. For this, live MW were cold-anaesthetized before being subjected to three different types of drying methods, including freeze-drying, oven-drying at $60^{\circ}C$ and air-drying at room temperature for three days, and compared for proximate composition and energy content. An in-vivo digestibility test was then conducted to evaluate the nutrient digestibility of MW meal in diets for rockfish, Sebastes schlegeli. A test diet was prepared by mixing the MW meal with a reference diet (Ref) in a 30:70 ratio with chromium oxide as an inert marker at the inclusion level of 0.5%. Rockfish with mean body weight of 150 g were stocked into a fecal collection system equipped with fiberglass tanks of 400 L capacity. Each group of fish was fed one of the experimental diets to apparent satiation for 4 weeks. The results of the proximate analysis showed that drying methods had no significant effect on crude protein, crude lipid, ash and energy contents of MW. Despite being a rich source of protein and lipid, MW meal was deficient in certain amino acids, particularly methionine, and highly unsaturated fatty acids, particularly 22:6n-3 (DHA) and 20:5n-3 (EPA). MW meal showed high digestibility values for protein (93%), lipid (97%) and energy (88%). These results may indicate that MW meal is a nutritious and acceptable feed ingredient, with comparable digestibility values to conventional animal and plant feedstuffs such as fish meal and soybean meal, in practical diet for rockfish at grower stage.

• Korean Journal of Agricultural Science
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• v.49 no.1
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• pp.45-50
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• 2022

The aim of this study is to evaluate the effects of supplementation of low-energy diets with PROSOL^® (sodium stearoyl-2-lactylate) as an emulsifier on the growth performance and nutrient digestibility of growing pigs. A total of 120 crossbred ([Landrace × Yorkshire] × Duroc) growing pigs with an average initial body weight of 23.80 ± 4.87 kg were used in a 56-day feeding trial and were sorted into three dietary treatments. Each treatment utilized eight replications with five pigs per pen. The dietary treatments were as follows: PC (high, net energy diet [NE] = 2,488 kcal·kg^-1 [weeks 1 to 4] and NE = 2,477 kcal·kg^-1 [weeks 5 to 8]); NC (low, NE = 2,472 kcal·kg^-1 [weeks 1 to 4] and NE = 2,468 kcal·kg^-1 [weeks 5 to 8]); NC1 (NC + 0.05% PROSOL^® emulsifier). Growth performance outcomes were measured at the initial point and at weeks 4 and 8 while nutrient digestibility parameters were measured at weeks 4 and 8. From weeks 1 - 8, body weight and the average daily gain of the NC1 group pigs showed significant increases (p < 0.05) compared to those of the PC and NC treatment groups. At the end of the trail, pigs fed a diet containing low net energy with 0.05% of the emulsifier showed reduced feed conversion ratio (FCR) levels. However, the average daily feed intake and nutrient digestibility of dry matter and nitrogen remained unaffected throughout the experiment. In summary, the addition of a low-net-energy diet with 0.05% sodium stearoyl-2-lactylate as an emulsifier enhanced the body weight and daily gain with no adverse effects on nutrient digestibility in growing pigs.