• Korean Journal of Weed Science
• /
• v.6 no.2
• /
• pp.191-200
• /
• 1986

The study was conducted to screen paraquat-tolerant plant species among crops and weeds, using the response of plant like leaf disc discoloration, visual injury and dry weight in the presence of paraquat. Mechanism of paraquat-tolerance was investigated in strains of soybean through evaluating activities of superoxide dismutase and peroxidase and the multiplication of callus derived from soybean cotyledon. In crops, Kwanggyo has been selected as a paraquat-tolerant variety among soybean cultivars tested, and Hood as a susceptible one. In weeds, Polygonum aviculare, Chenopodium album and Pinellia ternata were evaluated as the paraquat resistant species, providing the possibility for the donor plant species for paraquat resistance. Activity of superoxide dismutase known to detoxify paraquat was markedly greater in Kwanggyo, a paraquat-tolerant cultivar than in Hood, a susceptible one. In addition, the similar response like superoxide dismutase was observed in peroxidese activity. The greater inhibition of callus multiplication was determined in Hood, a susceptible one than a tolerant one, Kwnggyo. Based on all the informations, it is strongly proposed that paraquat tolerance in soybean is due to destruction of $O_2^-$ by elevated concentration of superoxide dismutase in the tolerant cultivar.

• Korean Journal of Weed Science
• /
• v.16 no.3
• /
• pp.237-244
• /
• 1996

Propanil [N-(3,4-dichlorophenyl) propanamide] which was applied at 4,200g ai/hapostemergence 7 days after seeding or transplanting, completely reduced the growth of shoot and root of barnyardgrass at 100% under dry condition while plant height, root length and shoot fresh weight of barnyardgrass at 63, 40 and 78%, respectively under water condition. On the other hand, the herbicide did not affect the growth of shoot and root of rice grown under water condition and transplanting condition, but reduced the plant height, root length and shoot fresh weight of broadcast rice on soil at 24, 18 and 28%, respectively, under dry condition. Microscopically, the epidermal cells of treated-barnyardgrasses under both conditions were severely constricted, chloroplasts in the cells of vascular bunble sheath were partially lacked, and mesophyll cells were often ruptured, whereas those of treated-rice were not affected. Histological observations showed that propanil reduced the thickness of leaf blade of barnyardgrass under both conditions at 36-48% due to mainly reduction and constriction of mesophyll cell, while it did not affect or even increased the thickness of leaves of rice under all conditions compared to control. These results indicate that broadcast rice on soil were more injured than drilled rice in soil under dry condition, however, in the other tested conditions ricer were not affected.

• Journal of Life Science
• /
• v.31 no.3
• /
• pp.280-286
• /
• 2021

Oryzalin is a herbicide that disrupts the arrangement of microtubules by binding to tubulin, thereby blocking the anisotropic growth of plant cells. Microtubules and microfilaments are cytoskeleton components that have been implicated in plant growth through their influence on the formation of cell walls. Microtubules also play roles in the sedimentation of amyloplasts in the root tip columella cells; this sedimentation is related to gravity sensing and results in downward root growth in the soil for absorption of water and minerals. However, the orientation of microtubules changes depending on the level of ethylene in plant cells. A recent study reported that oryzalin stimulated ethylene production via 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase and caused a concentration-dependent inhibition of root growth and gravitropic responses. The aim of the present study was to investigate the possibility that oryzalin-induced inhibition might be recovered by the application of inhibitors of ethylene production, such as 10-4 M cobalt ions and 10-8 M aminoethoxyvinylglycine (AVG). The inhibition of root growth and gravitropic response was overcome by 10-20% by an 8 hr treatment with cobalt ions or AVG. These results suggest that ethylene levels could regulate root growth and gravitropic responses in Arabidopsis.

• Korean Journal of Weed Science
• /
• v.30 no.3
• /
• pp.300-307
• /
• 2010

Greenhouse experiment was conducted to evaluate tolerance of six rice cultivars, three Indica${\times}$Japonica rice (long grain, cv. Dasanbyeo, Segejinmi and Hanareumbyeo) and three Japonica rice (short grain, Nampyung, Ilpumbyeo and Junamjosaeng) cultivars, to mesotrione+pretilachlor (MP) and bensulfuron-methyl+mesotrione+pretilachlor+pyriftalid (BMPP) in transplanting rice. Two herbicides were applied at 90 g and 180 g a.i. $ha^{-1}$ to three to four leaf stage rice at 5 and 15 days after transplanting, respectively. Related study was also conducted to compare $GR_{50}$ for Indica${\times}$Japonica and Japonica rice cultivars at different MP rates. Response to two herbicides varied with respect to rice cultivars and herbicide rates. All Indica${\times}$Japonica rice cultivars exhibited susceptible to both MP and BMPP as reflected by increased visual injury, shorter plant height and higher plant dry matter reduction when compared with nontreated rice cutlivars. The degree of foliar chlorosis by MP at 90 g a.i. $ha^{-1}$ was 5~6 at 7 days after treatment(DAT) but it was decreased to 3~6 at 14DAT. The degree of leaf chlorosis treated with 180 g a.i. $ha^{-1}$ MP was 6~7 at 7DAT and it was also decreased to 3~8 at 14 DAT. The plant hight of Indica${\times}$Japonica rices was inhibited by 18~43% at application of 90 g a.i. $ha^{-1}$ MP and 30~50% at 180 g a.i. $ha^{-1}$ MP. The dry matter reduction was greater than that of plant height inhibition, showing 46~73% at 90 g a.i. $ha^{-1}$ MP and 65~82% at 180 g a.i. $ha^{-1}$ MP. Similar leaf chlorosis injury and growth inhibition of rice cultivars was observed in the BMPP treatment. The injury and growth inhibition by MP and BMPP increased with increase in herbicide rate from 90 g a.i. $ha^{-1}$ to 180 g a.i. $ha^{-1}$. However, most of the Japonica cultivars are tolerant to MP and BMPP at both rates. There was no visible leaf chlorosis but plant height and dry matter production were slightly reduced at 180 g a.i. $ha^{-1}$. Based on $GR_{50}$ value determined in reduction of shoot dry weight for MP, the Indica${\times}$Japonica rice showed 12.9 fold lower compared with the Japonica rice. The result indicates that rice cultivars vary in tolerance to herbicides of MP and BMPP and Indica${\times}$Japonica rices were more susceptible than the Japonica rices to the MP and BMPP.

• Korean Journal of Weed Science
• /
• v.12 no.2
• /
• pp.132-143
• /
• 1992

The study was conducted to find out synergistic effects by interaction on the basis on anatomical changes between paddy rice and weed species by mixture use of oxyfluorfen and bensulfuron at 0, $10^{-6}$ and $10^{-4}$M, respectively. Tissues were sampled at 72 hrs after soaking treatment and prepared for light microscopic examination and sectioned longitudinally and transversely stem into $8{\mu}m$ thick. As the results of microscopic examination, the major response of treated plant involved rupture and constrict in mesophyll cells and epidermal cells, and shrinks in bundle sheath cells by oxyfluorfen and disorganization and swelling in meristems by bensulfuron. Anatomical changes in rice was the least affected by even high concentration and two herbicide mixtures, whereas Echinochloa crus-galli was severely disorganized in meristem regions and ruptured in epidermal cells by mixture use. Monochoria vaginalis was completely ruptured by any treatment concentrations and mixtures. Scirpus juncoides was considerably tolerant to both herbicides and their mixture and was similar to those that occur in rice. Perennial weed, Sagittaria pigmaea was observed vacuolation, non-nucleation of cell, and irregular cell layering but in mixture injury was slight. Cyperus serotinus was severely shrunk and ruptured by mixture treatment. Eventually anatomical variations in all weed species was shown synergistic effect by use of herbicide mixtures.

• Korean Journal of Weed Science
• /
• v.15 no.1
• /
• pp.30-38
• /
• 1995

Soil-applied pre-emergence herbicide, pyrazolate(4-(2, 4-dichlorobenzoyl)-1, 3-dimethyl pyrazol-5-yl-p-toluene sulphonate) induced, twist effect of shoots of barnyardgrass under dry conditions, and etiolated leaf and stem of that under water condition. Plant height and root length of rice broadcast on soil surface were similar to the untreated control, but plant height of rice drilled in soil was more inhibited than root length as compared with the untreated control, while development of barnyardgrass seedling was severely inhibited at 20 days after application. The inhibition rate was much higher under water condition than under dry condition, but difference in rice and barnyardgrass did not abserve. However, growth of transplanted rice shown to increase to the untreated control. Shoot and root fresh weight of rice broadcast on soil surface was increase as compared with the untreated control, and that of rice drilled in soil was not affected whereas that of barnyardgrass was severely inhibited by 42% and 41%, respectively. Under dry condition at 20 days after pyrazolate application while root growth of rice broadcast on soil surface under water condition was deadly inhibited and development of barnyardgrass was almost completely inhibited. On the other hand, microscopic studies showed that constriction of mesophyll cell by destruction of chloroplast of barnyardgrass were occurred only under dry condition, whereas damage of rice and barnyardgrass under water and transplanting condition were not observed. Anatomical change in the meristernatic region of rice and barnyardgrass was not occurred, and similar to intact plant regardless of cropping patterns.

• Korean Journal of Weed Science
• /
• v.16 no.3
• /
• pp.187-193
• /
• 1996

To supply seeds with a good quality as plant materials for herbicide screening and to know whether the germination characteristics could be associated with a differential response to herbicides, germination characteristics and differential responses to herbicides were investigated with 3 species of a genus Setaria ; Yellow foxtail(Setaria glauca P. Beauv, SETGL), Giant foxtail(Setaria faberi Hetrm, SETFA), and Green foxtail(Setaria viridis P. Beauv, SETVI). Degree of dormancy was high in the order of SETGL, SETVI and SETFA. The dormancy of SETGL seed was relatively well removed by room temperature and drying storage, but SETFA and SETVI by low temperature and wetting storage(stratification). For breaking dormancy of SETGL, SETVI and SETFA, it was necessary for being kept under the above storage conditions for at least 2, 4 and 4-5 months, respectively. When the dormancy-breaked seeds were transfered to low temperature($4^{\circ}C$) and drying condition, SETGL showed germination rate of 96% even after 2 month storage. However, SETVI and SETFA showed a decreased germination of 54% and 69%, respectively, with a decreased velocity of germination, indicating that secondary dormancy might be induced. On the other hand, a significant change in germination rate was not observed as the seeds were transfered to room temperature($25^{\circ}C$) and drying condition. The germinability of SETGL seed began to decline from 6th year after storage in room temperature and drying condition. All of 3 species showed relatively high germination rate at alternating temperature of $30^{\circ}C$/$20^{\circ}C$(14hr/10hr) and their germination were not increased by light. All of 3 species exhibited similar responses to cycloxydim, sethoxydim and primisulfuron in greenhouse experiment. In contrast, SETVI and SETFA were relatively susceptible to fenoxapropethyl, SETFA to fluazifop-butyl, SETGL and SETFA to clorimuron-ethyl, and SETGL to EK-2612. The difference in herbicidal response among 3 species was the highest in the treatment of EK-2612. These results suggest that there is no a consistent tendency in responses of 3 species to herbicides which have the same target site. And the relationship between germination characteristics and differential responses to herbicides was not found.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.36 no.2
• /
• pp.107-111
• /
• 1991

This study was conducted to define the effect of 2, 4-D foliar application on the plant growth. photosynthesis ability and ethylene gas production of the ginseng plant. Neither abnomal foliar changes occured nor any inhibition in the leaf and stem growth was resulted for the plants treated with 2,4-D concentrated three times of the recommended dosage. The angle of petiole to the stem became wider by 2, 4-D foliar application. The higher concentration treatment of 2,4-D made the petiole angle significantly wider. Foliar application of the herbicide 2,4-D inhibited photosynthesis of the ginseng leaf. The inhibition rate of photosynthesis was significantly increased with the application concentration of 2, 4-D. Inhibition in photosyn-thesis ability by 2,4-D application with doubled concentration was recovered in three days after treatment. When 2,4-D was treated with a concentration tripled the recommended dosage, 12 days were needed to recover the photosynthesis ability of the ginseng leaf. Ethylene gas was not detected from the ginseng plants treated with 2 times concentrated 2, 4-D. However, the ginseng plants produced 0.03-0.04 ppm ethylene gas when the application rate was increased 3 times. The amount of ethylene gas produced by ginseng plant treated with 3 times concentrated 2, 4-D was only 1/20 compared with the amount produced by the soybean plant treated with the recommended dosage of 2, 4-D.

• Korean Journal of Weed Science
• /
• v.2 no.1
• /
• pp.13-19
• /
• 1982

The effect of metolachlor [2-chloro-N-(2-ethyl 1-6-methylphenyl)-N-(2-methoxy-l-methylethyl)-acetamide] on starch, sugar and protein content in relation with ${\alpha}$-amylase activity, and its interaction with GA in the germinating stage of rice were determined. The distinctive phytotoxic symptom was the failure of the primary leaf of rice to break the coleoptile. An inhibitory effect of metolachlor was nullified by the external application of GA $10^{-3}$M. A significantly slower starch degradation accompanying the lower content of sugars was observed in the gain of the metolachlor treated seedlings. Further, the total ${\alpha}$-amylase activity was significantly lower in the grain of rice seedlings treated with metolachlor than that of the untreated one, and the lower total ${\alpha}$-amylase activity could be due to an inhibition of ${\alpha}$-amylase formation.

• Korean Journal of Weed Science
• /
• v.17 no.2
• /
• pp.169-175
• /
• 1997

The effect of different cultural patterns on the growth and herbicidal response of rice(Oryza sativa L.) and barnyardgrass(Echinochloa crus-galli var. oryzicola) was studied tinder direct-seeded rice and early rice seedling conditions. In dry-seeded rice condition, the growth of rice and barnyardgrass was the greatest in 3cm-seeding depth and 40% soil moisture, respectively. At 10 days after herbicide application, plant height of rice was severely inhibited in 1cm-seeding depth and 50% soil moisture, and the degree of inhibition was severer at application of pendi+molinate EC as compared to that of butachlor EC. On the other hand, plant heigth of barnyardgrass by treatment of herbicides was severely reduced regardless of seeding depth and soil moisture. The lengths of barnyardgrass mesocotyl and rice coleoptile were increased with increasing seeding depth and were severely inhibited by applied herbicides in 1cm-seeding depth under dry-seeded rice condition. The dry weight of rice at different leaching times after herbicides application showed slightly decreasing trend as the leaching time was delayed, but the growth of barnyardgrass was very severely inhibited regardless of the leaching time. In addition, the phytotoxicity of rice showed decreasing trend with the increase in transplanting depth and delay of application time under early rice seedling condition.