• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1060-1067
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• 2006

This study was undertaken to determine the effect of reservoirs on water quality and the distribution of pathogenic and indicator bacteria in a drinking water distribution system (total length 14km). Raw water, disinfected water, and water samples from the distribution system were subjected to physicochemical and microbiological analyses. Most factors encountered at each season included residual chloride, nitrate, turbidity, and phosphorus for heterotrophic bacterial distribution, and hardness, heterotrophic bacteria, sampling site, and DOC (dissolved organic carbon) for bacteria on selective media. No Salmonella or Shigella spp. were detected, but many colonies of opportunistic pathogens were found. Comparing tap water samples taken at similar distances from the water treatment plant, samples that had passed through a reservoir had a higher concentration of heterotrophic bacteria, and a higher rate of colony formation with 10 times as many bacteria on selective media. Based on the results with m-Endo agar, the water in reservoirs appeared safe; however, coliforms and opportunistic pathogenic bacteria such as Pseudomonas aeruginosa were identified on other selective media. This study illustrates that storage reservoirs in the drinking water distribution system have low microbiological water quality by opportunistic pathogens, and therefore, water quality must be controlled.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.858-868
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• 2009

The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic water layer during summer stratification periods. Image analysis techniques with fluorescent in situ hybridization (FISH) as well as [$^3H$]-methyl-thymidine incorporation methods were applied to analyze differences in the composition and activity of bacterial communities. The greatest differences in trophic parameters were found between the humic lake and remaining non-humic ones. The same bacterial and heterotrophic nanoflagellate (HNF) cell size classes dominated in all the studied lakes. However, distinct increases in the contributions of large bacterial (>$1.0{\mu}m$) and HNF (>$10{\mu}m$) cells were observed in eutrophic lakes. The bacterial community was dominated by the ${\beta}$-Proteohacteria group, which accounted for 27% of total DAPI counts. Ciliate communities were largely composed of Oligotrichida. Positive correlations between bacteria and protists, as well as between nanoflagellates (both heterotrophic and autotrophic) and ciliates, suggest that concentrations of food sources may be important in determining the abundance of protists in the studied lakes.

• Journal of the Korea Organic Resources Recycling Association
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• v.13 no.2
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• pp.121-127
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• 2005

A heterotrophic nitrifying bacterium was isolated from the compost and analyzed for its characteristics. This bacterium was found to be a Gram positive rod, catalase positive, and motile. Nitrite production was detected on the ammonium acetate medium through the violet color formation. BBL test showed that this strain has high homology with Bacillus strains. Phylogenetic analysis using 16S rDNA revealed that the bacterium has 94% of similarity with Mycobacterium smegmatis strain.

• Proceedings of the Korean Environmental Health Society Conference
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• 2003.06a
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• pp.136-139
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• 2003

This study is to observe the occurrence of heterotrophic bacteria in terms of free chlorine residuals in two different water distribution system which belongs to both K and Y water treatment plant of S city of Korea. The data analyzing in distribution systems (DS) shows that the free chlorine residuals decrease from 0.10 to 0.56 mgmg/L for K, and 0.51 to 0.78 mg/L for Y. The decay of free chlorine is clearly higher in both March and August than those of in January. The HPC in DS are ranged from 0 to 40 CFU/mL for K, 0 to 270 CFU/mL for Y, on R2A medium. In particular, its level is relatively high at consumers ground storage tanks, taps and point-of-end area of Y. The predominant genera is studied in distribution systems are Acinetobacter, Sphingomonas (branch of Pseudomonas), Micrococcus, Bacillus, Staphylococcus. The diversity of heterotrophic bacteria increase in the end-point area. Most of them are either encapsulated cells or cocci of gram-positve. In conclusion, the point-of-end area in distribution systems shows the longer flow distance from water treatment plants, the greater diversity and higher level of heterotrophic bacteria due to the significant decay of free chlorine residuals.

• Journal of the korean society of oceanography
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• v.37 no.2
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• pp.58-65
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• 2002

The study of microbial communities in sediments is impaired by the lack of reliable extraction methods. This study reports on the efficiency of recovery of a method for extracting bacteria, protists and diatoms from sandy sediments using a modified decant/fix method. The best extractions were achieved after fixation with a microtubule stabilising fixative and subsequent sonication for 80 seconds. We estimate that the efficiencies of recovery of bacteria and mixed heterotrophic flagellates were $96.4{\pm}3.5\%$ and $96.9{\pm}4.6\%$, respectively. Diatoms were recovered with an efficiency of 38-83% and varied considerably from species to species. This study suggests that the decant/fix method is effective in extracting small cells such as bacteria and heterotrophic flagellates, and that the efficiency of recovery of the method varies due to cell length and different types of organisms. When microbial carbon biomass had been underestimated by up to 32%, with much of that relating to larger cells such as microalgae and ciliates. We note that the corrected abundances may be still a subset of the total numbers present.

• Journal of Species Research
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• v.9 no.4
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• pp.448-454
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• 2020

Nine free-living heterotrophic flagellates were cultured from marine intertidal sediments and freshwater sediments from Korea. These species are described with uninterpreted records based on light microscopy of living cells and reported taxonomically for the first time from Korea. Diagnostics of these species are as follows; Notosolenus hemicircularis: 9-11.8 ㎛ long with flagellar reservoir, ventrally flattened and dorsally convex with hyaline semicircular collar around short anterior neck, and 8 ridges on cell surface. Thecamonas tranhens: 4.5-7.1 ㎛ long, plastic with proboscis comprising an anterior flagellum surrounded by membranous sleeve. Bodomorpha minima: 4.5-7.0 ㎛ long, rigid with small rostrum in anterior end and active anterior flagellum. Cercomonas hiberna: 5.6-10.9 ㎛ long, very plastic with pseudopodia, cytoplasmic strand and 1 or 2 contractile vacuoles. Cercomonas pellucida: 7.5-13 ㎛ long, plastic with pseudopodia, cytoplasmic strand and single contractile vacuole. With nucleus closely connected to basal bodies. Eocercomonas echina: 4.7-6.5 ㎛ long, plastic with pseudopodia, cytoplasmic strand and 1 or 2 contractile vacuoles. Paracercomonas astra: 5.7-7.3 ㎛ long, moderately metabolic with pseudopodia, cytoplasmic strand and 1 or 2 contractile vacuoles. Paracercomonas minima: 5-9 ㎛ long, metabolic with pseudopodia, cytoplasmic strand and single contractile vacuole. Paracercomonas producta: 6.1-9.9 ㎛ long, very metabolic with pseudopodia, long cytoplasmic strand and single contractile vacuole.

• Korean Journal of Microbiology
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• v.28 no.3
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• pp.204-209
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• 1990

The total and heterotrophic bacterial distributions, compositions and alkaline phosphatese actibities were analyzed in Lake Soyang from Sep. 1987 to Aug. 1988. The heterotrophic bacteria was small portion, 0.07-2.63% of total bacterial number which ranged from $3.2{\times}10^{5}$ to $3.2{\times}10^{6}$ cells/${\mu}\ell$. The composition of bacterial community was less diverse in summer and at the fish farm site and Peridinium blooming site. Pseudomonas and Flavo bacterium were the dominant genera in all sites. The highest proportion and activity of alkaline phsophatase was appeared in Flavobacterium, while Pseudomonas was the most predominant group.

• Journal of Soil and Groundwater Environment
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• v.27 no.3
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• pp.41-49
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• 2022

This study utilized citric acid as a floating agent in biological denitrification process and assessed its role under different carbon supplying conditions. Several microcosm tests including citric acid active (CAA), precipitating tablet release active (PTRA) and floating tablet release active (FTRA) were conducted to evaluate nitrate denitrification efficacy. In CAA reactors, nitrate removal was accompanied by the formation of denitrification by-products such as nitrite and nitrous oxide, with the extent of nitrate removal being proportional to citric acid concentration. These results suggest that citric acid induced heterotrophic biological denitrification. PTRA reactor that incorporated CAA and the same electron donor showed a similar denitrification efficiency to CAA reactor. FTRA reactor, which contained the same amount of fumarate as PTRA, enhanced denitrification by 7% as compared to the PTRA reactor. The overall results of this work indicate that surplus citric acid can be efficiently utilized in heterotrophic denitrification.

• Applied Chemistry for Engineering
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• v.33 no.2
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• pp.222-228
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• 2022

As a possible feedstock for biodiesel, the marine green alga Tetraselmis sp. was cultivated under different conditions of phototrophic, mixotrophic and heterotrophic cultures. Glycerol, a byproduct from biodiesel production process, was used as the carbon source of mixotrophic and heterotrophic culture. The effects of glycerol supply and nitrate-repletion were compared for different trophic conditions. Mixotrophic cultivation exhibited higher biomass productivity than that of phototrophic and heterotrophic cultivation. Maximum lipid productivity of 55.5 mg L^-1 d^-1 was obtained in the mixotrophic culture with 5 g L^-1 of glycerol and 8.8 mM of nitrate due to the enhancement of both biomass and lipid accumulation. The major fatty acid methyl esters (FAME) in the produced biodiesel were palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3). The degree of unsaturation was affected by different culture conditions. The biodiesel properties predicted by correlation equations based on the FAME profiles mostly complied with the specifications from the US, Europe and Korea, with the exception of the cold-filter plugging point (CFPP) criterion of Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.3
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• pp.286-293
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• 1983

The glucose application remarkably increased the heterotrophic acetylene reducing activities during one month incubation. The amount of the increases varied between the soils. Application of rice straw brought about the significant increases during incubation time. Compost contained the largest amount of available-N among the C-sources, and thereby brought the smallest increase in all soils. The cumulative fixed nitrogen with application of rice straw at 30 days incubation in the three experimented soils were highest in Hwadong clay soil, i.e, $2.2mg^N/100g$, intermediate in Ratchaburi soil $0.85mg^N$, and least in Konosu soil $0.80mg^N/100g$. On the other hand, nitrogen fixing heterotrophic, bacteria, such as Clostridia, aerobes and anaerobes, were remarkably increased by application of rice straw while Azotobacter and Beijerinkia were not. The cumulative fixed nitrogen was more pronounced in the clay soil than in the coarse loamy soil. More pronounced nitrogen fixing activities in light condition(heterotrophic + photosynthetic) than that in dark(heterotrophic) condition have been observed both in the coarse loamy and clay soils. The nitrogen fixing ability of photosynthetic microbes in paddy soil is probably higher in coarse loamy soil than in clay soil.