• 제목/요약/키워드: Hexane extracts

검색결과 838건 처리시간 0.028초

추출조건에 따른 인삼농축액 중의 잔류용매 및 무기물 함량 (Residual Solvents and Mineral Contents in Ginseng Extracts with Different Extracting Conditions)

  • 이선화;김우성;김용무;홍영표;안영순;박홍재;김옥희
    • 한국환경과학회지
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    • 제16권1호
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    • pp.39-44
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    • 2007
  • This study was conducted to investigate the contents of residual solvents and mineral components(11 kinds) in ginseng extracts with different extracting conditions(5 types) and commercial ginseng extract products(domestic, imported). Fine root was extracted with solution having various ethanol concentration after hexane treatment. Among 5 type extracts, residual solvent(hexane) was detected ginseng extracts treated ethanol mixed with hexane. But extracts that dried after soaked in hexane wasn't detected hexane. Mineral components(Al, Mn, Fe, Cu and Zn) were detected in fine root and 5 types of extracts. The contents of mineral components between fine root and extracts with various extracting conditions were similar, however, extracts that dried after soaked in hexane showed the lower amount in Al, Fe, Pb than the others. In comparison with commercial ginseng ex-tract products(domestic, imported), the distribution pattern of mineral was similar but the contents were a little different.

n-Hexane에 의한 삼백초 추출물의 항균 및 항바이러스 활성 (Antimicrobial and antiviral activity of Saururus chinensis extract by n-Hexane)

  • 이주현;최영호;박윤진;장효완;김병수
    • 한국동물위생학회지
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    • 제36권2호
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    • pp.87-93
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    • 2013
  • This study was conducted to investigate the antimicrobial, antivirus properties of Saururus chinensis extracts. The n-hexane extracts from Saururus chinensis showed the active antimicrobial activity against gram-positive bacteria. Minimum inhibitory concentration (MIC) of Saururus chiensis n-hexane extracts was 1.25 mg/ml against B. subtilis and 2.5 mg/ml against S. aureus. The cytotoxicity effects on MDBK (Madin-Darby bovine kidney) cell were observed at the various n-hexane extract concentrations. In $TCID_{50}$ assay, 0.6 mg/ml of n-hexane extracts decreased BVD (bovine viral diarrhea) virus by 1.4 log, whereas other extracts did not show antiviral activity. In this study, The results suggested that n-hexane extracts and fractions of Saururus chinensis can be a candidate materal of feed additive to chemical antibiotics and antivirus substances.

고들빼기 잎추출물이 흰쥐의 사염화탄소에 의한 간손상에 미치는 영향 (Effects of Godulbaegi Leaf Extracts on $CCl_4$-Induced Hepatotoxicity in Rats)

  • 배송자;김남홍;하배진;정복미;노승배
    • 한국식품영양과학회지
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    • 제26권1호
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    • pp.137-143
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    • 1997
  • 본 연구는 고들빼기를 분획별로 나누어 수층, butanol 층, hexane층, ethyl acetate층 분획 물들을 1주일간 흰 쥐의 복강내 주사 후 마지막날 사염화탄소를 복강내 1회 투여하여 간손상을 유발시킨 쥐의 간중량, 과산화지질, 항산화효소활성 및 간조직을 관찰하였다 체중에 대한 간중량 백분율은 간손상군중에서 n-hexane 분획물 투여군이 가장 낮게 나타났으며, 혈청 중 GPT활성 역시 n-hexane 분획물 투여군이 거의 정상군과 비슷한 수준으로 떨어 졌다. 간 중 MDA함량은 고들빼기 분획물 투여군들이 모두 낮은 경향이었으며 그 중에서도 n-butanol 분획물 투여군과 n-hexane 분획물 투여군이 대조군에 비해 유의적으로 낮았다 간 중 GST활성은 정상군에 비해 간손상군이 떨어졌으며 간손상군에서는 대조군에 비해 ethyl acetate 분획물 투여군이 유의적으로 높게 나타났다. 간조직 관찰에서는 정상군에 비 해 간손상군의 조직에 염증 및 괴사가 뚜렷이 나타났고 고들빼기 n-hexane 분획물 투여 후 간독성 유발 쥐의 간조직에서는 염증 및 괴사가 확실히 감소한 경향을 볼 수 있었다.

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Activities of Natural Plant Extracts against HIV-1

  • Eum, Jin-Seong;Park, Young-Doo;Hong, Seong-Karp
    • Journal of information and communication convergence engineering
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    • 제7권4호
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    • pp.576-579
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    • 2009
  • Anti-HIV-1 activities for the extracts (buthanol, hexane, chloroform, and water) of medicinal plants widely used in the folk medicine were evaluated for screening of anti-AIDS agents. The activities of the extracts to inhibit HIV-1 replication were also analyzed. The 50% effective concentration (EC50) of inhibition activity of the p24 production for chloroform extract of Saphora flavescens, chloroform extract of Herba ephedrae, and hexane extract of Pachyma hoelen Rumph showed 5.8, 29.9, and 37.3 2g/ml, respectively, as good activities. Hexane extract of Sophora flavescens, buthanol extract of Tulipa edulis, hexane extracts of Tulipa edulis, Herba ephedra, and Pachyma hoelen Rumph in the 50% cytotoxic concentration ($CC_{50}$) in inhibition activities of recombinant HIV-1 RT showed 12.9, 19.5, 11.6, 12.0, and 36.8 % at concentration of 200 ${\mu}g$/ml, respectively, as good activities. From these results, chloroform extract of Saphora flavescens, chloroform extract of Herba ephedrae, and hexane extract of Pachyma hoelen Rumph were very effective against HIV-1 among all extracts tested. Therefore, we expect these plants will be a useful for anti- HIV-1 therapeutics in future.

Hepatoprotective Effect of Stamen Extracts of Mesua ferrea L. against Oxidative Stress induced by $CCl_4$ in Liver Slice Culture Model

  • Rajopadhye, Anagha A.;Upadhye, Anuradha S.
    • Natural Product Sciences
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    • 제18권2호
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    • pp.76-82
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    • 2012
  • Stamens of Mesua ferrea L. are a well-known herbal drug used in Indian System of Traditional Medicine to treat various diseases. The claimed activity of this plant part is necessitated to investigate antioxidant and hepatoprotective activity. Authenticated plant sample was extracted with hexane, ethanol (EtOH) and water (aq.) using ASE 100 accelerated solvent extractor. Antioxidant activity was evaluated by means of different in vitro assays. Hepatoprotective effect was investigated on carbon tetrachloride induced oxidative stress in liver slice culture model. Cytotoxic marker lactate dehydrogenase (LDH) released in culture medium and the activity of lipid peroxidation along with antioxidant enzymes (AOEs) namely superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were estimated. Hexane and EtOH extracts were significantly inhibited DPPH, NO, SOD and $ABTS^+$ radical in dose dependent manner. The trade of phenol content was: aq. extract < hexane extract < EtOH extract. A significant correlation was shown by total phenol content and free radical scavenging activity of extracts. The culture system treated with hexane extract, EtOH extract or ascorbic acid exhibited significant depletion in LDH, lipid peroxidation, antioxidative enzymes SOD, CAT and GR. Hexane extract and EtOH extracts of stamen of M. ferrea protected liver slice culture cells by alleviating oxidative stress induced damage to liver cells.

Antioxidant Properties of Ginseng (P. ginseng C.A. Meyer) Extracts by Organic Solvent Fractionation

  • Kim, Ji-Sang;Moon, Gap-Soon;Kim, Hyun-Oh;Lee, Young-Soon
    • Preventive Nutrition and Food Science
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    • 제12권4호
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    • pp.267-272
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    • 2007
  • The objective of this study was to investigate antioxidant activities of Panax ginseng extracted with various solvents including n-hexane, chloroform, EtOAC, n-butanol and water. Among the various ginseng extracts, ethyl acetate (EtOAC) extracts showed the most powerful scavenging activities against DPPH radicals. Among the other solvent extracts, the butanol extract seemed relatively more effective in scavenging activity, followed by chloroform, water and hexane extracts. Moreover, the highest reducing power and ferrous ion chelating activity were found in the EtOAC extract followed by other extracts of ginseng. EtOAC extracts, which exhibited the best antioxidant activities of all solvent extracts of ginseng, possessed higher concentrations of total phenolics (777.61 mg/100 g) than other extracts. These results suggest that EtOAC extracts of ginseng (P. ginseng C.A. Meyer) have the most effective antioxidant capacity compared to n-hexane, chloroform, n-butanol and water tested in this study, and has important applications for the pharmaceutical and food industries.

간암 및 자궁암 세포주 증식에 미치는 오매 추출물의 영향 (The Effect of Prunus Mume Extracts on the Growth of HepG2 and HeLa Cell Lines)

  • 배지현;정승은
    • Journal of Nutrition and Health
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    • 제35권4호
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    • pp.439-445
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    • 2002
  • 본 연구에서는 예로부터 민간과 한방에서 널리 이용되어 온 오매를 이용하여 오매 추출물이 간암 및 자궁암 세포주 증식에 미치는 효과를 알아보았다. 오매를 hexane. chloroform, ethylacetate, methanol 및 열수로 순차 추출하여 분획물을 농도별로 조제하였으며, MTT assay, cytotoxicity test 및 현미경 관찰 등을 통하여 암세포의 성장 억제 효과를 알아보았다. Hep G2의 경우 오매의 ethylacetate추출물이 가장 큰 성장 저해 효과를 나타내었으며 MTT assay결과 250 $\mu\textrm{g}$/ml 농도 이상에서부터 유의적인 증식 억제 효과를 보였다. 또한 cytotoxicity test결과 오매의 ethylacetate 추출물 1000$\mu\textrm{g}$/ml농도에서 72시간 배양시 75%의 세포 파괴율을 나타내었으며 현미경 관찰 결과 세포의 모양이 변화되고 죽은 세포가 배양액위에 떠있음을 관찰할 수 있었다. HeLa cell에서는 오매의 hexane 추출물이 가장 큰 저해 효과를 나타내었으며 MTT assay 결과 250 $\mu\textrm{g}$/ml 농도 이상에서부터 유의적인 증식 억제 효과를 보였다 또한 cytotoxicity test 결과 오매의 hexane추출물 1000 $\mu\textrm{g}$/ml농도에서 72시간 배양시 HeLa cell의 68%가 파괴되었으며 현미경 관찰 결과 세포 모양의 형태학적 변화가 일어났다. 정상세포주인 fibroblast에는 오매의 ethylacetate 및 hexane 추출물이 아무런 유의적 영향을 미치지 않았으며 현미경 관찰 시에도 변화가 일어나지 않았다

Negative Effects of Water Extracts from Pinus densiflora Sawdust on Mycelium Growth of the Shiitake Mushroom Lentinula edodes

  • Choi, Myung-Suk;Yang, Jae-Kyung
    • 한국약용작물학회지
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    • 제13권2호
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    • pp.110-114
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    • 2005
  • Extracts from sawdust of Pinus densiflora were showed antifungal activity against Lentinula edodes. It was extracted by hot water and then successively extracted by n-hexane, ethyl acetate, and methanol. The yields of the n-hexane-soluble, ethyl acetatesoluble, methanol-soluble and methanol-insoluble fractions of water extracts were 8.2%, 10.6%, 32.0%, and 49.2%, respectively. The ethyl acetate-soluble fraction showed the greatest antifungal activity against L. edodes: 41.5% inhibition at 1,000 ppm. However, there were not significant differences of antifungal activities between n-hexane-soluble fraction and methanol-soluble fraction at a concentration of 1,000 ppm. The hot water extracts showed 23.5% of antifungal activity against L. edodes at a concentration of 1000 ppm. The four antifungal compounds were separated from ethyl acetate fraction by thin layer chromatography.

단삼 추출물의 세포독성과 항균효과 (Cytotoxicity and Antimicrobial Effects of Extracts from Salvia miltiorrhiza)

  • 곽정숙;백승화
    • 생약학회지
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    • 제34권4호통권135호
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    • pp.293-296
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    • 2003
  • This study was carried out to evaluate cytotoxic effects of Salvia miltiorrhiza extracts on NIH 3T3 fibroblasts and KB cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The comparison of $IC_{50}$ of values of Salvia miltiorrhiza extracts in KB cell lines showed that their susceptibility to these extracts decreased in the following order: hexane extract > chloroform extract > methanol extract> dichloromethane extract > ethyl acetate extract>ethanol extract by the MTT method. The dried roots of Salvia miltiorrhiza was extracted several solvents, and then antimicrobial activity was investigated. The minimal inhibitory concentrations (MIC's) of the extract against microorganisms were also examined. Amtimicrobial activity of ketoconazol as reference was compared to those of extracts of hexane, chloroform, dichloromethane, ethyl acetate, ethanol and methanol. The antimicrobial activity of all extracts from the sample had growth inhibition activity against gram-negative bacteria, gram-positive bacteria and fungi. These results suggest that the hexane and chloroform soluble extracts of Salvia miltiorrhiza may be a valuable choice for the studies on the tumor cell lines and growth inhibition activity.

용매에 따른 카레 향신료 추출물의 항산화 효과 및 혼합효과 (Antioxidant and Mixture Effects of Curry Spices Extracts Obtained by Solvent Extraction)

  • 안채경;이영철;염초애
    • 한국식품과학회지
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    • 제32권3호
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    • pp.491-499
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    • 2000
  • 카레분의 단품향신료와 카레분(curry powder)을 메탄올, 에틸아세테이트, 헥산으로 추출하여 수율, 항산화 효과, 총페놀함량, 총토코페롤 함량을 측정하였다. 메탄올, 에틸아세테이트, 헥산의 추출 수율은 각각 $6.4{\sim}42.9$, $3.9{\sim}26.2$, $1.6{\sim}29.2\;%$로 극성이 높아질수록 수율이 증가하였다. 미강유의 각 용매별 추출물을 1,500ppm 첨가하여 Rancimat로 유도기간을 측정한 결과 메탄올 추출물은 rosemary, sage, ginger, turmeric, nutmeg 순으로 우수하였고, 에틸아세테이트 추출물은 rosemary, sage, oregano, thyme, ginger의 순이었으며 헥산 추출물은 rosemary, sage, oregano, ginger, thyme 순이었다. 1,500ppm을 미강유에 첨가하였을 때 BHT보다 유도기간이 길게 나타난 향신료는 3가지 용매 추출물에서 공통적으로 rosemary, sage, ginger, oregano, thyme이었으며 이들 향신료를 200, 500, 1,000, 1,500, 2,000 ppms 농도로 미강유에 첨가한 후 유도기간을 측정한 결과 rosemary와 sage는 헥산 추출물 2,000ppm에서, ginger는 메탄올 추출물 2,000ppm에서 우수한 효과를, oregano와 thyme은 에틸아세테이트 추출물이 1,500ppm과 2,000ppm에서 큰 차이 없이 우수한 효과를 나타내었다. Rosemary의 혼합에 따라 ginger와 thyme의 항산화 효과가 크게 증가함을 알 수 있었으나 상승효과라기 보다는 rosemary로 인한 유도기간의 연장 효과였다. 총페놀 함량은 메탄올 추출물은 $17,924{\sim}801\;mg%$, 에틸아세테이트 추출물은 $13,893{\sim}57\;mg%$, 헥산 추출물은 $6,864{\sim}12\;mg%$이었으며 총페놀함량과 항산화 효과가 일치하지는 않았다. 토코페롤의 경우 oregano가 3194.8mg%로 가장 높았으며, sage 1014.8 mg%, rosemary 571.1 mg%, thyme 187 mg%였고 ginger에서는 토코페롤이 검출되지 않았으며 토코페롤함량과 항산화 효과는 뚜렷한 상관관계가 발견되지 않았다.

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