• 대한임상검사과학회지
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• 제39권3호
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• pp.223-230
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• 2007

Several methods have been used in the detection of Helicobacter pylori (H. pylori) which was believed to be a pathogenic organism causing chronic gastritis, benign peptic ulcer, gastric carcinoma or malignant lymphoma. Even though several methods were introduced for detection of H. pylori in stomach, there were controversies in their sensitivities and specificities. This experiment were designed to study the comparative analysis of staining methods (hematoxylin and eosin (H&E), Giemsa, Warthin-Starry and immunohistochemical stain) to dectect H. pylori in the gastric mucosa. The results were as follows. Average density score of H. pylori classified by Genta were 2.29 in Warthin-Starry stain, 2.19 in Giemsa stain, 1.34 in immunohistochemical stain and 0.98 in H&E stain. By comparison between inflammatory degree by Sydney system and result of Warthin-Starry stain, the detection rate and densities of H. pylori were increased from mild (61.5% and 0.8), moderate (90.4 and 2.1), and severe (100% and 3.2). From the above findings, Warthin-Starry stain is useful method for detection of H. pylori in gastric mucosa.

• 대한의생명과학회지
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• 제28권4호
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• pp.290-297
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• 2022

Through previous studies, the central nervous system (CNS) was collected by dividing the scuttle fly into larval, pupa, and adult stages by developmental stage, and the morphological characteristics were observed. In situ hybridization (ISH) using the collected central nervous system, it was possible to confirm the location and extent of expression of the neurotransmitter corazonin (Crz) at each stage of development. In this study, paraffin specimens were prepared using central nervous system tissues of 3rd instar larval stage of scuttle fly, which had completed in situ hybridization, and general histochemical staining (hematoxylin-eosin, H-E) and special histochemical staining (luxol fast blue-cresyl violet) was performed to observe the histological and cytological morphology characteristics of corazonin neurons. As a result, a variety of nerve cell body existed between many myelin sheath. The corazonin neurons compose cortex of central nervous system with other neurons congregating in this tissue and show larger cell body relatively in neurohistochemical analysis.

• 한국멀티미디어학회논문지
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• 제24권8호
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• pp.1000-1011
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• 2021

In recent years, using Deep Learning methods to apply for medical and biomedical image analysis has seen many advancements. In clinical, using Deep Learning-based approaches for cancer image analysis is one of the key applications for cancer detection and treatment. However, the scarcity and shortage of labeling images make the task of cancer detection and analysis difficult to reach high accuracy. In 2015, the Unet model was introduced and gained much attention from researchers in the field. The success of Unet model is the ability to produce high accuracy with very few input images. Since the development of Unet, there are many variants and modifications of Unet related architecture. This paper proposes a new approach of using Unet++ with pretrained EfficientNet as backbone architecture for breast tumor cell nuclei segmentation and uses the multi-organ transfer learning approach to segment nuclei of breast tumor cells. We attempt to experiment and evaluate the performance of the network on the MonuSeg training dataset and Triple Negative Breast Cancer (TNBC) testing dataset, both are Hematoxylin and Eosin (H & E)-stained images. The results have shown that EfficientUnet++ architecture and the multi-organ transfer learning approach had outperformed other techniques and produced notable accuracy for breast tumor cell nuclei segmentation.

• 한국환경보건학회지
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• 제36권4호
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• pp.279-287
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• 2010

In this study we examined the effects of water extract of garlic on carbon tetrachloride-induced liver injury, and demonstrated increased beneficial enzyme and anti-oxidant activity as well as histopathological changes (by Hematoxylin-Eosin (H&E) staining, Trichrome staining, and TEM examination), and showed that the treatment was dose-dependent and safe. A total of 42 male Sprague-Dawley rats were divided equally (n=7) into six groups. To induce hepatotoxicity in these subjects, carbon tetrachloride diluted in an equal volume of olive oil was intraperitoneally administrated at 0.5 ml/kg (0.20 g/kg/day) once a day for five days. Water extract of Korean-grown garlic was administered via a stomach sonde once a day, 5 days a week, for a total of 4 weeks. Groups received 0.35 g/kg (E1), 0.70 g/kg (E2), or 1.40 g/kg (E3), with the dose adjusted for body weight. Administration of garlic extract resulted in positive physiological effects in terms of reduced oxidative stress and toxicity, and induced functional changes in the liver. Comparing the subject groups (E1, E2, E3) administered different doses of garlic extract, the importance of morphological analysis in further studies is emphasized, because morphological changes indicating hepatotoxicity could occur, even though beneficial enzyme activities were found to be elevated.

• Korean Journal of Acupuncture
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• 제19권1호
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• pp.35-45
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• 2002

Acupuncture and herbal medicine have been used to prevent and treat the cerebrovascular accident, such as a stroke, and many studies of acupuncture and moxibustion concerning to the stroke have been undertaken in the human and various animals. However, it was not published the protective effect of the electroacupuncture (EA) of Huan-do (GB30) on the transient forebrain ischemia injury. The neuroprotective effects of EA (2 ms, 10 Hz, and 1 - 2 mA) of GB30 on the transient forebrain ischemia injury was investigated by western blot of nNOS and hematoxylin and eosin stain in Sprague-Dawley rats. The transient forebrain ischemia injury resulted in increased expression of nNOS in the brain for 6 hrs after ischemia, and EA decreased significantly expression of nNOS protein in brain increased by transient forebrain ischemia injury. The survived neuronal cell stained with hematoxylin and eosin (H&E) decreased in the hippocampus by the 7 days after ischemia comparing with the normal. Comparing with the normal, the survived neurons seriously decreased cell in the hippocampus after the injury. However, the proportion of survived neurons was increased in EA treatment. These results suggest that EA of GB30 have neuroprotective effects on transient forebrain ischemia injury.

• Journal of Radiation Protection and Research
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• 제25권4호
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• pp.217-221
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• 2000

소화기계 암의 효과적인 치료중의 하나로 방사선 치료법을 들 수 있으나 이 방법은 장 점막과 같이 빠르게 증식하는 조직에는 특히 부작용을 초래할 수 있다. 생쥐의 공장 융모가 감마선 전신 조사에 의해 형태학적으로 변화하는 정도를 관찰하였다. 생후 4주된 ICR계 생쥐를 6.5Gy와 12Gy를 전신 조사 3일 후에 공장을 채취하여 탈수 과정을 거쳐 파라핀을 입힌 조직을 절편기로 잘라 슬라이드에 부착시키고 일반적인 hematoxylin & Eosin 염색과정을 거쳐 염색후 광학현미경으로 관찰한 결과, 6.5Gy와 12Gy를 조사 받은 생쥐의 융모는 대체로 정상생쥐와 비교 할 때 융모의 길이가 짧아졌으며 융모의 두께가 비후되었고 내강은 넓어지는 경향을 나타내었다. 따라서 융모가 소화과정에 중요한 역할을 담당하며 방사선에 비교적 민감함을 나타내므로 방사선에 의한 생체영향 평가 뿐만아니라 방사선 방어제를 검색하는데 하나의 실험 파라메타가 충분히 될 수 있음을 인지하였다.

• 융합신호처리학회논문지
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• 제24권1호
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• pp.76-81
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• 2023

암세포와 정상세포를 구분하기 위해서는 H&E(Hematoxylin&Eosin) 염색이 필요하다. 병리 염색은 많은 비용과 시간이 필요하다. 최근 이러한 비용과 시간을 줄이고자 디지털 염색 방법이 소개되고 있다. 본 연구에서는 병리 H&E 염색의 디지털 변환 방법에 대한 새로운 알고리즘을 제안한다. 첫 번째 알고리즘은 Pair방법이다. 본 방법은 FPM(Fourier Ptychographic Microscopy)으로 촬영된 염색된 Phase 영상과 염색되지 않은 Amplitude 영상을 학습하여 염색된 Amplitude 영상으로 변환한다. 두 번째 알고리즘은 Unpair방법이다. 본 방법은 염색된 형광현미경 영상과 염색되지 않은 형광현미경 영상을 학습하여 모델링하여 디지털 염색을 수행한다. 본 연구에서는 GAN(generative Adversarial Network)를 활용하여 디지털 염색을 진행하였다. 연구 결과 Pair방법과 Unpair방법 모두 우수한 성능의 디지털 염색 결과를 확보하였다.

• 생명과학회지
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• 제22권8호
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• pp.1034-1040
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• 2012

활막조직은 관절부위에 존재하는 비연골성의 얇은 세포층으로 구성되어 있으며, 류마티스 관절염 등에서 활성화 되어진다. 우리는 골관절염(n=8)과 류마티스 관절염(n=5)에서 유래한 활막조직을 대상으로 활막조직내의 세포를 정량화하고 세포성분들을 비교 분석하고자 하였다. 활막조직을 H&E 염색한 후 광학현미경으로 관찰하였을 때 류마티스 관절염의 활막조직은 골관절염에 비해 형태적으로 두터워졌으며 비후된 양상이었다. 또한 CD68, CD90, PGP9.5 등과 마커들을 이용하여 IHC 분석을 수행한 결과 활막조직의 내막층과 내막하층에 존재하는 세포들을 특성을 분한 결과 내막층에는 대식세포가 집중적으로 분포하며, 내막하층에는 대식세포와 섬유아세포 유사 활막세포(FLS)가 존재한다는 사실을 알 수 있었다. H&E 이미지를 포토샵 프로그램을 이용하여 반전시켜 내막층과 내막하층 부위별로 세포계수 및 세포층계수를 수행하였다. 내막층 분석 결과 류마티스 관절염의 활막조직의 골괄절염보다 대식세포의 수와 층이 현저하게 증가된 것을 확인할수 있었다. 또한 류마티스 관절염의 활막조직의 내막하층분석결과 섬유아세포 유사 활막세포의 수적인 증가를 계수 할 수 있었다. 또한 류마티스 관절염의 경우 활막의 비후가 심하기 때문에 혈관의 위치가 내막층으로부터 상대적으로 멀리 위치하고 있음을 알 수 있었다. 그러므로 H&E 염색 이미지의 포토샵 분석을 이용한 골관절염과 류마티스 관절염 활막조직에 대한 정량 분석 방법은 류마티스 관절염의 발병과정에서 세포들이 활성화된다는 사실을 증명하는데 유용할 것으로 사료된다.

• 대한의생명과학회지
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• 제10권3호
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• pp.259-262
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• 2004

The aim of present study was to examine the effect of carrageenan on the tendon of skeletal muscle of rat. The tendon damage was induced by injection of carrageenan into skeletal hind muscle of rats. Rats were killed on 48 hours after carrageenan injection. The resulting tendons were fixed with 10％ neutral buffered formalin (NBF), dehydrated, embedded, sectioned by 4 μm, and stained by phosphotungstic acid hematoxylin (PTAH) or hematoxylin-eosin (H-E). Carrageenan induced the segregation of tendon fibers, intratendinous cleft, segregation of muscle cell group, wave arrangement of tendon fiber. The results suggest that carrageenan induced tendon damage of rat's skeletal muscle by morphological changes.

• 대한의생명과학회지
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• 제29권1호
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• pp.48-52
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• 2023

Formaldehyde use is associated with serious health risks, which can affect medical personnel and technicians. Therefore, we investigated the efficacy of an alternative fixative, with respect to two types of formalin fixatives, by hematoxylin and eosin (H&E) staining, periodic acid Schiff (PAS) staining, immunohistochemical (IHC) staining, and RNA extraction. For H&E staining, the circular nucleus was stained dark blue by the basic dye hematoxylin and the cytoplasm was stained red by the acid dye eosin in all three fixative samples. No difference was found in the Duksan General Science (DGS), Sigma-Aldrich, and Core-Fix fixative samples (Corebiotech) used to fix kidney tissue, after PAS staining. IHC staining showed that CD4 was significantly increased in the lippolysaccharide (LPS)-treated group compared to the control group (vehicle), confirming the changes in specific molecules. The quantity and quality of RNA from tissues fixed in the three types of fixatives were evaluated. The average concentration of RNA was 106 ng/µL and average purity at A 260/280 ratio was 1.7~2.0, regardless of fixative used. For quality of protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was confirmed by Western blotting. In conclusion, Core-Fix can be used as a fixative for pathological tissues, in histological and molecular diagnoses.