• Fisheries and Aquatic Sciences
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• v.19 no.10
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• pp.44.1-44.8
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• 2016

Rockfish, Sebastes schlegelii (mean length $14.53{\pm}1.14cm$ and mean weight $38.36{\pm}3.45g$), were exposed for 4 weeks with the different levels of ammonia in the concentrations of 0, 0.1, 0.5, and 1.0 mg/L at 19 and $24^{\circ}C$. The indicators of growth performance such as daily length gain, daily weight gain, condition factor, and hematosomatic index were significantly reduced by the ammonia exposure and high temperature. The ammonia exposure induced a significant decrease in hematological parameters, such as red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin (Hb), and hematocrit (Ht), whose trend was more remarkable at $24^{\circ}C$. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were also notably decreased by the ammonia exposure. Blood ammonia concentration was considerably increased by the ammonia concentration exposure. In the serum components, the glucose, glutamic oxalate transaminase (GOT), and glutamic pyruvate transaminase (GPT) were substantially increased by the ammonia exposure, whereas total protein was significantly decreased. But, the calcium and magnesium were not considerably changed.

• Journal of the East Asian Society of Dietary Life
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• v.7 no.2
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• pp.181-198
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• 1997

The purpose of this study was to investigated relationship between heath status and food preference of male and fermale adults who live in Iksan City, Cheonbuk, Korea. The subjects consisted of 96 male and 93 fermale adults were aged 20 to 70 years old. Each subject was interviewed to get information of food preference. Blood samples were taken concentration of serum in hemoglobin, glucose, total cholesterol, GOT(glutamic oxaloacetic transaminase), GPT(glutamic pyruvic transaminase) were measured. The results obtained were as follows: Among the food preference were like, dislike and ordinary answered to male and fermale adults the normal average of serum concentration with hemoglobin level showed 15.4, 14.2, 15.5, 12.9, 15.8 and 13.2g/dl, glucose level showed 85.8, 86.1, 87.5, 88.1, 87.9 and 86.1mg/dl, total cholesterol level showed 183.1, 185.0, 172.4, 193.5, 181.2 and 184.0mg/dl, GOT level showed 4.8, 23.4, 24.8, 23.9, 24.9 and 21.7ppm, GPT level showed 22.7, 20.2, 26.3, 18.5, 22.5 and 18.4ppm respectively. The abnormal average of serum concentration with hemoglobin level showed 11.1, 10.8, 12.2, 11.3, 12.5 and 11.0g/dl, abnormal glucose level showed 155.7 168.5, 166.2, 134.1, 124.1, 130.1, abnormal total cholesterol level showed 260.3, 273.7, 255.2, 286.5, 255.9 and 251.8mg/dl, abnormal GOT level showed 58.8, 66.8, 51.8, 50.3, 51.2 and 51.0ppm abnormal GPT level showed 54.3, 48.6, 51.3, 50.2, 53.2 and 45.5ppm respectively.

• Korean Journal of Veterinary Research
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• v.14 no.1
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• pp.9-16
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• 1974

The literature on the hematology of dairy cattle contains very important information of physiological and clinical field, but a comprehensive survey on the blood picture of Holstein cows has not been made yet in Korea. The object of the present investigation was to make good this deficiency, and to suggest standards for the blood picture of Holstein cows kept under average farming condition in this country. Observations were made on the blood picture of 30 healthy non-pregnant Holstein cows aging from 6 to 10 years. All of them were selected at random from 15 livestock farms of Deajeon area in order to determine the hematological values from January to Feburary, 1974. The ranges and mean values of erythrocyte count, hemoglobin in blood, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and total and differential leukocyte count were determined. The result obtained in this work were summerized as follows: 1. Ranges and mean values(with standard error) of erythrocyte count, hemoglobin in blood and hematocrit values were 4.90~7.82 and $5.83{\pm}0.12{\times}10^6/mm^3$, 7.8~10.3 and $8.7{\pm}0.11g/100ml$, and 26~38 and $30.2{\pm}0.53$ mI/100 ml, respectively. 2. Mean corpuscular hemoglobin, mean corpuscular volume, and mean corpuscular hemoglobin concentration showed ranges and mean values(with standard error) of 13.2~17.4 and $14.8{\pm}0.22$ pg, 46.0~71.8 and $52.3{\pm}0.31{\mu}m^3$, and 21.6~32.7 and $28.6{\pm}0.47$ g/100 ml, respectively. 3. Total leukocyte count showed a range of 6,300~13,600 and a mean value(with standard error) of $10,035{\pm}346/mm^3$. 4. Ranges and mean values(with standard error) of differential leukocyte counts of total neutrophil were 21~40 and $36.4{\pm}0.7%$, 1,638~4,080 and $3,233{\pm}111/mm^3$, of band neutrophil 0~9 and $3.9{\pm}0.5%$, 0~1,028 and $390{\pm}54/mm^3$, of segmented neutrophil 18~35 and $28.4{\pm}0.7%$, 1,386~3,672 and $2,842{\pm}103/mm^3$, of lymphocyte 46~69 and $55.3{\pm}1.1%$, 3,380~8,976 and $5,535{\pm}263/mm^3$, of monocyte 0~4 and $1.7{\pm}0.2%$, 0~324 and $116{\pm}21/mm^3$, of eosinophil 4~15 and $10.8{\pm}0.6%$, 540~1,725 and $1,082{\pm}72/mm^3$. No basophil was observed in this work.

• Journal of Nutrition and Health
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• v.28 no.10
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• pp.1022-1030
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• 1995

Saponins are glycosidic compounds present in many plant foods. They are characterized by their ability to lyse cell membranes due to their surface-active properties. Saponins are believed to interact primarily with cholesterol in the cell membrane. In this study, the interaction of soybean(SS) with cell membrane was investigated using erythrocytes as a model. Mechanisms of interaction was also investigated by measuring their binding capacity with different membrane lipid fractions. Throughout the study, gypsophilla saponin(GS) and quillaja saponin(QS) were used to evaluate the membranolytic activity of soybean saponins. All saponins released hemoglobin in a concentration-dependent manner. SS induced 40% hemolysis at the concentration of 400 ppm, however there was no increase in hemoglobin release above 400ppm concentration. 5ppm of GS and 8 ppm of QS hemolyzed 100% of erythrocytes. Isolation of SS fractions by thin layer chromatography revealed that only one non-polar saponin possesses strong hemolytic activity. When saponins were incubated decreased the release of cholesterol. When the hemolytic activity of saponins was measured in the presence of other major membrane lipid components, sphingomyelin significantly reduced the hemolytic activity of SS, while cholesterol reduced the activity of QS. GS showed high affinity to other component(s) in the incubation media as well as lipids. These results suggest that the membranolytic activity of saponins are related to their specific chemical structure, which determines the interaction behavior between saponins and different membrane components, and thereby influence the biological activity.

• Korean Journal of Veterinary Research
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• v.35 no.3
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• pp.595-601
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• 1995

Hemodynamic values were assessed in the cows diseased with mastitis. Hemodynamic testes were performed for white blood cell(WBC), red blood cell(RBC), packed cell volume(PCV), hemoglobin concentration, monocyte, eosinophil, neutrophil, lymphocyte, prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, platelet concentrations, antithrombin-III and plasminogen activities. Significant alterations were observed in the mean values of most analytes. The numbers of monocytes, eosinophil, and neutrophil, and prothrombin time were increased while the number of lymphocyte, activated partial thromboplastin time, thrombin time, fibrinogen concentration, plasminogen activity and platelet concentration were decreased. The number of RBC, PCV, hemoglobin and antithrombin-III activity were unchanged compared with normal mean values. These data indicated that activation of hemodynamic mechanisms was initiated either directly by the endotoxin-releasing or indirectly by the inflammatory mediators released by response to etiologic agents. We suspected that the changes of hemodynamic values in the cows diseased with mastitis were very similar to those of experimental endotoxin-induced mastitis.

• Korean Journal of Clinical Laboratory Science
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• v.43 no.1
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• pp.12-18
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• 2011

The purpose of this study was to evaluate the significance of higher than normal mean corpuscular volume (MCV) and lower than normal mean corpuscular hemoglobin concentration (MCHC) in 20 patients. The hemoglobin (Hb), red blood cell indices (MCV, MCH, MCHC,), red cell distribution width (RDW), serum ferritin, serum iron (Fe) and total iron binding capacity (TIBC) were measured and the transferrin saturation were calculated. 18 (90.0%) cases were categorized as chronic diseases. Chronic renal failure, malignancy, and bleeding were all related to the cases. The mean values of hemoglobin was $9.5{\pm}2.14g/dL$; the MCV was $29.0{\pm}2.8fL$; the MCH was $30.9{\pm}1.0pg$; the MCHC was $31.2{\pm}0.5%$; the RDW was $17.0{\pm}3.6%$; serum Fe was $39{\pm}21{\mu}g/dL$; the TIBC was $219.7{\pm}108.8{\mu}g/dL$; transferrin saturation was $19.2{\pm}9.9%$ and ferritin was $445.5{\pm}499.6{\mu}g/L$ in the patients. The WHO criteria for hemoglobin of patients confirms anemia in 18 of the 20 (90.0%) cases. Anemia of chronic disease was shown in 11 (73.3%) cases; acute gastric ulcer with hemorrhage in 1 (6.7%) case; iron deficiency anemia in 1 (6.7%) case; 2 patients (13.3%) were of normal cases. There were changes in the baseline Hb level results of the 19 (95.0%) cases while no change was shown in 1 case. As a result, diseases associated with anemia and bleeding where the MCV is higher than the normal range and MCHC is lower than normal range are considered relevant findings.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.4
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• pp.401-410
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• 2016

Since the late 19th century, scientific logic and techniques have been used extensively in the field of clinical pathology, including many laboratory tests utilizing various apparatuses and instruments. Among the techniques to measure hemoglobin, the visual color comparison method was most popular around this time; the specific gravity method and gasometric method were not widely adopted. Instruments that use the visual color comparison method include Gowers' hemoglobinometer, von Fleischl's hemoglobinometer, Dare's hemoglobinometer, Oliver's hemoglobinometer, Haden-Hausser hemoglobinometer, and Spencer Hb meter. Initially, the visual color comparison methods were used to diluate and hemolyze blood with distilled water and then to measure its color. Later, these methods were further developed to measure hemoglobin without dilution, and improved with the formation of acid or alkaline hematin ensuring the stability of color development. Hammerschlag's method as well as the Schmaltz and Peiper's methods were based on specific gravity measurement, but they were not widely used. The gasometric method used the Van Slyke gasometer, indirectly measuring the hemoglobin concentration. This method provides the most accurate results. This survey examined the characteristics and limitations of hemoglobinometers and methods used to measure hemoglobin from the late 19th century to the early-and mid-20th century. Moreover, this study aims to improve the understanding and applicability of the current methods and emerging technologies used in measuring hemoglobin. It is also expected that this investigation is the starting point to promote awareness of the need to organize historical data for a variety of historical relics of the diagnostic laboratory tests.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.6
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• pp.1049-1053
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• 2009

This study was carried out to investigate the effects of Eucommia ulmoides Oliver (Du-Zhong) as a main ingredient in a mixture of various medicinal herbal extracts on blood pressure, serum lipid concentrations, and hematological variables in hypertensive patients. After 4 weeks on drinking the mixed medicinal herbal teas, systolic blood pressure remarkably decreased from 167.14 to 134.86 mmHg and diastolic blood pressure decreased from 100.00 to 87.10 mmHg. In terms of serum lipid profiles, there were no differences in total-cholesterol, HDL-cholesterol, LDL-cholesterol level, and atherogenic index, However, the level of triglycerol was significantly reduced from 237.1 before the experiment to 145.00 mg/dL after 4 weeks on drinking the mixed medicinal herbal teas. Serum triglyceride were remarkably reduced from 237.1 to 145.00 mg/dL in the hypertensive patients who had taken the combined medicinal herbal extracts. Compared with data obtained from the patients before the experiment, there were no differences in hematological variables (RBC, WBC, hematocrit, hemoglobin, platlets and homocysteine) after the 4 weeks experiments, but mean corpuscular hemoglobin concentration was significantly increased by 2.70% (p<0.05) in the blood samples. Based on these findings, it can be presumed that the examined mixed medicinal herbal tea may be effective in lowering blood pressure and in increasing mean corpuscular hemoglobin concentration due to reduced serum triglyceride levels in hypertension patients.

• Journal of Nutrition and Health
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• v.15 no.4
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• pp.249-257
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• 1982

The aim of this study was to observe the relationships between the amount of menstrual blood loss (MBL) and age, parity, number of pregnancy and induced abortion, and the iron nutriture in married Korean women. Fifty-one healthy women aged 26 to 48 years were tested for their MBL, hemoglobin(Hb) concentration, hematocrit (Hct) value, mean corpuscular hemoglobin concentration (MCHC), serum iron and ferritin concentrations. The results of this study are summarized as follows : 1) The mean value and standard error of mean of the MBL was $32.0{\pm}3.94ml$, and the range of MBL was 3.8-127.2 ml for total subjects. Maximum number (23.5%) of the subjects fell in the group with MBL of 10-l9ml, while 17.7% showed MBL above 50m1. 2) There were no statistically significant differences on the mean amount of MBL between the age groups 25-48 years. 3) The mean amount of MBL in 1-2 and 3-4 para groups were 28.5 and 36.1 ml, respectively, but the difference between two groups was not significant 4) It was observed that the mean amount of MBL was gradually decreased as the number of pregnancy and induced abortion were increased, but the significance was not observed. 5) The mean values of Hb concentration, MCHC and serum iron concentration were slightly decreased in subjects with MBL of more than 50ml but the statistical significance was not observed. The mean of Hct value was not influenced by the amount of MBL. While serum ferritin concentrations were markedly decreased and the prevalence rate of anemia was markedly increased as the amount of MBL was increased. There was significant difference (P<0.05) between the mean amount of MBL with respect to the serum ferritin concentration.

• Journal of Preventive Medicine and Public Health
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• v.24 no.2 s.34
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• pp.181-194
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• 1991

This study intended to obtain an useful information on the prevalence of subjective symptoms, and to clarify the interrelationships between blood lead and lead related symptoms in low level lead exposure. The 93 male workers exposed to lead and 56 male nonexposed workers were examined for their blood lead(PBB), Zinc-protoporphy(ZPP), hemoglobin(HB) and personnal history, and completed 15 questionnaires related to symptoms of lead absorption : also measured lead concentration in air (PBA) in the workplace. The results obtained were as follows ; 1. The means of blood lead (PBB), blood ZPP and hemoglobin (HB) among workers exposed to lead were $26.1{\pm}8.8{\mu}g/dl,\;28.3{\pm}26.0{\mu}g/dl$ and $16.2{\pm}1.2g/dl$ : whereas those of nonexposed workers were $18.7{\pm}5.1{\mu}g/dl,\;20.6{\pm}8.7{\mu}g/dl$ and $17.3{\pm}1.1g/dl$. The means of above three indicies between two groups showed significant difference statistically (p<0.05). 2. The means of blood lead (PBB), blood ZPP and hemoglobin of workers exposed .to different lead concentration in air were as follows : When it was below $25{\mu}g/m^3$, the indices were $24.7{\pm}79,\;26.1{\pm}26.8{\mu}g/dl\;and\;16.4{\pm}1.1g/dl$ respectively : These indices were $27.1{\pm}8.5,\;23.9{\pm}10.92{\mu}g/dl\;and\;16.2{\pm}1.3g/dl$ when the lead concentration in air was $25{\sim}50{\mu}g/m^3$ : and they were $3.4{\pm}9.3,\;42.3{\pm}31.3{\mu}g/dl\;and\;15.5{\pm}1.2g/dl$ when the concentration of lead was above $50{\mu}g/m^3$. Although there were statistical difference in blood lead and hemoglobin among three different lead concentration in air, there was no statistical difference of blood ZPP among the three groups with different exposure levels (p>0.05). 3. The most frequent by complained symptom was 'Generalized weakness and fatigue', and fewest symptom was 'Intermittent pains in abdomen' 4. Only two symptoms out of fifteen symptoms checked by themselves revealed significant difference between exposed and nonexposed groups. These were 'Intermittent pains of abdomen' and 'Joint pain or arthralgia' (p<0.05), No positive correlation was found between the levels of blood lead and symptom groups categorized as gastrointestinal, neuromuscular and constitutional symptoms, 5. Blood lead (r=0.3995) and ZPP (r=0.2837) showed statistically significant correlation with mean lead concentration in air, whereas correlations were not demonstrated between blood lead and lead related symptoms or blood ZPP and lead related symptoms. 6. Blood lead (PBB) and ZPP showed association (r=0.2466) and the equation PBB=23.75+0.0842 ZPP was derived. 7. On stepwise multiple regression, using blood lead level as a dependent variable and ZPP, hemoglobin (HB), age, work duration (WD) and symptom prevalence as a independent variables, only ZPP significantly contributed a lot to blood lead level. 8. While the ZPP measurement was found to be a good indicator in evaluating health effect of lead absorption in low level lead exposure, lead related symptoms were not sensitive enough to evaluate of lead absorption in low level exposure.