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Biochemical properties of a purified protein in cystic quid of Taenia solium metacestodes (유조낭고충 낭액에서 친화성 크로마토그래피로 분리한 항원 단백질의 생화학적 성상)

  • Cho, Seung-Yull;Kim, Suk-Il;Kang, Shin-Yong;Kong, Yoon
    • Parasites, Hosts and Diseases
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    • v.26 no.2
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    • pp.87-94
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    • 1988
  • By affinity chromatography using a monoclonal antibody as ligand, Kim et at. (1986) purified a protein fraction in cystic fluid of Taenia solium metacestodes (CF) In this study, the biochemical properties of the purified protein were characterized. Discontinuous-polyacrylamide gel electrophoresis (disc-PAGE) of the protein at 4.5∼10% separating gel concentration showed its molecular weight (MW) to be 150 kilodalton (kDa) in non·denatured state, while denaturing sodium dodecyl suifate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that it was composed of 3 different subunits with respective fnw of 15, 10 and 7 kDa. Subunit of 7 kDa was shown to be linked to other subunits by disulade bonds. Isoelectric point of the protein was pH 6.8. The protein was relatively heat-stable for immunologic analysis. These properties indicated that the protein, comprising about 70% of total content in CF, had similar biochemical characters with antigen B of Oriol et at.(1971) in hydatid cyst quid (HF).

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Circadian Disruptions of Heart rate Variability among Weekly Consecutive-12-hour 2 Shift Workers in the Automobile Factory in Korea (한 자동차공장의 1주연속 12시간주야맞교대근무 노동자들의 심박동수변이)

  • Sung, Ju-Hon;Yum, Myung-Gul;Kong, Jung-Ok;Lee, Hye-Un;Kim, In-A;Kim, Jung-Yeon;Son, Mi-A
    • Journal of Preventive Medicine and Public Health
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    • v.37 no.2
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    • pp.182-189
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    • 2004
  • Objectives : The objective of this study is to compare the circadian patterns of heart rate variability assessed by 24-hour ambulatory electrocardiographic (ECG) recordings during day shift and night shift among the workers in the 5 days-concecutive-12-hour shift in an automobile factory in Korea. Methods : The study population consisted 300 workers, who were randomly selected among the 8700 total workers in one car factory. To analyse circadian variation, the 24-hour ECG recordings (Marquette) were measured during day shift (08:00-20:00 h) and night shift (20:00-08:00 h). Analysis was performed for all time and frequency domain measures of HRV. 233 workers completed taking 24-hour ECG recordings. Results : This study shows that the 24 hourcircadian variation mainly follows work/sleep cycle rather than day/night cycle among shift workers. This study also shows that among the night shift, the circadian variation between work and sleep cycle decreased compared to the work/sleep cycle among day shift workers. All time and frequency domain parameters (except LF/HF ratio) show significantly different between work and sleep in the day shift and night shift. Conclusion : These changes in heart rate variability circadian rhythms reflect significant reductions in cardiac parasympathetic activity with the most marked reduction in normal vagal activity among the shift workers. Especially, it suggests the circadian rhytm has blunted among the night workers. The quantification of the circadian variation in HRV can be a surrogates of workers' potential health risk, as well as suggests possible mechanisms through which the shift works compromise workers' health.

Psychophysiological Responses Evoked by Fear and Disgust Emotion Using Audiovisual Film Clips in Children (공포와 혐오 정서에 대한 아동의 심리생리반응)

  • Jang, Eun-Hye;Woo, Tae-Je;Lee, Young-Chang;Sohn, Jin-Hun
    • Science of Emotion and Sensibility
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    • v.10 no.2
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    • pp.273-280
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    • 2007
  • The study is to examine the psychophysiological responses evoked by negative emotions(fear and disgust) in children. 47 children(11-13 years old, 23 boys) participated in the study. While the children were experiencing fear or disgust emotion induced by audio-visual film clips, ECG, EDA, PPG and SKT are measured. Emotion assessment scale was used to confirm that emotions elicited by the film clips were significantly noticeable, which was measured self-report. The results turned out to be 100% and 89.4% of appropriate for fear and disgust emotions, respectively. Emotional intensity the children had experienced was rated as 4.05, 4.07 on 1-5 scale based on effectiveness of measurement of fear and disgust emotion. ANS reponses by fear and disgust were significantly between the resting state and emotional state induced. The result obtained from the fear emotion showed significant increases in SCL, NSCR, HR, RSA, RESP and HF. There was a significant difference in SCL and NSCR between the two emotions.

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Effect of Ni Catalyst Thickness on Carbon Nanotube Growth Synthesized by Hot-filament PECVD (Ni 촉매층의 두께가 탄소나노튜브의 성장 형태에 미치는 영향)

  • Kim, Jung-Tae;Park, Yong-Seob;Kim, Hyung-Jin;Choi, Eun-Chang;Hong, Byung-You
    • Journal of the Korean Vacuum Society
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    • v.16 no.2
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    • pp.128-133
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    • 2007
  • In this study, we observed the shapes of CNTs formed with the thinckness of catalyst. Catalyst layer was grown by magnetron sputtering method and the thickness of Ni catalyst is the range from 20 to 80 nm. Also, the synthesis of CNT with Ni catalyst thickness was grown by hot-filament PECVD method. And, we investigated the composition of CNTs by using EDS measurement, also observed the shapes of CNTs by using HRTEM and FESEM measurements. In the result, through the TEM analysis, we observed the empty inside of CNTs and the multiwall CNTs, also confirmed the tip of CNT containing Ni. The composition of CNTs are consisted of an element of C, Ti, and Ni. As you shown the growth shapes of CNTs, the pretreatment of the catalyst before te growth of CNTs changed the particle size of the catalysts and grown the CNTs of the different shapes. Consequently, the best vertically alined and well-arranged CNTs exhibited from the substrate deposited at the catalyst thickness of 40 nm.

The Study on Mössbauer Spectroscopy of Zn1-xFexO (Zn1-xFexO의 뫼스바우어 분광학적 연구)

  • Kim, S.J.;Lee, S.R.;Park, C.S.;Kim, E.C.;Joh, Y.G.;Kim, D.H.
    • Journal of the Korean Magnetics Society
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    • v.18 no.2
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    • pp.75-78
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    • 2008
  • $AB_2X_4$(A, B=Transition Metal, X=O, S, Se) are cubic spinel normal ferrimagnets, in which M ions occupy the tetrahedral sites and Cr ions occupy the octahedral sites. Recently, they have been investigated for behaviour of B site ions and A-B interaction. Polycrystalline $[Co_{0.9}Zn_{0.1}]_A[Cr_{1.98}{^{57}Fe_{0.02}}]_BO_4$ compound was prepared by wet-chemical process. The ferrimagnetic transition was observed around 90K. $M\"{o}ssbauer$ absorption spectra at 4.2K show that the well-developed two sextets are superposed with small difference in hyperfine fields($H_{hf}$). The hyperfine fields of $CoCr_{1.98}{^{57}Fe_{0.02}}O_4$ and $Co_{0.9}Zn_{0.1}Cr_{1.98}{^{57}Fe_{0.02}}O_4$ were determined to be 488, 478 kOe and 486, 468 kOe, respectively. We notice that the one of the magnetic hyperfine field values changes with Zn ion substitution. These results suggest the incommensurate states and spin-reorientation temperature($T_S=18K$) changes with Zn ions substitution below spin-reorientation temperature($T_S=28K$) of $CoCr_{1.98}{^{57}Fe_{0.02}}O_4$

Effect of Chromium Methionine Supplementation on Obesity Index and Serum Lipids in Rats (Chromium Methionine첨가가 비만지수 및 지질 대사에 미치는 영향)

  • Kim, Hyun-Sook;Lee, Sun-Yeon;Kim, Chang-Hyeuk;Ohh, Sang-Jip;Shin, Jong-Seo;Sung, Kyung-Il
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.5
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    • pp.706-709
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    • 2003
  • Chromium (Cr) plays an important role in body composition. Several human and animal studies of Cr supplementation have reported increases in lean body mass, often with concurrent decreases in body fat. The purpose of the current study was to determine the effects of supplemental Cr as chromium methionine (CrMet) on obesity index, body fat and serum lipids in rats. Thirty-six male Sprague-Dawley rats were divided into four groups of nine rats. Rats received a AIN-76 semipetrified basal diet or high fat diet supplemented with 300 ppb CrCl$_3$and CrMet as well as no added dietary Cr (referred to as Control, HF, HFCr, and HFCrMet). After 6 weeks on the respective diets, animals were killed and analyzed for differences in serum lipid concentrations. Rats supplemented with CrMet had a slightly higher naso-anal length and final body weight was decreased. The lowest obesity index and body fat content were found in CrMet supplemented animals (p〈0,05). Supplemental CrMet decreased the total lipid, triglyceride and total cholesterol. The significant differences observed were total cholesterol concentration for CrMet supplementation. These indicate that supplemental Cr, as chromium methionine, resulted in a significant decrease of obesity index and body fat and influence serum lipid concentrations, possibly by decreasing the body fat.

Changes in Hydration and Watertightness of Cement Containing Two-Component Fluosilicate Salt Based Chemical Admixture (2성분 규불화염계 혼화제가 첨가된 시멘트의 수화반응 및 수밀성 변화)

  • Kim, Jae-On;Nam, Jae-Hyun;Kim, Do-Su;Khil, Bae-Su;Lee, Byoung-Ky
    • Journal of the Korean Ceramic Society
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    • v.41 no.10 s.269
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    • pp.749-755
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    • 2004
  • Fluosilicic acid ($H_2SiF_6$) is recovered as aqueous solution which absorbs $SiF_4$ produced from the manufacturing of industrial-graded $H_3PO_4$ or HF. Generally, fluosilicate salts prepared by the reaction between $H_2SiF_6$ and metal salts. Addition of fluosilicate salts to cement endows odd properties through unique chemical reaction with the fresh and hardened cement. In this study, two-component fluosilicate salt based chemical admixtures (MZ) of $4\%,\;6\%$, and $8\%$ concentration were prepared by the reaction of $H_2SiF_6$ ($25\pm2\%$) and metal salts. The effect of concentration of MZ at a constant adding ratio on the hydration and watertightness of cement were investigated respectively. In a cement containing MZ, metal fluorides such as $CaF_2$ and soluble silica by hydrolysis were newly formed during hydration. The total porosity of the hardened cement was lower in the presence of U because of packing role of metal fluoride and pozzolanic reaction of soluble $SiO_2$. Consequently, the watertightness of the hardened paste containing MZ was more improved than non-added (plain) due to an odd hydration between cement and MZ.

A Study on the Implementation of Terminal System for the Fishing Ship Using Digital Fishing Network (디지털 어업통신망을 위한 어선용 단말기 구현 방안 연구)

  • Kim Jeong-nyun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.8
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    • pp.1620-1625
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    • 2004
  • To advance fisheries, we set developmental directions of fishery information by grasping present situations and analyzing maritime & fisheries issues. We promote various policies through effective systematical information data bases, based on both control and utilization of oceanic resources. For these puposes, it is imperative that we set up fisheries communication networks. There are satellite assisted informational networks to assist fishing vessels with their marine based movements. However, there's no hope for poorly equipped fishermen to adopt this network because of extravagant network call charges. So we think that using existing SSB communication system is the best plan. We organize fishery communication network by HF SSB communication which doesn't have operational costs. We build wireless transmitting and receiving stations that are basic systems of informnation, and equip wireless data communication systems by the use of wireless communication network protocols in coastal stations. It is necessary that a fish boat has a terminal device for wireless data communication. In this research we can conclude that if we transmit the location of a fishing boat in-real time through GPS channels then we propose that some methods be formulated to able terminal devices on fishing boats to collect various types of information, such as meteorological and oceanic conditions.

The Effect of Uhwangchungsimwon on Heart Rate Variability of Healthy Subjects (우황청심원이 정상인의 심박변이도에 미치는 영향)

  • Choi, Chang-Min;Sun, Jong-Joo;Kim, Seok-Min;Jung, Jae-Han;Lee, Seung-Yeup;Choi, Won-Woo;Hong, Jin-Woo;Park, Seong-Uk;Jung, Woo-Sang;Moon, Sang-Kwan;Park, Jung-Mi;Ko, Chang-Nam;Cho, Ki-Ho;Kim, Young-Suk;Bae, Hyung-Sup
    • The Journal of Internal Korean Medicine
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    • v.28 no.4
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    • pp.717-726
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    • 2007
  • Objectives : This study was conducted to assess the effect of Uhwangchungsimwon on the autonomic nervous system. Methods : Eligible subjects were enrolled from healthy people in their 20s-30s. They were divided into two groups, the UC group (n=22) and the control group (n=20). We monitored ECG from 14:00 to 18:00 in both groups. In the UC group, subjects were administered UC at 15:00. HRV analysis was conducted at 14:00, 16:00, and 17:00 for 512 seconds. Results : The degree of LF/HF ratio change was significantly higher in the UC group at 1 hour after administration. Conclusion : We suggest Uhwangchungsimwon may be useful for stabilization of the autonomic nervous system by activating parasympathetic nerve in healthy people.

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Deficiency-excess and Cold-heat Pattern Identification and Analysis of the Characteristics of Asthma Patients (천식(喘息) 환자의 허실한열변증(虛實寒熱辨證)과 특성 비교 분석)

  • Bhang, Yeon-hee;Kim, Jae-hyo;Do, Ha-yoon;Kim, Mi-a;Kim, Kwan-il;Lee, Beom-joon;Jung, Hee-jae
    • The Journal of Internal Korean Medicine
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    • v.38 no.6
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    • pp.955-970
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    • 2017
  • Objectives: The aim of this observational study was to identify and analyze the patterns to compare the characteristics of asthma patients. Methods: The subjects were 40 asthma patients who had satisfied the inclusion and exclusion criteria. They were divided into deficiency syndrome and excess syndrome groups, and cold syndrome and heat syndrome groups. Their quality of life was measured by the quality of life questionnaire for adult Korean asthmatics (QLQAKA) and VAS. Heart rate variability (HRV) was measured, and the degree of obesity was evaluated by body mass index (BMI). Hematological, biochemical, and immunoglobulin (Ig) E laboratory tests were included. Results: Based on pattern identification, the 40 asthma patients could be divided into two categories of groups: 1) the deficiency syndrome (N=18) and the excess syndrome (N=22) groups: 2) the cold syndrome (N=35) and the heat syndrome (N=5) groups. The mean value of HF differed significantly between the deficiency and excess syndrome groups. The mean value of IgE in blood tests of asthmatics was greater than four times the reference value. For BMI, the subjects were classified into three groups: normal weight (N=12), overweight (N=12), and obese (N=16). Conclusions: Development of a more accurate asthma-specific pattern identification tool could play a crucial role in asthma control. In addition, good control of asthma can improve the quality of life. Obesity is one of the factors associated with asthma exacerbation.