• Weed & Turfgrass Science
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• v.5 no.4
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• pp.187-190
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• 2016

This study was conducted to determine phytotoxicity of domestic rice varieties and characteristic of sensitive gene involved in herbicide reaction of 4-HPPD (4-hydroxy phenylpyruvate dioxygenase) inhibiting herbicides. The five rice varieties were grown for 2 to 3 leaf stage on seedling trays and then transplanted into plastic pot: 4-Japonica type (Sangjubyeo, Sambaekbyeo, Sanduljinmi and Kumyoung) varieties and 1-Indica type variety (IR8). We trialled standard (14 g a.i. $10a^{-1}$) and double fold (28 g a.i. $10a^{-1}$) dose of benzobicyclon treatment at 10 days after transplanting in order to investigate phytoxicity. The Japonica-type Sangjubyeo showed no rice injury but Indica-type IR8 show 4-5 (standard) and 5-6 (double fold) phytoxicity levels. In spite of Japonica-type, Sanduljinmi and kumyoung showed 3-4 and 4-5 levels. Target resistant gene, Hydroxyphenylpyruvate dioxygenase inhibitor sensitive gene No.1 (HIS1) have been isolated from five domestic rice cultivars (Sangjubyeo, Sambaekbyeo, Sanduljinmi, Kumyoung and IR8). Results of the sequence through PCR, all five tested rice cultivars had HIS1 gene regardless to rice cultivars. And the difference between rice varieties from sequence of HIS1 were identified some variation in genes.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.86-108
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• 2017

The nationwide weed survey was conducted in arable land, paddy field, upland, orchard and pasture, over whole country of Korea during 3 years from 2013 to 2015. Also, these survey were conducted in order to determine a change of weed community and to identify a major dominant weed species, and problem weeds. Weeds of arable land were composed of 619 species belonging to 81 families. Weeds occurred in paddy field were 90 species belonging to 28 families, upland weeds were 375 species of 50 families, orchard weeds were 492 species of 63 families and pasture weeds 275 species of 52 families. Among total 81 families, the Asteraceae (15.5%) was the biggest family, followed by Poaceae (13.1%), Fabaceae (5.5%), Cyperaceae (5.2%), Polygonaceae (6.3%), respectively. And these weed species were composed of annual weeds of 209 species (33.8%), winter annual of 102 species (16.4%) and perennial weeds of 308 species (49.8%). Exotic weeds naturalized in Korea were investigated as 166 species. Weed flora in Korean cultivation area have been increased as much as 13 families and 186 species for 10 years compared to 2000~2004 weed survey result of 68 families and 433 species. The increment could be resulted from the change of weed population such as increase of the herbicide-resistant and the difference of investigation method.

• Korean Journal of Weed Science
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• v.32 no.3
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• pp.240-255
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• 2012

The objectives of this study were to investigate the levels of phytotoxicity of rice varieties to HPPD (4-hydroxy phenylpyruvate dioxygenase)-inhibiting herbicides known for their efficiency to control the sulfonylureas-resistant weed species：mestrione, benzobicyclone, and tefuryltrione. The twenty-six rice varieties (8-Japonica ${\times}$ Indica-type varieties and 18-Japonica-type varieties) were grown for 25 days on seedling trays and then transplanted to paddy rice fields followed by herbicide treatment i.e. standard and double doses of there respective herbicides at 5, 10, and 15 days after transplanting. Although mestrione, benzobicyclone and tefuryltrione are all HPPD-inhibiting herbicides, the phytotoxicity symptoms of the different rice varieties based on the timing of application and doses of the herbicides were significantly different. The Japonica ${\times}$ Indica-type varieties showed much more phytotoxicity symptoms than Japonica-type varieties in all applied herbicides. Increasing herbicidal doses of mesotrione, and an earlier application of and increasing herbicidal doses of benzobicyclon caused severe phytotoxicity symptoms. On the other hand, phytotoxicity due to tefuryltrione did not exhibit significant differences between rice varieties in either the timing of application or dose of the herbicide. Regardless of timing of application and dose of the herbicides, Hangangchalbyeo-1, Hyangmibyeo-1 and high-yield rice varieties such as Namcheonbyeo, Dasanbyeo, Areumbyeo, and Hanareumbyeo, which belong to the Japonica ${\times}$ Indica-type varieties, showed 5 to 8 levels of phytotoxicity symptoms including albinism, browning, detached leaf, and necrosis to mesotrione and benzobicyclon whereas only 1 to 3 levels of phytotoxicity symptoms (chlorosis, albinism, and browning) were seen with to tefuryltrione application. The Japonica-type varieties exhibited only slight phytotoxicity symptoms (1~2 levels) in conformity with the timing of application and doses of the herbicides. However, there were significant differences among the Japonica-type rice varieties, depending on the type of herbicide. Thirteen-Japonica type rice varieties were sensitive to benzobicyclone while 4-Japonica-type and 7-Japonica-type varieties showed phytotoxicity symptoms such as chlorosis and albinism with mestrione and tefuryltrione application, respectively. Therefore, we suggest that the combined-type herbicides including mestrione, benzobicyclone and tefuryltrione should be rejected in paddy fields where rice is grown for either human consumption (functional or processed rice) or livestock feed because of severe phytotoxicity symptoms on the various rice varieties seen regardless of the timing of application and doses of the herbicides.

• Korean Journal of Weed Science
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• v.2 no.2
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• pp.152-159
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• 1982

To investigate the effect of isoproturon (N,N-dimethyl-N-4-isopropyl phenyl urea) on weed-killing, crop injury and yield of barley, several experiments were conducted by application time, dosage, cultivars and soil texture. For the effective control of weeds the optimum application time was foliage application after winter. Alopecurus aequalis SOBOL was effectively controlled when isoproturon should be applied at three to four leaf-stage and most annual broad-leave weeds emerged through winter and spring could be controlled when applied even at five to six leaf-stage. But among the broad-leave weeds Vicia amoena Fisch. was resistant to isoproturn. The optimum application rate of isoproturon was 240g-300g/10a (prod.). Among 11 cultivars of barley and wheat, phytotoxicity of Olmil, Jokwang and Rye was slighter than that of the other cultivars when isoproturon was treated by foliage application after winter. At the time of foliage application after winter, the variation of phytotoxicity and effectiveness was a little despite the difference of soil texture and the grain yield of barley was higher in the plots treated 240-300g/10a than in other treated plots.

• Korean Journal of Weed Science
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• v.30 no.3
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• pp.291-299
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• 2010

Cyhalofop-butyl and penoxsulam, known for respective acetyl CoA carboxylase (ACCase) and acetolactate synthase (ALS) inhibitor, have been widely using as a post-emergence foliar application for many years in wet-seeded rice field in Korea. Since 2009, control of Echinochloa oryzoides with cyhalofop-butyl and penoxsulam was no longer satisfactory. Greenhouse and experiment in rice field were conducted to confirm E. oryzoides resistance to ACCase and ALS inhibitors and to compare herbicide treatments for control of the resistant E. oryzoides to ACCase and ALS inhibitors. Three resistant E. oryzoides to ACCase and ALS inhibitors accessions were tested for levels of resistance to cyhalofop-butyl and penoxsulam, base on survival rate. Iksan and Kimje accessions were not any affected to the survival by treatment with recommended doses of two herbicides tested. Buan accession displayed an intermediate response of 62 and 72% survival at recommended doses of cyhalofop-butyl and penoxsulam, respectively. Survival rates to herbicides mixed with ACCase and ALS inhibitors at 3.5 leaf stage of E. oryzoides was over 80%. Benzobicyclon+fentrazamide+bensulfuron SC, benzobicyclon+mefenacet+bensulfuron SC, benzobicyclone +cafenstrole+pyrazosulfuron-ethyl GR controlled effectively by 2 leaf stage of resistant E. oryzoides to ACCase and ALS inhibitors. In the field experiment, single treatment of benzobicyclon+ fentrazamide+bensulfuron SC and benzobicyclon+mefenacet+bensulfuron SC failed to control E. oryzoides, but squential treatment of benzobicyclon+thiobencarb SE and benzobicyclon+ mefenacet+ bensulfuron SC controlled effectively it in rice infant seedling culture with machine. Our results suggest that resistant E. oryzoides to ACCase and ALS inhibitors had not developed multiple resistance to herbicides with different modes of action. In particular, cafenstrole, fentrazamide, mefenacet were effective control measures.

• Korean Journal of Weed Science
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• v.32 no.1
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• pp.35-43
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• 2012

The objective of this research was to confirm the difference in physiological responses to environmental stresses such as chilling, high temperature, NaCl, and chemical stress (paraquat) in Protox inhibitor resistant-transgenic rice (MX, PX, and AP37) and its non-transgenic counterpart (WT). Transgenic and non-transgenic rice plants were exposed to a chilling temperature of $5^{\circ}C$ for 1 day or a high temperature of $45^{\circ}C$ for 4 days and allowed to recover at $25^{\circ}C$ for 6 days after the chilling treatment or 8 days after the high temperature treatment. Leaf injury, shoot fresh weight, porphyrin biosynthesis substances, and chlorophyll content were investigated in transgenic and non-transgenic rice at 6 days after 0.5% and 1% NaCl treatments or at 5 days after 0~300 ${\mu}M$ paraquat treatments. No significant difference in leaf injury and shoot fresh weight were observed between transgenic and non-transgenic rice during chilling and recovery. Plant height and shoot fresh weight were also similar between transgenic and non-transgenic rice during the high temperature and recovery period (0~5 days). However, plant height and shoot fresh weight in transgenic rice line MX and PX were lower than in non-transgenic rice at 6 days for recovery. Leaf injury, chlorophyll, and Mg-Proto IX ME contents had no significant difference between transgenic rice and non-transgenic rice after NaCl treatment, but Proto IX content for AP37 and shoot fresh weight for PX and AP37 in 0.5% NaCl treatment were significantly reduced compared with non-transgenic rice. There was no difference in leaf injury and shoot fresh weight when comparing transgenic rice and non-transgenic rice after paraquat treatment. Although transgenic rice and non-transgenic rice showed a little difference at a particular measurement period in certain environmental stresses, there was generally no difference in physiological responses between transgenic rice and non-transgenic rice.

• Korean Journal of Weed Science
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• v.18 no.3
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• pp.205-213
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• 1998

This experiment was carried out to produce herbicide resistant potatoes hawing only chimeric phosphinothricin acetyltransferase (PAT) genes without using antibiotic selectable marker. The pDY502 vector having only PAT gene was reconstructed for transformation of potato. The reconstructed vector was introduced to Agrobacterium tumefaciens MP90 disarmed, and they were used for potato transformation. Hormonal requirement for plant regeneration from leaves and stem explants of potato was investigated. From this experiment, MS medium treated with IBA 0.1 mg/L + BA 0.5 mg/L was the best for potato regeneration, and the ratio of shoot regeneration was 54% for leaf and 46% for stem in that condition. For transformation, explants of potato leaves and stems were cocultured with A. tumefaciens MP90 containing reconstructed vector harvoring only PAT gene. When the potato explants were placed on various concentrations of bialaphos and all the potato explants were dead on medium with over 5.0mg/L bialaphos. By this selection methods, the explants cocultured with Agrobacterium produced the putative transgenic shoots on medium with 5mg/L bialaphos treatment after 3-4 weeks. Second selection was performed by transferring the shoot tips of putative transgenic to medium containing 20mg/L of bialaphos. The shoot tips grew well on the second selection medium, indicating the production of successful transgenic plants. But normal shoots were dead in same cytotoxic medium. Incorporation of the PAT gene into transgenic potatos were confirmed by PCR analysis of DNA and Southern hybridization. These results show that the PAT gene can serve as a selectable marker and herbicide resistant genes for transformation of potato.

• Korean Journal of Weed Science
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• v.30 no.4
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• pp.380-389
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• 2010

Transgenic herbicide-resistant sweet potato plants [Ipomoea batatas (L.) Lam.] produced through a biolistic transformation were used in this study. The objective of this research was to find out a rapid and reliable assay method for confirming glufosinate-ammonium resistance. The techniques tested include whole-plant bioassay, one leaf bioassay, and leaf disk bioassay. Parameters investigated in this study were leaf injury and ammonium accumulation at 1 and 5 days after treatment of glufosinate-ammonium. In the leaf disk bioassay, leaf injury of the transgenic line 7171 was 1.9-fold less affected by glufosinate-ammonium than the wild type. The leaf injury of 7171 in one leaf and whole-plant bioassays was 59- and 92-fold less affected by glufosinate-ammonium, respectively, compared with that of the wild type. Leaf disk, one leaf, and whole-plant bioassays showed that ammonium accumulation of the 7171 was 2 to 20-, 4 to 43-, and 6 to 115-fold less affected by 0.5-5 mM glufosinate-ammonium than that of the wild type. All three bioassays successfully distinguished the resistance from the transgenic lines, but one leaf bioassay is the simplest and quickest. Leaf injury and ammonium accumulation were the same in leaves 1, 3, 5, 7, and 10 of 3 mM glufosinate-ammonium treated plants or nontreated plants. The one leaf bioassay was chosen as the standard procedure for future confirmation of resistance in transgenic sweet potato because it is a rapid and reliable assay.

• Korean Journal of Weed Science
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• v.18 no.3
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• pp.237-245
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• 1998

The transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants containing Bacillus subtilis protoporphyrinogen oxidase gene with cauliflower mosaic virus 35S promoter have recently been generated by using Agrobacterium-mediated gene transformation. The nontransgenic and the transgenic tobacco plants were compared with respect to responses to diphenyl ether herbicide oxyfluorfen and under various environmental conditions. Both cellular leakage and lipid peroxidation caused by oxyfluorfen were found to be less in the transgenic than in the nontransgenic plants. Growth responses of the transgenic plants under various temperature, light, and water conditions were almost the same as those of the nontransgenic plants, although the transgenic plants exhibited slightly more retarded growth under low light or saturated water condition. These results revealed that the transgenic tobacco plants containing B. subtilis protoporphyrinogen oxidase gene under cauliflower mosaic virus 35S promoter were relatively resistant to oxyfluorfen and exhibited normal growth pattern. Possible mechanism of resistance to oxyfluorfen in the transgenic plants is also discussed.

• Korean Journal of Weed Science
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• v.6 no.2
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• pp.191-200
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• 1986

The study was conducted to screen paraquat-tolerant plant species among crops and weeds, using the response of plant like leaf disc discoloration, visual injury and dry weight in the presence of paraquat. Mechanism of paraquat-tolerance was investigated in strains of soybean through evaluating activities of superoxide dismutase and peroxidase and the multiplication of callus derived from soybean cotyledon. In crops, Kwanggyo has been selected as a paraquat-tolerant variety among soybean cultivars tested, and Hood as a susceptible one. In weeds, Polygonum aviculare, Chenopodium album and Pinellia ternata were evaluated as the paraquat resistant species, providing the possibility for the donor plant species for paraquat resistance. Activity of superoxide dismutase known to detoxify paraquat was markedly greater in Kwanggyo, a paraquat-tolerant cultivar than in Hood, a susceptible one. In addition, the similar response like superoxide dismutase was observed in peroxidese activity. The greater inhibition of callus multiplication was determined in Hood, a susceptible one than a tolerant one, Kwnggyo. Based on all the informations, it is strongly proposed that paraquat tolerance in soybean is due to destruction of $O_2^-$ by elevated concentration of superoxide dismutase in the tolerant cultivar.