• Journal of the Korean Society for Heat Treatment
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• v.24 no.2
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• pp.92-98
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• 2011

The influence of the cooling condition after solution treatment on the high temperature fatigue resistance of 23Cr-26Ni heat resistant steel was investigated. Two different cooling conditions were applied to the steel after solution treatment at $1200^{\circ}C$ for 3 hours. One specimen was water quenched immediately after the solution treatment. The other one was furnace cooled at a rate of $0.5^{\circ}C/min$ down to $750^{\circ}C$ after the solution treatment. Then, both specimens were aged at $750^{\circ}C$ for 5 hours. Under two different heat treatment conditions, the low cycle fatigue (LCF) test was performed at $600^{\circ}C$ and room temperature (RT). Only cyclic hardening continued from the beginning until fracture at all strain amplitudes during LCF at $600^{\circ}C$. This phenomenon was attributed to the increase in the dislocation density due to cyclic deformation, which resulted in the interaction between the newly created dislocations and precipitates. Cyclic hardening followed by saturation and cyclic softening was observed at RT. Cyclic softening was attributed to the dislocation annihilation rate exceeding the dislocation generation rate. Other probable factor for cyclic softening was some cavities formed around grain boundaries after 20 cycles. WQ and FC have a similar LCF behavior at RT and $600^{\circ}C$ as shown in the cyclic stress response curves.

• Journal of Animal Science and Technology
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• v.64 no.6
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• pp.1144-1172
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• 2022

Salmonella enterica serovar Typhimurium isolate HJL777 is a virulent bacterial strain in pigs. The high rate of salmonella infection are at high risk of non-typhoidal salmonella gastroenteritis development. Salmonellosis is most common in young pigs. We investigated changes in gut microbiota and biological function in piglets infected with salmonella via analysis of rectal fecal metagenome and intestinal transcriptome using 16S rRNA and RNA sequencing. We identified a decrease in Bacteroides and increase in harmful bacteria such as Spirochaetes and Proteobacteria by microbial community analysis. We predicted that reduction of Bacteroides by salmonella infection causes proliferation of salmonella and harmful bacteria that can cause an intestinal inflammatory response. Functional profiling of microbial communities in piglets with salmonella infection showed increasing lipid metabolism associated with proliferation of harmful bacteria and inflammatory responses. Transcriptome analysis identified 31 differentially expressed genes. Using gene ontology and Innate Immune Database analysis, we identified that BGN, DCN, ZFPM2 and BPI genes were involved in extracellular and immune mechanisms, specifically salmonella adhesion to host cells and inflammatory responses during infection. We confirmed alterations in gut microbiota and biological function during salmonella infection in piglets. Our findings will help prevent disease and improve productivity in the swine industry.

• KSBB Journal
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• v.25 no.2
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• pp.142-154
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• 2010

The protein-bound innerpolysaccharides (IPS) produced by suspended mycelial cultures of Inonotus obliquus have promising potentials as an effective antidiabetic as well as an immunostimulating agents. To enhance IPS production, intensive strain improvement process should be carried out using large amount of UV-mutated protoplasts. During the whole strain-screening process, the stage of solid growth-culture was found to be the most time-requiring step, thus preventing rapid screening of high-yielding producers. In order to reduce the cell growth period in the solid growth-stage, therefore, solid growth-medium was optimized using the statistical methods such as (i) Plackett-Burman and fractional factorial designs (FFD) for selecting positive medium components, and (ii) steepest ascent (SAM) and response surface (RSM) methods for determining optimum concentrations of the selected components. By adopting the medium composition recommended by the SAM experiment, significantly higher growth rate was obtained in the solid growth-cultures, as represented by about 41% larger diameter of the cell growth circle and higher mycelial density. Sequential optimization process performed using the RSM experiments finally recommended the medium composition as follows: glucose 25.61g/L, brown rice 12.53 g/L, soytone peptone 12.53 g/L, $MgSO_4$ 5.53 g/L, and agar 20 g/L. It should be noted that this composition was almost similar to the medium combinations determined by the SAM experiment, demonstrating that the SAM was very helpful in finding out the final optimum concentrations. Through the use of this optimized medium, the period for the solid growth-culture could be successfully reduced to about 8 days from the previous 15~20 days, thus enabling large and mass screening of high producers in a relatively short period.

• Journal of Crop Science and Biotechnology
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• v.10 no.3
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• pp.167-174
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• 2007

Spongy Alphonso mangoes were found to be infected with Staphylococcus bacteria. A Gram positive Staphylococcus strain was isolated from spongy pulp and identified from CABI Bioscience, UK, by partial 16S rDNA sequence analysis and by morphological and biochemical characterization through IMTECH, Chandigarh, India. Although identification by both of these methods indicated the organism belonged to same genus, different species names were given. Changes in total phenolics, reducing, and non-reducing sugars, respiration rate, total carotenoids, peroxidase(POX), and catalase activities were monitored during ripening of these fruits. The climacteric rise in spongy fruits was marked by an increase in respiration rate and a decrease in sugar content. Total phenolics content increased in spongy fruits as compared to ripe non-spongy fruits. Development of corky white tissue in spongy fruits was associated with about a 2.5-fold reduction in total carotenoids and a concomitant increase in lipoxygenase-mediated, $\beta$-carotene co-oxidation. A marked decrease in soluble protein content and about a 1.5-fold increase in POX activity was observed. Maximum POX activity was confined to 50-70%$(NH_4)_2SO_4$ fraction. The intense dark bands visible after POX specific substrate staining of the Native gel indicated a high expression of isoenzymes of POX in spongy fruits. Similarly, changes in levels of catalase activity were also observed in spongy fruits. The results suggest that infection of Alphonso mangoes with Staphylococcus bacteria affects the normal ripening processes of the fruit interfering with the carbohydrate and carotenoid metabolism. Also, the studies indicate the expression of POX and catalase enzymes as a plant defense response to microbial invasion.

• Fashion & Textile Research Journal
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• v.2 no.3
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• pp.265-271
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• 2000

Heat stress results in fatigue, a decline in strength, alertness., and mental capacity. The problem is compounded when high humidity exists. To help relieve worker heat stress, many types of cooling units are marketed. While workers may experience some cooling, critical body core temperatures often continue to elevate. This study was designed to find the effects of three kinds of cooling vest with portable frozen gel strips on thermophysiological parameters and on temperature and humidity within clothing. The heart rate, rectal, and skin temperature as well as sweat rate and clothing microclimate were measured during 80 min in 5 healthy males. Inquiries were also made into the subjective rating thermal, humidity comfort, and fatigue sensations. The main findings in our experiments are as follows: (a) Physiological parameters such as rectal temperature was the lowest in garb A1, intermediate in garb A, and the highest in garb A2 throughout the experiment. And mean skin temperature was the lowest in garb A, intermediate in garb A1, and the highest in garb A2; (b) Temperature and humidity within clothing (back) were garb in Al, intermediate in garb A, and the highest in garb A2. But the temperature and humidity within clothing (chest) were garb in A, intermediate in garb A1, and the highest in garb A2; (c) Most participants (4 out of 5 persons) answered that they felt more comfortable and fatigueless in garb A1 than in garb A and A2. It is concluded that local cooling in garb A1 of the upper torso could physiological reduce the thermal strain in participants wearing cooling vest.

• Journal of Microbiology
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• v.44 no.5
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• pp.492-501
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• 2006

In this study, we attempted to characterize the physiological response to oxidative stress by heat shock in Saccharomyces cerevisiae KNU5377 (KNU5377) that ferments at a temperature of $40^{\circ}C$. The KNU5377 strain evidenced a very similar growth rate at $40^{\circ}C$ as was recorded under normal conditions. Unlike the laboratory strains of S. cerevisiae, the cell viability of KNU5377 was affected slightly under 2 hours of heat stress conditions at $43^{\circ}C$. KNU5377 evidenced a time-dependent increase in hydroperoxide levels, carbonyl contents, and malondialdehyde (MDA), which increased in the expression of a variety of cell rescue proteins containing Hsp104p, Ssap, Hsp30p, Sod1p, catalase, glutathione reductase, G6PDH, thioredoxin, thioredoxin peroxidase (Tsa1p), Adhp, Aldp, trehalose and glycogen at high temperature. Pma1/2p, Hsp90p and $H^+$-ATPase expression levels were reduced as the result of exposure to heat shock. With regard to cellular fatty acid composition, levels of unsaturated fatty acids (USFAs) were increased significantly at high temperatures ($43^{\circ}C$), and this was particularly true of oleic acid (C18:1). The results of this study indicated that oxidative stress as the result of heat shock may induce a more profound stimulation of trehalose, antioxidant enzymes, and heat shock proteins, as well as an increase in the USFAs ratios. This might contribute to cellular protective functions for the maintenance of cellular homeostasis, and may also contribute to membrane fluidity.

• Steel and Composite Structures
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• v.45 no.3
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• pp.389-408
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• 2022

Residual capacity is defined as the load carrying capacity of an RC column after undergoing severe damage. Evaluation of residual capacity of RC columns is necessary to avoid damage initiation in RC structures. The central aspect of the current research is to propose an empirical formula to estimate the residual capacity of RC columns after undergoing severe damage. This formula facilitates decision making of whether a replacement or a repair of the damaged column is adequate for further use. Available literature mainly focused on the simulation of explosion loads by using simplified pressure time histories to develop residual capacity of RC columns and rarely simulated the actual explosive. Therefore, there is a gap in the literature concerning general relation between blast damage of columns with different explosive loading conditions for a reliable and quick evaluation of column behavior subjected to blast loading. In this paper, the Arbitrary Lagrangian Eulerian (ALE) technique is implemented to simulate high fidelity blast pressure propagations. LS-DYNA software is utilized to solve the finite element (FE) model. The FE model is validated against the practical blast tests, and outcomes are in good agreement with test results. Multivariate linear regression (MLR) method is utilized to derive an analytical formula. The analytical formula predicts the residual capacity of RC columns as functions of structural element parameters. Based on intensive numerical simulation data, it is found that column depth, longitudinal reinforcement ratio, concrete strength and column width have significant effects on the residual axial load carrying capacity of reinforced concrete column under blast loads. Increasing column depth and longitudinal reinforcement ratio that provides better confinement to concrete are very effective in the residual capacity of RC column subjected to blast loads. Data obtained with this study can broaden the knowledge of structural response to blast and improve FE models to simulate the blast performance of concrete structures.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.5
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• pp.605-611
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• 2015

A synthetic strain of ducks (Anas platyrhynchos) was developed by introducing genes for long duration of fertility to be used as mother of mule ducklings and a seven-generation selection experiment was conducted to increase the number of fertile eggs after a single artificial insemination (AI) with pooled Muscovy semen. Reciprocal crossbreeding between Brown Tsaiya LRI-2 (with long duration of fertility) and Pekin L-201 (with white plumage mule ducklings) ducks produced the G0. Then G1 were intercrossed to produce G2 and so on for the following generations. Each female duck was inseminated 3 times, at 26, 29, and 32 weeks of age. The eggs were collected for 14 days from day 2 after AI. Individual data regarding the number of incubated eggs (Ie), the number of fertile eggs at candling at day 7 of incubation (F), the total number of dead embryos (M), the maximum duration of fertility (Dm) and the number of hatched mule ducklings (H) with plumage colour were recorded. The selection criterion was the breeding values of the best linear unbiased prediction animal model for F. The results show high percentage of exhibited heterosis in G2 for traits to improve (19.1% for F and 12.9% for H); F with a value of 5.92 (vs 3.74 in the Pekin L-201) was improved in the G2. Heritabilities were found to be low for Ie ($h^2=0.07{\pm}0.03$) and M ($h^2=0.07{\pm}0.01$), moderately low for Dm ($h^2=0.13{\pm}0.02$), of medium values for H ($h^2=0.20{\pm}0.03$) and F ($h^2=0.23{\pm}0.03$). High and favourable genetic correlations existed between F and Dm ($r_g=0.93$), between F and H ($r_g=0.97$) and between Dm and H ($r_g=0.90$). The selection experiment showed a positive trend for phenotypic values of F (6.38 fertile eggs in G10 of synthetic strain vs 5.59 eggs in G4, and 3.74 eggs in Pekin L-201), with correlated response for increasing H (5.73 ducklings in G10 vs 4.86 in G4, and 3.09 ducklings in Pekin L-201) and maximum duration of the fertile period without increasing the embryo mortality rate. The average predicted genetic response for F was 40% of genetic standard deviation per generation of selection. The mule ducklings' feather colour also was improved. It was concluded that this study provided results for a better understanding of the genetics of the duration of fertility traits in the common female duck bred for mule and that the selection of a synthetic strain was effective method of improvement.

• Journal of the Korea Concrete Institute
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• v.19 no.2
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• pp.241-250
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• 2007

In case of retrofitting a concrete structure subjected to blast load by using retrofit materials such as FRP (fiber-reinforced polymer), appropriate ductility as well as raising stiffness must be obtained. But the previous approximate and simplified models, which have been generally used in the design and analysis of structures subjected to blast load, cannot accurately consider effects on retrofit materials. Problems on the accuracy and reliability of analysis results have also been pointed out. In addition, as the response of concrete and reinforcement on dynamic load is different from that on static load, it is not appropriate to use material properties defined in the previous static or quasi-static conditions to in calculating the response on the blast load. In this study, therefore, an accurate HFPB (high fidelity physics based) finite element analysis technique, which includes material models considering strength increase, and strain rate effect on blast load with very fast loading velocity, has been suggested using LS-DYNA, an explicit analysis program. Through the suggested analysis technique, the behavior on the blast load of retrofitted concrete walls using CFRP (carbon fiber-reinforced polymer) and GFRP (glass fiber-reinforced polymer) have been analyzed, and the retrofit capacity analysis has also been carried out by comparing with the analysis results of a wall without retrofit. As a result of the analysis, the retrofit capacity showing an approximate $26{\sim}28%$ reduction of maximum deflection, according to the retrofit, was confirmed, and it is judged ate suggested analysis technique can be effectively applicable in evaluating effectiveness of retrofit materials and techniques.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.2
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• pp.33-40
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• 2020

Various studies have been conducted on the structural health monitoring using optical fiber. Optical fibers can be used to measure multiple and distributed strain. Among the optical fiber sensors, FBG sensor has advantages of dynamic response measurement and high precision, but the number of measurement points is limited. Distributed fiber sensors, represented by distributed Brillouin sensors, usually have more than 1000 measurement points, but the low sampling rate makes dynamic measurements impossible. In this study, a hybrid nerve sensor system using only the advantages of the FBG sensor and the distributed Brillouin sensor has been proposed. Laboratory experiments were performed to verify the proposed system, and the accuracy and reproducibility were verified by comparing with commercial sensors. Applying the proposed system, dynamic response ambient measurements are used to evaluate the global state of the structure. When an abnormal condition is detected, the local condition of the structure is evaluated by static response measurement using the distributed measurement system. The proposed system can be used for efficient structural health monitoring.