• Journal of Microbiology and Biotechnology
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• 제11권5호
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• pp.899-901
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• 2001

Comparison of the abundances of heterotrophic bacteria in the East and South China Seas by stanctard epifluorescence was miscounted as heterotrophic bacteria in DAPI stained samples. This could result in 5-31% oversestimations of heterotrophic bacterial abundance in the study areas.

• 한국환경과학회지
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• 제10권3호
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• pp.247-252
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• 2001

This study was performed to evaluate the inactivation and microbial regrowth of heterotrophic and nitrifying bacteria using chloramine as a secondary disinfectant for drinding water distribution system. Three sets of the three reactors filled with the $Cl_2/NH_3-N$ ratio of 3:1, 4:1, and 5:1 were used in these experiments. Chloramine concentration were applied to each set of the reactors with $1mg/\ell$,\;2mg/\ell\;and\;3mg/\ell$, respectively. For the set with elapsed time and reached to zero level after 7 days. Heterotrophic bacteria remarkably increased and nitrification through the experimenatal period (21 day). Furthermore the regrowth of heterotrophic bacteria and nitrification were not found. More than $2mg/\ell$ of chloramine with $Cl_2/NH_3-N$ ratio of 3:1, the nitrification could be inhibited by 2 days of contact time.

• ALGAE
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• 제32권1호
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• pp.47-55
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• 2017

We explored feeding by the mixotrophic ciliate Mesodinium rubrum, heterotrophic nanoflagellates (HNFs), and small ciliates (<$30{\mu}m$ in cell length) on natural populations of heterotrophic bacteria in Masan Bay, Keum River Estuary, and in the coastal waters of the Saemankeum area, Korea when M. rubrum red tides occurred. We also measured ingestion rates of M. rubrum on cultured heterotrophic bacteria as a function of bacterial concentration in the laboratory. The ingestion rates of M. rubrum on natural populations of heterotrophic bacteria (2.3-16.8 bacteria $grazer^{-1}h^{-1}$) were comparable to or lower than those of co-occurring HNFs (10.7-41.7 bacteria $grazer^{-1}h^{-1}$), but much lower than those of co-occurring small ciliates (76.0-462.2 bacteria $grazer^{-1}h^{-1}$). However, the maximum grazing coefficient of M. rubrum ($0.245d^{-1}$) on natural populations of heterotrophic bacteria was much higher than that of small ciliates ($0.089d^{-1}}$), and slightly higher than that of HNFs ($0.204d^{-1}$). With increasing bacterial concentrations, ingestion rates of M. rubrum on cultured heterotrophic bacteria continuously increased, but became saturated at higher prey concentrations over $1-5{\times}10^6cells\;mL^{-1}$. The maximum ingestion rate of M. rubrum on cultured heterotrophic bacteria was 34.4 bacteria $grazer^{-1}h^{-1}$. Based on the present study, it is suggested that M. rubrum may be an important grazer of heterotrophic bacteria and sometimes have considerable grazing impact on natural populations of heterotrophic bacteria.

• 환경생물
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• 제22권1호
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• pp.75-82
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• 2004

2000년부터 2002년까지 통영 바다목장 해역 5개 정점표층수와 저층수에서 종속영양세균의 개체수를 평판계수법으로 측정하여 종속영양세균군집의 계절별 변동추이를 비교 분석하고, 우점종을 선별하여 동정하였다. 7월과 8월의 하절기에 채수한 해수에는 봄 혹은 가을 및 겨울에 채수한 해수에 비하여 모든 정점에서 훨씬 더 많은 종속영양세균이 검출되었다. 저층수에 비하여 표층수에서 종속영양세균의 수가 더 많았으나 동절기(2001년 12월과 2002년 2월)에는 해수 온도가 낮으며 해수의 대류가 느려짐에 따라 부유물질이 저층에 축적되는 관계로 저층수의 종속영양세균의 수가 표층수의 경우 보다 오히려 더 많이 검출되었다 해수 내 종속영양세균의 수와 태풍의 빈도는 뚜렷한 상관관계를 보이지 않아 태풍의 빈도 이외에 태풍의 진로와 강도 및 태풍을 수반하는 집중 호우와 해수의 온도 변화가 종속영양세균의 수에 영향을 미치는 것으로 판단되었다. 통영 바다목장 해역의 종속영양세균의 우점종은 Pseudomonas fluorescens, Pseudomonas stutzeri, Acinetobucter lwoffii 및 Sphingomonas paucimobilis로 동정되었다.

• 한국토양비료학회지
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• 제20권1호
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• pp.69-76
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• 1987

The aerobic heterotrophic bacteria in the histosphere associated with grasses (Gramineae, Caryphyllaceae, Crucifereae) and rice cultivars in saline and reclaimed saline paddy soils were varied with species and rice cultivars. The fraction of aerobic heterotrophic $N_2$-fixing bacteria to the total aerobic heterotrophic bacteria were averaged to eighteen percent in the histosphere of grasses and rice. Acetylene reducing activity of these bacteria were ranged from 1 to 24 n mole/tube/hr. Most of the bacteria strains were predominated of hydrogen utilizing bacteria. The majority of these bacteria were closed to Pseudomonas, Azospirillum, Klebsiella and Agrobacter.

• Ocean and Polar Research
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• 제29권4호
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• pp.401-410
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• 2007

국내에서 해양원생동물의 연구는 1990년대 초반에 본격적으로 시작되어 주로 원생동물의 분포와 섭식 등에 관한 연구가 진행되어왔다. 본 논문에서는 마산만 표영 환경에서의 박테리아와 원생동물의 분포, 박테리아와 원생동물의 상호작용과 원생동물과 식물플랑크톤의 상호작용의 특성에 대해 알아보고 앞으로의 연구방향에 대해 토의하였다. 마산만에서 종속영양 미소편모류의 현존량은 평균 $1.2{\times}10^3\;cells\;mL^{-1}$, 종속영양 와편모류는 평균 $7.9{\times}10^4\;cells\;mL^{-1}$, 섬모충류는 평균 $4.0{\times}10^4\;cells\;L^{-1}$로 나타났으며 전반적으로 엽록소-a 농도, 박테리아와 원생동물의 현존량은 유기물 유입이 많은 내만 정점에서 높게 나타났다. 종속영양 미소편모류는 박테리아 이차생산의 약 69%를 제거하는 것으로 나타나 박테리아 생물량을 조절할 것으로 판단되며 소형부유동물은 미소조류 및 박테리아와 양의 상관관계를 보였고 식물플랑크톤에 대한 섭식율(평균 24%)로 인해 이들 생물군집들에게 영향을 주었을 것으로 추정된다. 따라서 마산만에서 원생동물은 박테리아와 미소조류의 성장을 조절하고 이들의 생물량을 상위영양단계로 직접 전달하는 것으로 사료된다. 특히 종속영양 미소편모류는 마산만에서 박테리아의 주 포식자일 것으로 추정된다. 또한 마산만은 후생동물 먹이망보다 미세생물 먹이망이 상당히 잘 발달된 곳으로 추정된다.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2001년도 Proceedings of the 3rd Annual Meeting of Yellow Sea Environment
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• pp.6-10
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• 2001

Distribution characteristics, variation patterns and affecting factors of hetorotrophic bacteria were studied from April to May 1999 in Bohai Sea by standard Acridine Orange epifluorescence microscopy (AO method). The biomass in surface waters showed a small day-night variation, varying from 0.13-2.51$\mu\textrm{m}$$.$dm$\^$-3/ with an average of 0.84 $\mu\textrm{m}$$.$dm$\^$-3/. The biomass in bottom waters showed, however, a large variation, changing from 0.15-4.18 $\mu\textrm{m}$$.$dm$\^$-3/ with an average of 1.36 $\mu\textrm{m}$$.$dm$\^$-3/. The peak values were obtained at 5 and 11 am. The bottom water biomass showed a significant correlation with particulate organic carbon (r=0.639, p<0.05). Heterotrophic bacteria showed high biomass in nearshore waters and low values in offshore areas with a high biomass zone around Yellow Sea river mouth, which was consistent with the distribution of nutrients. The vertical distribution of heterotrophic bacteria showed biomass in bottom waters was higher than in surface water. The biomass of heterotrophic bacteria in Bohai Sea was similar with that in other marine waters.

• 미생물학회지
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• 제25권2호
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• pp.137-143
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• 1987

Dominant genera of heterotrophic bacteria were investigated seasonally in Kum River estuarine sediments. Numerical taxonomy on the isolated heterotrophic bacteria were carried out according to their morphological, physiological, nutritional and biochemical characters. Heterotrophic bacteria in the depths of 0 to 10 cm of sampled sediments were almost facultative anaerobes, and 77.9% to 86.9% of them were Gram negative rods. Kominant genera among the isolates were Pseudomonas, Flavobacterium, Micrococcus, Cnterobacteria and Vibrio during sampling periods. According to their character analyses by similarity index(S), the isolates were clustered in fourteen groups at clay type sediments (Site 1) and four groups at sand type sediments (Site 2).

• 미생물학회지
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• 제24권3호
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• pp.308-316
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• 1986

Vertical distribution of heterotrophic bacteria and physico-chemical characteristics were measuted in Kum River estuarine sediments. And interrelationship between heterotrophic bacterka and environmental factors was also studied. The type of sediment of Site 1 was silty clay, and sand at Site 2. Annual pH ranges were between 7.1 and 7.7 in the clay type sediment (Site 1) and 6.9-7.2 in the sand type sediment (Site 2). It was shown that organic matter contents were higher in the clay type sediment than those of sand type sediment. Redox potential values of sediments were decreased rapidly with depth at Site 1, but those of Sete 2 showed vertical fluctuation. Nitrogens(ammonia+amino acid-N, nitrate-N, nitrite-N) and phosphate in the clay type sediment showed higher values than those of sand type sediment. Annual distribution of heterotrophic bacteria were ranged $6.71{\times}10^4$ cells/g dry wt. $-2.50{\times}10^6$ cells/g dry wt. In the clay type sediment and $2.67{\times}10^3$ cells/g dry wt. $-1.94{\times}10^6$ cells/g dry wt. in the sand type sediment. Distribution of proteolytic, lipolytic, and amylolytic bacteria were decreased with the depth and the highest density was found in April and the lowest in January. Bacterial populations in sediments were closely correlated with such environmental factors as pH, redox potential, moisture content, organic matter contents, and inorganic nutrients such as nitrite-N and phosphate-P.

• 환경생물
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• 제24권3호
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• pp.258-267
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• 2006

본 연구는 경상남도 사천시 삼천포 항을 중심으로 인근의 40개의 조사정점을 선정하여 해수와 저질토에서 해양종속영양세균, E. coli, 그리고 Salmonella와 Shigella의 분포를 2003년 7월부터 12월가지 3차에 걸쳐 실시하였다. 본 연구의 조사기간(2003년 $7{\sim}12$월)중 삼천포만 인근해역의 조사정점에서 해양 종속영양세균의 분포는 $1.7{\pm}0.9{\times}10^3{\sim}2.4{\pm}0.9{\times}10^5CFUmL^{-1}$의 범주에서 변화하였으며, 정점 34에서 9월에 가장 높은 값을 나타냈다. 전반적으로 9월이 7월과 12월에 비해 모든 정점에서 다소 높은 값을 보였다. 저질토에서 혐기성 세균의 분포는 $2.2{\pm}0.2{\times}10^3{\sim}2.0{\pm}0.2{\times}10^5CFUg^{-1}dry$ wt.의 범주에서 변화하였다. 혐기성 세균의 경우 외해 정점에서는 내만에 위치한 정점들보다 년 중 낮은 균체수를 유지하고 있어 물리, 화학적인 환경요인들에 의해 별 영향인 없음을 알 수 있다. E. coli의 분포는 해수의 경우, 7월중에만 사천만 인근(St. 1, 2, 3, 4),진주만과 창선도 인근 (St. 15, 23, 31), 삼천포항 인근(St. 14)에서 검출되고 9월과 12월에는 전 정점에서 전혀 검출되지 않았다. 저질토의 경우, 7월에 사천만 인근(St. 1, 2)과 창선도 인근(St. 16, 31)에서만 검출 되었다. Salmonella와 Shigella의 분포는 해수와 퇴적토에서 7월과 9월에 일부 해역에서만 검출되었다.