• Korean Journal of Weed Science
• /
• v.14 no.2
• /
• pp.94-100
• /
• 1994

This experiment was conducted to determine selective mechanism of cyhalofop-butyl ester ((((R-butyl 2-(4-(4-cyano-2-fluorophenoxy) phenoxy) propionate)) between rice and Echinochloa crus-galli. 100ppm of cyhalofop-butyl ester inhibited over 90% of seedling growth of E. crus-galli when applied at 3 leaf stage and complete inhibition was observed at 180ppm applied at the 4 leaf stage, but rice(Chucheongbyeo) was not inhibited by cyhalofop-butyl ester even at 230ppm, regardless of its growth stages(3, 4, 5 and 6 leaf stages). Cyhalofop-butyl ester applied through stem at 10 and 50ppm moved most rapidly to the meristem and resulted in the highest injury on plant height, root length and fresh weight of E. crus-galli. compared with root or leaf application. Seedlings of rice and E. crus-galli at 3 or 4 leaf stage were dipped in 180ppm of cyhalofop-butyl ester solution for 1 minute and aboveground parts of E. crus-galli and rice were removed immediataly after dipping treatment. Regrowth of E. crus-galli was inhibited by the herbicide by 41.7%, but no inhibition was observed in rice. Further, content of chlorophyll reduced to 18.7% of the untreated control, showing appearence of almost being killed, but no effect on chlorophyll content of rice was observed.

• Korean Journal of Weed Science
• /
• v.11 no.3
• /
• pp.178-186
• /
• 1991

This study was conducted to evaluate the major weeds occurred in direct seeded rice and their compition with rice, and to establish the effective weed control method. The important weeds occurring in dry direct seeded rice were Echinochloa crusgalli, Cyperus amuricus, Rorippa islandica, etc. Among them, Echinochloa crusgalli was the most dominant weed species. In view of the Simpson's dominance index, the maximum number of the weeds was observed at 30 days after seeding, showing 0.26, but decreased as the time passed. In other hand, Simpson's dominance index of weed dry weight increased from 0.09 at 15DAS(days after seeding) to 0.28 at 60DAS indicating that particular weed such as barnyardgrass was dominating the fields. The plant height and the tiller number of rice in the dry direct seeded rice were not greatly affected by the time and duration of competition with weeds, but rice yield was greatly influenced by them. The yield reduction was observed when compition between rice and weeds were initiated at 20 to 40 DAS till the harvesting time. The highest weed control efficacy was observed at the treatment of systematic herbicide application such as soil applied Butachlor followed by Mefenacet/Bensulfuron-methyl/Dymron at 30 DAS after seeding, and soil applied Dimepiperate/Bensulfuron-methyl mixture as a preemergence type gave also an excellent control.

• Korean Journal of Environmental Agriculture
• /
• v.14 no.3
• /
• pp.329-337
• /
• 1995

The effect of N-(4-chlorophenyl) maleimide(CPMI), plant growth regulators, and alkylating agents on gluathione(GSH) content and glutathione S-transferase(GST) activity was examined with 3-day-old etiolated sorghum(Sorghum bicolor [L.] Moench) seedlings. The GSH content and GST activity of untreated seedlings were higher in shoots than that in roots. Response of GST activity in coleoptile was significantly greater than in other tissues of sorghum seedling. In CPMI-treated seedlings, GSH content was not significantly different from that in untreated seedlings. CPM treatment resulted in 2.3-fold increase in GST activity measured with metolachlor as substrate in the coleoptile region. In contrast, change in GST activity measured with metolachlor as substrate in the coleoptile region. In contrast, change in GST activity measured with 1-chloro-2, 4-dinitrobenzene did not occur. The increase of GST activity was caused by induction of a GST isozyme, which is substrate-specific to metolachlor. Subsequently, two hypotheses related to metolachlor detoxification were evaluated on the basis of regulation of plant growth regulators and substrate induction of GST activity. In coleoptile, GST activity measured with metolachior was increased to 2.1-and 3.4-fold by both 2, 4-dichlorophenoxyacetic acid(2,4-D) and metolachlor treated at the germination stage of sorghum, respectively. Treatments of 2.4-D and metolachlor also induced isozymes exhibiting the activity toward metolachlor. One of the isozymes was co-eluted with that induced by CPMI. These results indicated that increase in GST activity by CPMI may be partially related to auxin regulation and substrate induction.

• Asian Journal of Turfgrass Science
• /
• v.19 no.2
• /
• pp.65-72
• /
• 2005

Creeping bentgrass (Agrostis palustris Huds.) had been the problematic weed for Kentucky bluegrass (Poa pratensis L.) fairway since it shows light green color all year. Experiment was carried out to determine the best herbicides combination to control creeping bentgrass in Kentucky bluegrass. fairway without injury. To investigate the efficacy of herbicides, five post-emergence herbicides of asulam WG ($87.6\%$), imazaquin SL ($20\%$), fenoxaprop-P-ethyl EC ($7\%$), mecoprop SL ($50\%$), triclopyr-TEA SL ($30\%$) and one pre-emergence herbicide pendimethalin EC ($31.7\%$) treated on 21 Sept. and 10 Nov. 2003. Kentucky bluegrass visual quality evaluated 30 and 50 days after application for phytotoxic effects of the herbicides. As a result, asulam WG (0.2g/$m^{2}$) and imazaquin SL (0.3ml/$m^{2}$) showed approximately $90\%$ of control in creeping bentgrass, but visual quality of Kentucky bluegrass significantly decreased from 20 to 50DAT (day after treatment). However, creeping bentgrass was acceptably controlled(over $80\%$) by fenoxaprop-P-ethyl EC (0.4ml/$m^{2}$)+triclopyr-TEA SL(0.3 ml/$m^{2}$) applied twice on 21 Sept. and 1 Oct. 2003 without serious injury on Kentucky bluegrass. Therefore, it is suggested that an application of fenoxaprop-P-ethyl EC (0.4ml/ $m^{2}$)+triclopyr-TEA SL (0.3 ml/$m^{2}$) may be more effective to control creeping bentgrass in Kentucky bluegrass with the least phytotoxicity by herbicides.

• Korean Journal of Weed Science
• /
• v.10 no.2
• /
• pp.114-121
• /
• 1990

Inactivation in soil absorption, translocation of 2, 4-D by plants vary depending upon soil environments and herbicide formulations. Experiment was conducted in a glasshouse using rectangular pots($1350cm^2$) to evaluate the growth responses of barnyardgrass (Echinochloa crus-galli) and Indian jointvetch (Aesehyrcomene indica) to two formulations of 2, 4-D. The formulations used were 40% 2, 4-D amin salt (2, 4-D/AS) and 19.7% complex of rice husk lignin and 2, 4-D (2, 4-D/LG) which were applied at 200g ai/ha. Soil environments included fertilizer levels, soil pH, organic matter contents, and soil textures, Each treatment was replicated three times. The herbicidal activity of 2.4-D increased and lasted with increased levels of fertilizer. The activity also increased and lasted with low soil pH and decreased content of organic matter. Generally 2, 4-D/LG showed higher and longer herbicidal activity than 2. 4-D/AS for both test plants under all conditions applied. However, the herbicidal activity was influenced by the formulations more than by soil textures. It was thought that 2, 4-D/AS was released in a short time and inactivated readily while 2, 4-D/LG was slowly released and gave an opportunity of absorption by plants for a long period.

• Korean Journal of Weed Science
• /
• v.30 no.3
• /
• pp.272-281
• /
• 2010

The objective of this research was to find out the weed management techniques in environment-friendly rice paddy fields through the study on herbicidal effects and problems of various organic materials. This experiment was conducted under different conditions of weed species and weed densities in environment-friendly rice paddy fields. There was no difference in weedy efficacy on golden apple snail (GAS), paper mulching (PM), and machine weeding (MW) between low and high weed densities. However, the effect of weed control in rice bran (RB) and effective microorganism (EM) + molasses was higher in high weed density than in low weed density. In general, the effect of weed control as affected by various organic materials was in the order of GAS (97-100%) > PM (93-98%) > RB (15-80%) > EM (7-31%). GAS provided excellent control of all weed species tested except for Persicaria hydropiper. PM gave acceptable control of the weed species except for Echinochloa crus-galli, Ludwigia prostrata, and Eleocharis kuroguwai. However, MW gave fair control (70% biomass reduction) of all weed species tested. BR followed by MW or EM followed by MW treatments had similar effect on weed control compared to each treatment alone. However, BR followed by GAS or EM followed by GAS provided 100% control of weed species tested. The level of rice foliar injury caused by various organic materials was in the order of GAS and MW (10-20%) > RB (10-15%) > PM and EM (5-7%). Typical symptoms of organic materials are wilting, inhibition of growth, missing hill, and reduction of tiller. Cost for weed control of GAS, RB, EM, and PM were 2.1, 3.1, 2.3, and 13.2 times higher than that of the herbicide. These data indicate that GAS was the best method for weed management in environmentfriendly rice paddy fields. Further study is required to elucidate the mechanisms underlying the rice injury as affected by GAS.

• Journal of Plant Biotechnology
• /
• v.30 no.4
• /
• pp.405-410
• /
• 2003

Environmental stress is the major limiting factor in plant productivity. In order to evaluate the stress tolerance of potato plants, leaf discs of two potato cultivars, Atlantic and Superior, were subjected to various stress conditions of high temperature, methyl viologen, H2O2, or $H_2O$$_2$. When potato leaf discs were exposed to high temperature at 37$^{\circ}C$ for 84 hr, Atlantic plants, a cultivar with high sensitivity to heat stress, showed about 20％ higher membrane damage than Superior plants. When exposed to 2$\mu$M methyl violgen (MV), a superoxide generating non-selective herbicide, for 36 hr, Atlantic plants also showed about 38％ higher membrane damage than Superior plants, and were more susceptible up to 10$\mu$M MV concentration tested. On treatment with 0.75M NaCl, Atlantic plants also had about 45％ less chlorophyll contents in leaf discs than Superior plants. There was, however, no difference in chlorophyll content of two cultivars at higher NaCl concentrations. The effect of $H_2O$$_2$ on the two cultivars was mixed. At low $H_2O$$_2$ concentration (25 mM) , Superior plants were more susceptible to $H_2O$$_2$stress after 36 hr. However, at high $H_2O$$_2$ concentration (100 mM), Atlantic plants exhibited higher susceptibility after 36 hr. The results indicate that in vitro leaf discs reflecting the whole plants in this study will be useful for selection and characterization of elite transgenic potato plants with enhanced tolerance to environmental stress.

• Korean Journal of Organic Agriculture
• /
• v.19 no.1
• /
• pp.65-82
• /
• 2011

In order to evaluate the weed suppressing effect of Solanum viarum Dunal. In this study species diversity in patch of S. viarum and allelopathic effects of the aqueous extracts on S. viarum were investigated. Number of species and species diversity in site close to patch of S. viarum were decreased gradually 1site ($7.7{\pm}2.0,\;1.5{\pm}0.2$), 2site ($5.3{\pm}1.2,\;1.2{\pm}0.2$) and 3site ($4.0{\pm}1.7,\;0.9{\pm}0.1$). And total phenolic compounds of soil in survey area were increased gradually site1 ($0.16{\pm}0.01mg\;g^{-1}$), site2 ($0.17{\pm}0.01mg\;g^{-1}$) and site3 ($0.22{\pm}0.02mg\;g^{-1}$). So the number of species and species diversity (r=-0.692, P<0.05) were negatively correlated with increased total phenolic compounds of soil in the survey area. The relative germination ratio, the mean germination time, the relative elongation ratio, the fresh weight and the dry weight of receptor plants are generally getting decreased while the concentration of the aqueous extracts from S. viarum escalate. But every ratio was various depending on the growing regions, the kind of receptor plants and the treatment of the aqueous extracts. Especially, the radicle by injection of the aqueous extracts concentration of S. viarum was influenced more than the shoot on the same condition. The total phenolic compounds on region of S. viarum was gradually increased in stems (fresh $0.56{\pm}0.02mg\;g^{-1}$, dry $1.58{\pm}0.08mg\;g^{-1}$), roots (fresh $1.77{\pm}0.07mg\;g^{-1}$, dry $2.64{\pm}0.06mg\;g^{-1}$), leaves (fresh $6.01{\pm}0.14mg\;g^{-1}$, dry $7.04{\pm}0.29mg\;g^{-1}$), seeds (fresh $6.21{\pm}0.17mg\;g^{-1}$, dry $9.08{\pm}0.73mg\;g^{-1}$) in order. On the contrary, the negative correlation on germination and growth of receptor plants was shown by total phenolic compounds on the each parts of S. viarum. We think that the aqueous extracts of S. viarum showed allelopathic effects on other plants. Therefore, S. viarum holds the higher competitiveness in plant community in Jeju Island and makes possibility of application as natural herbicide.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.41 no.5
• /
• pp.619-624
• /
• 1996

An experiment was carried out to find out the effects of water foxtail on weed control and rice yield as well as on the reduction of nitrogen application in no tillage transplanted paddy field. Paddy field was dominated by water foxtail whose soil covering degree was adjusted from 8 to 6 by treatment of paraquat (70 l /10a of solution diluted by 1, 000 times). Thirty five-day old seedling was mechanically transplanted and reduced nitrogen, 80% of conventional application was applied at the paddy field. The higher failure in seedling stand was observed at higher degree of soil covering by water foxtail. The failure of seedling stand with covering degree of 8(no paraquat) was 37.4%, while that of covering degree of 6(paraquat-treated) was 12.3%. However, the seedling stand failure was completely recovered at covering degree of six at two weeks after transplanting. The mechanical transplanting made water foxtail in the paddy field fall on the ground whose panicle part was recovered from falling sometimes after transplanting and whole plant died with slow scenesence untill the late of June. But the water foxtail affected by paraquat produced the new panicle from uppernode of stem with dead leaves at early of June and it become die slowly untill the early of July. Though several rice field weeds were observed in the order of Echinochloa crusgallis, Cyperus serotinus, Lemma paucicostata during the active tillering stage of rice, the value of weed control due to the dominance of water foxtail was 77~78%. The death and subsequent decay of water foxtail during the active tillering stage of rice induced the soil reduction which again defect the growth of rice root. The more vigerous rice growth was observed in the plot dominated with water foxtails, than control plot. The yields of rice in the water foxtail (degree 6) plot was 629kg per l0a as brown rice, the same as that of control plot. In conclusion, the no-tillage transplanting with control of covering degree of water foxtail (to degree 6) was effective in reduction of input cost such as herbicide and nitrogen fertilizer, as well as weed control without reduction of rice yield.

• Tuberculosis and Respiratory Diseases
• /
• v.41 no.3
• /
• pp.222-230
• /
• 1994

Background: Paraquat, a widely used herbicide, is extremely toxic, causing multiple organ failure in humans. Paraquat especially leads to irreversible progressive pulmonary fibrosis, which is related to oxygen free radicals. However, its biochemical mechanism is not clear. Natural mechanisms that prevent damage from oxygen free radicals include changes in glutathione level, G6PDH, superoxide dismutase(SOD), catalase, and glutathione peroxidase. The authors think catalase is closely related to paraquat toxicity in the lungs Method: The effects of 3-amino-1,2,4-triazole(aminotriazole), a catalase inhibitor, on mice administered with paraquat were investigated. We studied the effects of aminotriazole on the survival of mice administered with paraquat, by comparing life spans between the group to which paraquat had been administered and the group to which a combination of paraquat and aminotriazole had been administered. We measured glutathion level, glucose 6-phosphate dehydrogenase(G6PDH), superoxide dismutase(SOD), catalase, and glutathione peroxidase(GPx) in the lung tissue of 4 groups of mice: the control group, group A(aminotriazole injected), group B(paraquat administered), group C(paraquat and aminotriazole administered). Results: The mortality of mice administered with paraquat which were treated with aminotriazole was significantly increased compared with those of mice not treated with aminotriazole. Glutathione level in group B was decreased by 20%, a significant decrease compared with the control group. However, this level was not changed by the administration of aminotriazole(group C). The activity of G6PDH in all groups was not significantly changed compared with the control group. The activities of SOD, catalase, and glutathione peroxidase(GPx) in the lung tissue were significantly decreased by paraquat administration(group B); catalase showed the largest decrease. Catalase and GPX were significantly decreased by aminotriazole treatment in mice administered with paraquat but change in SOD activity was not significant(group C). Conclusion: Decrease in catalase activity by paraquat suggests that paraquat toxicity in the lungs is closely related to catalase activity. Paraquat toxicity in mice is enhanced by aminotriazole administration, and its result is related to the decrease of catalase activity rather than glutathione level in the lungs. Production of hydroxyl radicals, the most reactive oxygen metabolite, is accelerated due to increased hydrogen peroxide by catalase inhibition and the lung damage probably results from nonspecific tissue injury of hydroxyl radicals.