• International Journal of Industrial Entomology and Biomaterials
• /
• 제9권1호
• /
• pp.137-144
• /
• 2004

The diamondback moth, Plutella xylostella, is notorious because of its extensive potential and actual dispersal ability. Previously, the Korean populations of P. xylostella was extensively collected and analyzed for their genetic population structure using a portion of mitochondrial DNA (mtDNA). One of the postulated characteristics on population genetic structure of the species includes the presence of heterogeneous haplotypes, possibly possessed by some dispersed ones from neighboring countries. In this study, we sequenced ten P. xylostella collected from China (∼2,000 km away from the middle part of Korea) to know the genetic relationships of these to the Korean P. xylostella. Sequence analysis of the identical portion of COI gene resulted in five haplotypes with the sequence divergence ranging from 0.5% (two nucleotides) to 1.1 % (five nucleotides) among them and from 0.7% (three) to 2.5% (11) to the pre-existing 52 Korean haplotypes. Phylogenetic analysis showed that the Chinese P. xylostella were neither clearly separated from the Korean haplotypes nor clustered with one heterogeneous Korean haplotype. This result reinforces the significance of gene flow in this species and suggests to exclude the possibility that the heterogeneous Korean haplotypes may have emigrated from China, where our samples were obtained, although further extensive investigation is required.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권8호
• /
• pp.3989-3995
• /
• 2016

Background: Childhood acute lymphoblastic leukemia (ALL) is a heterogeneous genetic disease and its etiology remains poorly understood. Recent genome wide association and replication studies have highlighted specific polymorphisms contributing to childhood ALL predispositions mostly in European populations. It is unclear if these observations generalize to other populations with a lower incidence of ALL. The current case-control study evaluated variants in ARID5B (rs7089424, rs10821936), IKZF1 (rs4132601) and CEBPE (rs2239633) genes, which appear most significantly associated with risk of developing childhood B-lineage ALL. Materials and Methods: Using TaqMan assays, genotyping was conducted for 162 de novo B-lineage ALL cases and 150 unrelated healthy controls in India. Appropriate statistical methods were applied. Results: Genotypic and allelic frequencies differed significantly between cases and controls at IKZF1-rs4132601 (p=0.039, p=0.015) and ARID5B-rs10821936 (p=0.028, p=0.026). Both rs10821936 (p=0.019; OR 0.67; 95% CI=0.47-0.94) and rs4132601 (p=0.018; OR 0.67; 95%CI 0.48-0.94) were associated with reduced disease risk. Moreover, gender-analysis revealed male-specific risk associations for rs10821936 (p=0.041 CT+CC) and rs4132601 (p=0.005 G allele). Further, ARID5B-rs7089424 and CEBPE-rs2239633 showed a trend towards decreased disease risk but without significance (p=0.073; p=0.73). Conclusions: Our findings provide the first evidence that SNPs ARID5B-rs10821936 and IKZF1-rs4132601 are associated with decreased B-lineage ALL susceptibility in Indian children. Understanding the effects of these variants in different ethnic groups is crucial as they may confer different risk of ALL within different populations.

• Journal of Yeungnam Medical Science
• /
• 제37권1호
• /
• pp.13-21
• /
• 2020

Maturity-onset diabetes of the young (MODY) is a clinically heterogeneous group of monogenic disorders characterized by ß-cell dysfunction. MODY accounts for between 2% and 5% of all diabetes cases, and distinguishing it from type 1 or type 2 diabetes is a diagnostic challenge. Recently, MODY-causing mutations have been identified in 14 different genes. Sanger DNA sequencing is the gold standard for identifying the mutations in MODY-related genes, and may facilitate the diagnosis. Despite the lower frequency among diabetes mellitus cases, a correct genetic diagnosis of MODY is important for optimizing treatment strategies. There is a discrepancy in the disease-causing locus between the Asian and Caucasian patients with MODY. Furthermore, the prevalence of the disease in Asian populations remains to be studied. In this review, the current understanding of MODY is summarized and the Asian studies of MODY are discussed in detail.

• 한국양식학회지
• /
• 제10권3호
• /
• pp.321-326
• /
• 1997

The random amplified polymorphic DNA (RAPD) technique was used to anlayze six isolates of two species of Porphyra, P. yezoensis and P. tenera. Four 21-mer prrmers were combined randomly into six groups of double primers and screened for DNA amplification using nuclear and chloroplast tempate DNA. The RAPD patterns resulting from RnRc and CnCc primers provided evidence for both genetically homo-and heterogeneous populations of P. yezoensis and P. tenera. Similarity values obtained by RnRc primer analysis of nuclear DNA varied from 0.364 to 0.714 and those of chloroplast DNA were high, ranging from 0.727 to 1.000, except for P. yezoensis (Enoura).

• 한국정보과학회:학술대회논문집
• /
• 한국정보과학회 2006년도 한국컴퓨터종합학술대회 논문집 Vol.33 No.1 (A)
• /
• pp.46-48
• /
• 2006

현재 각 분야에서 다양한 실험 데이터가 산출되면서 이종(heterogeneous) 데이터간의 상관관계 분석에 대한 중요성이 더욱 부각되고 있다. 특히, 대규모 실험에 의해 급속하게 증가하고 있는 대량의 바이오 데이터에서 이런 문제를 해결하기 위한 새로운 데이터 마이닝 방법이 요구된다. 본 논문은 특성이 다른 두 데이터 셋에서 서로 상관관계가 있는 부분 패턴을 파악할 수 있는 새로운 알고리즘을 제안한다. 제안한 알고리즘은 다중 개체 집단을 유지하면서 상호간 공진화하는 확률적 진화컴퓨팅 방법에 기반하고, 전체의 탐색 포인트들을 분해하여 최적해를 찾는 점에서 장점을 가지고 있다. 실험 결과, 본 논문에서는 효모 유전자에 대한 발현 데이터와 모티프 데이터의 이종 데이터에 적용해 보았으며, 이러한 데이터에 있어서 주요 상관관계가 있는 패턴들을 추출한 결과를 제시한다.

• 천문학회보
• /
• 제41권1호
• /
• pp.77.3-77.3
• /
• 2016

In our previous study, we showed that the peculiar globular cluster M22 contains two distinct stellar populations with different physical properties, having different chemical compositions, spatial distributions and kinematics. We proposed that M22 is most likely formed via a merger of two GCs with heterogeneous metallicities in a dwarf galaxy environment and accreted later to our Galaxy. In their recent study, Mucciarelli et al. claimed that M22 is a normal mono-metallic globular cluster without any perceptible metallicity spread among the two groups of stars, which challenges our results and those of others. We devise new strategies for the local thermodynamic equilibrium abundance analysis of red giant branch stars in globuar clusters and show there exists a spread in the iron abundance distribution in M22.

• 대한수의학회지
• /
• 제59권2호
• /
• pp.89-96
• /
• 2019

Enterovirus species G (EV-G) is highly diverse, and is ubiquitous in pig populations, usually without diarrhea. The present study aimed to investigate the presence of novel EV-G recombinants with the torovirus papain-like cysteine protease (PLCP) in Jeju pig herds. EV-G1-PLCP mono-infections were most prevalent in diarrheic weaned piglets. The PLCP genes of the Jeju isolates varied in size and junction sequences, and were greatly heterogeneous, with 77.0-90.7% homology amongst all recombinants. Our results suggest that the exogenous PLCP gene has undergone continuous rapid mutation in the individual EV-G genomes following cross-order recombination, thereby causing clinical disease in swine.

• Molecules and Cells
• /
• 제45권10호
• /
• pp.673-684
• /
• 2022

The past two decades have witnessed an upsurge in the appreciation of adipose tissue (AT) as an immunometabolic hub harbouring heterogeneous cell populations that collectively fine-tune systemic metabolic homeostasis. Technological advancements, especially single-cell transcriptomics, have offered an unprecedented opportunity for dissecting the sophisticated cellular networks and compositional dynamics underpinning AT remodelling. The "re-discovery" of functional brown adipose tissue dissipating heat energy in human adults has aroused tremendous interest in exploiting the mechanisms underpinning the engagement of AT thermogenesis for combating human obesity. In this review, we aim to summarise and evaluate the use of single-cell transcriptomics that contribute to a better appreciation of the cellular plasticity and intercellular crosstalk in thermogenic AT.

• 생명과학회지
• /
• 제16권5호
• /
• pp.812-827
• /
• 2006

멸치의 유전적 집단구조 및 지리적 거리를 조사하기 위하여 한국근해 및 외해역 12개 정점에서 채집된 멸치의 미토콘드리아 DNA control 부위를 대상으로 염기서열을 상호 비교 및 분석했다. 염기서열 분석결과 89개체 중 29 haplotype이 나타났고, 상호 염기치환율은 0-3.5% 차이를 보였다. E9 haplotype이 근해 및 외해역에서 가장 넓게 분포하고 있는 것으로 나타났다 (58.3%). 반면에, E26, E27, E28, E29 haplotype 들은 서남해역 (정점 10)에서만 보였다. PHYLIP 프로그램을 이용한 유전적 관계에서도 두개의 clade로 분리되었다. E26, E27, E28, E29 haplotype을 제외한 나머지 haplotype 들은 상호 잘 유지되는 것으로 나타났다 (bootstrap 75% 이상). 그러나 clade A와 B bootstrap은 매우 약하게 나타났다 (51%). haplotype 간의 상호분석 결과 다양도는 0.75-1.00, 염기다양도는 0.015-0.0244로 보였다.

• 한국산림과학회지
• /
• 제98권1호
• /
• pp.1-7
• /
• 2009

본 연구에서는 눈측백나무(Thuja koraiensis Nakai) 4개 천연집단을 대상으로 84개체를 선발한 뒤 다형성을 보인 29개의 I-SSR amplicons를 이용하여 유전변이를 조사하였다. 6개의 I-SSR primer로 유전다양성을 추정한 결과 평균 유효대립유전자의 수($A_e$)는 1.44개, 이형접합도의 기대치($H_e$)는 0.258, Shannon의 다양성 지수(S.I.)는 0.385로 크게 높지는 않은 것으로 추정되었다. 이를 다양한 표지자를 이용하여 측백나무과(Cupressaceae)에 속하는 지금까지 연구된 수종들과 비교하여보면 국내의 눈측백나무는 유사하거나 다소 높은 유전변이량을 보유하고 있는 것으로 나타났다. 조사된 4개의 집단을 대상으로 AMOVA를 수행한 결과 전체 유전변이 가운데 13%가 집단간 차이로부터 기인하는 것으로 나타났고, 나머지 87%는 집단내 개체간 차이로부터 기인한 것으로 나타났다. 유전적 거리에 의한 UPGMA 유집분석을 실시한 결과 지리적인 경향은 나타나지 않았다. 유전변이량이 가장 높은 장산집단과 유전적 조성에서 가장 이질적인 방태산집단이 보전 가치가 큰 것으로 판단된다.