Browse > Article
http://dx.doi.org/10.5660/KJWS.2010.30.4.380

Rapid Diagnosis of Resistance to Glufosinate-ammonium in Transgenic Sweet Potato  

Kuk, Yong-In (Dept. of Development in Resources Plants, College of Life Science and Natural Resources, Sunchon National University)
Publication Information
Korean Journal of Weed Science / v.30, no.4, 2010 , pp. 380-389 More about this Journal
Abstract
Transgenic herbicide-resistant sweet potato plants [Ipomoea batatas (L.) Lam.] produced through a biolistic transformation were used in this study. The objective of this research was to find out a rapid and reliable assay method for confirming glufosinate-ammonium resistance. The techniques tested include whole-plant bioassay, one leaf bioassay, and leaf disk bioassay. Parameters investigated in this study were leaf injury and ammonium accumulation at 1 and 5 days after treatment of glufosinate-ammonium. In the leaf disk bioassay, leaf injury of the transgenic line 7171 was 1.9-fold less affected by glufosinate-ammonium than the wild type. The leaf injury of 7171 in one leaf and whole-plant bioassays was 59- and 92-fold less affected by glufosinate-ammonium, respectively, compared with that of the wild type. Leaf disk, one leaf, and whole-plant bioassays showed that ammonium accumulation of the 7171 was 2 to 20-, 4 to 43-, and 6 to 115-fold less affected by 0.5-5 mM glufosinate-ammonium than that of the wild type. All three bioassays successfully distinguished the resistance from the transgenic lines, but one leaf bioassay is the simplest and quickest. Leaf injury and ammonium accumulation were the same in leaves 1, 3, 5, 7, and 10 of 3 mM glufosinate-ammonium treated plants or nontreated plants. The one leaf bioassay was chosen as the standard procedure for future confirmation of resistance in transgenic sweet potato because it is a rapid and reliable assay.
Keywords
glufosinate-ammonium; resistance diagnosis; transgenic sweet potato;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Choi, H. J., T. Chandrasekhar, H. Y. Lee and K-. M. Kim. 2007. Production of herbicide resistant transgenic sweet potato plants through Agrobacterium tumefaciens method. Plant Cell Tiss. Org. Cult. 91:235-242.   DOI
2 Coetzer, E., and K. Al-Khatib. 2001. Photosynthetic inhibition and ammonium accumulation in Palmer amaranth after glufosinate-ammonium application. Weed Sci. 49:454-459.   DOI
3 Culpepper, A. S., A. C. York, R. B. Batts and K. M. Jennings. 2000. Weed management in glufosinate-ammonium and glyphosate-resistant soybean (Glycine max). Weed Technol. 14:77-88.   DOI
4 De Block, M., J. Botterman, M. Vandewiele, J. Dockx, C. Thoen, V. Gossel, N. Rao Mowa, C. Thompson, M. Van Montaqu and J. Leemans. 1987. Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 6:2513-2518.
5 De Block, M., D. De Brouwer and P. Tenning. 1989. Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 91:694-701.   DOI
6 D'Halluin, K., M. De Block, J. Janssens, J. Leemans, A. Reynaerts and J. Botterman. 1992. The bar gene as a selectable marker in plant engineering. Method. Enzymol. 216:415-441.   DOI
7 Krausz, R. F., G. Kapusta, J. L. Matthews, J. L. Baldwin and J. Maschoff. 1999. Evaluation of glufosinate-ammonium-resistant corn (Zea mays) and glufosinate-ammonium : Efficacy on annual weeds. Weed Technol. 13:691-696.   DOI
8 Leason, M., D. Cunliffe, D. Parkin, P. J. Lea and B. J. Miflin. 1982. Inhibition of pea leaf glutamine synthetase by methionine sulphoximine, phosphinothricin and other glutamate analogues. Phytochemistry 21 :855-857.   DOI
9 조재영. 2007. 사정 전작. 향문사. pp. 449-508.
10 한국작물보호협회. 2010. 농약사용지침서. p. 1199.
11 Manderscheid, R., and A. Wild. 1986. Studies on the mechanism of inhibition by phosphinothricin of glutamine synthetase isolated form Triticum aestivum L. J. Plant Physiol. 123: 135-142.   DOI
12 Cao, M. X., J. Q. Huang, Z. M. Wei, Q. H. Yao, C. Z. Wan and J. A. Lu. 2004. Engineering higher yield and herbicide resistance in rice by Agrobacterium-mediated multiple gene transformation. Crop Sci. 44:2206-2213.   DOI
13 Wild, A., and R. Manderscheid. 1984. The effect of phosphinothricin on the assimilation of ammonia in plants. Z. Naturforsch 39c:500-504.
14 Wild, A., and C. Wendler. 1993. Inhibitory action of glufosinate-ammonium on photosynthesis. Z. Naturforsch 48c:367-373.
15 Wilcut, J. W., H. D. Coble, A. C. York and D. W. Monks. 1996. The niche for herbicide-resistant crops in U. S. agriculture. Pages 213-230 in S. O. Duke (ed), Herbicide-resistant crops : Agriculture, environmental, economic, regulatory and technical aspects. CRC Press, Inc., Boca Raton, FL.
16 Woolfe. J. A. 1992. Sweet potato, an untapped food resource. Cambridge University Press, New York.
17 Yi, G., Y. M. Shin, G. Choe, B. Shin, Y. S. Kim and K. M. Kim, 2007. Production of herbicide-resistant sweet potato plants transformed with the bar gene. Biotechnol. Lett. 29:669-675.   DOI
18 Yoshinaga, M., O. Yamakawa and M. Nakatani. 1999. Genotypic diversity of anthocyanins content and composition in purple-fleshed sweet potato. Breeding Sci. 49:43-47.   DOI
19 Otani. M., Y. Wakita and T. Shimada. 2003. Production of herbicide-resistant sweetpotato (Ipomoea batatas (L.) Lam.) plants by Agrobacterium tumefaciens-mediated transformation. Breed Sci . 53:145-148.   DOI
20 Manickavasagam, M., A, Ganapathi , V. R. Anbazhagan, B. Sudhakar, N. Selvaraj, A. Vasudevan and S. Kasthurirengan. 2004. Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane (Saccharum species hybrids) using axillary buds. Plant Cell Rep. 23: 134-143.
21 Petersen, J., and K. Hurle. 2001. Influence of climatic conditions and plant physiology on glufosinate-ammonium-ammonium efficacy. Weed Res. 41 : 31-39.   DOI
22 Prakash, C. S. 1994. Sweet potato biotechnology; progress and potential. Biotechnol. Dev. Mon. 18:19-22.
23 SAS Statistical Analysis System. 2000. SA/STAT User's Guide, Version 7, Statistical Analysis System Institute, Electronic Version Cary. NC.
24 Shelp, B. J., C. J. Swanton, B. G. Mersey and J. C. Hall. 1992. Glufosinate-ammonium (phosphinothricin)) inhibition of nitrogen metabolism in barley and green foxtail plants. J. Plant Physiol. 139:605-610.   DOI
25 Strauch, E., W. Wohlleben and A. Phler. 1988. Cloning of a phosphinothricin N-acetyltransferase gene from Streptomyces viridochromogenes TU494 and its expression in Streptomyces lividans and Escherichia coli. Gene 63:65-74.   DOI
26 Teow, C. C., V. D. Truong, R. F. McFeeters, R. L. Thompson, K. V. Pecota and G. C. Yencho. 2007. Antioxidant activities, phenolic and $\beta$-carotene contents of sweet potato genotypes with varying flesh colours. Food Chem. 103:829-838.   DOI