• The Journal of Korean Society of Virology
• /
• v.27 no.2
• /
• pp.105-113
• /
• 1997

The truncated $E1_{192-283}$ and $E2_{384-649}$ genes of hepatitis C virus (HCV) linked to the gene for glutathione S-transferase (GST) were constructed and their expressions were analyzed. The $GST-E1_{192-283}$ fusion gene overexpressed the fusion protein in E. coli as a soluble form, while the $GST-E1_{192-383}$ plasmid did not express expected fusion protein. The purified $GST-E1_{192-283}$ fusion protein was efficiently cleaved by thrombin. More than 90% pure, HCV $E1_{192-283}$ protein was obtained by GST-agarose chromatography. The truncated $GST-E2_{384-649}$ fusion gene expressed the fusion protein mainly as an insoluble form, whereas the $GST-E2_{384-740}$ did not express the fusion protein. The truncated $GST-E1_{182-283}$ and $GST-E2_{384-649}$ fusion proteins reacted specifically with an HCV patient serum. In addition, mice immunized with either the purified $E1_{192-283}$ or $GST-E2_{384-649}$ proteins generated specific antibodies to each antigen. The results suggested that hydrophobic carboxyl portions of the E1 and E2 proteins might affect expression levels as well as the solubility of each fusion protein in bacteria. Also, the truncated E1 protein with Tyr-192 to Ser-283 contained antigenic epitope(s) which could be specifically recognized by an HCV patient serum.

• Journal of Yeungnam Medical Science
• /
• v.15 no.1
• /
• pp.143-150
• /
• 1998

The aim of this study was to evaluate domestic enzyme immunoassay(EIA) kit 'LG RCD 3.0' (LG) for the detection of antibody to hepatitis C virus(anti-HCV) in comparision with Axsym HCV version 3.0(Axsym), Cobas Core anti-HCV EIA(Cobas). Cobas kit shows better clear distinction between positive and negative by signal/cutoff ratio(S/C), but it also reveal relatively high false positive rate. The concordance rate of test results between LG and Axsym was 96.2%, between LG and Cobas was 95.5%, and total agreement between three EIA kit was 93.9%. LG were relative poor distinction between positive and negative results, but it could be applied clinically as a screening tool for hepatitis C in general population. The SIC of one false negative result by LG was 0.91, and false positive were less than 4.0, therefore we concluded it is necessary to confirm by immunoblotting assay when SIC were between 0.8 and 4.0.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.4
• /
• pp.701-704
• /
• 2007

This report describes the full-length sequences of 2HBV clones from a hepatocellular carcinoma (HCC) patient, one with preC mutation (1896A) and the other without preC mutation. The high level of discrepancy in mutation frequency between these 2 strains was observed in the Core (C) region among 4 ORFs. These data support previous results that Korean HBV strains, belonging to genotype C2, are prone to mutations. It is possible that the mutations (BCP and preC mutations) associated with the HBeAg defective production might contribute to the diversity of mutations related to HBV persistence, playing an important role in hepatocarcinogenesis in this patient.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.7
• /
• pp.1953-1954
• /
• 2013

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.699-700
• /
• 2003

• 한국생물공학회:학술대회논문집
• /
• 2005.04a
• /
• pp.74-74
• /
• 2005

• 대한예방의학회:학술대회논문집
• /
• 1993.10a
• /
• pp.39-40
• /
• 1993

• Proceedings of the Korean Society of Life Science Conference
• /
• 2008.10a
• /
• pp.19-19
• /
• 2008

• 대한예방의학회:학술대회논문집
• /
• 2000.10a
• /
• pp.116-117
• /
• 2000

• Proceedings of the Korean Society of Life Science Conference
• /
• 2001.02a
• /
• pp.79-79
• /
• 2001