• 대한수의학회지
• /
• 제27권1호
• /
• pp.1-7
• /
• 1987

The present studies were designed to investigate the morphology and stainability of the chicken spermatozoa. Semen samples were collected by abdominal massage from 10 cocks of Arbor, Acres strain (egg breed) and 10 cocks of white Cornish strain (meat breed). The semen samples were diluted with Sarker's solution and were washed. Some of the semen smear slides were stained with seven differential stain methods and was compared with one another by light microscope. In addition to the staining already compared, the length of heads, middle pieces and tails of 400 spermatozoa of two chicken breed was measured with micrometer. The results obtained from these, studies were as follows: 1. Eosin stain appeared to give good results than hematoxylin, pre-treated protease and eosin or hematoxylin stain, pre-treated protease and hematoxylin-eosin stain, carbol-fuchsin, stain and Giemsa 9 technique in differential staining of spermatozoal three portions and pre-treated protease and eosin stain appeared as good staining methods for middle piece of spermatozoa. 2. The average length of chicken spermatozoa was $90.4{\pm}4.0{\mu}m$, and the average length of the head, middle piece and tail of spermatozoa was $13.0{\pm}0.5{\mu}m$, $3.8{\pm}0.2{\mu}m$ and $73.6{\pm}3.8{\mu}m$ lesoectively. 3. The average length of spermatozoa of Arbor Acres strain was $89.2{\pm}5.0{\mu}m$ and the average length of the head, middle piece and tail of spermatozoa was $12.9{\pm}0.5{\mu}m$, $3.8{\pm}0.2{\mu}m$ and $72.5{\pm}4.7{\mu}m$ respectively. The average length of spermatozoa of with Cornish was $91.6{\pm}3.0{\mu}m$ and the average length of the head, middle piece and tail of spermatozoa was $13.1{\pm}0.5{\mu}m$, $3.8{\pm}0.2{\mu}m$ and $74.7{\pm}2.8{\mu}m$ respectively.

• 대한임상검사과학회지
• /
• 제39권3호
• /
• pp.223-230
• /
• 2007

Several methods have been used in the detection of Helicobacter pylori (H. pylori) which was believed to be a pathogenic organism causing chronic gastritis, benign peptic ulcer, gastric carcinoma or malignant lymphoma. Even though several methods were introduced for detection of H. pylori in stomach, there were controversies in their sensitivities and specificities. This experiment were designed to study the comparative analysis of staining methods (hematoxylin and eosin (H&E), Giemsa, Warthin-Starry and immunohistochemical stain) to dectect H. pylori in the gastric mucosa. The results were as follows. Average density score of H. pylori classified by Genta were 2.29 in Warthin-Starry stain, 2.19 in Giemsa stain, 1.34 in immunohistochemical stain and 0.98 in H&E stain. By comparison between inflammatory degree by Sydney system and result of Warthin-Starry stain, the detection rate and densities of H. pylori were increased from mild (61.5% and 0.8), moderate (90.4 and 2.1), and severe (100% and 3.2). From the above findings, Warthin-Starry stain is useful method for detection of H. pylori in gastric mucosa.

• 대한치과의사협회지
• /
• 제15권8호
• /
• pp.611-615
• /
• 1977

The author observed histochemically the nature of the calcified tissue in the rabbit dental pulp, induced by pulpal injection of potassium permanganate. The pulp of rabbit mandibular incisors were exposed and enlarged by a dental hand reamer. The exposed pulps were injected with 0.05ml of 20mM solution of potassium permanganate dissolved in Ringer's solution in experimental tooth. Also the control tooth received a pulpal injection of 0.05ml of Ringer's solution. After pulpal injection, the tooth was plugged with a gutta-percha root canal point. The staining techniques were hematoxylin-eosin stain, van Gieson stain, PAS reaction, toluidine blue stain, alcian blue-hematoxylin stain and colloidal iron-picric acid stain. The results were as follows: 1. The pulp on experimental tooth showed osteodentin-like calcified tissue. Also, in some areas, false denticle-like substance were observed. 2. The central portion of the calcified matrix showed metachromasia in toluidine blue stain had strong staining capacity in alcian blue stain. 3. The peripheral portion of the calcified tissue revealed marked van Gieson positive reaction for collagen. But their staining ability in alcian blue was slight and metachromasia was not appeared.

• 한국콘텐츠학회논문지
• /
• 제11권10호
• /
• pp.286-292
• /
• 2011

본 연구는 토끼골절모델에 미세전류자극(직류, 음극), 고전압 맥동직류 음극과 양극을 이용해 골절치유정도를 살펴보았다. 방사선 검사에 의한 육안 계측은 미세전류자극군이 고전압 맥동직류의 음극과 양극 통전군보다 골절치유척도 점수에서 통계학적으로 유의한 차이를 보였으나(p<0.05), 고전압 맥동직류 음극군과 양극군 사이에는 유의한 차이가 없었다(p>0.05). Hematoxylin-Eosin 염색과 Masson's trichrome 염색을 통한 병리조직표본 차이는 미세전류자극군에서 무층골의 증식이 다른 두 실험군보다 더 활발하게 관찰되었으며 연골내골화 과정도 다른 두 실험군에 비해 더 빠른 것으로 관찰되었다. Osteocalcin 면역조직화학 염색은 미세전류자극군이 골모세포, 골세포, 파골세포 및 골기질 내에서 면역양성반응이 다른 두 실험군보다 더 명확히 관찰되었다.

• 대한치과의사협회지
• /
• 제10권4호
• /
• pp.209-212
• /
• 1972

The effect of pyridoxine on the incisors were histochemically studied for the purpose of identifying the phosphoric acid. The microscopic observations were carried out by means of phosphoric acid reaction(Serraet Queiroz-Lopes method), mucoprotein reaction (Clara method), periodic acid-Schiff reaction (McManus method), methylen blue stain and hematoxylin-eosin stain. In the experimental result, it was found that phosphoric acid reactions of ameloblast, Tomes' process and preenamel were remarkably increased after pyridoxine administration.

• 대한치과보철학회지
• /
• 제15권1호
• /
• pp.26-33
• /
• 1977

최근 치과영역에서 자외선을 이용하는 치료방법이 많이 개발되고 있다. 자외선은 생체에 대하여 가역적 내지 불가역적인 생물학적 손상을 입히는 것으로 알려져 있다. 이에 저자는 Nuva-Lite를 이용하여 near-ultraviolet가 백서구강점막에 미치는 바를 조직학적 및 조직화학적으로 관찰하였다. 백서구강점막을 ether 마취하에 절취한후 Carnoy여과 10% 중성완충포르말린으로 고정한 후 파라핀으로 진공포매 하였다. 절편은 hematoxylin-eosin염색, Feulgen반응, methyl green-pyronin염색, alloxan Schiff반응, -SH기염색, PAS반응, colloidal iron반응, toluidine blue 염색을 실시하여 다음과 같은 결과를 얻었다. 1. 자외선 조사로 인하여 24시간 내지 3일에 극세포층에서 경미한 세포내 부종이 보였고, 6일 내지 10일에서 rete peg이 증대되었다. 2. 자외선 조사후 6시간 내지 3일에서 Feulgen반응에 양성인 DNA가 감소되는 경향이었다. 3. 자외선 조사후 -SH기 및 ${\alpha}$-amino산 반응이 상피의 상층에서 증가되는 경향이었다.

• 대한치과의사협회지
• /
• 제9권11호
• /
• pp.673-677
• /
• 1971

The buccal mucosa was observed histochemically in male rats which were injected with methionine. The staining methods were proceed by means of periodicacid-Schiff reaction, toluidine blue stain, alloxan-Schiff reaction, Mallory's aniline blue stain, Lillie's modification of Bielschowsky method and hematoxylin-eosin stain. The results of the experiment were summarized as follows: 1) PAS reactions of basement membrane and lamina propria increased after 3 and 5 days of methionine administration. 2) Metachromasia of stratum spinosum increased after 3 days of methionine administration. 3) Alloxan-Schiff reactions of stratum granulosum and stratum spinosum increased after 7 days of methionine administration. 4) In the lamina propria, aniline blue staining of collagenous fibers increased after 7, 10 and 14 days of methionine administration.

• Korean Journal of Acupuncture
• /
• 제19권1호
• /
• pp.35-45
• /
• 2002

Acupuncture and herbal medicine have been used to prevent and treat the cerebrovascular accident, such as a stroke, and many studies of acupuncture and moxibustion concerning to the stroke have been undertaken in the human and various animals. However, it was not published the protective effect of the electroacupuncture (EA) of Huan-do (GB30) on the transient forebrain ischemia injury. The neuroprotective effects of EA (2 ms, 10 Hz, and 1 - 2 mA) of GB30 on the transient forebrain ischemia injury was investigated by western blot of nNOS and hematoxylin and eosin stain in Sprague-Dawley rats. The transient forebrain ischemia injury resulted in increased expression of nNOS in the brain for 6 hrs after ischemia, and EA decreased significantly expression of nNOS protein in brain increased by transient forebrain ischemia injury. The survived neuronal cell stained with hematoxylin and eosin (H&E) decreased in the hippocampus by the 7 days after ischemia comparing with the normal. Comparing with the normal, the survived neurons seriously decreased cell in the hippocampus after the injury. However, the proportion of survived neurons was increased in EA treatment. These results suggest that EA of GB30 have neuroprotective effects on transient forebrain ischemia injury.

• 한국환경보건학회지
• /
• 제36권4호
• /
• pp.279-287
• /
• 2010

In this study we examined the effects of water extract of garlic on carbon tetrachloride-induced liver injury, and demonstrated increased beneficial enzyme and anti-oxidant activity as well as histopathological changes (by Hematoxylin-Eosin (H&E) staining, Trichrome staining, and TEM examination), and showed that the treatment was dose-dependent and safe. A total of 42 male Sprague-Dawley rats were divided equally (n=7) into six groups. To induce hepatotoxicity in these subjects, carbon tetrachloride diluted in an equal volume of olive oil was intraperitoneally administrated at 0.5 ml/kg (0.20 g/kg/day) once a day for five days. Water extract of Korean-grown garlic was administered via a stomach sonde once a day, 5 days a week, for a total of 4 weeks. Groups received 0.35 g/kg (E1), 0.70 g/kg (E2), or 1.40 g/kg (E3), with the dose adjusted for body weight. Administration of garlic extract resulted in positive physiological effects in terms of reduced oxidative stress and toxicity, and induced functional changes in the liver. Comparing the subject groups (E1, E2, E3) administered different doses of garlic extract, the importance of morphological analysis in further studies is emphasized, because morphological changes indicating hepatotoxicity could occur, even though beneficial enzyme activities were found to be elevated.

• 대한한방내과학회지
• /
• 제24권1호
• /
• pp.21-32
• /
• 2003

Objective : The aim of this study is to investigate the inhibitory effect of Injinchunggantang on hepatic sclerosis induced by $CCl_4$. Methods : Weight, liver function test and complete blood cell count, gross findings, and findings on liver tissue of the past(Hematoxylin & Eosin stain, Masson-Trichrome stain) were studied. Results : When it comes to the change of rats' body weight, The $CCl_{4^+}$Injinchunggantang group lost far less weight than The $CCl_{4^-}$only group. In the liver function test, which is focused on various areas such as total cholesterol, alkaline phosphotase, albumin, aspartate transaminase, alanine transaminase, The $CCl_{4^+}$Injinchunggantang group was much more closer to normal limit than the $CCl_4$ only group. In the complete blood cell count, including white blood cell, red blood cell, hemoglobin, hematocrite, platelet, The $CCl_{4^+}$Injinchunggantang group significantly closer to normal limit than $CCl_{4^-}$only group. In the gross findings of hepatic fibrosis models, Injinchunggantang showed inhibitory effect on hepatic fibrosis in the order. In the past findings of hepatic fibrosis models in Hematoxylin & Eosin, Masson-Trichrome staining, the liver in $CCl_{4^-}$only group showed atrophy and necrotic change with white nodules, whereas that of $CCl_{4^+}$Injinchunggantang group showed lesser significant change with the well_preserved tone of the tissue. In the extent of the inhibition of the hepatic fibrosis, the Injinchunggantang group showed statistically significant inhibitory effect(p<0.05) in the sclerosis model. Conclusions : These results show that Injinchunggantang have inhibitory effect on hepatic sclerosis induced by $CCl_4$ and further ultimately prevent liver cirrhosis. To obtain more credible results in this experiment, the invention of a new experimental model more similar to human hepatic sclerosis is still needed.