• 제목/요약/키워드: Hemolymph

검색결과 244건 처리시간 0.031초

Biochemical Performance and Quantitative Assessment of F1 Hybrid of Two Ecoraces of Tropical Tasar Silkworm Antheraea Mylitta Drury (Lepidoptera: Saturniidae)

  • Lokesh, Gangadharaiah;Tirkey, Sushma Rani;Srivastava, Ashok Kumar;Kar, Prasant Kumar;Sinha, Manoj Kumar
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • 제26권2호
    • /
    • pp.67-73
    • /
    • 2013

  • Antheraea mylitta Drury is basically a crossbreeding species, as such it seems to be potentially a good material for the exploitation of heterosis. In the present study F1 hybrid of wild ecorace Laria (L) and semi-domestic Daba (D) was raised and evaluated for various quantitative traits and biochemical parameters during larval stage. Improved fecundity ($+18{\pm}1.8%$ and higher egg hatching rate ($+10.96{\pm}1.3%$) was recorded in the F1hybrid ($L{\times}D$). Biochemical parameters studied in the hemolymph, midgut and fatbody of the larva showed significantly higher (P<0.05) total proteins and carbohydrate concentration besides digestive enzyme activity. Correspondingly SDS-PAGE revealed more number of protein bands in the hemolymph sample of F1s, ranging between 29 kDa to 66 kDa compared to parental lines. The present study demonstrates the positive heterosis effect in the F1 hybrid of Laria ${\times}$ Daba. Biochemical analysis indicates that, there is possibilities of exploitation of hybrids with specific parents targeted for desirable commercial traits (silk yield and fecundity). Moreover, most of these biochemical parameters can be used as markers to analyze the genetic improvement in the tasar silkworms.

  • https://doi.org/10.7852/ijie.2013.26.2.67 인용 PDF KSCI

Molecular Cloning and Characterization of a Vitellogenin of the Bumblebee Bombus ignitus

  • Lee, Kyung-Yong;Yoon, Hyung-Joo;Lee, Sang-Beom;Park, In-Gyun;Sohn, Hung-Dae;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • 제18권1호
    • /
    • pp.33-40
    • /
    • 2009

  • A vitellogenin cDNA was cloned from the bumblebee Bombus ignitus. The cDNA encoding B. ignitus vitellogenin (BiVg) is 5473 bases long with an open reading frame of 1773 amino acid residues. BiVg possesses two consensus (RXXR/S) cleavage sites and has the conserved DGXR and GL/ICG motif near its C-terminus. The deduced amino acid sequence of BiVg cDNA showed significant similarity with hymenopteran Vgs (51% identity to Apis mellifera Vg, and $33{\sim}36%$ to other insect Vgs). The BiVg cDNA was expressed as a 200-kDa polypeptide in baculovirus-infected insect Sf9 cells. Northern and Western blot analyses showed the expression of BiVg in fat bodies of pupae and adults. In queens, the expression of BiVg was first detected in late pupal stage fat bodies, and secreted BiVg was also observed in late pupal stage hemolymph.

  • PDF KSCI

곤충세포-배큘로바이러스 시스템에서 재조합 단백질 생산을 위한 최적 감염시기 및 배지조성 (Optimal Infection Time and Medium Composition for the Production of Recombinant Protein in Insect Cell-Baculovirus System)

  • 하성호;이성환박태현
    • KSBB Journal
    • /
    • 제10권3호
    • /
    • pp.317-322
    • /
    • 1995

  • 유전자 재조합 단백질 생산을 위한 곤충세포- 배쿨로바이러스 시스템에 있어서, 바이러스에 감염시 키는 시기가 늦을수록 재조합 단백질의 발현이 낮게 나타났다. 이것은 높은 세포농도일수록 단위세포당 낮은 발현율을 의미하므로 재조합 단백질 생산을 위 해 감염시기에 대한 최적화의 필요성을 보여주며, 재조합 단백질의 최대 생산생을 위한 최적 감염시기 의 존재를 실험적으로 업증하였다. 또한 배지에 5% 누에 체액을 보강함으로써 발현율이 증가하였고, 이 것은 누에 체액 첨가로 인해 세포내 바이러스 숫자가 증가하고 감염 후 세포의 생존성이 오래 유지되 는 것에 기 인하는 것으로 생각된다. 고농도 세포배 양을 위해 yeastolate를 첨가하고, 재조합 단백질 발 헌을 위해 누에 체액을 첨가함으로써 ${\beta}$-galactosidase의 생산이 10배 정도 증가하였다.

  • PDF