Korean Journal of Veterinary Research (대한수의학회지)
- Volume 27 Issue 1
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- Pages.1-7
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- 1987
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Studies on the Morphology and Stainability of Chicken Spermatozoa
닭 정자(精子)의 형태(形態)와 염색성(染色性) 조사(調査)
- Kwak, Soo-dong (Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University)
- 곽수동 (경상대학교 농과대학 수의학과)
- Received : 1987.01.28
- Published : 1987.04.30
Abstract
The present studies were designed to investigate the morphology and stainability of the chicken spermatozoa. Semen samples were collected by abdominal massage from 10 cocks of Arbor, Acres strain (egg breed) and 10 cocks of white Cornish strain (meat breed). The semen samples were diluted with Sarker's solution and were washed. Some of the semen smear slides were stained with seven differential stain methods and was compared with one another by light microscope. In addition to the staining already compared, the length of heads, middle pieces and tails of 400 spermatozoa of two chicken breed was measured with micrometer. The results obtained from these, studies were as follows: 1. Eosin stain appeared to give good results than hematoxylin, pre-treated protease and eosin or hematoxylin stain, pre-treated protease and hematoxylin-eosin stain, carbol-fuchsin, stain and Giemsa 9 technique in differential staining of spermatozoal three portions and pre-treated protease and eosin stain appeared as good staining methods for middle piece of spermatozoa. 2. The average length of chicken spermatozoa was
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