• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.143-153
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• 1996

To improve the efficiency of nuclear transplantation in the rabbit, this study were evaluated the influence of celly cycle of donor nuclei on the in vitro developmental potential in the nuclear transplant embryos. The embryos of 16-cell stage were collected from the mated does at 48h post-hCG injection and they were synchronized to G1 phase of 32-cell stage. Synchronization of the cell cylce of blastomeres were induced, first, using an microtubules polymerization inhibitor, 0.5$\mu\textrm{g}$/ml colcemid for 10h to arrest blastomeres in metaphase, and secondly, using a DNA synthesis inhibitor, 0.1$\mu\textrm{g}$/ml aphidicolin for 1.5 to 2h to cleave to 32-cell stage and arrest them in G1 phase. The separated G1 phase blastomeres of 32-cell stage were injectied into enucleated recipient cytoplasms by micromanipulation. After culture until 20h post-hCG injection, the nuclear transplant oocytes were electrofused and activated by electrical stimulation. The nuclear transplant embryos were co-cultured for 120h. In vitro cultured embryos were monitored every 24h to assess for development rate. After in vitro cultue for 120h, the nuclear transplant embryos developed to blastocyst stage were stained with Hoechst 33342 dye for counting the number of blastomeres under a fluorescence microscopy. The cleavage rate of blastomeres from 16-cell stage stage rabbit embryos treated with colcemid for 10h or aphidicolin for 6h following colcemid for 10h were not significantly different. The electrofusion rate was similar by high in S and G1 phase donor nuclei as 80.6 and 79.1%, respectively. However, the nuclear transplant embryos using G1 phase donor nuclei were developed to blastocyst at high rate(60.3%) than those using S phase donor nuclei(26.0%). Moreover, the mean blastocyst stage were increased significantly(P<0.05) with the G1 phase donor nuclei(176.6 cells and 1.50 cycles), as compared with the S phase donor nuclei(136.6 cells and 1.42 cycles). These results show that the blastomeres of G1 phase were more successful as donor nuclei in the nuclear transplant procedure, compared with S phase.

• Journal of Radiation Protection and Research
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• v.31 no.4
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• pp.173-179
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• 2006

The Monte Carlo simulation code CEARPPU has been developed and updated to provide pulse pile-up correction spectra for high counting rate cases. For neutron activation analysis, CEARPPU correction spectra were used in library least-squares method to give better isotopic activity results than the convention library least-squares fitting with uncorrected spectra.

• Journal of Korea Multimedia Society
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• v.21 no.9
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• pp.1076-1089
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• 2018

Respiration is the process of moving air into and out of the lung. Respiration changes the temperature in the chamber while exchanging energy. Especially the temperature of the face. Respiration monitoring using an infrared camera measures the temperature change caused by breathing. The conventional method assumes that motion is not considered and measures respiration. These assumptions can not accurately measure the respiration rate when breathing moves. In addition, the respiration rate measurement is performed by counting the number of peaks of the breathing waveform by displaying the position of the peak in a specific window, and there is a disadvantage that the breathing rate can not be measured accurately. In this paper, we use KLT tracking and block matching to calibrate limited weak movements during breathing and extract respiration waveform. In order to increase the accuracy of the respiration rate, the position of the peak used in the breath calculation is calculated by converting from a single point to a high resolution. Through this process, the respiration signal could be extracted even in weak motion, and the respiration rate could be measured robustly even in various time windows.

• Journal of Advanced Marine Engineering and Technology
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• v.31 no.2
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• pp.152-158
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• 2007

Most of experimental visualizations and numerical results on the flow field separated form a leading edge around an unsteady foil show a continuous streakline from the leading edge and large reverse flow between the streakline and the suction surface. However, they have not exactly clarified yet the dynamic behavior of vortices separated from the leading edge because separation around an unsteady foil is very complicated phenomenon due to many parameters. In the present study the flow fields around pitching foils have been visualized by using a Schlieren method with a high speed camera in a wind tunnel at low Reynolds number regions. It has been observed that small vortices are shed discretely from the leading and trailing edge and that they stand in line on the integrated streakline of separation shear layer. By counting vortices in the VTR frames it was clarified that the number of vortex shedding from the leading and trailing edge during one pitching cycle strongly depends on the non-dimensional pitching rate. Futhermore the vortices moving up to the leading edge on the suction surface of the pitching foil are visualized. They play an important role to balance the number of vortex shedding from both edges.

• Proceedings of the KSME Conference
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• 2000.11a
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• pp.135-140
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• 2000

The characteristics of mechanical behavior were estimated for Ti-6Al-4V alloy with four kinds of microstructure prepared with heat treatments. For this study, impact test, tensile test and fatigue crack growth test were performed, and then compared mechanical properties on the four microstructures. Furthermore, for quantitative evaluation, fractal dimensions of crack pass were obtained using the box counting method. The main results obtained are summarized as follows. (1) The microstructures exhibited equiaxed microstructure, bimodal-microstructure and lamellar microstructure by heat treatment. (2) The impact absorbed energy and elongation is superior in the bimodal-microstructure, and the hardness and tensile strength are superior in the lamellar microstructure. (3) The fatigue crack growth rate is similar to all microstructures in the low ${\Delta}K$ region. The fatigue crack growth rate of equiaxed microstructure is fastest, and that of lamellar microstructure is lowest in the high ${\Delta}K$ region. (4) The fractal dimension D of lamellar microstructure is higher then that of the equiaxed microstructure and bimodal microstructure.

• Journal of Animal Science and Technology
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• v.65 no.6
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• pp.1254-1269
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• 2023

This study aims to predict the change in corn share according to the grazing of 20 gestational sows in a mature corn field by taking images with a camera-equipped unmanned air vehicle (UAV). Deep learning based on convolutional neural networks (CNNs) has been verified for its performance in various areas. It has also demonstrated high recognition accuracy and detection time in agricultural applications such as pest and disease diagnosis and prediction. A large amount of data is required to train CNNs effectively. Still, since UAVs capture only a limited number of images, we propose a data augmentation method that can effectively increase data. And most occupancy prediction predicts occupancy by designing a CNN-based object detector for an image and counting the number of recognized objects or calculating the number of pixels occupied by an object. These methods require complex occupancy rate calculations; the accuracy depends on whether the object features of interest are visible in the image. However, in this study, CNN is not approached as a corn object detection and classification problem but as a function approximation and regression problem so that the occupancy rate of corn objects in an image can be represented as the CNN output. The proposed method effectively estimates occupancy for a limited number of cornfield photos, shows excellent prediction accuracy, and confirms the potential and scalability of deep learning.

• Journal of the Institute of Electronics and Information Engineers
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• v.54 no.3
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• pp.70-77
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• 2017

Recently, Internet of Things (IoT) and deep learning techniques have affected video surveillance systems in various ways. The surveillance features that perform detection, tracking, and classification of specific objects in Closed Circuit Television (CCTV) video are becoming more intelligent. This paper presents real-time algorithm that can run in a PC environment using only a low power CPU. Traditional tracking algorithms combine background modeling using the Gaussian Mixture Model (GMM), Hungarian algorithm, and a Kalman filter; they have relatively low complexity but high detection errors. To supplement this, deep learning technology was used, which can be trained from a large amounts of data. In particular, an SRGB(Sequential RGB)-3 Layer CNN was used on tracked objects to emphasize the features of moving people. Performance evaluation comparing the proposed algorithm with existing ones using HOG and SVM showed move-in and move-out error rate reductions by 7.6 % and 9.0 %, respectively.

• Nuclear Engineering and Technology
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• v.52 no.6
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• pp.1282-1288
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• 2020

In the first study, the Radon emanation and radiological hazards associated with radionuclides in soil samples, collected from 9 various date palm farms located in 3 different districts in Saudi Arabia were determined through a high purity Germanium (HPGe) gamma-ray spectrometer. The estimated average values of Radon emanation coefficient and Radon mass exhalation rate for soil samples were 0.535 ± 0.016 and 50.063 ± 7.901 mBqkg^-1h^-1, respectively. The annual effective dose of radionuclides in all sampling locations was found to be lower than UNSCEAR's recommended level of 0.07 mSvy^-1 for soil in an outdoor environment. In the secondary study, gross α and gross β activities in soil and date palm pits samples were measured by a low background α/β counting system. Average values of gross α and gross β activities in soil and date palm pits samples were 5.761 ± 0.360 Bqkg^-1, 38.219 ± 8.619 Bqkg^-1 and 0.556 ± 0.142 Bqkg^-1, 24.266 ± 1.711 Bqkg^-1, respectively.

• Journal of the Korean Society for Precision Engineering
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• v.19 no.9
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• pp.172-178
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• 2002

One of the ever-increasing demands on the performances of heterodyne interferometers is to improve the measurement resolution, of which current state -of-the-art reaches the region of sub-nanometers. So far, the demand has been met by increasing the clock speed that drives the electronics involved fur the phase measurement of the Doppler shift, but its further advance is being hampered by the technological limit of modem electronics. To cope with the problem, in this investigation, we propose a new scheme of phase -measuring electronics that reduces the measurement resolution without further increase in clock speed. Our scheme adopts a super-heterodyne technique that lowers the original beat frequency to a level of 1 MHz by mixing it with a stable reference signal generated from a special phase- locked-loop. The technique enables us to measure the phase of Doppler shift with a resolution of 1.58 nanometer at a sampling rate of 1 MHz. To avoid the undesirable decrease in the maximum measurable speed caused by the lowered beat frequency, a special form of frequency up-down counting technique is combined with the super-heterodyning. This allows performing required phase unwrapping simply by using programmable digital gates without 2n ambiguities up to the maximum velocity guaranteed by the original beat frequency.

• Parasites, Hosts and Diseases
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• v.28 no.1
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• pp.63-67
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• 1990

This study was performed to evaluate the status of intestinal parasitic infections among the inhabitants in two islands (Chu-do and Doomi-do) of Tongyeong.gun, Kyeongsangnam-do (Province), from August to September, 1989. A total of 189 stool specimens was collected from the inhabitants of 3 villages and examined by Kato's cellophane thick smear and formalin.ether sedimentation techniques. Stoll's dilution egg counting technique was done for the quantitative examination of helminth eggs. The overall Positive rate of intestinal Parasites was 30.2%. The egg Positive rate of Ascaris lumbricoides was 2.1%, that of Trichuris trichiura 24.3%, hookworm 2.1%, Trichostrongylus orientazis 0.5%, Czonorchis sinensis 1.1%, heterophyid 1.6%, and Taenia species 2.6%. The cyst positive rate of Giardia lamblia was 1.6% and that of Entamoeba soli 0.5%. In T. trichiura infection, the egg Positive rate of females (29.9%) was much higher than that of males (17.2%). Among the age groups, 10~19 year group showed the highest inflection rate, 32.4%. It was revealed that the prevalence of intestinal parasitic infections among the inhabitants of remote islands should be still high in comparison with ever-reported ones in urban or rural areas.