• Title/Summary/Keyword: Heterotrophs

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The Basic Study for the Roof-Harvested Rainwater Quality Characteristics as a Function of Rainfall Duration (강우지속시간에 따른 건물지붕빗물의 성상특성변화에 대한 기초 연구)

  • Kim, Sung-Pyo;Kim, Doo-Cheol;Oh, Jun-Sik;Kim, Lee-Hyung;Min, Kyung-Suk
    • Journal of Wetlands Research
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    • v.14 no.1
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    • pp.11-20
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    • 2012
  • The aim of this study is to accumulate the data about the roof-harvested rainwater quality characteristics for the better rainwater management. According to the results of rainwater quality indicators (pH, COD, TSS, T-N, T-P, $NH_4$-N, $NO_3$-N, enteric bacteria, heterotrophs, Pb, Cd, Cu), the rainwater qualities of the first flush of roof-harvested was the most polluted about 2 or 3 times (more than 100 times in microbial contamination) compared to the qualities of directly collected rainwater. In the first 10 min flush, over the 60% amounts of contaminant were released from the roof during 30 minute rainwater monitoring. Through statistical factor analysis, relatively close items(variables) can be categorized. It is expected that the continuous monitoring of the roof-harvested rainwater will help to design the safe and economical rainwater storage system in Korea.

신경회로망을 이용한 순환식 돈분폐수 처리시스템의 모니터링

  • Choe, Jeong-Hye;Son, Jun-Il;Yang, Hyeon-Suk;Jeong, Yeong-Ryun;Lee, Min-Ho;Go, Seong-Cheol
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.125-128
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    • 2000
  • A recycling reactor system operated under sequential anoxic and oxic conditions for the swine wastewater has been developed, in which piggery slurry is fermentatively and aerobically treated and then part of the effluent recycled to the pigsty. This system significantly removes offensive smells (at both pigsty and treatment plant), BOD and other loads, and appears to be costeffective for the small-scale farms. The most dominant heterotrophs were Alcaligenes faecalis, Brevundimonas diminuta and Streptococcus sp. in order while lactic acid bacteria were dominantly observed in the anoxic tank. We propose a novel monitoring system for a recycling piggery slurry treatment system through neural networks. Here we tried to model treatment process for each tank(influent, fermentation, aeration, first sedimentation and fourth sedimentation tanks) in the system based on population densities of heterotrophic and lactic acid bacteria. Principle component analysis(PCA) was first applied to identify a relation between input(microbial densities and parameters for the treatment such as population densities of heterotrophic and lactic acid bacteria, suspended solids (SS), COD, $NH_3-N$, ortho-P, and total-P) and output, and then multilayer neural networks were employed to model the treatment process for each tank. PCA filtration of input data as microbial densities was found to facilitate the modeling procedure for the system monitoring even with a relatively lower number of input. Neural networks independently trained for each treatment tank and their subsequent combinatorial data analysis allowed a successful prediction of the treatment system for at least two days.

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Changing C-N Interactions in the Forest Floor under Chronic N Deposition: Implications for Forest C Sequestration

  • Park, Ji-Hyung
    • Journal of Ecology and Environment
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    • v.31 no.3
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    • pp.167-176
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    • 2008
  • Atmospheric N deposition has far-reaching impacts on forest ecosystems, including on-site impacts such as soil acidification, fertilization, and nutrient imbalances, and off-site environmental impacts such as nitrate leaching and nitrous oxide emission. Although chronic N deposition has been believed to lead to forest N saturation, recent evidence suggests that N retention capacity, particularly in the forest floor, can be surprisingly high even under high N deposition. This review aims to provide an overview of N retention processes in the forest floor and the implications of changing C-N interactions for C sequestration. The fate of available N in forest soils has been explained by the competitive balance between tree roots, soil heterotrophs, and nitrifiers. However, high rates of N retention have been observed in numerous N addition experiments without noticeable increases in tree growth and soil respiration. Alternative hypotheses have been proposed to explain the gap between the input and loss of N in N-enriched, C-limited systems, including abiotic immobilization and mycorrhizal assimilation, both of which do not require additional C sources to incorporate N in soil N pools. Different fates of N in the forest floor have different implications for C sequestration. N-induced tree growth can enhance C accumulation in tree biomass as observed across temperate regions. C loss from forests can amount to or outweigh C gain in N-saturated, declining forests, while another type of 'C-N decoupling' can have positive or neutral effects on soil C sequestration through hampered organic matter decomposition or abiotic N immobilization, respectively.

Nutrients removal and microbial activity for A2O Process Using Activated Sludge Models (활성슬러지 모델을 이용한 A2O공법 영양염류 제거 및 미생물 거동)

  • Yoon, Hyunsik;Kim, Dukjin;Choi, Bongho;Kim, Moonil
    • Journal of Korean Society of Water and Wastewater
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    • v.26 no.6
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    • pp.889-896
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    • 2012
  • In this study, simulation results of nitrogen and phosphorus removals and microbial activities for an $A_2O$ process in wastewater treatment plant are presented by using Activated Sludge Models (ASMs). Simulations were performed using pre-calibrated model and layout implemented in GPS-X simulation software. The models were used to investigate variations of SRT, water temperature, DO and C/N ratio effect on nutrients removal and microbial activity. According to the simulated results, the successful nitrification required SRT higher than 10.3 days, whereas increase of $NO_3$-N loading in the anaerobic reactor caused phosphorus release by PAOs; the effluent $NH_4$-N showed rapid change between $12^{\circ}C$(21.7 mg/L) and $13^{\circ}C$(3.2 mg/L); the effluent phosphorus was increased up to 1.9 mg/L at water temperature of $25^{\circ}C$; the DO increase was positive for heterotrophs and autotrophs growths but negative for PAOs growth; the PAOs showed low activity when C/N ratio was lower than 2.5. The experimental results indicated that the calibrated models can assure the prediction quality of the ASMs and can be used to optimize the $A_2O$ process.

Characteristics of Mercury-resistant Bacteria Isolated from River Water (하천에서 분리한 수은 내성세균의 특성)

  • 정현미;김상종;고영희
    • Korean Journal of Microbiology
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    • v.28 no.1
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    • pp.76-82
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    • 1990
  • In samples taken from mouth of the Nakdong River, mercury-resistant bacteria grown on the media supplemented with over 20 ppm of mercuric chlorice were below 0.3% of all aerobic heterotrophs. Among them, seven strains grown over 100 ppm of mercuric chloride were isolated and all were identified as Pseudomonas. The toxic effect of mercury on the growth of the most resistant strain N14 was influenced by the organic compounds and concentration. The growth and physiological activity to N14 strain were affected by toxic mercury in the early stage: The viable count and glucose turn over rate of N14 strain dropped to the lowest level as soon as the bacteria came into contact with mercury. During the extended lag period, however, bacteria accommodated to the stress and the viable count and glucose turnover rate increased. After the lag period, bacteria began to proliferate and their growth reached similar level to that of control. In crude extracts of N14 strain grown in nutrient browth containing. $10{\mu}M$ $HgCl_{2}$, a mercuric ion dependent oxidation of NADPH was demonstrated. Therefore the mechanism of mercury-resistance of the N14 strain involved the elimination of the mercury from growth media. In the N14 strain which a wide range of resistance to antibiotics was observed in, four multiple plasmids were detected. As a result, the supposition that N14 strain has a plasmid-encoded enzyme system may be quite within the realms of possobility.

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Modeling of Recycling Oxic and Anoxic Treatment System for Swine Wastewater Using Neural Networks

  • Park, Jung-Hye;Sohn, Jun-Il;Yang, Hyun-Sook;Chung, Young-Ryun;Lee, Minho;Koh, Sung-Cheol
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.5
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    • pp.355-361
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    • 2000
  • A recycling reactor system operated under sequential anoxic and oxic conditions for the treatment of swine wastewater has been developed, in which piggery slurry is fermentatively and aerobically treated and then part of the effluent is recycled to the pigsty. This system significantly removes offensive smells (at both the pigsty and the treatment plant), BOD and others, and may be cost effective for small-scale farms. The most dominant heterotrophic were, in order, Alcaligenes faecalis, Brevundimonas diminuta and Streptococcus sp., while lactic acid bacteria were dominantly observed in the anoxic tank. We propose a novel monitoring system for a recycling piggery slurry treatment system through the use of neural networks. In this study, we tried to model the treatment process for each tank in the system (influent, fermentation, aeration, first sedimentation and fourth sedimentation tanks) based upon the population densities of the heterotrophic and lactic acid bacteria. Principal component analysis(PCA) was first applied to identify a relationship between input and output. The input would be microbial densities and the treatment parameters, such as population densities of heterotrophic and lactic acid bacteria, suspended solids(SS), COD, NH$_4$(sup)+-N, ortho-phosphorus (o-P), and total-phosphorus (T-P). then multi-layer neural networks were employed to model the treatment process for each tank. PCA filtration of the input data as microbial densities was found to facilitate the modeling procedure for the system monitoring even with a relatively lower number of imput. Neural network independently trained for each treatment tank and their subsequent combined data analysis allowed a successful prediction of the treatment system for at least two days.

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Report of 22 unrecorded bacterial species in Korea belonging to phylum Bacteroidetes, discovered during surveys in 2018

  • Kim, Min Ji;Kim, Yeong Seok;Cha, Chang-Jun;Im, Wan-Taek;Jeon, Che Ok;Joh, Kiseong;Seong, Chi Nam;Yi, Hana;Kim, Seung Bum
    • Journal of Species Research
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    • v.9 no.1
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    • pp.26-34
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    • 2020
  • The phylum Bacteroidetes covers phenotypically diverse groups of Gram negative rods that do not form endospores, and currently includes 6 classes, 6 orders, 33 families and 380 genera. Members of Bacteroidetes can be aerobic and anaerobic heterotrophs, hydrogen utilizing chemolithotrophs, or methylotrophs. They can be isolated from diverse habitats including terrestrial and aquatic environments, environments with extreme physicochemical conditions, and animal and plant hosts. During a series of extensive surveys of prokaryotic species diversity in Korea, bacterial strains belonging to Bacteroidetes were isolated from various sources of aquatic and terrestrial environments. A total of 22 isolates were obtained, which represent 22 unrecorded species in Korea belonging to 14 genera of 6 families. Sixteen species among them were assigned to Flavobacteriaceae, two species were to Sphingobacteriaceae, and single species was to each of the families Bacteroidaceae, Balneolaceae, Chitinophagaceae and Cytophagaceae. At genus level, Chryseobacterium (5 species) and Flavobacterium (5 species) were the most abundant genera, and single species were obtained for the genera Bacteroides, Baloneola, Terrimonas, Dyadobacter, Aquimarina, Arenibacter, Gillisia, Gilvibacter, Salinimicrobium, Winogradskyella, Pedobacter and Sphingobacterium. The detailed descriptions of each unrecorded species are provided.

The Nutrient Removal of Mixed Wastewater composed of Sewage and Stable Wastewater using SBR (SBR을 이용한 하수와 우사폐수로 구성된 혼합폐수의 영양소 제거)

  • 김홍태
    • Journal of Environmental Science International
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    • v.8 no.5
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    • pp.617-623
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    • 1999
  • This study was carried out to obtain the optimal operating parameter on organic matters and nutrient removal of mixed wastewater which was composed of sewage and stable wastewater using SBR. A laboratory scale SBR was operated with An/Ae(Anaerobic/Aerobic) ratio of 3/3, 2/4 and 4/2(3.5/2.5) at organic loading rate of 0.14 to 0.27 kgBOD/$m^3$/d. TCOD/SCOD ratio of mixed wastewater was 3, so the important operating factor depended upon the resolving the particulate parts of wastewater. Conclusions of this study were as follows: 1) For mixed wastewater, BOD and COD removal efficiencies were 93-96% and 85-89%, respectively. It was not related to each organic loading rate, whereas depended on An/Ae ratio. During Anarobic period, the amount of SCOD consumption was very little, because ICOD in influent was converted to SCOD by hydrolysis of insoluble matter. 2) T-N removal efficiencies of mixed wastewater were 55-62% for Exp. 1, 66-76% for Exp. 2, and 67-81% for Exp. 3, respectively. It was found that nitrification rate was increased according to organic concentration in influent increased. Therefore, the nitrification rate seemed to be achieved by heterotrophs. During anoxic period, denitrification rate depended on SCOD concentration in aerobic period and thus, was not resulted by endogenous denitrification. However, the amount of denitrification during anaerobic period were 3.5-14.1 mg/cycle, and that of BOD consumed were 10-40 mg/cycle. 3) For P removal of mixed wastewater, EBPR appeared only Mode 3($3^*$). It was found that the time in which ICOD was converted to VFA should be sufficient. For mode 3 in each Exp., P removal efficiencies were 74, 87, and 81%, respectively. But for 45-48 of COD/TP ratio in influent, P concentration in effluent was over 1 mg/L. It was caused to a large amount of ICOD in influent. However, as P concnetration in influent was increased, the amounts of P release and uptake were increased linearly.

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Estimation of Net Community Production Based on O2/Ar Measurements (O2/Ar 관측에 기반한 순군집생산량 추정 연구 동향)

  • HAHM, DOSHIK;LEE, INHEE
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.23 no.1
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    • pp.49-62
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    • 2018
  • Net community production (NCP), defined as the difference between net primary production and respiration of heterotrophs, has been used as a measure of oceanic biological carbon pump. This paper summarizes the theoretical background and experimental methods for the estimation of NCP based on $O_2/Ar$ measurements ($O_2/Ar-NCP$). The high frequency measurements of $O_2/Ar-NCP$ (<1 min) is a significant enhancement over the conventional measures of biological pump, such as new production and export production. This paper also introduces some of important works as to the comparison between $O_2/Ar-NCP$ and other measures of biological pump, the distributions of $O_2/Ar-NCP$ in the oceans, and the correlation of $O_2/Ar-NCP$ with various oceanic parameters, including community structures.

Holocarpic oomycete parasites of red algae are not Olpidiopsis, but neither are they all Pontisma or Sirolpidium (Oomycota)

  • Giuseppe C. Zuccarello;Claire M. M. Gachon;Yacine Badis;Pedro Murua;Andrea Garvetto;Gwang Hoon Kim
    • ALGAE
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    • v.39 no.1
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    • pp.43-50
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    • 2024
  • Oomycetes are ubiquitous heterotrophs of considerable economic and ecological importance. Lately their diversity in marine environments has been shown to be greatly underappreciated and many lineages of intracellular holocarpic parasites, infecting micro- and macro-algae, remain to be fully described taxonomically. Among them, pathogens of marine red algae have been studied extensively as they infect important seaweed crops. Throughout the 20th century, most intracellular, holocarpic biotrophic oomycetes that infect red algae have been assigned to the genus Olpidiopsis Cornu. However, 18S rRNA sequencing of Olpidiopsis saprolegniae, the species considered the generitype for Olpidiopsis, suggests that this genus is not closely related to the marine pathogens and that the latter requires a nomenclatural update. Here, we compile and reanalyze all recently published 18S rRNA sequence data for marine holocarpic oomycetes, with a particular focus on holocarpic pathogens of red algae. Their taxonomy has been revised twice over the past four years, with suggestions to transfer them first into the genus Pontisma and then Sirolpidium, and into a monogeneric order, Pontismatales. We show however, that previously published topologies and the proposed taxa Pontisma, Sirolpidium, and Pontismatales are unsupported. We highlight that name changes that are unfounded and premature create confusion in interested parties, especially concerning pathogens of marine red algae that infect important seaweed crops. We thus propose that the names of these holocarpic biotrophic parasites of red algae are retained temporarily, until a supported topology is produced with more genetic markers to enable the circumscription of species and higher-level taxa.