• Title/Summary/Keyword: High NA objective

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Multi-sensor monitoring for temperature stress evaluation of broccoli (Brassica oleracea var. italica) (브로콜리(Brassica oleracea var. italica)의 온도 스트레스 평가를 위한 다중 센서 모니터링)

  • Cha, Seung-Ju;Park, Hyun Jun;Lee, Joo-Kyung;Kwon, Seon-Ju;Jee, Hyo-Kyung;Baek, Hyun;Kim, Han-Na;Park, Jin Hee
    • Journal of Applied Biological Chemistry
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    • v.63 no.4
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    • pp.347-355
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    • 2020
  • Several sensors have been developed for soil and plants to assess plant stress due to climate change. Therefore, the objective of the study is to nondestructively evaluate temperature stress on plant by monitoring climatic and soil conditions and plant responses using various sensors. Plant responses were monitored by electrical conductivity in plant stem and sap flow rate. Electrical conductivity in plant stem reflects the physiological activity of plants including water and ion transport. Fully grown Brassica oleracea var. italica was exposed to 20/15 ℃ (day/night) with 16 h photoperiods as a control, low temperature 15/10 ℃, and high temperature 35/30 ℃ while climatic, soil, and plant conditions were monitored. Electrical conductivity in plant stem and sap flow rate increased during the day and decreased at night. Under low temperature stress, electrical conductivity in plant stem of Brassica oleracea var. italica was lower than control while under high temperature stress, it was higher than control indicating that water and ion transport was affected. However, chlorophyll a and b increased in leaves subjected to low temperature stress and there was no significant difference between high temperature stressed leaves and control. Free proline contents in the leaves did not increase under low temperature stress, but increased under high temperature stress. Proline synthesis in plant is a defense mechanism under environmental stress. Therefore, Brassica oleracea var. Italica appears to be more susceptible to high temperature stress than low temperature.

Study for the Geochemical Reaction of Feldspar with Supercritical $CO_2$ in the Brine Aquifer for $CO_2$ Sequestration (이산화탄소의 지중저장 대염수층에서 과임계이산화탄소에 의한 장석의 지화학적 변화 규명)

  • Choi, Won-Woo;Kang, Hyun-Min;Kim, Jae-Jung;Lee, Ji-Young;Lee, Min-Hee
    • Economic and Environmental Geology
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    • v.42 no.5
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    • pp.403-412
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    • 2009
  • The objective of this study is to investigate the geochemical change of feldspar minerals by supercritical $CO_2$, which exists at $CO_2$ sequestration sites. High pressurized cell system (100 bar and $50^{\circ}C$) was designed to create supercritical $CO_2$ in the cell and the surface change and the dissolution of plagioclase and orthoclase were observed when the mineral surface reacted with supercritical $CO_2$ and water (or without water) for 30 days. The polished slab surface of feldspar was contacted with supercritical $CO_2$ and an artificial brine water (pH 8) in the experiments. The experiments for the reaction of feldspar with only supercritical $CO_2$ (without brine water) were also conducted. Results from the first experiment showed that the average roughness value of the plagioclase surface was 0.118 nm before the reaction, but it considerably increased to 2.493 nm after 30 days. For the orthoclase, the average roughness increased from 0.246 nm to 1.916 nm, suggesting that the dissolution of feldspar occurs in active when the feldspars contact with supercritical $CO_2$ and brine water at $CO_2$ sequestration site. The dissolution of $Ca^{2+}$ and $Na^+$ from the plagioclase occurred and a certain part of them precipitated inside of the high pressurized cell as the form of amorphous silicate mineral. For the orthoclase, $Al^{3+}$, $K^+$, and $Si^{+4}$ were dissolved in order and the kaolinite was precipitated. In the experiments without water, the change of the average roughness value and the dissolution of feldspar scarcely occurred, suggesting that the geochemical reaction of feldspars contacted with supercritical $CO_2$ at the environment without the brine water is not active.

Effect of Cation and Ionic Strength on Dispersion and Coagulation of Hwangto and Clay Minerals (양이온의 종류와 농도에 따른 황토와 점토광물의 분산과 응집)

  • Park, Bo-Kyeong;Kim, Kyung-Min;Kim, Young-In;Yum, Seo-Yun;Lee, Jeong-Woo;Hyung, Seuug-Woo;Hwang, Jun-Ho;Kim, Yu-Mi;Kong, Mi-Hye;Kim, Cheong-Bin;Roh, Yul
    • Journal of the Mineralogical Society of Korea
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    • v.22 no.3
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    • pp.249-259
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    • 2009
  • The objective of this research was to find out the physical properties, such as dispersion and coagulation, of soil minerals depending on the types and concentrations of the cations in aqueous solution. Hwangto samples were obtained from 90 to 130 cm from surface at Jangdong-ri, Donggang-Myon, Naju, Chonnam Province. The clay fraction (< $2\;{\mu}m$) was separated by sedimentation method from the bulk soils. Both Hwangto and clay fractions, and the same samples after removal of amorphous and crystalline iron oxides were used in this experiment. The effect of 4 cations ($Na^+$, $K^+$, $Mg^{2+}$, $Ca^{2+}$) and their concentrations on settling speed and basal spacing of the minerals were observed to examine the physical properties of the soil and clay minerals. Hwangto mainly consisted of quartz, and the clay fractions consisted of kaolinite, illite, and vermiculite. The bulk soils contained 16.3 mg/kg of amorphous iron oxides and 436 mg/kg of crystalline iron oxides. Clay fractions were dispersed better than bulk soils due to their smaller particle size than that of the bulk samples in the aqueous solution. The bulk and clay samples were dispersed better when iron oxides were removed because of coating of minerals by the iron oxides. Clay minerals were settled faster as the charge and the concentration of cations added increased. The d-spacing of kaolinite and illite did not change when 4 types of cations were added. The d-spacing of vermiculite showed $14.04\;{\AA}$ when divalent cations were added while that of vermiculite showed $13.9\;{\AA}$ when monovalent cations were added. It may be attributed to the hydration radii of cations. This study indicated that both coating of iron oxides on minerals and types and concentrations of cations affect dispersion of minerals in solution and d-spacing of expanding clay minerals such as vermiculite.

Factors Affecting the Property of $CaCO_3$Precipitated from $CaCl_2-Na_2CO_3-H_2O$ System ($CaCl_2-Na_2CO_3-H_2O$ 반응계에서 침강성탄산칼슘의 성상에 영향을 주는 인자에 관하여)

  • Song, Young-Jun;Park, Charn-Hoon;Cho, Dong-Sung
    • Resources Recycling
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    • v.5 no.4
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    • pp.32-41
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    • 1996
  • The objective of this study was to investigate the facton affecting the property of CaCO, farmed from CaClz-Na,CO,-HiOsystem. The effcct of the concentmtlon of reaclants, impurity, the pH of reaction, the addition of sccd crystal, and injectingvelocity af reaclant solution an thc yield oI CaCO; polymorphs. parlide size and whiteness of CaCO, were investigated. Thcmqor resulls are ;o fallows; I The optimum concentratinn of reildilnts for forming vaterlte and aragonite is the range of 0.1-1.0 mol/l, when the yicld of vittcrite and araga~nles howed 7542% and XU-90%. respedively. 2. Among thc composition of impunticscontained h limestone, Fe' decrease the wh~tcness nf CaCO;. md Mg" increase the yield of aragonite. 3. The pHrange of vaterite and aragonite are formed with high yield is 8-11, and Calcite is famed in pH 6-8 with big particle size of 1over and in pH 11-13 with small particle size of I under. 4. The yicld of calcite and aragonite was increased by addingthc seed cryst.al nf itself.d cryst.al nf itself.

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Evaluation for Impacts of Nitrogen Source to Groundwater Quality in Livestock Farming Area

  • Lee, Gyeong-Mi;Park, Sunhwa;Kim, Ki-In;Jeon, Sang-Ho;Song, Dahee;Kim, Deok-hyun;Kim, Tae-Seung;Yun, Seong-Taek;Chung, Hyen Mi;Kim, Hyun-Koo
    • Korean Journal of Soil Science and Fertilizer
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    • v.50 no.5
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    • pp.345-356
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    • 2017
  • We investigated 52 livestock farming complexes in Gyeong-Gi and Incheon provinces based on low, medium, and high livestock density and groundwater quality. The objective of this study was to evaluate a relationship between nitrate N concentration in groundwater and animal factors, such as livestock density and animal species. 2,200 groundwater samples for 3 years from 2012 to 2014 at Gyeong-Gi and Incheon provinces were collected and analyzed for pH, EC, DO, ORP, temperature, major anions and cations, such as $NO_3-N$, ${HCO_3}^-$, ${PO_4}^-$, ${SO_4}^{2-}$, $Cl^-$, $NH_4-N$, $K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$, T-N, and TOC. Average concentration of total N for generated load density was $23,973g\;day^{-1}\;km^{-2}$ for cattle, $51,551g\;day^{-1}\;km^{-2}$ for pig, and $52,100g\;day^{-1}\;km^{-2}$ for poultry. For animal feeding species, average ratio for generated load over discharge load was 16.1% for cattle, 7.8% for pig, and 7.1% for poultry. Therefore, cattle feeding region is highly vulnerable for water pollution compared to pig and poultry feeding areas. The concentrations of chloride, nitrate, and total N in the groundwater samples were higher at high animal farming regions than other regions. The average concentration of nitrate, and chloride in groundwater samples was $5.0mg\;L^{-1}$, $16.6mg\;L^{-1}$ for low livestock density, $6.9mg\;L^{-1}$, $17.7mg\;L^{-1}$ for medium livestock density and $7.6mg\;L^{-1}$, $22.7mg\;L^{-1}$ for high livestock density and total nitrogen (T-N) was $7.7mg\;L^{-1}$ for low livestock density, $9.4mg\;L^{-1}$ for medium livestock density, $10.7mg\;L^{-1}$ for high livestock density. In conclusion, based on this research, for managing groundwater quality near livestock farming regions, $Ca-(Cl+NO_3)$ group from the Piper diagram is more efficient than using 19 factors for water quality standard.

The Estimation of Subjective Evaluations for Impact Sound and Analysis of the Effects for Parts of a Car (자동차 임팩트 사운드에 대한 주관적 평가 및 차량 개발에 응용)

  • Na, Eun-Woo;Park, Sang-Won;Kim, Ho-Wok;Lee, Sang-Kwon;Lee, Kyung-Hoi;Shin, Young-Gon;Bae, Byung-Kook
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2009.10a
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    • pp.137-142
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    • 2009
  • Impact noise is induced in a car when it is driven on a harsh road or over some bumps. This noise occurs with the very high level of sound, which affects passengers in some way or other. Although it is impossible to clearly remove such noise. It is necessary to research an improvement in sound quality for impact noise. A new sound metric for impact sound is presented in the previous work. This metric is verified by comparison between mean subjective ratings and several sound metrics. In this paper, more objective attributes are considered, which are the attributes expressing the level and modulation of sound. Three sound metrics are employed to get impact sound indexes for each course by the method of multiple linear regressions. The indexes are verified by considering the correlation between the estimated values from the multiple linear regressions and the mean subjective ratings by evaluators. Also, the subjective ratings on the indexes are estimated for the case in which some parts of suspension system are changed. The estimated ratings represent more reasonable or acceptable ratings. Thus, such indexes can be used for modification of the parts of suspension system under considering a good sound quality.

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Effect of Do-In (Prunus persica L. BATSCH) Water Extract (PPE) on Concentration of Extracellular Acetylcholine in the Rat Hippocampus

  • Gong Dae-Jong;Kim Geun-Woo;Koo Byung-Soo
    • The Journal of Korean Medicine
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    • v.25 no.4
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    • pp.1-7
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    • 2004
  • Objective : This study was designed to examine the effects of orally administered Prunus persica water extract (PPE), which is used as herbal medicine, for treatment of Yu Xue (stasis of blood) and tacrine on the basal concentration of extracellular acetylcholine in the hippocampus of rats. Methods: To investigate the effects of PPE and tacrine on concentration of extracellular acetylcholine in the hippocampus of rats, the microdialysis technique, under the same experimental conditions, was used. And we used male Wistar rats which were 7 weeks of age and 210-290 g. PPE was extracted with boiling water, and the rats were anesthetized with pentobarbital Na. Their skulls were exposed and a hole was drilled for implantation of a microdialysis probe. In order to increase the recovery of acetylcholine, a probe with a long membrane was used. One day after surgery, the microdialysis probe was perfused with Ringer's solution at a flow rate of 1.5 l/min. The acetylcholine concentration in dialysis samples was measured by high-performance liquid chromatography (HPLC) with electrochemical detection. AChE activity was measured using the radiometric method, as described by Sherman. Results : The comparative effects of PPE and tacrine on hippocampal extracellular acetylcholine concentration was that these cholinesterase inhibitors produced dose-dependent increases in the extracellular acetylcholine concentration. And the effect of PPE and tacrine on rat brain AChE activity was that PPE produced maximal inhibition at 1 h after administration, when AChE activity was 44% of the intact level. AChE activity gradually recovered thereafter, and reached 78% of the intact level at 12 h after administration. Conclusion : In this study, PPE has a potent activity and a long-lasting effect on the central cholinergic system, in terms of the basal concentration of extracellular acetylcholine in the hippocampus and the AChE activity in the brain of rats. And oral administration of PPE increased dose-dependently the basal concentration of extracellular acetylcholine in the hippocampus of rats. PPE may be one of the more useful cholinesterase inhibitors for the treatment of Alzheimer's disease.

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Influence of Initial Seedling Size and Root Pruning Intensity on Growth of Transplanting Seedling of Quercus acutissma

  • Na, Sung-Joon;Lee, Do-Hyung;Kim, In-Sik
    • Korean Journal of Plant Resources
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    • v.26 no.6
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    • pp.709-717
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    • 2013
  • The objective of this study was to examine the effect of root pruning intensity in combination with different initial seedling size on the growth of transplanting seedlings of Quercus acutissima. One-year-old seedlings were divided into three groups depending on their height, i.e. small (< 15 cm), medium (25-35 cm) and large size (35 cm <). Root of seedlings was pruned by three intensity such as, leaving 5 cm (severe), 10 cm (medium) and 15 cm (light) of taproot from the root-collar. After one year, we investigated survival rate, height and root-collar diameter (RCD) increment and final shoot dry weight. Also we measured characteristics of newly developed lateral roots such as number, total length, dry weight and diameter. Severe root pruning showed the lowest survival rate in all seedling size. Height increment, RCD increment and shoot dry weight were decreased with increasing intensity of root pruning. Seedlings of medium and light root pruning showed similar above-ground growth and dry weight of lateral roots. More large seedlings showed good survival rate, height increment and final shoot dry mass in all root pruning intensity. Therefore, one-year-old seedlings of Q. acutissima should be pruned taproot by 10 cm and transplanted to obtain excellent performance and increase the efficiency of transplanting work. Based on the findings of this study, it is important that applying to different root pruning intensity depending on initial seedling size for producing 2-year-old seedlings with excellent growth and high quality.

Effect of Marital Conflict on Adolescent Anxiety: The Mediating Role of Parentification (부부갈등이 청소년의 불안에 미치는 영향: 청소년 부모화의 매개적 역할)

  • Yang, Han-Na;Choi, Mi-Kyung
    • Korean Journal of Child Studies
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    • v.38 no.3
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    • pp.49-63
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    • 2017
  • Objective: The main purpose of this study was to examine the relationship between marital conflict, parentification, and adolescent anxiety. Methods: The participants comprised 301 high school students from the Seoul province. They were asked to complete questionnaires that consisted of the State-Trait Anxiety Inventory, the Children's Perception of Interparental Conflict Scale, and the Filial Responsibility Scale-Youth. The data were analyzed by frequency analysis, Cronbach's ${\alpha}$, descriptive statistical analysis, Pearson's correlation coefficients, multiple regression analysis, and hierarchical multiple regression analysis. To analyze the collected data, SPSS 18.0 was used. Results: First, the content and resolution of marital conflict had an effect on both adolescent state anxiety and trait anxiety. Second, unfairness parentification had an effect on both adolescent state anxiety and trait anxiety. Third, the frequency of marital conflict had an effect on emotional parentification. Further, the intensity, content, and resolution of marital conflict had effect on unfairness parentification. Fourth, unfairness parentification played a partial mediating role in the relationship between the content of marital conflict and adolescent anxiety (state anxiety/trait anxiety). In addition, unfairness parentification played a complete mediating role in the relationship between the resolution of marital conflict and adolescent trait anxiety. Conclusion: These results indicate that parentification plays an important role in marital conflict and adolescent anxiety. In addition, this study also shows that marital conflict has an important effect on adolescent anxiety directly. Therefore, this study will be useful for developing both parental education and counseling programs for adolescents.

Antioxidant and Anti-inflammatory Effect of Angelica Tenuissima in IFN-${\gamma}$/LPS-stimulated Peritoneal Macrophage (IFN-${\gamma}$와 LPS로 자극된 쥐의 복강 대식세포에서 고본(藁本) 메탄올 추출물의 항염증 효과)

  • Lee, Se-Yeoun;Park, Ho-Jun;Cha, Dong-Seok;Shin, Tae-Yong;Na, Ho-Jeong;Moon, Woo-Sung;Kang, Yang-Gyu;Jeon, Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.6
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    • pp.1549-1556
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    • 2008
  • The objective of this study were to evaluate the antioxidant activity and the anti-inflammatory effects of Angelica tenuissima (AT) which has been used widely as a traditional medicine. The antioxidant activities of AT was tested by DPPH radical scavenging, superoxide anion scavenging and nitric oxide scavenging. AT showed strong antioxidant activity in all experiment. In macrophages nitric oxide (NO) is released as an inflammatory mediator and has been proposed to be an important modulator of many pathophysiological conditions and high concentratin of NO is produced by inducible nitric oxide synthase (iNOS). In this study we have examined the inhibition effects of NO by 85% methanol extracts of AT in mouse (C57BL/6) peritoneal macrophage. AT (100, 1000 ${\mu}g/m{\ell}$) suppressed nitric oxide production and iNOS expression without any notable cytotoxicity and it also inhibited the expression of inflammatoryenzymes like cyclooxygenase-2 (COX-2). These data suggest that 85% methanol extracts of AT may possibly be used as antioxidant and anti-inflammatory agent.